The Thermal Denaturation Experiment; Running A Thermal Denaturation Experiment - Agilent Technologies 8453 Operator's Manual

3 Thermal Denaturation Mode

The Thermal Denaturation Experiment

After the temperature ramp has been defined and the method parameters
have been edited, you are able to start your thermal denaturation experiment.
In this section you will find information about the real-time online monitor of
the measurement and the Agilent ChemStation features to process your
experimental data.

Running a Thermal Denaturation Experiment

Measurements can be started either by using the measure menu from the
menu bar, by pushing the buttons (Blank, Sample, Thermal Measurement) on
the sidebar of the graphical user interface or by using selected F keys.
Before you can measure a single spectrum or perform a thermal denaturation
experiment you have to measure a Blank spectrum by selecting blank in the
Measure menu, by pressing the F4 key or by pushing the Blank button in the
SideBar of the graphical user interface.
To measure a single spectrum of the solution in the cell and display it in the
Sample Spectra window select Sample (Single Spectrum) in the measure
menu. You can also measure a single spectrum by pressing F5 or the Sample
Button in the SideBar of the graphical user interface.
Pressing F7, selecting Thermal Measurement from the measure menu or from
the SideBar of the graphical user interface displays the Sample Information
dialog box.
• Type a sample name in the Sample Name field. The sample name is included
• Type a solvent name in the Solvent field. The solvent details are included in
• Enter the molarity of the sample in mol/l in the Molarity (M) field. The
36
in the table and reports.
the Results of Trace table and reports.
molarity is included in the Results of Trace table and reports and is used in
the %G-C calculation.
Biochemical Analysis Software for Agilent ChemStation