Agilent Technologies 8453 User Manual, Installing And Operating Manual
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Agilent Technologies 8453 User Manual, Installing And Operating Manual

Dissolution testing system
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  • Page 1 Agilent 8453 Dissolution Testing System Installing and Operating Manual Agilent Technologies...
  • Page 2 Use, duplication or disclo- sure of Software is subject to Agilent Tech- nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 3 What is Dissolution Testing? The dissolution testing software for the Agilent 8453 UV-visible spectrophotometer and Agilent ChemStation automates totally the process of measuring the rates of release of active ingredients from formulations into solutions.
  • Page 4 See your packing check list for your system options. You use the system with your dissolution vessels and bath. What You Install • Agilent 8453 UV-visible spectroscopy system • Dissolution sampling system The chapters that follow describe the installation procedures for dissolution sampling systems. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 5 This chapter describes the site requirements and contains the specifications for all units applied. Setting Up This chapter describes how to set up your Agilent 8453 UV-visible spectroscopy system and dissolution sampling system. Using the Peristaltic Pumps 1VS and 8VS This chapter describes how to install, operate, maintain and service the peristaltic pumps.
  • Page 6 Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 7: Table Of Contents

    Installing a Multicell-transport-based Sampling System Installing Your Eight-position Multicell Transport Installing Your Peristaltic Pump 8VS Connecting Multicell System Tubing Troubleshooting Part Numbers Installing a Valve-based Sampling System Setting Up Your Valve-pump Controller Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 8 Operating Your Pump Adjusting Flow Rate Maintaining Your Pump Removing the Tubing Cassette Changing the Pressure-wheel Assembly Changing the Drive Belt Changing the Fuse Servicing Your Pump Electronics Description Checking Remote Operation Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 9 Servicing Your Valve System Electronics Overview Troubleshooting Cables and Connectors Parts for Dissolution Testing Parts for Dissolution Testing Safety Information Safety Information General Operation Safety Symbols Agilent Technologies on Internet Index Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 10 Contents Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 11: Site Requirements And Specifications

    Agilent 8453 Dissolution Testing System Installing and Operating Manual Site Requirements and Specifications Site Requirements Specifications Agilent Technologies...

  • Page 12: Site Requirements

    100 - 120 V AC or 220 - 240 V AC. Before you connect your device to a power outlet, make sure your device version is in coincidence with your line voltage supply. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 13: Power Cords

    Use of non Agilent cables WA R N I N G The use of cables which haven't been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. • Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.

  • Page 14: Environment

    Condensation conditions must be avoided. The electronics of the devices may be damaged C A U T I O N if condensation occurs. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 15: Specifications

    Connect the pump 1VS to the valve-pump-controller only by the controller-to-pump cable N O T E (PN G1103-61612) provided by Agilent. Connect the pump 8VS to the 8453 spectrophotometer only by the GPIO-to-pump cable (PN G1103-61607) provided by Agilent Table 1...
  • Page 16 2 - 14 ml/min Pump tubing with 2.06 mm inner diameter Speed 1 - 67 rpm clock wise and counter clockwise operation Pumping pressure < 2 bar (30 psi) Pumping distance < 7 m Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 17: Valve Pump Controller

    A maximum of four valve units and four peristaltic pumps 1VS can be controlled by the valve pump controller. Connect the valve pump controller to the 8453 spectrophotometer only by the N O T E GPIO-to-controller cable (PN G1103-61610) provided by Agilent.
  • Page 18 2 bit digital output (4 connectors) TTL level Interface to valve units D-sub, 15-pin female connector 8 bit digital input/output (4 connectors) TTL level 5 V DC power supply 24 V AC power supply Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 19: Valve Unit

    -40–70 °C (-4–158 °F) temperature Savety considerations Pollution degree 2 For indoor use only Table 6 Performance Specifications Valve Unit Type Specification Comments Switching speed < 500 ms from port to adjacent port Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 20 Pumping water at 20 °C (68 °F) through each port during switching. A switching cycle is a 360° turn of the valve. Depending on your application, you may need to clean the valve at regular intervals. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 21: Setting Up

    Agilent 8453 Dissolution Testing System Installing and Operating Manual Setting Up Setting Up Your Spectrophotometer Setting Up Your Sampling System Installing a Multicell-transport-based Sampling System Installing a Valve-based Sampling System Installing Your Multibath System Agilent Technologies...

