Agilent Technologies 8453 Operator's Manual page 61

6 Press the Blank button on the front of the spectrophotometer or click Blank
7 Remember the orientation of the cell in the cell holder. Lift the level to
8 Press the Sample button on the front of your spectrophotometer or click
9 The view shows you the spectrum of your caffeine sample with a vertical
Agilent 8453 UV-visible Spectroscopy System Operator's Manual
on the Instrument Panel to start the measurement.
release the cell, remove it and flush it three times with about 1 ml of your
caffeine sample. Then fill the cell with about 3 ml of the caffeine sample.
Make sure that the cell windows are clean and reposition the cell in the
same orientation as for the reference measurement. Close the cell holder's
level.
Sample on the Instrument Panel to start the measurement.
line indicating your wavelength of interest. Below the spectrum is the
Sample/Result Table which shows the absorbance reading at 273 nm.
Using your Agilent 8453 UV-visible Spectroscopy System
Measuring Caffeine Absorbance at 273 nm
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