Agilent Technologies 8453 Operator's Manual page 47

Uv-visible spectroscopy system
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Solvents
Your choice of solvents should be based primarily on the solvent's absorbance
characteristics over the wavelengths of interest, its suitability as a solvent for
the analyte, and on experimental conditions.
and the lower limit of their useful wavelength range.
Table 1
Lower Limit
180–195 nm
200–210 nm
210–220 nm
245–260 nm
265–275 nm
280–290 nm
Above 300 nm
Agilent 8453 UV-visible Spectroscopy System Operator's Manual
Lower Limit of UV Transmission for Some Common Solvents
Solvent
Sulfuric acid (96%)
Water
Acetonitrile
Cyclopentane
n-Hexane
Glycerol
2,2,4-Trimethylpentane
Methanol
n-Butyl alcohol
Isopropyl alcohol
Cyclohexane
Ethyl ether
Chloroform
Ethyl acetate
Methyl formate
Carbon tetrachloride
Dimethyl sulfoxide
Dimethyl formamide
Acetic acid
Benzene
Toluene
m-Xylene
Pyridine
Acetone
Carbon disulfide
Good Measurement Practices
General Considerations
Table 1
lists common solvents
3
47

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