Injecting Sample - Agilent Technologies 1200 Series Reference Manual

Injecting Sample

When using sample loops larger than 100 µl, mobile phase may be ejected from the
WA R N I N G
needle port as the mobile phase in the sample loop decompresses. Always use
appropriate safety procedures (for example, goggles, safety gloves and protective
clothing) as described in the material handling and safety data sheet supplied by the
solvent vendor, especially when toxic or hazardous solvents are used.
LOAD Position
In the LOAD position (see
column (ports 2 and 3 connected), and the needle port is connected to the
sample loop. At least 2 to 3 sample-loop volumes (more if better precision is
required) of sample should be injected through the needle port to provide
good precision. The sample fills the loop, and excess sample is expelled
through the vent tube connected to port 6.
Sample loop
Figure 12
1200 Series MI Reference Manual
Figure
LOAD Position
12), the pump is connected directly to the
Pump
Column
Needle port
Theory of Operation 4
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