Agilent Technologies 8453 Operator's Manual page 91

Analysis
1 Remember the orientation of the cell in the cell holder. Lift the level to
2 Press the Sample button on the front of your spectrophotometer or click
3 Enter your sample's information in the Sample Information dialog box and
Agilent 8453 UV-visible Spectroscopy System Operator's Manual
release the cell, remove it and flush it three times with about 1 ml of your
caffeine sample (1:1 dilution with distilled water). Then fill the cell with
about 3 ml of the caffeine sample. Make sure that the cell windows are clean
and reposition the cell in the same orientation as for the standard
measurement. Close the cell holder's level.
Sample on the Instrument Panel to start the measurement.
click OK. The view switches to the samples and your quantitative results
will be displayed with the Sample/Result table.
Using your Agilent 8453 UV-visible Spectroscopy System
Quantitative Analysis using a Calibration with Standards
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