  • Page 22: Setting Up Your Spectrophotometer

    • Read about the power and site requirements for your instrument. UV-visible spectroscopy system • Unpack and set up your Agilent 8453 When you have installed your spectroscopy system, return to this handbook. N O T E Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 23: Setting Up Your Sampling System

    Table 7 shows the naming for the various fittings of the different sampling systems. Table 7 Descriptions of Fittings Parts Description Dissolution probe, including fitting and ferrule Fitting, nut Ferrule Union Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 24 Table 8 Color Coding Color Vessel Brown Vessel 1 Vessel 2 Yellow Vessel 3 Green Vessel 4 Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 25 Blank Orange Control White Common, e.g. conical adapter Black + colored band Special cell fittings You must adjust the unions to the fittings so that all the connections are leak tight. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 26: Dissolution Bath

    3 Insert the probes into your dissolution vessels. Attach the necessary fitting and tubing to the union, see “Connecting Multicell System Tubing” on page 31, and “Connecting Valve System Tubing” page 49. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 27: Installing A Multicell-Transport-Based Sampling System

    To install the multicell system you will need the following parts: • 8-Position multicell transport • Peristaltic pump 8VS • Multicell tubing kit • Flow cells, 1 mm path length (8) • Peristaltic pump–remote cable • Dissolution probe kit Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 28: Installing Your Eight-Position Multicell Transport

    N O T E Position 8 is at the right side of the multicell transport. Installing Your Eight-position Multicell Transport To install your eight-position multicell transport in the Agilent 8453 spectrophotometer, refer to your Multicell Transport for Agilent 8453 UV-visible Spectrophotometer Operator’s Manual.
  • Page 29 Setting Up Installing a Multicell-transport-based Sampling System 4 You connect the multichannel pump to the Agilent 8453 spectrophotometer with the multichannel pump–remote cable (part number G1103-61607). 5 Push connector of the peristaltic pump–remote cable (part number G1103-61607) into the remote control connector on the rear of the pump.
  • Page 30 Figure 4 Tubing-Cassette in Position 3 Repeat for each of the cassettes. More detailed information on using the pump can be found in Chapter “Using the Peristaltic Pumps 1VS and 8VS”. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 31: Connecting Multicell System Tubing

    Table 8 on page 24. 1 Insert the flow cells into the multicell transport in the Agilent 8453 spectrophotometer. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 32 Tubing to outlet on flow cell Colored heat-shrunk sheath on tubing Fitting Conical adaptor Pump tubing Figure 6 Connecting Flow Cell to Pump Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 33 6 Insert a conical adapter into the pump tubing on the outlet side of the pump. 7 Insert the open ends of the tubing into the vessels. 8 Bundle your tubing together with the spiral tubing wrap to keep it from kinking. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 34: Troubleshooting

    Measure zero cells on water to compensate for solution. different absorbancies to the windows. Specify individual path lengths in the Agilent ChemStation software to compensate for different path lengths of the cells. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 35: Part Numbers

    UV-visible section of Agilent’s Accessories and Supplies for UV-visible Spectroscopy Cata- log. † Supplied with pre assembled tubing and fittings. ‡ Supplied pre assembled. Table 7 on page 23 for details of fittings. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 36: Installing A Valve-Based Sampling System

    To install the valve system you will need the following parts: • Valve system tubing kit • Flow cell, 1 mm path length • Valve-pump controller • Eight-port valve unit • Peristaltic pump 1VS Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 37 Setting Up Installing a Valve-based Sampling System • Controller–GPIO cable • Controller–valve cable • Controller–pump cable • Dissolution probe kit Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 38: Setting Up Your Valve-Pump Controller

