Agilent Technologies 8453 Operator's Manual
  1. Manuals
  2. Brands
  3. Agilent Technologies Manuals
  4. Measuring Instruments
  5. 8453
  6. Operator's manual
Agilent Technologies 8453 Operator's Manual

Agilent Technologies 8453 Operator's Manual

Uv-visible spectrophotometer
Hide thumbs Also See for 8453:
Table of Contents

Advertisement

Quick Links

Download this manual See also: Operator's Manual
Multicell Transport for
Agilent 8453 UV-visible
Spectrophotometer
Operator's Manual
s1

Advertisement

Table of Contents
loading

Related Manuals for Agilent Technologies 8453

Summary of Contents for Agilent Technologies 8453

  • Page 1 Multicell Transport for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 2 Agilent Technologies apparatus against shall not be liable for damage. errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material. Agilent Technologies Deutschland GmbH Hewlett-Packard-Strasse 8 76337 Waldbronn Germany...
  • Page 3 Multicell Transport for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual...
  • Page 4 This handbook contains specifications of the eight-position multicell transport (order number G1120A) and describes how install this accessory in an Agilent 8453 UV-visible spectrophotometer. This handbook also includes operating guidelines for checking out and using the multicell transport. For details of how to operate the multicell transport through the Agilent ChemStation, refer to the help system.

  • Page 5: Table Of Contents

    Contents 1 Description and Specifications An overview of the multicell transport accessory and its specifications Description 8 Specifications 2 Installing and Operating Your Multicell Transport How to install, operate, and do an operational checkout Site Requirements for Your Multicell Transport 13 Unpacking Your Multicell Transport 14 Installing Your Multicell Transport 15 Installing the Stirring Module 18...
  • Page 6 Contents 4 Maintenance and Repair Simple maintenance tasks, repair procedures, and adjust- ments that are necessary after you have made a repair Simple Maintenance 35 Replacing the Plastic Cover 36 Adjusting the Belt Tension 38 5 Parts and Supplies Figures and tables to help you identify part numbers of the multicell transport and related supplies...

  • Page 7: Description And Specifications

    Description and Specifications An overview of the multicell transport accessory and its specifications...

  • Page 8: Description

    Description The eight-position multicell transport (order number G1120A) is an accessory for the Agilent 8453 UV-visible spectrophotometer. It is a multiple-sample holder with multiple-position capability, see Figure 1. The multicell transport cell holder holds eight cells in a rack which can be held at a constant temperature with water circulated from a thermostatic bath (provided by the user and not supplied by Agilent Technologies).
  • Page 9 Description and Specifications Description Cell Stirring Module The cell stirring module is mounted in the multicell transport base to provide magnetic-induction stirring in the cell at or adjacent to the measurement position. The cell stirring module contains a plastic-cased magnetic impeller that may be driven by pressurized water.

  • Page 10: Specifications

    Specifications The multicell transport generally operates under the same environmental conditions as those specified in the Reference Handbook for the Agilent 8453 spectrophotometer. A maximum water pressure of approximately 3.5 bar (50 psi) can be supplied to the thermostatted cell holder and to the water-driven stirrer.

  • Page 11: Installing And Operating Your Multicell Transport

    Installing and Operating Your Multicell Transport How to install, operate, and do an operational checkout...
  • Page 12 Installing and Operating Your Multicell Transport This chapter describes the following topics: • “Site Requirements for Your Multicell Transport” on page 13 contains information about electrical requirements—it also gives recommendations for environmental conditions in the laboratory. • “Unpacking Your Multicell Transport” on page 14 contains information about what you do when the packing material is damaged or the shipment is incomplete—it also gives a list of items which come with the multicell transport accessory.

  • Page 13: Site Requirements For Your Multicell Transport

    Power Considerations There are no special considerations necessary because the multicell transport is driven from the power supply of the Agilent 8453 spectrophotometer and does not require separate line power. For power considerations of the spectrophotometer, see the Installing Your UV-visible Spectroscopy System handbook which comes with your UV-visible software or system.

