Agilent Technologies 8453 Operator's Manual page 593

In This Guide...
The biochemical analysis software for Agilent ChemStation adds the kinetic
mode and the thermal denaturation mode to the general purpose software.
These two modes enable you to perform kinetic experiments and thermal
denaturation experiments with the Agilent 8453 spectrophotometer. This
includes capabilities to develop analytical methods, real time online monitors
to view your ongoing experiments, and tools to evaluate and process the
experimental data.
This book gives you instructions for the installation of the software and
hardware components needed for kinetic and thermal denaturation
experiments. Further, you get information about the most important software
features, which will help you to solve your analytical problems.
This manual is organized in three chapters, which lead you from the
installation of the software to the evaluation and presentation of a kinetic or a
thermal denaturation experiment.
1 Installation and Configuration
This chapter describes the installation of the Agilent ChemStation software
and the configuration of the Agilent 89090A Peltier temperature controller,
which is needed for thermal denaturation experiments.
2 Kinetics Mode
This chapter leads the user form the configuration of the spectrophotometer
and the sampling system, the development of a kinetic method to the kinetic
measurement and finally the evaluation of the experimental data.
3 Thermal Denaturation Mode
This chapter describes the setup of a temperature ramp and an analytical
method in order to perform a thermal denaturation experiment. The user will
learn about the online monitor of the experiment and the possibilities for data
evaluation with the software.
Biochemical Analysis Software for Agilent ChemStation 3