    Each valve-pump controller is identified by a 10-digit serial number on a label attached to the base. Write the serial number of the valve-pump controller here: _____________________________________________________________________ Line power switch Status lamps for pumps Figure 9 Front View of Valve-Pump Controller Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 39 WA R N I N G your line-power supply, see Figure 10 Table 11 for details. Table 11 Voltage Selection Power Supply Select 100–120 V AC 115 V AC 220–240 V AC 230 V AC Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 40 1 Insert the tip of small screwdriver under the slot on the cover of the line-power socket to access the fuse. Socket cover Slot Figure 11 Power Socket of Valve-Pump Controller 2 Use a screwdriver or your finger to lift out the fuse holder. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 41 Installing a Valve-based Sampling System 3 Remove the fuse and replace with a new one. A spare fuse is located in the fuse holder. 4 Replace the fuse holder. 5 Close the socket cover. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 42: Setting Up Your Valve Unit

    Each valve unit is identified by a 10-digit serial number on a label attached to the rear panel. Write the serial number of the valve unit here: _____________________________________________________________________ Valve drive assembly Channel display Status lamp Valve Figure 12 Front View of Valve Unit Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 43 Do not place the valve unit above ventilation slits of other electrical appliances. If you are using flammable or toxic solvents, place the valve unit in a safe container. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 44: Connecting Cables

    Valve unit Controle GPIO-to-controller cable Figure 14 Connecting Cables The valve-pump controller can control up to four valve units and pumps. You connect the valve unit and its pump to channel 1. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 45 More detailed information on using the valve-pump controller and eight-port valve can be found in Chapter 4, “Using the Valve Unit and Valve-pump Controller”. You set up your dissolution vessels with the probes, see “Dissolution Bath” page 26. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 46: Installing Your Peristaltic Pump 1Vs

    5 You connect the peristaltic pump to the valve-pump controller (use connector marked pump 1) with the controller-pump cable (part number G1103-61612). 6 Set the flow control to 60 for a flow rate of about 8.5 ml/min. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 47 2 Repeat for each of the cassettes. Stage 3: Installing the Tubing Cassette 1 Put the tubing cassette on the pressure-wheel assembly by placing the cassette on the mounting pivot, see Figure 15). Figure 15 Placing Tubing-Cassette Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 48 2 Press the cassette down to lock the right side onto the locking-bar. Figure 16 Tubing-Cassette in Position More detailed information on using the pump can be found in Chapter “Using the Peristaltic Pumps 1VS and 8VS”. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 49: Connecting Valve System Tubing

    You identify the different tubing by the fittings, the colored heat-shrunk sheaths and the lengths. To connect valve system tubing: 1 Insert the flow cell into the standard cell holder in the Agilent 8453 spectrophotometer. 2 Connect the dissolution probes to the valve unit, see...

  • Page 50: Replacing Tubing

    Replacing Tubing General instructions for attaching new fittings to tubing are provided in a replacement PTFE tubing kit (part number 5041-2191). This kit contains 10 m of PTFE tubing. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 51: Troubleshooting

    Change filter tips and/or use filter tips with a wider pore size, if appropriate. Leaking valve. Check your valve according to the procedure given in Chapter 4, “Using the Valve Unit and Valve-pump Controller”. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 52: Part Numbers

    UV-visible section of Agilent’s Accessories and Supplies for UV-visible Spectroscopy Cata- log. † Supplied with pre assembled tubing and fittings. ‡ Supplied pre assembled. Table 7 on page 23 for details of fittings. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 53: Installing Your Multibath System

    Each time a sample is taken a certain amount of dissolution medium is lost to waste. Figure 18 Multibath System (G1129A) Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 54: Installing Your Multicell Transport

