Agilent Technologies 7500 ICP-MS Operator's Manual
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Agilent Technologies 7500 ICP-MS Operator's Manual

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Summary of Contents for Agilent Technologies 7500 ICP-MS

  • Page 1 Agilent 7500 ICP-MS ChemStation (G1834B) Operator’s Manual...
  • Page 3 Agilent Agilent Technologies shall not be liable software, firmware or hardware shall be Technologies office immediately. The for errors contained herein or for uninterrupted or error free.
  • Page 5 Agilent 7500 ICP-MS ChemStation(G1834B) Operator’s Manual G3270-90112...

  • Page 7: Table Of Contents

    Conventions Used in This Book P-5 Where to Go for More Information P-8 Agilent Technologies on Internet P-8 1 Using the Agilent 7500 ICP-MS ChemStation Software Accessing and Exiting the Software 1-2 Accessing the Software 1-2 Exiting the Software 1-5...
  • Page 8 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents 3 Startup, Shutdown and Status Starting the Instrument 3-3 Starting the Instrument from Cold 3-3 Starting from Shutdown Mode 3-7 Starting from Standby Mode 3-9 Shutting Down the Instrument 3-12 Putting the Instrument in Standby Mode 3-12...
  • Page 9 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents ShieldTorch System 4-18 Theory of the ShieldTorch System 4-18 At behind the interface 4-18 In the plasma 4-19 Feature of the ShieldTorch system 4-22 Effect of Carrier Gas Flow Rate 4-22 Each of the features for the three plasma conditions 4-25...
  • Page 10 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Each Parameter's Function 4-66 Information on Sensitivity Adjustment 4-70 Reducing Oxide Ions 4-84 Reducing Doubly Charged Ions 4-84 Scan of the Octopole 4-84 Tuning for Resolution and Mass Axis 4-87 Tuning the Detector 4-90...
  • Page 11 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Setting Method Filename 5-4 Selecting a Sample 5-6 Editing Element Information 5-7 Editing Report Information 5-10 Checking the Set Method 5-11 Using the Default Method as a Template 5-12 Editing the Default Method 5-13...
  • Page 12 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Editing QC Parameters 5-86 Saving a Method and Calibration 5-87 Modifying a Method 5-91 Loading a Method and Calibration 5-92 Modifying the Method Information 5-94 Modifying Data Acquisition Parameters 5-95 Printing a Summary of Method Acquisition Parameters 5-98...
  • Page 13 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Simulating a Chained Sequence 7-8 Viewing the Chained Sequence Log File 7-8 Saving the Chained Sequence 7-9 8 Running a Sample Analysis Running Samples by Using a Sequence 8-3 Loading a Sequence 8-4...
  • Page 14 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents 9 Viewing Spectra Displaying Spectra 9-3 Loading a Data File 9-4 Changing the Display Type 9-7 Using the Display Count Box 9-8 Changing the Vertical Scale of a Spectral Display 9-9 Changing the Horizontal Scale of a Spectral Display 9-10...
  • Page 15 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents 10 Viewing a Time Chart Displaying a Time Chart 10-3 Loading a Data File 10-4 Changing the Chart Type 10-8 Using the Display Count Box 10-11 Changing the Vertical Scale of a Time Chart 10-12...
  • Page 16 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Saving the Database as a Template 11-20 Updating a Custom Report Database 11-21 Viewing the Database 11-22 Creating Charts from a Custom Database 11-23 12 Performing a Quantitative Analysis Running Calibration Standards 12-3...
  • Page 17 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Viewing the Results of Semiquantitative Analysis 13-3 Loading a Data File 13-4 Entering Concentration Values 13-6 Entering Minimum Peak Value 13-7 Selecting Concentration Units for the Report 13-8 Entering and Deleting Counts Per Second (cps) Values 13-9...
  • Page 18 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents 15 Performing an Isotope Dilution Analysis Setting Isotopic Dilution Analysis Parameters 15-3 Generating an Isotope Dilution Analysis Report 15-9 16 Tools Menu DoList 16-2 How to Use DoList? 16-2 Configure DoList 16-5 How to Configure DoList? 16-5...
  • Page 19 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Printer Settings 18-21 Installing the FlashPlayer 18-25 Installing the Agilent 7500 ChemStation Software 18-26 Initialization of a Tune File to the Factory Default Condition 18-33 Installation the Patch Files 18-35 GPIB Configuration 18-36...
  • Page 20 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents Installation the Patch Files 19-35 GPIB Configuration 19-36 If No communication with 7500 when GPIB is 82350A or 82350B 19-38 20 Appendix Appendix A A-2 Top Task A-2 Instrument/Tuning Task A-4 Instrument Control A-4...
  • Page 21 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents DL A-21 Standard Deviation of the Concentration A-22 Interpolation Formulas for Virtual-Internal-Standard Correction A-23 SemiQuantitation A-26 Averaging Repetition Files A-28 Appendix C A-29 Dead Time Calibration A-29 Execution of Dead Time Calibration A-30...
  • Page 22 Agilent 7500 ICP-MS ChemStation Operator’s Manual Contents C-16...
  • Page 23 Preface...

  • Page 24: Preface

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Preface The Agilent 7500 ICP-MS ChemStation Operator's Manual explains the procedures necessary to use the Agilent 7500 for both sample and data analysis. It also provides information about accessing the software and ensuring optimal instrument performance.
  • Page 25 Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Chapter 5, “Creating a Method”, explains how to create a method for data acquisition. Chapter 6, “Setting Up a Sequence”, explains how to arrange samples when analyzing samples automatically. Chapter 7, “Chained Sequence”, explains how to edit and use chained sequence.
  • Page 26 Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Chapter 19, “Installing the Agilent 7500 ChemStation Software (Windows 2000)”, explains how to set up the computer and install the ChemStation software. Appendix A, provides a reference of Agilent 7500 ChemStation menus. You can quickly determine which menu to access for a specific purpose.