  • Page 14: Unpacking Your Multicell Transport

    If the shipping container or cushioning material is damaged, notify the carrier as well as Agilent Technologies. Save the shipping material for the carrier’s inspection. Unpack the multicell transport and check the contents of the shipping container against the list below.

  • Page 15: Installing Your Multicell Transport

    Installing and Operating Your Multicell Transport Installing Your Multicell Transport Installing Your Multicell Transport Mounting the Multicell Transport 1 Place the multicell transport in the sample compartment of the spectrophotometer. Figure 2 Top View of Multicell Transport in Spectrophotometer Mounting screw 2 Move the cell holder of the multicell transport manually to the left-most position and tighten the screw at the right side.
  • Page 16 Installing and Operating Your Multicell Transport Installing Your Multicell Transport Figure 3 Connector at Rear of Multicell Transport Multicell transport connector 4 Turn on the spectrophotometer at the lower-left part of the instrument, if not already running. The transport initializes, that is, the transport moves to the HOME-position.
  • Page 17 Installing and Operating Your Multicell Transport Installing Your Multicell Transport Figure 4 Multicell Transport with Fitting Connections Inlet fitting (A) Outlet fitting (B) Tubing clamp (C) 3 Attach tubing clamps to fittings (A and B). 4 Before circulating water through the multicell transport, test the tubing arrangement by moving the cell holder to the HOME-position and cell position 8, using your operating software.

  • Page 18: Installing The Stirring Module

    Installing and Operating Your Multicell Transport Installing the Stirring Module Installing the Stirring Module The stirring module (part number G1120-60006) is an optional accessory for the multicell transport. The stirring module comprises the stirring assembly, four tubing clamps and 2 meters of 3/8 inch i.d. flexible tubing. Two positions for mounting the stirring assembly are available.
  • Page 19 Installing and Operating Your Multicell Transport Installing the Stirring Module 4 Reinsert the cell holder and screw down the cell holder screws finger tight. Connecting the Water Supply Tubing for the Stirring Module 1 Cut the tubing into two pieces of desired length. 2 Connect the tubing to the inlet and outlet fittings.

  • Page 20: Installing The Filter Kit

    Installing and Operating Your Multicell Transport Installing the Filter Kit Installing the Filter Kit Some compounds are photosensitive and may be decomposed during measurement. This effect can be reduced or eliminated by using a filter to cut off the highly energetic low-wavelength UV part of the spectrum. The filter kit (part number G1120-68707) comprises three threaded ring assemblies which hold different filters.
  • Page 21 Installing and Operating Your Multicell Transport Installing the Filter Kit Figure 7 Transmission Characteristics of Filters Roll-off Table 2 shows the type of filters available. Table 2 Filter Types and Labels Label Filter Roll-off UV roll-off 265 nm UV cut-off (at 50 %T) 295 nm UV cut-off (at 50 %T) The choice of when to use a filter and which filter to use should be made only after considering all the factors that affect your measurements.
  • Page 22 Installing and Operating Your Multicell Transport Installing the Filter Kit To Install the Filter Ring 1 Remove the multicell transport from the sample compartment. See “Removing the Multicell Transport from the Spectrophotometer” on page 25. 2 Screw the filter ring into the threaded drilling of the rear part of the multicell transport.

  • Page 23: Operating Your Multicell Transport

    Installing and Operating Your Multicell Transport Operating Your Multicell Transport Operating Your Multicell Transport Controlling Your Multicell Transport The Agilent ChemStation software for UV-visible spectroscopy offers a user interface that controls the multicell transport manually or automatically during an analysis. Refer to the help system in the software for details of how to operate your multicell transport.
  • Page 24 Installing and Operating Your Multicell Transport Operating Your Multicell Transport Helpful Hints When mixing two solutions or adding the stirring bars, bubbles may form inside the cell. Tap the outside of the cell lightly to free the trapped bubbles. If your flow cells or cuvettes are dirty and/or you observe that air bubbles stick on the window surfaces you may use the cuvette cleaning procedure, described in “Cleaning Cuvettes”...