    • Dissolution probe kit (2) • Valve tubing kit (2) • Pump tubing (2) • Filter tips (2) Installing Your Multicell Transport For details, see Installing and Operating Your Eight-position Multicell Transport manual. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 55: Setting Up Your Valve-Pump Controller

    Each valve-pump controller is identified by a 10-digit serial number on a label attached to the base. Write the serial number of the valve-pump controller here: _____________________________________________________________________ Line power switch Status lamps for pumps Figure 19 Front View of Valve-Pump Controller Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 56 WA R N I N G your line-power supply, see Figure 10 Table 11 for details. Table 15 Voltage Selection Power Supply Select 100–120 V AC 115 V AC 220–240 V AC 230 V AC Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 57 2 Use a screwdriver or your finger to lift out the fuse holder. 3 Remove the fuse and replace with a new one. A spare fuse is located in the fuse holder. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 58: Setting Up Your Valve Units

    Write the serial numbers of the valve units here: 1.)__________________________________________________________________ 2.)__________________________________________________________________ 3.)__________________________________________________________________ 4.)__________________________________________________________________ Valve drive assembly Channel display Status lamp Valve Figure 22 Front View of Valve Unit Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 59 Do not place the valve unit above ventilation slits of other electrical appliances. If you are using flammable or toxic solvents, place the valve unit in a safe container. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 60: Connecting Cables

    Controle GPIO-to-controller cable Figure 24 Connecting Cables The valve-pump controller can control up to four valve units and pumps. You connect the valve unit and its pump to the same channel. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 61 More detailed information on using the valve-pump controller and eight-port valve can be found in Chapter 4, “Using the Valve Unit and Valve-pump Controller”. You set up your dissolution vessels with the probes, see “Dissolution Bath” page 26. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 62: Installing Your Peristaltic Pump 1Vs

    1 Set the correct voltage option (115 V or 230 V) for operation from your line power supply. 2 Check the correct fuse is installed. 3 Push connector of power cord into line-power socket. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 63 Make sure that the fixing-collars are pointing upwards and the tubing cannot slide out of the cassette. 2 Repeat for each of the cassettes. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 64 2 Press the cassette down to lock the right side onto the locking-bar. Figure 26 Tubing-Cassette in Position More detailed information on using the pump can be found in Chapter “Using the Peristaltic Pumps 1VS and 8VS”. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 65: Connecting Valve System Tubing

    Installing Your Multibath System Connecting Valve System Tubing You use the multicell transport in the Agilent 8453 spectrophotometer with the valve-based multibath system. The direction of flow for your samples is from the dissolution vessels through the valve to the flow cell in the cell holder through the pump tubing to waste.

  • Page 66: Replacing Tubing

    Replacing Tubing General instructions for attaching new fittings to tubing are provided in a replacement PTFE tubing kit (part number 5041-2191). This kit contains 10 m of PTFE tubing. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 67: Troubleshooting

    Change filter tips and/or use filter tips with a wider pore size, if appropriate. Leaking valve. Check your valve according to the procedure given in Chapter 4, “Using the Valve Unit and Valve-pump Controller”. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 68: Part Numbers

    UV-visible section of Agilent’s Accessories and Supplies for UV-visible Spectroscopy Cata- log. † Supplied with pre assembled tubing and fittings. ‡ Supplied pre assembled. Table 7 on page 23 for details of fittings. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 69: Using The Peristaltic Pumps 1Vs And 8Vs

    Agilent 8453 Dissolution Testing System Installing and Operating Manual Using the Peristaltic Pumps 1VS and Operating Your Pump Maintaining Your Pump Servicing Your Pump Agilent Technologies...

