Agilent Technologies 8453 Operator's Manual page 6

Contents
3 Good Measurement Practices
General Considerations
Inserting a Cell
4 Using your Agilent 8453 UV-visible Spectroscopy System
Starting Your First Measurement Session
Starting Your UV-visible Software
Measuring Caffeine Absorbance at 273 nm
Saving Your Parameters as a Method
Retrieving and Printing a Method
Saving and Retrieving Data
Print Preview of Reports
Finding the Caffeine Absorbance Maximum
6
Spectrophotometer Design
Making Measurements
Sample Cell Material
Optical Specifications of Cells
Apertured Cells 43
Flow Cells 44
Handling and Maintaining Cells
Solvents 47
Sample Preparation
Photosensitive Samples
Stirring and Temperature Control
Checklist for Best Results
53
Saving your Samples
Saving a Selected Spectrum
Retrieving Spectra
Deleting Current Spectra
Agilent 8453 UV-visible Spectroscopy System Operator's Manual
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