  • Page 27: Conventions Used In This Book

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Conventions Used in This Book This book uses certain conventions to distinguish different types of information easily. This section describes these conventions. Instructions All step-by-step instructions are numbered and in bold, as in the following example.
  • Page 28 Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Terminology This book frequently uses the following terms: Convention Information Press To hold down a button on the keyboard. Click To press and release the mouse button. Double-click To click the mouse button twice in rapid succession.
  • Page 29 Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Notes Notes contain a reminder about the effect of particular actions. They are indicated as follows: N O T E This example shows how a note is displayed. Cautions Cautions indicate situations that could overwrite data or require immediate attention to prevent harm to the instrument.

  • Page 30: Where To Go For More Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Preface Where to Go for More Information In addition to the Agilent 7500 ICP-MS ChemStation Operator's Manual, you can reference the following resources: • Agilent 7500 ICP-MS Hardware Manual • Agilent 7500 ICP-MS Option Instruction Manual •...
  • Page 31 Using the Agilent 7500 ICP-MS ChemStation Software...

  • Page 32: Using The Agilent 7500 Icp-Ms Chemstation Software

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software Using the Agilent 7500 ICP-MS ChemStation Software The Agilent 7500 inductively coupled plasma-mass spectrometer (ICP-MS) is capable of measuring trace elements at levels as low as one part per trillion or quickly scanning more than 70 elements to determine an unknown sample's composition.
  • Page 33 Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software The ICP-MS Top icon starts the software and allows you to access ChemStation functions pertaining to the instrument, data acquisition, and data analysis. It also enables you to create and run methods and sequences.
  • Page 34 Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software The ICP-MS Top window appears when the ICP-MS Top is selected The ICP-MS Top window shows the last window displayed when ChemStation was closed. To use a function of ChemStation, select it on the menu bar or click on the icon.

  • Page 35: Exiting The Software

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software Exiting the Software Use any one of the following methods to exit ChemStation. Be sure to save any changes before exiting. • Double-click the Control Box Menu in the upper left corner of the ICP-MS Top window, or click the Control Box Menu and then click Close in the pop-up menu that appears.

  • Page 36: Moving Within The Software

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software Moving within the Software The ChemStation Top window remains open while you use ChemStation. To open other ChemStation windows, select items from the Top drop down menus or the tool bar.

  • Page 37: For More Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software For More Information For more information on using the mouse and on other Windows features, refer to your Microsoft Windows documentation and the online Windows tutorial. Using the Command Line The command line is used to create and edit macros to customize ChemStation.

  • Page 38: Supplemental Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Using the Agilent 7500 ICP-MS ChemStation Software Supplemental Information Listed in this section is useful information for using the ICP-MS ChemStation software: • File names should not be any longer than 8 letters and/or numbers.
  • Page 39 Configuration...

  • Page 40: Configuration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration Configuration If you have changed the system configuration, change the software settings accordingly. When you start the Configuration task, the ICP-MS Configuration dialog box appears, enter sample introduction settings such as whether to use a peristaltic pump, autosampler, etc.
  • Page 41 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration ICP-MS Configuration Dialog Box 2 Set the Offline Instrument check box to OFF (do not check this box). On-line is the state in which data is transferred via the LAN or GPIB interface. In...
  • Page 42 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration Select this if you do not want to use the remote start mode. • Wait until Ready Signal Select this if you want to use the remote start mode. 5 Set the sample introduction.
  • Page 43 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration N O T E Only one LC/GC ChemStation can synchronize with the ICP-MS ChemStation, and you can control only a single instrument from the LC/GC ChemStation. C A U T I O N To use the LC/GC ChemStation, you must enable Remote Start.
  • Page 44 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration • COM Port for ALS Specify the COM port to which you want the Autosampler connected. Normally this is COM1. • Racks (ASX500 or ASX100) For the ASX500, the racks are, from left to right, identified as Rack ID 1, Rack ID 2, Rack ID 3, and Rack ID 4.
  • Page 45 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration 7 Check EM Protection Normally, leave Auto setting of integ time in analog mode selected. If the detector mode is set to auto, the dwell time in analog mode is fixed at 100 microseconds so that large signals do not damage the detector, regardless of the dwell time value specified in the method.
  • Page 46 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration 9 Match the ChemStation configuration with the Instrument Hardware. The Miscellaneous dialog box appears when the Miscellaneous button is clicked. Miscellaneous Dialog Box • Hardware Option SC Cooling: In normal operation when introducing a liquid sample, the spray chamber is cooled to stabilize the fine particles supplied to the plasma.
  • Page 47 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration ISIS External Pump: To enable this checkbox, select ISIS for Type in Sample Introduction in the ICP-MS Configuration dialog box, then select Discrete Sampling as the ISIS Application. Select this checkbox to use ISIS with an external pump. Selecting the checkbox displays the following dialog box.
  • Page 48 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration • Remote Shutdown Standard (Default) The Standard option must be selected when the ICP-MS is connected to the ISIS. Agilent LC The Agilent LC option must be selected when ISIS is used with an external pump.
  • Page 49 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration N O T E In normal use, there is no need to select Use Dead Time calibration. For more information, see Appendix C, “Dead Time Calibration”. Click OK when you complete the detail setting.
  • Page 50 Agilent 7500 ICP-MS ChemStation Operator’s Manual Configuration N O T E The Reset to Default, Save, Cancel, and Help buttons are displayed in the ICP-MS Configuration dialog box. If Reset to Default is clicked, it restores the factory settings (Except the setting of the toolbar and the QC configuration). If Cancel is clicked, it cancels the change of setting, and ends the ICP-MS Configuration dialog box.
  • Page 51 Startup, Shutdown and Status...

