Agilent Technologies 8453 Operator's Manual page 624

3 Thermal Denaturation Mode
Select Temperature & Options from the method menu to open the
Temperature & Options dialog box. In this dialog box you have the possibility
to choose between several options for your thermal denaturation experiment.
• The Temperature Unit group allows you to choose the units of temperature
• Choose On in the Stirrer group to switch on the stirrer. When the stirrer is
• In the On-line trace monitor group you can specify the absorbance scale
• In the Idle Temperature group you can edit the temperature that is set
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measurement and control. You have the options to choose Celsius (°C),
Kelvin (K), or Fahrenheit (°F).
switched on, you can set the speed in rpm by typing it in the Speed field.
(y-axis) of the online trace monitor. Choose Auto Scaling to allow the
ChemStation to calculate the absorbance scale based on the signal
intensities or Choose Fixed Scaling define a fixed absorbance scale. When
you select Fixed Scaling, you can define the lower and upper limits of the
absorbance scale by typing them in the From and To fields.
between thermal denaturation experiments.
Biochemical Analysis Software for Agilent ChemStation