Setting Up A Thermal Denaturation Method - Agilent Technologies 8453 Operator's Manual

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• The Stop column contains the final temperature for the ramp. When you

• The Increment column contains the value by which the temperature is

• The Hold Time column contains the time for which each temperature step is

Setting Up a Thermal Denaturation Method

In order to edit the analytical parameters of your thermal denaturation

method select Calculation in the method menu or press the setup button in the

analytical panel of your graphical user interface. This will open the

Calculation Parameters dialog box.

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add a ramp to the program, the stop temperature is undefined. The

maximum stop temperature is 100 °C.

increased step wise. The default increment is 0.5 °C.

maintained. The default hold time is 1.00 min.

Thermal Denaturation Mode

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