  • Page 52: Startup, Shutdown And Status

    Startup, Shutdown and Status Startup, Shutdown and Status The Agilent Technologies, Agilent 7500 is designed so that it is easy to start up and shut down. The instrument has three states of operation that are displayed in the Agilent 7500 ChemStation instrument control software: •...

  • Page 53: Starting The Instrument

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Starting the Instrument There are three states from which the Agilent 7500 can be started: • Unplugged • Shutdown mode • Standby mode This section explains how to start the instrument from all three states.
  • Page 54 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Power Switch for the Rotary Pumps Main Power Breaker Main Power Supply Cable Power Connection for the Rotary Pumps Rear View and Control Switches 5 Turn on the instrument power switch.
  • Page 55 Refer to the printer and computer user manuals for more information. 7 Start the ChemStation software. Refer to Chapter 1, “Using the Agilent 7500 ICP-MS ChemStation Software” for information on exiting the software. The ICP-MS Top window appears. The ICP-MS Top Window...
  • Page 56 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Instrument Control Window in Shutdown Mode (Picture may differ depending on ICP-MS Model/Mode)

  • Page 57: Starting From Shutdown Mode

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Starting from Shutdown Mode The following instructions assume that the instrument is turned on and the Instrument Control window is displayed. See “Starting the Instrument from Cold” to perform those tasks. Then, complete the following steps: 1 Select Vacuum>>Vacuum On.
  • Page 58 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Instrument Control Window in Standby Mode (Picture may differ depending on ICP-MS Model/Mode)

  • Page 59: Starting From Standby Mode

    100 ± 2.8 psi (700 ± 20 kPa) 5 Ensure that the drain vessel is empty. See Chapter 2, “Precautions” in the “Agilent 7500 ICP-MS Hardware Manual”, for information on emptying the drain vessel. 6 Ensure that the gas tubing is not pinched and that the torch box and sample introduction gas connections seal correctly.
  • Page 60 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status C A U T I O N Be sure clamp the peripump tubing for the drain in the peripump. The drain from the spray chamber is provided by this peripump. If the spray chamber is not drained properly, it will fill with solution causing solution to flow to the torch which will cause the plasma to turn off.
  • Page 61 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Instrument Control Screen in Analysis Mode (Picture may differ depending on ICP-MS Model/Mode) WAR N I N G Flow direction (Drain Vessel) (PeriPump) Waste Solution Confirm that the drain is flowing. Check that the tube from the drain peristaltic pump to the drain bottle is properly connected.

  • Page 62: Shutting Down The Instrument

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Shutting Down the Instrument The Agilent 7500 is in Analysis mode during tuning and sample analysis. When not being used for these procedures, the instrument remains in Standby mode. To maintain or move the instrument, it should be placed in Shutdown mode and turned off.

  • Page 63: Putting The Instrument In Shutdown Mode

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status N O T E While the instrument is in the Standby mode, turning off the computer and the printer will not cause any problems. Putting the Instrument in Shutdown Mode...

  • Page 64: Turning The Instrument Completely Off

    Follow the instructions in “Putting the Instrument in Shutdown Mode” 2 Exit the software and turn off the computer and printer Refer to Chapter 1, “Using the Agilent 7500 ICP-MS ChemStation Software”, and the printer and computer user’s manuals for more information.

  • Page 65: Checking The Instrument Status

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Checking the Instrument Status The Agilent 7500 ChemStation software (ChemStation) provides two methods for checking the instrument status: • Instrument Control Diagram • Meter Control Panel This section explains how to use these monitoring screens.

  • Page 66: Checking The Meter Control Panel

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status 3 Moving the cursor clockwise, place it on the next gauge and press the left mouse button when the cursor changes to the small meter box. The Carrier Gas Flow meter box appears. An acceptable range of values for this meter is application specific.
  • Page 67 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status N O T E Meter boxes are displayed until you close them individually or close the Instrument Control window. Meter boxes that are open when the Instrument Control window is...
  • Page 68 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Meter Control Panel Values M e te r T ypi cal Ra n g e Shut D ow n M ode St andb y M ode A n aly s is M ode...

  • Page 69: Ion Lenses Test

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status (continued) Meter Typical Range Unit Shut Down Mode Standby Mode Analysis Mode Carrier Gas Flow 0.8 to 1.3 L/min Makeup Gas Flow 0 to 1.0 L/min Optional Gas Flow Depends on...

  • Page 70: Nebulizer Test

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status The Ion Lenses Test dialog box will be displayed. Ion Lenses Test dialog box The ion lenses will be checked and the measured values displayed in the Inputs area five seconds later.
  • Page 71 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status The Nebulizer Test dialog box will be displayed. Nebulizer Test dialog box 3 Select the type of nebulizer from the Nebulizer Type area. 4 Adjust the source pressure of the argon gas to fit within the appropriate range using a regulator.
  • Page 72 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status The following message appears when the source pressure is within the appropriate range. This dialog box appears when the source pressure is within the appropriate range Click Yes to execute the nebulizer test.
  • Page 73 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status If the average value of the source pressures of the argon gas is 100 kPa or less, a message indicating that the test has failed will be displayed in the Current Situation area.

  • Page 74: Typical Meter Values For The Vacuum System

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status Typical Meter Values for the Vacuum System Refer to the following meter values when the error occurs. 1 After the vacuum is ON Parameters Limit Conditions Backing pressure (BK) > 100 Pa if BK is higher, even 60 seconds after the vacuum is ON.
  • Page 75 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status 4 In Standby mode Parameters Limit Conditions Analyzer pressure (AN) > 2 x 10 Agilent 7500 goes back to Shutdown mode. Backing pressure (BK) > 40 Pa Agilent 7500 goes back to Shutdown mode.
  • Page 76 Agilent 7500 ICP-MS ChemStation Operator’s Manual Startup, Shutdown and Status 3-26...
  • Page 77 Tuning...

