Units And Conversion Factors - Agilent Technologies 8453 Operator's Manual

Uv-visible spectroscopy system
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4
Integration
Peak Area Measurement

Units and Conversion Factors

Externally, the data contains a set of data points; they can be either sampled
data or integrated data. In the case of integrated data, each data point
corresponds to an area, which is expressed as Height × Time. In the case of
sampled data, each data point corresponds to a height.
Therefore, in the case of integrated data, height is a calculated entity, obtained
by dividing area by the time elapsed since the preceding data point. In the case
of sampled data, area is calculated by multiplying the data by the time elapsed
since the preceding data point.
The integration calculation makes use of both entities. The units carried
internally inside the integrator are: counts × milliseconds for area and counts
for height. This is done to provide a common base for integer truncations
when needed. The measurements of time, area and height are reported in real
physical units, irrespective of how they are measured, calculated and stored in
the software.
104
Figure 26
Area Measurement for Negative Peaks
Understanding Your Agilent ChemStation

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