Manual Integration - Agilent Technologies 8453 Operator's Manual

4
Integration

Manual Integration

Manual Integration

Manual Integration
This type of integration allows you to integrate selected peaks or groups of
peaks. Except for the initial area reject value, the software's event integration
is ignored within the specified range of manual integration. If one or more of
the peaks resulting from manual integration is below the area reject threshold,
it is discarded. The manual integration events use absolute time values. They
do not adjust for signal drift.
Manual Integration enables you to define the peak start and stop points, and
then include the recalculated areas in quantification and reporting.
Manually-integrated peaks are labeled in reports with the peak separation
code M. It offers the following features:
Draw Baseline Draw Baseline specifies where the baselines are to be drawn for a peak or set of
peaks. You can also specify whether peaks in the range given should be
automatically separated at all valley points.
Negative Peaks Negative Peaks specifies when to treat any areas below the baseline as negative
peaks. You can also specify whether peaks in the range given should be
automatically separated at all valley points.
Tangent Skim Tangent Skim calculates the areas of peaks tangentially skimmed off a main
peak. The area of the tangent skimmed peak is subtracted from the area of the
main peak.
Split Peak Split Peak specifies a point where to split a peak with a drop-line.
Delete Peak(s) Delete Peak(s) deletes one or more peaks from the integration results.
Peak Separation Codes for Manually-Integrated Peaks
Manually-integrated peaks are labeled in the integration reports by the peak
code MM.
If there is a peak before the manually-integrated peak, and the end of this peak
changes because of the manual integration, it is given the code F (forced).
A solvent peak which has been affected by manual integration, such as tangent
skim, are labeled R (re-calculated solvent).
110 Understanding Your Agilent ChemStation