  • Page 78: Tuning

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning Tuning allows you to monitor and adjust Agilent 7500 performance before beginning sample analysis. Tuning and sample analysis should be performed in analysis mode. You can change tuning parameters to adjust the instrument’s condition for a particular application.

  • Page 79: Overview

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Overview Tuning involves the following steps: • Introducing and ionizing samples efficiently in the plasma and introducing the ions into the vacuum systems See “Tuning for Sensitivity (Agilent 7500a)” on page 4-4. It is especially important to set the plasma condition for reducing the oxide and/or doubly Charged Ions.

  • Page 80: Tuning For Sensitivity (Agilent 7500A)

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning for Sensitivity (Agilent 7500a) Tune the Agilent 7500 for sensitivity to ensure that the instrument produces the best results for the masses being analyzed. Achieve good sensitivity by running a recommended tuning solution of 10 parts per billion (ppb) of Li, Y, Ce and Tl.
  • Page 81 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning >> 2 Select Tune Acq. Params. The Acquisition Parameters - Sensitivity dialog box appears. Use this dialog box to set or change the masses you want to view, the integration time, and the ratio of any two channels. You can also display the ratio of signal strengths of any two selected masses.
  • Page 82 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 7 Select the Plot check boxes of the channels to be displayed on the graph in the ICP-MS Tuning - Sensitivity window. 8 In the Displayed Tuning Parameters tree display, select the check boxes of the tuning parameters to be displayed in the ICP-MS Tuning - Sensitivity window.
  • Page 83 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Clicking on a parameter will cause a text box or a scroll bar to appear at the lower center section of the window. Use one of the following methods to set a parameter value.

  • Page 84: Plasma Condition

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical value of Sensitivity and RSD (Using the normal torch) Counts / 10 ppb Mass Integ. Time = 0.1sec >6400 <15% >16000 <15% >9600 <15% Plasma Condition • RF Power Controls RF power supplied to the load coil. A higher RF power increases the sensitivity and reduces oxide and doubly charged ions.
  • Page 85 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning adjustment of Torch-H/V will be required. If these parameters are changed using the software scroll bar, the plasma may turn off. Use only the scroll arrows. • Carrier Gas Controls nebulization efficiency of sample and sample uptake rate when self-aspiration is applied.

  • Page 86: Ion Lenses

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Ion lenses • Extraction 1 & 2 It extracts ions from the plasma to accelerate them toward the Einzel lens. Adjust to get enough sensitivity for all masses. This is lens that needs the most frequently adjusted everyday.
  • Page 87 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning • QP Bias Controls the speed of ions as they pass through the Q-pole. A typical value is 0 V without the ShieldTorch system. It may be used up to 6 V when the peak shape and resolution are not good.

  • Page 88: Reducing Oxide Ions

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Reducing Oxide Ions When changing tuning parameters to achieve greater sensitivity, you can inadvertently increase the level of oxide ions. The Sensitivity tuning window can be used to check for oxide ions, but ChemStation provides another window with the mass values already set for the standard tuning solution, 10 parts per billion (ppb) of Li, Y, Ce and Tl.
  • Page 89 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 3 Select Tune >> Acq. Params. The Acquisition Parameters - Oxide Ion dialog box will appear. Acquisition Parameters - Oxide Ion Dialog Box 4 Select the check boxes of the channels to be displayed in the ICP-MS Tuning - Oxide Ion window.
  • Page 90 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Parameters can be displayed or hidden by clicking on the [+]/[-] box. Clicking on a parameter check box will alternately select or deselect the parameter. A parameter in gray indicates that only some of the parameters contained within are selected.

  • Page 91: Reducing Doubly Charged Ions

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Reducing Doubly Charged Ions When tuning parameters are changed to achieve greater sensitivity, this can inadvertently increase the level of doubly charged ions. You can use the Sensitivity tuning window to check for doubly charged ions, but ChemStation provides another window with the mass values already set for the standard tuning solution.
  • Page 92 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 3 Select Tune >> Acq. Params. The Acquisition Parameters - Doubly Charged Ion dialog box will appear. Acquisition Parameters - Doubly Charged Ion Dialog Box 4 Select the check boxes of the channels to be displayed in the ICP-MS Tuning - Doubly Charged Ion window.
  • Page 93 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 8 In the Displayed Tuning Parameters tree display, select the check boxes of the tuning parameters to be displayed in the ICP-MS Tuning - Doubly Charged Ion window. Parameters can be displayed or hidden by clicking on the [+]/[-] box.

  • Page 94: Shieldtorch System

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning ShieldTorch System The ShieldTorch system is very powerful with features such as a Cool plasma for Fe, K, Ca analysis at the ppt level, a High sensitivity mode and a Soft extraction mode.

