Agilent Technologies 8453 Operator's Manual page 88

4 Using your Agilent 8453 UV-visible Spectroscopy System
Quantitative Analysis using a Calibration with Standards
4 Setup your analysis wavelength at 273 nm (Use wavelength), type Caffeine
5 Click OK to set your parameters.
Now you are ready to run your measurements.
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for the Analyte name, set the Calibration curve type to Linear, select
Concentration entry and use mg/l as Unit. Check the Prompt for standard
information and the Prompt for sample information. Select Absorbance as
Data type and set Display spectrum From 190 nm To 340 nm.
Agilent 8453 UV-visible Spectroscopy System Operator's Manual