Reporting Results; Uncalibrated Reports; Calibrated Reports; External Standard Report - Agilent Technologies 8453 Operator's Manual

Uv-visible spectroscopy system
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Reporting Results

Two types of reports are available:
• an uncalibrated report which does not correct for detector response, and
• a calibrated report that shows results corrected for the difference in the

Uncalibrated Reports

The uncalibrated reports include the Area% and Height% reports. These reports
are mainly used in the preparation of calibrated reports. They may be of value
as a final report if the amounts of compound required to produce a unit area
or height response for the compounds of interest are similar.

Calibrated Reports

Calibrated reports correct for the difference in the detector response to the
reported compounds. One or more calibration samples containing known
amounts of the reported compounds must be run under the same conditions
used for the unknown sample. The integration data from these calibration
sample(s) are used to prepare a calibration table. This is a list of
retention/migration times, amounts and responses which is used in the
generation of the report. The calibrated reports are based on two calibration
procedures called external standard and internal standard.

External Standard Report

The ESTD report lists the results with the units of your choice or with each
compound as a percentage of all the compounds present. The external
standard procedure requires that the relative injected volume of both the
Understanding Your Agilent ChemStation
detector response to various components of the sample.
Using the ChemStation Reports

Reporting Results

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