  • Page 95: In The Plasma

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Work coil potential Plasma potential Ion energy Matching RF source Network Sampling Cone Skimmer Torch Cone Sample In Work Coil Electrical Model of the Plasma and the Interface Region In the plasma The ionization efficiency of elements in the Ar plasma can be calculated using the...
  • Page 96 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning × If the electron density is 1.47 and the electron temperature is 6,680K, the ionization efficiency of elements will be as shown in the next figure. Elements that have lower ionization potentials such as Li, Na, and Lanthanoids can be ionized with nearly 100 % efficiency in the plasma.
  • Page 97 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning potentials of polyatomic ions are relatively high, probably higher than 10 eV and similar to that of Cl. 0.01 0.001 0.0001 0.00001 0.000001 0.0000001 4,500 5,000 5,500 6,000 6,500 7,000 7,500 Electron Temperature (K)

  • Page 98: Feature Of The Shieldtorch System

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Feature of the ShieldTorch system There is a clear difference between the standard system (without the ShieldTorch) and the ShieldTorch system. Each has advantages and disadvantages, therefore it must be considered which system is better for the samples and elements to be analyzed.
  • Page 99 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning rates, while Ba is very low. As an example, the relationship between carrier gas flow rate and signal using the elements in the table is shown in the next figure. Chemical / Physical Characterisisties of Selected Elements...
  • Page 100 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Carrier Gas Flow Carrier Gas Flow Li, Be Co, In, Pb Co, As Be, Ba In, Ba, W, Pb As, W Carrier Gas Flow Carrier Gas Flow BaOH Ba2+ BaOH Ba2+ Carrier Gas Flow...

  • Page 101: Each Of The Features For The Three Plasma Conditions

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Each of the features for the three plasma conditions There are 3 combinations to be considered: the standard system with normal plasma conditions, the ShieldTorch system with normal plasma conditions, and the ShieldTorch system with cool plasma conditions. Some features of each follow:...
  • Page 102 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning The ShieldTorch system (Cool plasma) Advantage • Analysis of elements which suffer interference from polyatomic ions due to Ar and C. Ar --- Ca (Ar), K (ArH), Cr (ArC), and Fe (ArO) C --- Mg (C ), Cr (ArC) •...

  • Page 103: Notes For The Use Of The Shieldtorch System

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Notes for the use of the ShieldTorch system Plasma ignition • Igniting the plasma is a little more difficult compared to that of the standard torch because the Shield plate is between the torch and the coil.

  • Page 104: Tuning Solution

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning solution Mg, Y, Ce, and Tl, solution at 1 ppb N O T E For the high sensitivity mode, Li is not used because it is difficult to reduce the background. Li remains on the interface as memory.
  • Page 105 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values for the ShieldTorch (High Sensitivity mode Tuning parameters Typical Range Note ∼ RF Power (W) 1500 1200 1600 ∼ Smpl Depth (mm) Set lower than the normal value. ∼ Carrier Gas (L/min) ∼...
  • Page 106 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 8 Adjust the Carrier Gas and Makeup Gas flow rate. 9 Adjust the PeriPump (sample flow rate). 10 Adjust lens parameters. 1) Adjust the extraction lenses. Adjust the Extract 1 in 10V increments until reaching a plateau sensitivity, and then adjust the Extract 2 in 1V increments until reaching a plateau sensitivity.

  • Page 107: Tuning The Shieldtorch (Soft Extraction Mode)

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning the ShieldTorch (Soft Extraction Mode) The Soft Extraction mode gives the lowest background. N O T E The Agilent 7500 model is constructed prestigiously with two rotary pumps. The two rotary pumps give the best performance with the highest sensitivity. To achieve maximum performance of the two rotary pumps, make sure the valve on the top of the second rotary pump is open.
  • Page 108 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values for the ShieldTorch (Soft extraction mode) Tuning parameter Typical Range Note ∼ RF Power (W) 1500 In the Soft extraction mode, a higher plasma temperature setting 1200 1600 is necessary. The Carrier gas flow rate and Sampling depth also affect the plasma temperature.
  • Page 109 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 4 Adjust the torch position (Torch-H and Torch-V). 5 Adjust the flow rates of the Carrier gas and the Makeup gas. 6 Adjust the Peristaltic pump speed (Sample flow rate). 7 Adjust the RF power.
  • Page 110 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Element Count/ 1 ppb(Integ.: 0.1 sec) Back ground (cps, off mass)* ∼ ∼ Li (7 amu) ∼ Y (89 amu) >30,000 ∼ Tl (205 amu) >20,000 *Shown on the tuning report. N O T E If the background is compromised, it is easy to increase low mass sensitivity to more than100 MCPS/ppm.

  • Page 111: Tuning The Shieldtorch (Cool Plasma)

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning the ShieldTorch (Cool plasma) Ca (40 AMU) can be analyzed with the cool plasma conditions not the normal plasma conditions. To do that, delete the 40 AMU in the Prohibited Masses of the Configuration task.
  • Page 112 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values for the ShieldTorch (Cool plasma) Tuning parameter Typical Range Note ∼ RF Power (W) Adjust the RF power lower than the standard torch.While 1000 monitoring the RF reflection power from the Tuning window, make the RF power lower from the normal plasma (1200-1400 W) to 900 W.
  • Page 113 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning parameter Typical Range Note ∼ QP Focus (V) Because the ion energy is low, it is necessary to make this value smaller than the standard torch. Make sure to use with 0 or (+).
  • Page 114 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning sensitivity increasing carrier gas flow Behavior of Co, ArH and ArO 10 Check the background level If the tuning solution contains impurities and it is difficult to check the background of 39amu(ArH) or 56amu(ArO). Use fresh ultra pure water and check if the signal is decreased.

  • Page 115: Tuning For Sensitivity (Agilent 7500Ce)

    C A U T I O N Whenever the Agilent 7500cs is used, the ShieldTorch must be installed in it. For installation of the ShieldTorch, refer to the Agilent 7500 ICP-MS Hardware Manual. Adjust sensitivity using the following steps: 1 Select Instrument>>Tune in the ICP-MS Top Window.
  • Page 116 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 2 Select Tune>>Acq. Params. The Acquisition Parameters-Sensitivity Dialog Box will appear. Use this dialog box to set or change the masses you want to view, the integration time, or the ratio of any two channels.
  • Page 117 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 7 Select the Plot check boxes of the channels to be displayed on the graph in the ICP-MS Tuning - Sensitivity window. 8 In the Displayed Tuning Parameters tree display, select the check boxes of the tuning parameters to be displayed in the ICP-MS Tuning - Sensitivity window.
  • Page 118 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Clicking on a parameter will cause a text box or a scroll bar to appear at the lower center section of the window. Use one of the following methods to set a parameter value.

