Agilent Technologies 8453 Operator's Manual page 179

Table 22
Diagnostics and Troubleshooting
Error Messages
Invalid Data Points In Spectrum
Possible Causes
Blank has higher absorbance than sample
measurement. Solvent or chemical matrix of
blank has higher absorbance than
solvent/matrix of sample.
Bubble in flow cell absorbing during blank.
Floating particle(s) in cell
Variation in sample absorbance during the
measurement process due to chemical or
physical processes.
Fluorescent sample.
Electronics failed.
Bad blank.
Action
Ensure sample and blank use the same
solvent or chemical matrix.
Measure blank on water.
In kinetics mode of ChemStation software:
1 Under Options & Information in the
Method menu, select Adjust gains
separately from blank measurement.
2 Under Set Gains in the Measure menu,
select water or air
Use cell cleaning fluid to prevent air bubbles
sticking on the window surface of the flow
cell.
Clean cell or wait untill particle(s) have
settled
Select a shorter integration time.
Use fixed gain settings in your advanced
software for the Agilent ChemStation.
This feature is not implemented in the
software of the handheld controller.
Replace the spectrophotometer data
acquisition (SDA) board.
Replace optical unit.
Repeat blank measurement.
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