Cyclic Recalibrations Where Each Calibration Uses A Different Vial; Bracketing Sequence That Uses Different Vials For Opening And Closing Bracket - Agilent Technologies 8453 Operator's Manual

8
Automation
Cyclic Recalibration Sequences with Multiple Vials Containing the Same Dilution of a Standard

Cyclic Recalibrations Where Each Calibration Uses a Different Vial

To ensure that every calibration vial be injected only once, the sequence must
define a sufficient number of different calibration vials, so that the
round-robin order described in the previous example is not applied. For
example, if the sequence processes 80 sample vials with recalibrations
required after every 10 samples, the sequence table must contain 80/10 + 1= 9
calibration lines for each level.
As in the previous example, the calibration lines must be adjacent sequence
lines that reference adjacent vial positions.
Bracketing Sequence that Uses Different Vials for Opening and
Closing Bracket
The same capability is available for bracketing sequences. By defining the
appropriate vial range of calibration vials, a bracketing sequence can be
defined so that different calibration vials are used for the opening and closing
brackets. In this case, too, calibration lines in the sequence must be adjacent
just like the vial positions of the calibration vials.
Whether or not the bracketing calibration vials are used in round-robin mode
or for one single injection only simply depends on the total number of
calibration vials for each level and the number of recalibrations required by
the sequence.
The following example defines 3 sample injections that are bracketed by
calibrations. The opening bracket uses a different calibration vial than the
closing bracket. Recalibrations are required after every sample injection. so
the recalibration interval has to be 1. The number of calibration lines per level
is the number of samples plus one.
Table 24 Different Vials Used for Opening and Closing Brackets
Vial No. Sample Name
1 Cal1a
2 Cal1b
3 Cal1c
198
Sample Type Method Name
Calib MethodA
Calib MethodA
Calib MethodA
No. of Inj. Lvl Upd RT
1 1 Brkt
1 1 Brkt
1 1 Brkt
Understanding Your Agilent ChemStation
Upd RF Interval
Brkt 1
Brkt 1
Brkt 1