Thermal Denaturation Mode - Agilent Technologies 8453 Operator's Manual

Agilent ChemStation for UV-visible Spectroscopy
Operator's Manual for Biochemical Analysis Software
3

Thermal Denaturation Mode

Preparing a Thermal Denaturation Measurement
The Thermal Denaturation Experiment
This chapter describes the essential tools to develop a thermal denaturation
method, to acquire and evaluate the data with the thermal denaturation mode
of the biochemical analysis software. The first section,
Denaturation Measurement"
before the measurement. You will learn how to setup the spectrophotometer,
the temperature ramp for the Peltier Temperature controller and to choose
between different analytical evaluation options.
The second section, "The Thermal Denaturation Experiment"
describes the actual thermal measurement, the real-time online monitor and
the possibilities for data processing with the Agilent ChemStation software.
36
on page 28, contains information about the steps
Agilent Technologies
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"Preparing a Thermal
on page 36,
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