  • Page 119: Each Parameter's Function

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Each Parameter's Function The working of each parameter used for sensitivity adjustment is described below. N O T E The Agilent 7500ce has three modes: Standard Mode, which does not use a reaction...
  • Page 120 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning • Makeup Gas Makes up Ar gas into the spray chamber and is mixed with the carrier gas. When the Ar gas supply pressure is not high enough to get a sufficient carrier gas flow, the makeup gas is used.
  • Page 121 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning • Extract 2 It focuses the ion beam from the extraction lens. An element of low mass gets maximum sensitivity at low voltage. On the other hand, an element of high mass gets maximum sensitivity at high voltage. Therefore, set a value to get optimum sensitivity foe the whole mass range.

  • Page 122: Information On Sensitivity Adjustment

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning resolution are not good, adjust it up to -2 V. QP bias needs to be more positive than the OctP bias by the value shown as below: • In standard mode: 2 to 4 V (Block polyatomic ions produced from the residual gas in the ion lens chamber.)
  • Page 123 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning of the lens voltage. Adjustment of the reaction gas flow rate is done by measuring the flow rate correlation of the BEC (background equivalent concentration) with a blank solution and a standard solution. This is described below.
  • Page 124 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values of Tuning Parameters in the Standard Mode (with ShieldTorch, Micro Mist Nebulizer) Parameter Typical Value Adjustment RF Power (W) 1500 Normally used 1500 Sampling Depth (mm) 7 to 10 Carrier Gas (L/min) Normally used 0.9...
  • Page 125 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Mode (When Hydrogen is used as Reaction Gas) It is recommended to tune in H mode after tuning in standard mode. Autotune cannot be used in H mode The following is an example of the sensitivity tuning method in the H mode.
  • Page 126 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning h.(Background Check) Aspirate fresh DI water and measure the background of mass (56 amu and 78 amu), which is to be measured in H mode. If the count of 56 amu and 78amu is too high, increase 0.4 mL/min of H flow rate, and then repeat step e to step g.
  • Page 127 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning The higher the flow of H gas, the lower the intensity of the sample and the background. Adjust the flow in order to make the background low enough and to get a necessary level of sensitivity. To optimize the flow rate of H...
  • Page 128 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 7) Display the signals by clicking the Check Count button and confirm that the signal is stable. At this time, the flow rate of the H gas doesn't change. Click the Stop button after confirming signal stability.
  • Page 129 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Reaction Gas Graph Dialog Box N O T E The Reaction Gas Report will appear when you click the Tabulate button in the ICP-MS Tuning-Reaction Gas Window. 15)Click the Close button, close the Reaction Gas Graph Dialog Box, and return to the ICP-MS Tuning-Reaction Gas Window.
  • Page 130 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning N O T E Once the parameter of the reaction gas flow rate is tuned; only a fine adjustment of the flow rate is enough for daily tuning. He Mode (When Helium is used as the Reaction Gas) It is recommended to tune in He mode after tuning in H mode.
  • Page 131 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values of Tuning Parameters in the He Mode (with ShieldTorch, Micro Mist Nebulizer) Parameter Typical Value Adjustment RF Power (W) 1500 Normally used 1500 Sampling Depth (mm) 7 to 10 Carrier Gas (L/min) Normally used 0.9...
  • Page 132 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 1) Select Tune>>Reaction Gas in the ICP-MS Tuning Window. The ICP-MS Tuning-Reaction Gas Window will appear. In this window, the data of the tuning and blank solutions are collected as the gas flow rates change. The Graph button will produce a graph of the sensitivity and BEC (Background Equivalent Concentration).
  • Page 133 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 8) Click the Ramp Flow button. Sensitivity data is collected by changing the He gas within the flow rate range that was previously set. A message will be displayed on the message line after the data collection is complete.
  • Page 134 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Reaction Gas Graph Dialog box N O T E The Reaction Gas Report will appear when you click the Tabulate button in the ICP-MS Tuning-Reaction Gas Window. 15)Click the Close button, close the Reaction Gas Graph Dialog Box, and return to the ICP-MS Tuning-Reaction Gas Window.

  • Page 135: Reducing Oxide Ions

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning N O T E It is good practice for the tuning parameter to be saved under a different file name from a standard mode or H mode tune file. N O T E Once the parameter of the reaction gas flow rate is tuned;...
  • Page 136 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning RF Voltage OctP RF (Tuning parameter) Ramp Offset QP Mass Bend Mass Scan line of Octopole Three parameters are used to determine this scan line: • Ramp Offset: The Offset voltage of the RF voltage in the mass portion area.
  • Page 137 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Octopole Setup Dialog Box 3 Set each parameter necessary to scan the Octopole • Enable Scan: To scan, input a check mark in this check box. If there is no check mark in the check box, Octopole will not be scanned. Usually, leave the check box blank.

  • Page 138: Tuning For Sensitivity (Agilent 7500Cs)

    C A U T I O N Whenever the Agilent 7500cs is used, the ShieldTorch must be installed in it. For installation of the ShieldTorch, refer to the Agilent 7500 ICP-MS Hardware Manual. Adjust sensitivity using the following steps: 1 Select Instrument>>Tune in the ICP-MS Top Window.
  • Page 139 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 2 Select Tune>>Acq. Params. The Acquisition Parameters-Sensitivity Dialog Box will appear. Use this dialog box to set or change the masses you want to view, the integration time, or the ratio of any two channels. You can also display the ratio of signal strengths of any two selected masses.
  • Page 140 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 7 Select the Plot check boxes of the channels to be displayed on the graph in the ICP-MS Tuning - Sensitivity window. 8 In the Displayed Tuning Parameters tree display, select the check boxes of the tuning parameters to be displayed in the ICP-MS Tuning - Sensitivity window.
  • Page 141 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Clicking on a parameter will cause a text box or a scroll bar to appear at the lower center section of the window. Use one of the following methods to set a parameter value.

