Agilent Technologies 8453 Operator's Manual page 400

5
Quantification
ISTD Calculation
2 Use a calibrated peak. This ensures that the same response factor is used
The equations used to calculate the actual amount of an uncalibrated peak x
are shown above.
126
RF is the Response Factor set in the Calibration Settings dialog box.
x
You can see from these formulae that the variations in the ISTD response
are used to correct the quantification of the unknown component.
for the quantification of all peaks. The response factor of the selected
compound and the uncalibrated peaks is corrected during all
recalibrations. If the calibrated peak response factor changes, then the
response factor for the unidentified peaks also changes by the same
amount. If a Calibration Table is already set up, you can select a compound
from the Using Compound combo box in the Calibration Settings dialog box.
Understanding Your Agilent ChemStation