  • Page 70: Installing Your Pump

    • controls, • cable connectors, and • cassettes. Controls The front panel controls are a line power switch, a three-position control switch, a flow control switch, and a safety symbol. See Figure Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 71 The safety symbol indicates a potential hazard to the user and pump during operation. Cable Connectors The rear panel has a power socket, a remote cable connector, a line voltage selector, and a fan outlet. See Figure Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 72 Rear View of the Pump Cassettes The tubing-cassette has automatic pressure control, and slots for the tubing with locating holes for the fixing-collars. See Figure Fixing collar Fixing collar Figure 30 Tubing-Cassette Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 73: Operating Your Pump

    8 Reconnect the peristaltic pump remote cable to the remote connector to enable remote operation from the Agilent 8453 spectrophotometer. For operating the pump through the peristaltic pump remote cable from the Agilent 8453 spectrophotometer, see the appropriate manual. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 74: Maintaining Your Pump

    Peristaltic pump drive assembly, 230 V 89092-69002 Peristaltic pump pressure-wheel assembly 8 89092-60003 channel Drive belt 89092-60004 T500 mA fuse for 230 V AC operation 2110-0458 T1 A fuse for 115 V AC operation 2110-0457 Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 75: Removing The Tubing Cassette

    74 for the part numbers for exchanging the pressure-wheel assembly and drive assembly. For exchanging either part you remove the pressure-wheel assembly. You require a 3 mm hex key to remove pressure wheel. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 76 2 Screw the three screws through the pressure-wheel assembly to the front of the pump, using a 3 mm hex key. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 77: Changing The Drive Belt

    WA R N I N G prevent damage always use an ESD protection (for example, an ESD wrist strap) when handling electronic boards and components. Figure 32 Lifting Pump Housing Cover Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 78 33), at the same time turning the toothed-drive wheel of the gear (Figure 33). 2 Remove the drive belt from the toothed-wheel of the gear box. Figure 33 Drive Belt of the Pump Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 79: Changing The Fuse

    Make sure line-power cord is disconnected before installing or replacing a fuse. WA R N I N G 1 Use a screwdriver to open the fuse cover. 2 Remove the fuse and replace with a new one. 3 Close the fuse cover. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 80: Servicing Your Pump

    Using the Peristaltic Pumps 1VS and 8VS Servicing Your Pump Servicing Your Pump This chapter is for Agilent Technologies service personnel. It contains a description of the electronics and procedure for checking remote operation of the pump. Electronics Description A description of the parts in Figure 34 follows in the next sections.
  • Page 81 For manual operation the direction switch, on the front panel, controls the direction of the pump through the direction relays. This switch is operational only when the remote cable is not connected. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 82: Checking Remote Operation

    Select a direction for pumping by pressing the switch. c Set the flow control switch to a value greater than zero. 3 Reconnect the peristaltic pump remote cable, the operation of the pump stops. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 83 15-pin male connector for the pump. The remote cable for the peristaltic pump 8VS is a shielded cable with a 15-pin connector for the GP-IO connector at the Agilent 8453 spectrophotometer, and a 15-pin male connector for the pump.
  • Page 84 Pin Number Pin Number Ground 2, 1–2 connected Remote enable (-) Pump On, CCW (-) Direction, CW (-) * (-) = active low. The other pins in the cable are not connected. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 85: Using The Valve Unit And Valve-Pump Controller

    Agilent 8453 Dissolution Testing System Installing and Operating Manual Using the Valve Unit and Valve-pump Controller Operating Your Valve System Troubleshooting Your Valve System Part Numbers Servicing Your Valve System Agilent Technologies...

  • Page 86: Operating Your Valve System

    The status lamp on the front of the valve unit is on when the valve unit is in remote mode. You use remote mode to operate the valve unit through your software. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 87: Operating Your Pump

    You cannot use the switches on the front panel of the pump. When the pump is on and pumping solvent, the valve-pump controller's status lamps display the pump channel that is pumping. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 88: Troubleshooting Your Valve System

    Call Agilent Technologies. b If valve unit does not operate in manual mode: Remove valve, see “Changing Valve” on page 92, and check manual operation of the valve drive assembly. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 89 Place and hold a screwdriver in the slot on the rotor drive to provide mechanical resistance. Do not turn the screwdriver. If the valve drive assembly does not operate, call Agilent Technologies. If the valve drive assembly operates, the valve has stuck. Clean the valve, “Cleaning Tubing and Valve”...
  • Page 90 The pumping time depends on the length of tubing. 7 Compare the results with the solvent reference. An absorbance of not more than 0.005 AU for the solvent indicates less than 0.5% cross contamination. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 91: Solving Your Problem