  • Page 142: Each Parameter's Function

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Each Parameter's Function The working of each parameter used for sensitivity adjustment is described below. N O T E The Agilent 7500cs has four modes: Standard Mode, which does not use a reaction...
  • Page 143 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning a gas flow of 0.8 L/min. • Makeup Gas Makes up Ar gas into the spray chamber and is mixed with the carrier gas. When the Ar gas supply pressure is not high enough to get a sufficient carrier gas flow, the makeup gas is used.
  • Page 144 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning may be high in reaction mode (H mode or He mode) • Extract 2 It focuses the ion beam from the extraction lens. An element of low mass gets maximum sensitivity at low voltage. On the other hand, an element of high mass gets maximum sensitivity at high voltage.
  • Page 145 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning • QP Bias It controls the speed of ions when an element ion passes through the Q-Pole and prevents polyatomic ions of low energy from passing. When peak shape and resolution are not good, adjust it up to -2 V.

  • Page 146: Information On Sensitivity Adjustment

    It is good practice for tuning parameter to be saved under different file names after tuning in each mode. N O T E For the ShieldTorch and Cool Plasma, refer also to the "Agilent 7500 ICP-MS Option Instruction Manual". Standard Mode (When Reaction Gas is Not Used) Tune according to the function of each parameter above (Page 4-66) and the table of each parameter's value.
  • Page 147 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values of Tuning Parameters in the Standard Mode (with ShieldTorch, Micro Flow Nebulizer, self-aspiration) Parameter Typical Value Adjustment RF Power (W) 1500 Normally used 1500 Sampling Depth (mm) 7 to 10 Carrier Gas (L/min) 0.6 to 1.0...
  • Page 148 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Mode (When Hydrogen is used as Reaction Gas) It is recommended to tune in H mode after tuning in standard mode. Autotune cannot be used in H mode The following is an example of the sensitivity tuning method in the H mode.
  • Page 149 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning i. If the sensitivity is low, change the Omega Bias-cs and the Cell Exit to a lower voltage. In this case, sometimes, the background may increase. Monitor the background when adjusting. Typical values of Tuning Parameters in the H...
  • Page 150 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 2 Adjustment of H gas flow The higher the flow of H gas, the lower the intensity of the sample and the background. Adjust the flow in order to make the background low enough and to get a necessary level of sensitivity.
  • Page 151 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 7) Display the signals by clicking the Check Count button and confirm that the signal is stable. At this time, the flow rate of the H gas doesn't change. Click the Stop button after confirming signal stability.
  • Page 152 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Reaction Gas Graph Dialog Box N O T E The Reaction Gas Report will appear when you click the Tabulate button in the ICP-MS Tuning-Reaction Gas Window. 15)Click the Close button, close the Reaction Gas Graph Dialog Box, and return to the ICP-MS Tuning-Reaction Gas Window.
  • Page 153 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning N O T E Once the parameter of the reaction gas flow rate is tuned; only a fine adjustment of the flow rate is enough for daily tuning. He Mode (When Helium is used as the Reaction Gas) It is recommended to tune in He mode after tuning in standard mode.
  • Page 154 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning g.Readjust the Omega Lens-cs, QP Focus, and OctP RF to obtain best sensitivity. h.(Background Check) Aspirate fresh DI water and measure the background of mass (58 amu), which is to be measured in He mode. If the count of 58 amu is too high, increase 0.4 mL/min of He flow rate, and then repeat step e...
  • Page 155 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values of Sensitivity (He Mode) Mass Count/1 ppb Integration time = 0.1 sec. > 3000 < 15% 2 Adjustment of He gas flow rate The higher the flow of He gas, the lower the intensity of the sample and background.
  • Page 156 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 4) Input the concentration of each mass number of the tuning solution in the Std Conc Text Box and select the concentration unit in Conc Unit. 5) Select Std (standard solution) in Current Solution.
  • Page 157 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Reaction Gas Graph Dialog box N O T E The Reaction Gas Report will appear when you click the Tabulate button in the ICP-MS Tuning-Reaction Gas Window. 15)Click the Close button, close the Reaction Gas Graph Dialog Box, and return to the ICP-MS Tuning-Reaction Gas Window.
  • Page 158 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning N O T E It is good practice for the tuning parameter to be saved under a different file name from a standard mode or H mode tune file. N O T E Once the parameter of the reaction gas flow rate is tuned;...
  • Page 159 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Typical values of Tuning Parameters in the Cool Mode (with ShieldTorch, Micro Flow Nebulizer, self-aspiration) Parameter Typical Value Adjustment RF Power (W) Normally use 600 Sampling Depth (mm) Normally use 18 Carrier Gas (L/min) Normally use 0.7...

  • Page 160: Reducing Oxide Ions

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning N O T E It is recommended that the 2 tuning items, "EM" and "Resolution / Axis", are executed in standard mode. Autotune is executed with higher sensitivity in standard mode. In cool mode, execute Autotune without "EM" and "Resolution / Axis".
  • Page 161 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning RF Voltage OctP RF (Tuning parameter) Ramp Offset QP Mass Bend Mass Scan line of Octopole Three parameters are used to determine this scan line: • Ramp Offset: The Offset voltage of the RF voltage in the mass portion area.
  • Page 162 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Octopole Setup Dialog Box 3 Set each parameter necessary to scan the Octopole • Enable Scan: To scan, input a check mark in this check box. If there is no check mark in the check box, Octopole will not be scanned. Usually, leave the check box blank.

