Uncalibrated Calculation Procedures; Area% And Height - Agilent Technologies 8453 Operator's Manual

5
Quantification

Uncalibrated Calculation Procedures

Uncalibrated Calculation Procedures

Uncalibrated calculation procedures do not require a calibration table.

Area% and Height%

The Area% calculation procedure reports the area of each peak in the run as a
percentage of the total area of all peaks in the run. Area% does not require
prior calibration and does not depend upon the amount of sample injected
within the limits of the detector. No response factors are used. If all
components respond equally in the detector and are eluted, then Area%
provides a suitable approximation of the relative amounts of components.
Area% is used routinely where qualitative results are of interest and to
produce information to create the calibration table required for other
calibration procedures.
The Height% calculation procedure reports the height of each peak in the run
as a percentage of the total height of all peaks in the run.
The multiplier and dilution factor from the Calibration Settings, from the Sample
Information dialog box, or from the Sequence Table are not applied in Area% or
Height% calculation.
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