  • Page 25: Removing The Multicell Transport From The Spectrophotometer

    Installing and Operating Your Multicell Transport Removing the Multicell Transport from the Spectrophotometer Removing the Multicell Transport from the Spectrophotometer Use this procedure in case you want to remove the multicell transport from your spectrophotometer, for example, for repairs or simply if you want to use other cuvette holders instead.

  • Page 26: To Remove The Cell Holder

    Installing and Operating Your Multicell Transport To Remove the Cell Holder To Remove the Cell Holder Loosen the two screws on the cell holder and remove the cell holder from the transport base. Figure 10 Removing the Cell Holder Assembly Cell holder screws...

  • Page 27: Diagnostics And Troubleshooting

    Diagnostics and Troubleshooting Descriptions of error messages, general troubleshooting hints, and how to check the electronics...

  • Page 28: Error Messages

    Diagnostics and Troubleshooting Error Messages Error Messages There is only one error message that can occur using the multicell transport with the Agilent ChemStation software. Multicell Transport home position not found Possible cause The multicell transport mechanism has jammed. Corrective action Check the two screws that fix the multicell transport in the spectrophotometer are not pushed up into the path of the mechanism (for example, this is the case when putting the transport beside the instrument).

  • Page 29: General Troubleshooting Hints

    Possible cause A malfunction of the spectrophotometer processor main (SPM) board, see Reference Handbook of your Agilent 8453 spectrophotometer. Carriage Does Not Move Possible cause An obstruction is preventing the carriage from moving.
  • Page 30 Diagnostics and Troubleshooting General Troubleshooting Hints carriage should slide freely. If excessive force is required to move the carriage when the belt is disconnected, either the multicell transport shafts are misaligned (or defective), or the carriage assembly is defective. To determine if the shafts are misaligned, temporarily loosen the clamp screw on the short shaft.

  • Page 31: Checking The Electronics

    Diagnostics and Troubleshooting Checking the Electronics Checking the Electronics Disconnect the multicell transport cable from the accessory connector at the rear of the spectrophotometer. Measure the resistances in the procedure below to verify that the multicell transport base is electrically functional. For pin assignment of the multicell transport cable connector, see Table 3.
  • Page 32 Diagnostics and Troubleshooting Checking the Electronics If the resistance is not correct, the cable is defective. Table 3 Multicell Transport Cable Connections Multicell Transport Cable Connector Function Jumper to 6 Motor connection Not used Motor connection Not used Jumper to 1 Switch C Switch No Switch No...

  • Page 33: Maintenance And Repair

    Maintenance and Repair Simple maintenance tasks, repair procedures, and adjustments that are necessary after you have made a repair...
  • Page 34 Maintenance and Repair This chapter describes: • simple maintenance, for example, how to clean the multicell transport and your cuvettes, • how to remove the multicell transport from the sample area of your spectrophotometer, • how to repair the multicell transport—only those replacement procedures are given which require a specific knowledge, and •...

  • Page 35: Simple Maintenance

    Maintenance and Repair Simple Maintenance Simple Maintenance Cleaning the Multicell Transport The multicell transport should be kept clean. Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent. Do not use an excessively damp cloth so that liquid can drip into the instruments.

  • Page 36: Replacing The Plastic Cover

    Maintenance and Repair Replacing the Plastic Cover Replacing the Plastic Cover If the plastic cover is defective, it should be replaced. To Remove the Plastic Cover 1 Disconnect the multicell transport. 2 Remove the multicell transport from the sample compartment. See “Removing the Multicell Transport from the Spectrophotometer”...
  • Page 37 Maintenance and Repair Replacing the Plastic Cover Rear plastic cover Front plastic cover Tubing clamp To Reassemble the Plastic Cover 1 First put the front palstic cover on the multicell transport drive. Clip together the rear and front plastic cover and close the tubing clamp. 2 Fix the plastic covers to the multicell transport drive by screwing down the six housing screws.

