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Thermo Scientific Applied Biosystems QuantStudio Manuals
Manuals and User Guides for Thermo Scientific Applied Biosystems QuantStudio. We have
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Thermo Scientific Applied Biosystems QuantStudio manual available for free PDF download: User Manual
Thermo Scientific Applied Biosystems QuantStudio User Manual (126 pages)
3D Digital PCR System
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 8.71 MB
Table of Contents
Table of Contents
3
CHAPTER 1 Product Information
8
About the Quantstudio ™ 3D Digital PCR System
8
Instruments, Kits, Consumables, and Accessories
9
Required Materials Not Supplied
10
Power Line Regulator
12
Surge Protector
12
About the Instrument
12
Data Collection
13
Supported Dyes
13
About the Software
13
System Requirements
13
About the Chip
14
CHAPTER 2 Getting Started
15
Operational Workflow
16
Connect the Quantstudio ™ 3D Digital PCR Instrument to a Network
17
Software
17
Choosing a Thermal Cycler
17
Compatible Thermal Cyclers
17
CHAPTER 3 Prepare the Digital PCR Reaction and Load
19
The Chips
19
Guidelines for Loading and Sealing Chips
19
Prepare the DNA Samples
20
Quality of DNA
20
Quantity of DNA
20
Sample Dilution
21
Determine the Optimal Dilution When the Target Is Known
21
Determine the Optimal Dilution When the Target Is Unknown
22
Prepare the Digital PCR Reactions
22
Required Materials
22
Guidelines for Dpcr Sample Preparation
22
Recommended DNA Volume and Concentration
23
Prepare the Dpcr Reaction MIX for each Sample
23
Load the Chips Using the Quantstudio ™ 3D Digital PCR Chip Loader
24
Materials Required
24
Chip Loader Status Light
25
Prepare the Chip Loading Workspace
25
Prepare the Syringe Containing the Immersion Fluid
26
Load and Seal the Chips
27
Fill the Chip Case with Immersion Fluid
30
Seal the Chip Case with the Label
31
CHAPTER 4 Thermal Cycle the Chips
32
Materials Required
32
Prepare the Thermal Cycler
32
Guidelines for Handling Loaded Chips
35
Perform the PCR
36
Unload the Thermal Cycler
38
CHAPTER 5 Image and Analyze the Chips
40
About Imaging and Primary Analysis
40
Quantstudio 3D System Data Flow
40
About the Instrument Interface
41
Chip Imaging
42
Materials Required
42
Guidelines for Imaging Multiple Chips
42
Specify the Data Destination
42
Image the Chips
43
Primary Analysis
46
About Imaging Data and Primary Analysis
46
About the Results Screen
47
Data Quality Flags
48
About Data Files
48
Transfer the Results
49
Using the Quantstudio ™ 3D Analysissuite ™ Software
51
Access the Software from a Connect Cloud-Based Platform Account
51
Access the Software from the Server
51
Using the Quantstudio
51
Analysissuite
51
Obtain Information from the Help System
53
CHAPTER 6 Troubleshooting
54
Troubleshooting Chip Images Using the Chip View
54
APPENDIX A Install the Quantstudio
61
Digital PCR System
61
Time Required for Installation
61
Materials Required
61
Before You Begin
61
Plan the Laboratory Layout
62
Choose the Instrument Locations
62
Install the Quantstudio 3D Digital PCR Instrument
63
Install the Proflex ™ 2 × Flat PCR System
68
Unpack and Place the Proflex ™ PCR System Base Module
68
Prepare the Workspace and Instrument
69
Install the Tilt Base
69
Unpack and Install the Sample Block Module
71
Set up the Proflex ™ PCR System
71
Install the Geneamp ™ PCR System 9700
73
Unpack and Place the Geneamp ™ PCR System 9700 Base Module
73
Unpack and Install the Dual Flat Block Module
74
Install the Tilt Base and Chip Adapter
75
Set up the Geneamp ™ PCR System 9700
75
APPENDIX B Specifications and Layout
79
Component Dimensions and Weights
79
Quantstudio ™ 3D Digital PCR Instrument Layout and Connections
80
Instrument Clearances
80
Electrical Specifications
81
Environmental Requirements
82
Network Requirements
83
APPENDIX C Maintenance
84
Cleaning the Chip Tray and Sample Block
84
Materials Required
84
Clean the Chip Tray or Sample Block
85
Configure the Instrument Settings
86
(Optional) Set the Instrument Name
86
Set the Date and Time
87
Check the Instrument Firmware
87
Update the Instrument Firmware
88
Returning Your Instrument for Service
89
Replace the Instrument Fuses
90
Materials Required
90
Replace the Fuses
90
Calibrate the Touchscreen
91
View the Instrument Log
92
APPENDIX D Networking
94
Networking Overview
94
About the Network Port and Wireless Adaptor
94
Networking Guidelines and Best Practices
95
Connect the Instrument to the Network
95
Network Setup Workflow
96
Materials Required
96
Required Network Information
96
Connect the Quantstudio 3D Digital PCR Instrument to a Network
98
Create a Network Destination to Receive Run Data
103
APPENDIX E Load Chips Manually
106
Guidelines for Loading and Sealing Chips
106
Materials Required
107
Prepare the Syringe Containing the Immersion Fluid
107
Load and Seal the Chips Manually
108
Fill the Chip Case with Immersion Fluid
111
Seal the Chip Case with the Label
112
APPENDIX F Safety
113
Symbols on this Instrument
113
Conformity Symbols
114
Safety Alerts on this Instrument
115
Location of Safety Alerts on the Instrument
115
Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
116
Instrument Safety
116
General
116
Electrical Safety
117
Cleaning and Decontamination
118
Safety and Electromagnetic Compatibility (EMC) Standards
118
Safety
119
Emc
119
Environmental Design
119
Chemical Safety
120
Biological Hazard Safety
122
Documentation and Support
123
Related Documentation
123
Customer and Technical Support
123
Limited Product Warranty
124
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