    2 Pump solvent though the tubing and the valve for a few minutes. 3 Move the valve to the next position. 4 Pump solvent again and change the valve position until all ports have been cleaned. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 92 4 Remove the valve. You may have to move the valve left, right, up or down to release it from the shaft. Valve drive assembly Valve Screws Figure 35 Removing the Valve Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 93 The shaft has some lateral movement because of its coupling. N O T E Replace and tighten the two screws. Turn on valve-pump controller and check operation. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 94 2 Slide a fitting over the end of the tubing. 3 Slide an o-ring over the end of the tubing—the inside of the o-rings are beveled, make sure the narrow end of the bevel points towards the fitting, Figure Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 95 Tightening the Fittings Always hold the nut extender tool at the end with the finger grip—optimum tightness is N O T E reached when the nut extender tool begins to slip. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 96: Part Numbers

    T315 mA fuse for 230 V AC operation 2110-0449 T630 mA fuse for 115 V AC operation 2110-0450 T1 A fuse (24 V) 2110-0457 T2 A fuse (5 V) 2110-0601 ‡ Fittings Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 97 † Does not include valve. ‡ For part numbers of cell fittings and ferrules, tubing fittings, ferrules, conical adapters and unions, see the UV-visible section of Agilent’s Accessories and Supplies for UV-visible Spectroscopy Cata- log. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 98: Servicing Your Valve System

    Using the Valve Unit and Valve-pump Controller Servicing Your Valve System Servicing Your Valve System This section is intended only for use by Agilent Technologies service personnel. It contains a description of the electronics and troubleshooting procedures. Logic levels of voltages are given as 0 for low level (approximately 0.7 V) and 1 for high level (approximately 4.8 V).

  • Page 99: Electronics Overview

    Agilent Agilent 89079A 89079A 89079A 89079A pump 1 pump 2 pump 3 pump 4 power on reset detector line 5VDC power filter supply (fuse) 24VAC line switch Figure 40 Electronics Overview Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 100 The + 5 V DC line is regulated with a linear voltage regulator. The 24 V AC lines are used to drive the valve motors (only one motor is driven at a time to minimize power consumption). Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 101 These fuses are located inside the valve-pump controller on the rear panel connector board, see Figure F1: T2000 mA (2110-0601) for the 5 V DC line. F2: T1000 mA (2110-0457) for the 24 V AC line. Figure 41 Internal Fuses Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 102 A “0” on the read/write line indicates a write cycle of the spectrophotometer, that is address and data are written into the valve-pump controller, see Figure Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 103 44. It is “0” when the valve-pump controller is ready to deliver position feedback data from the valve of the channel which is addressed. address R/W (-) DV (-) data rdy (-) Figure 44 GP-IO Read Cycle Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 104 The actual position is given to the spectrophotometer on the data-out lines DXOUT through buffer U1 in case of a read cycle. Table 26 Valve Position Lines (assuming the valve unit is connected and addressed) D2OUT D1OUT D0OUT D2IN D1IN D0IN Position Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 105 (position line is “1” if setpoint position is reached). (These lines can be accessed, opening the valve-pump controller-valve cable.) Table 27 Encoder and Position Lines Encoder Encoder Encoder Bit 2 Bit 1 Bit 0 Position Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 106: Troubleshooting

