Agilent Technologies 8453 Operator's Manual page 605

• The value in the Integration time field is the time in seconds over which the
• The number in the Interval field defines the wavelength measurement
It is recommended not to change the default value of 1 nm in order to achieve the optimum
N O T E
performance of the spectrophotometer
Select Lamp(s)... to display the Lamp(s) Parameter dialog box. It allows you to
set the parameters of the spectrophotometer's lamp(s). The format of the
Lamp(s) Parameter dialog box depends upon the spectrophotometer type.
With 8453 spectrophotometer you have the option to switch the deuterium
lamp and the tungsten lamp On and Off. The lamps can also be switched by
clicking on the lamp icons in the Instrument panel of the SideBar of the
graphical user interface.
If you change the lamp parameters, the current method is modified and the lamp status is
N O T E
changed. In the case of a lamp failure (ignition failure, lamp defect or lamp door open), the
lamp(s) parameters may be displayed as On, although the lamp(s) are off. Check the actual
status of the lamp(s) in the Spectrophotometer Status window or the Instrument panel of
the SideBar of the graphical user interface.
The actual status of the spectrophotometer instrument is summarized in the
Spectrophotometer Status window. In this window the following actual values
are displayed:
Instrument type, wavelength range, wavelength interval, integration time,
power on time, the status of the lamp(s) and the overall status of the
Agilent 8453 spectrophotometer (ready/not ready).
Biochemical Analysis Software for Agilent ChemStation
signal is collected and integrated. The Integration time must be between 0.1
and 25.5 seconds; the default is 0.5 seconds. Longer Integration times
improve the signal-to-noise ratio since signal is accumulated while noise is
averaged out. The selected integration time has a direct impact on the
minimum cycle time for a kinetic measurement. The minimum cycle time
cannot be shorter than the integration time. Hence, it might be necessary to
minimize the Integration time for fast kinetic measurements.
interval. The minimum interval is 1 nm, corresponding with the highest
resolution.
Kinetics Mode 2
15