Automatic Recalibration - Agilent Technologies 8453 Operator's Manual

Uv-visible spectroscopy system
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Automatic Recalibration

Calibration is often done after a change in operating conditions, for example,
after changing a column or capillary. Automatic recalibration is usually done
at the start of a sequence of analyses or at regular intervals during a sequence
as part of a program to compensate for factors that affect the analytical
performance.
There are two ways to specify automatic sequence recalibration:
• explicit calibration sequences, or
• cyclic calibration sequences.
Recalibration using Preference Mode "Unique Folder Creation ON"
While performing a recalibration, the calibration table of the used method is
update according the defined method settings. Using the data storage mode
"Unique Folder Creation ON", the recalibrated methods are available within
the sequence data container. The calibration table of the sequence method is
updated during that process. In addition the DA.M method of the individual
data files contains the updated calibration used for result creation.
Recalibration using Preference Mode "Unique Folder Creation OFF"
While performing a recalibration, the calibration table of the used method is
update according the defined method settings. Using the data storage mode
"Unique Folder Creation OFF", the calibration table of the master method is
update during the recalibration.
Understanding Your Agilent ChemStation
Automation

Automatic Recalibration

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