    ✔ Check if spectrophotometer and valve-pump controller electronics work: Observe if the channel LED on the valve-pump controller lights when pump is activated from the software. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 107 Pin 3 Pump off CCW operation CW operation ✔ Check the electronics of the GP-IO port of the spectrophotometer, refer to “Checking GP-IO Output Signals of the Spectrophotometer” on page 109. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 108 (Encoder Bit 0, 1 and 2 are open, which simulates position 8). As soon as the position line is pulled high, the run line Run(-) must be high. 4 If the Run(-) line does not respond replace the valve-pump controller. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 109 Check the read/write line R/W(-), data valid line DV(-) and ready line Rdy(-), refer to Figure 44 on page 103. (Your measuring device must be able to detect logic level transitions.) Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 110: Cables And Connectors

    Encoder Bit 1 Encoder Bit 2 Position Run(-) 14, 15 24 V AC * (-) means active low. Table 31 GPIO-to-controller Cable (G1103-61610) Spectrophotometer Valve-pump Controller D0IN D1IN D2IN R/W(-) † DV(-) Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 111 ‡ (-) means active low. Table 32 Controller-to-Pump Cable (G1103-61612) Pump Valve-pump Controller 1, 2 Direction, CW Pump ON, CCW * Open collector output up to 500 mA at low level. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 112 Using the Valve Unit and Valve-pump Controller Servicing Your Valve System Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 113: Parts For Dissolution Testing

    Agilent 8453 Dissolution Testing System Installing and Operating Manual Parts for Dissolution Testing Parts for Dissolution Testing Agilent Technologies...

  • Page 114: Parts For Dissolution Testing

    * For part numbers of cell fittings and ferrules, tubing fittings, ferrules, conical adapters and unions, see the UV-visible section of Agilent’s Accessories and Supplies for UV-visible Spectroscopy Cata- log. † Supplied with pre assembled tubing and fittings. ‡ Supplied pre assembled. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 115 Table 36 Ordering Information for Replacement Parts and Tubing Kits Description Part Number Filter tips (pack of 1000) 5181-1246 Flow cell, 1 mm path length 5063-6570 † Sipper/sampler tubing kit 5042-1334 Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 116 Table 7 on page 23 for details of fittings. Table 37 Handbooks Description Part Number Agilent 8453 UV-visible Spectroscopy System G1115-90034 Installation Guide Agilent 8453 UV-visible Spectroscopy System G1103-90004 Service Manual Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 117 Parts for Dissolution Testing Table 37 Handbooks (continued) Description Part Number Agilent ChemStation for UV-visible Spectroscopy: Systems G1118-90011 Understanding Your Dissolution Testing Software Multicell Transport for Agilent 8453 UV-visible Spectrophotometer G1120-90001 Operator’s Manual Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 118 Parts for Dissolution Testing Parts for Dissolution Testing Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 119: Safety Information

    Agilent 8453 Dissolution Testing System Installing and Operating Manual Safety Information Safety Information Agilent Technologies on Internet Agilent Technologies...

  • Page 120: Safety Information

    Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 121: Safety Symbols

    The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect the apparatus against damage. Indicates dangerous voltages. Indicates a protected ground terminal. Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 122 A caution alerts you to situations that could cause a possible loss of data. Do not C A U T I O N proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 123: Agilent Technologies On Internet

    Safety Information Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com/chem Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 124 Safety Information Agilent Technologies on Internet Agilent 8453 Dissolution Testing System Installing and Operating Manual...

  • Page 125: Index

    15, pump, 39, spectrophotometer, 12, pump pump remote, 45, valve, connecting, 44, remote, 39, connector, 39, valve, 39, peristaltic, 46, valve-pump controller, 43, Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 126 15, 17, 18, wavelength spectrophotometer range, connecting, 44, weight, 15, spiral tubing wrap, width of slit, status lamps, 38, stray light, switch remote/manual, 43, switching remote to manual, temperature operating, 15, 17, Agilent 8453 Dissolution Testing System Installing and Operating Manual...
  • Page 128 • Chapter 2, “Setting Up” — How to set up your Agilent 8453 UV-visible spectroscopy system and dissolution sampling system. • Chapter 3, “Using the Peristaltic Pumps 1VS and 8VS” — How to install, operate, maintain and service the peristaltic pumps.

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