Unknown Samples - Agilent Technologies 8453 Operator's Manual

7
Calibration

Unknown Samples

Unknown Samples

An unknown sample is a sample containing an unknown amount of the
compound to be quantified.
To find out how much of the compound is in the unknown sample, you must:
• create a calibration curve for the compound,
• inject an aliquot of your unknown sample and run the analysis in exactly
• determine from the signal the response, which is the area or height of the
• use the calibration curve to calculate the amount of the compound in the
For example, if the area of peak in the unknown sample is 500, you can
determine that the amount in the unknown is 5 ng/µl, by using the calibration
curve shown in
Figure 33
146
the same way as for the calibration sample,
peak due to the unknown amount of the compound, and
unknown sample.
Figure 33
Signal from Unknown Sample and Calibration Curve
on page 146.
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