Correction Factors; Absolute Response Factor; Multiplier; Dilution Factor - Agilent Technologies 8453 Operator's Manual

Uv-visible spectroscopy system
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5
Quantification

Correction Factors

Correction Factors

The quantification calculations use four correction factors, the absolute
response factor, the multiplier, the dilution factor, and the sample amount.
These factors are used in the calibration procedures to compensate for
variations in detector response to different sample components,
concentrations, sample dilutions, sample amounts, and for converting units.

Absolute Response Factor

The absolute response factor for a sample component represents the amount
of the component divided by the measured area or height of the component's
peak in the analysis of a calibration mixture. The absolute response factor,
which is used by each calibrated calculation procedure, corrects for detector
response to individual sample components.

Multiplier

The multiplier is used in each calculation formula to multiply the result for
each component. The multiplier may be used to convert units to express
amounts.

Dilution Factor

The dilution factor is a number by which all calculated results are multiplied
before the report is printed. You can use the dilution factor to change the scale
of the results or correct for changes in sample composition during pre-analysis
work. You can also use the dilution factor for any other purposes that require
the use of a constant factor.
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Understanding Your Agilent ChemStation

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