  • Page 38: Adjusting The Belt Tension

    Maintenance and Repair Adjusting the Belt Tension Adjusting the Belt Tension If the belt (part number 1500-1049) is losing tension, it must be readjusted. 1 Remove the multicell transport as described under “Removing the Multicell Transport from the Spectrophotometer” on page 25. 2 Remove the cell holder as described under “To Remove the Cell Holder”...

  • Page 39: Parts And Supplies

    Parts and Supplies Figures and tables to help you identify part numbers of the multicell transport and related supplies...
  • Page 40 Parts and Supplies Figure 14 Cell Holder Parts Table 4 Multicell Transport Parts Listing Item Description Part Number Cell holder G1120-62301 Plastic cover kit G1120-68708 Linear drive kit G1120-69706 Exchange part only Cable assembly G1120-81600 Item numbers refer to Figure 14...
  • Page 41 Parts and Supplies Table 5 Multicell Transport Accessories and Parts Description Part Number Stirring module kit G1120-60006 Filter assembly G1120-68707 Cell cleaning fluid 5062-8529 Tubing kit, G1120-68710 2 m of tubing and four clamps Stirring bar 9301-1161 Multicell transport adjustment tool 89075-23800 Cell holder G1120-62301...
  • Page 42 Parts and Supplies...
  • Page 43 Index Numerics errors, 28 operating conditions, 10, 13 2 mm apertures, 24 optical filter kit, 9 optical filters, cleaning, 21 filter assembly, 20 outlet fittings, 16 absorbance difference between filter kit, 9 cuvettes, 24 filter ring, 20 accessories, 41 filter wheel, 20 part numbers, 40 air bubbles, 24, 35 filters, 20...
  • Page 44 Index...
  • Page 45 Warranty Statement Warranty Statement All Chemical Analysis Products Agilent Technologies (Agilent) warrants its chemical analysis products against defects in materials and workmanship. For details of the warranty period in your country, call Agilent. During the warranty period, Agilent will, at its option, repair or replace products which prove to be defective.
  • Page 46 Warranty Statement 4 operation outside of the environmental and electrical specifications for the product, 5 improper site preparation and maintenance, or 6 customer induced contamination or leaks. THE WARRANTY SET FORTH IS EXCLUSIVE AND NO OTHER WARRANTY, WHETHER WRITTEN OR ORAL, IS EXPRESSED OR IMPLIED. AGILENT SPECIFICALLY DISCLAIMS THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE.
  • Page 47 Warranty Statement Responsibilities of Agilent Technologies Agilent Technologies will provide warranty services as described in the following table. Table 6 Warranty Services Services During Warranty Warranty Period Type Agilent CE instruments, 1 Year Onsite Agilent 1100 Series LC modules, Agilent 8453 UV-visible spectrophotometers...

  • Page 48: Safety Information

    Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. General...

  • Page 49: Safety Symbols

    Safety Information rendering first aid and resuscitation, is present. Do not replace components with power cable connected. Do not operate the instrument in the presence of flammable gases or fumes. Operation of any electrical instrument in such an environment constitutes a definite safety hazard.
  • Page 50 Solvent Information Solvent Information Observe the following recommendations on the use of solvents. Flow Cells Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair the optical properties of flow cells. Solvents Always filter solvents, small particles can permanently block capillaries. Avoid the use of the following steel-corrosive solvents: •...
  • Page 51 Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com/go/chem...
  • Page 52 Agilent Technologies on Internet...
  • Page 54 In This Book This handbook contains specifications of the eight-position multicell transport (order number G1120A) and describes how to install this accessory in an Agilent 8453 UV-visible spectrophotometer. This handbook also includes operating guidelines for checking out and using the multicell transport.

Table of Contents