General Definitions; Void Volume; Retention Time Of Unretained Compound T (M) [Min] - Agilent Technologies 8453 Operator's Manual

General Definitions

Void Volume

where:
d = diameter of column [cm]
π = constant, ratio of circumference to diameter of a circle
l = length of column [cm]
f = fraction of column volume that is not taken up by stationary phase but
available for mobile phase; default value for f = 0.68 (for Hypersil)

Retention Time of Unretained Compound t (m) [min]

(Also referred to as dead time or void time)
where:
F = flow rate of LC [ml/min]
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General Definitions

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