  • Page 163: Tuning For Resolution And Mass Axis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning for Resolution and Mass Axis Resolution and mass axis tuning is important because when the Agilent 7500 acquires data, it will scan each mass according to the tuning parameters. To tune the resolution and mass axis, you use the recommended tuning solution of 10 ppb of Li, Y, and Tl to ensure acceptable responses over a wide range of masses.
  • Page 164 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning The window includes three boxes. Each box is 3 AMU wide and shows the peak shape for one mass. 4 Click Start to monitor the Agilent 7500 signals and view the spectra and numerical values in the real-time display.
  • Page 165 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning mass toward higher mass. • Axis Offset Adjusts mass calibration. The higher the value, shifts the peak position of all masses toward higher mass. • QP Bias Controls the speed of ions as they pass through the Q-pole. A typical value is 0 V without the ShieldTorch system.

  • Page 166: Tuning The Detector

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Tuning the Detector An electron multiplier (EM) is used as the detector. There are 2 modes for detecting signals and it is possible to measure samples with concentrations ranging from sub ppt up to hundreds of ppm.

  • Page 167: Discriminator

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Discriminator The discriminator is a threshold for determining the difference between general electrical noise from power supplies etc. and analyte signal. If it is set too low, the noise becomes high. If it is set too high, it cuts not only the noise, but also decreases the sensitivity.

  • Page 168: Prevention Of Em Deterioration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Prevention of EM deterioration When higher signals are analyzed for a long time with very high sensitivity, the EM may deteriorate fast. EM deterioration causes drift of the signal and the lifetime of the EM may be shortened.

  • Page 169: Setting P/A Factors

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Setting P/A Factors ChemStation automatically switches between pulse and analog mode. For linear calibration curves, these two modes should be adjusted by using P/A Factor tuning. Usually a standard solution that includes all analytes in your sample and will be used as one of the standard solutions to make calibration curves is used for tuning P/A Factors.
  • Page 170 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning For P/A Factor tuning, complete the following steps: 1 Aspirate tuning solution. 2 Select Tune>>P/A Factor. The P/A Factor Tuning dialog box appears. 3 Set the mass number and/or elements to be used for tuning.
  • Page 171 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning moving the sample probe of the autosampler. Uptake time and Stabilization time of the solution can be shortened when the solution washout time is sufficient. N O T E When “0” is entered in the ALS tuning solution vial box, the sample probe does not move to the Rinse Port after the tuning.
  • Page 172 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning there is no error in the P/A factor, it is updated to the latest P/A factor. Cancel the check of the Merge in the current data box when adjusting a new P/A factor.

  • Page 173: Generating A Tune Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Generating a Tune Report You can generate a tune report to keep a record of tuning results and tuning parameter values, which are lost when you exit the Tune window. The tune report provides data about Sensitivity, Resolution/Axis numerically and graphically, and all tuning parameters.

  • Page 174: Copying A Tune Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Copying a Tune Parameters You can use the Copy Tune Parameter dialog box to copy the tune parameters from the current file to another tune file or from another tune file to current setting.

  • Page 175: Creating And Using A Tune File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Creating and Using a Tune File ChemStation automatically saves the tuning parameters to a tuning file. Exiting the Tune window overwrites the most recent tune values to the default tune file, atune.u. However, you may want to save a set of parameters to use as a starting point for future tuning procedures, especially if you are tuning the instrument for a special type of analysis.

  • Page 176: Saving The Tuning File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Saving the Tuning File To create a tune file, save the tuning parameters by completing the following steps from the Tune window: 1 Select File>>Save Tune Values. The Save Tuning Parameter File dialog box appears.

  • Page 177: Loading A Tune File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Loading a Tune File To use a saved tune file for a tuning procedure, load the file by completing the following steps in the Tune window: 1 Select File>>Load Tune Values. The Load Tuning Parameter File dialog box appears.

  • Page 178: Autotune

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Autotune Autotune automates the optimization of various tunable lens and voltage settings to optimize the instrument. In Autotune it is possible to change the targeted sensitivity along with the range for each tuning parameter.
  • Page 179 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning To perform Autotune, complete the following processes: 1 Introduce a tuning solution. The standard tuning solution containing 10 ppb of Li, Y, Ce, Tl is recommended when you use the standard torch system.
  • Page 180 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning • Extraction Select when using ShieldTorch or when running hot plasma without the ShieldTorch. When selected, Extract 1 lens is set to a negative value for tuning. • Soft Extraction This is the autotune mode for reducing background when using the Shield- Torch.
  • Page 181 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning When selected, autotune automatically optimizes the torch vertical and horizontal position. Adjusted parameters: Torch-H, Torch-V • Lens/Plasma When selected, autotune automatically optimizes the ion lens parameters in accordance with the target values. Adjusted parameters:...
  • Page 182 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Set Autotune Targets Dialog Box • EM Set the mass number that will be used for gain adjustment in the detector (EM) tuning. When Auto Selection is selected, it automatically selects the mass used in the detector (EM) tuning.
  • Page 183 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning selected. • Sensitivity For the lower limit, select the Increase Sensitivity box and type the target value of the lower limit. For the upper limit, select the Decrease Sensitivity box and type the target value of the upper limit. (e.g. reducing the ArO(56amu) with the...
  • Page 184 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning When you click the Range Setting, the Parameter Range appears at the right side of the Set Autotune Target dialog box. In autotune it is possible to set a range for each variable tuning parameter value.
  • Page 185 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning box. N O T E Autotune tuning parameter settings can also be saved as an Autotune Target File. For the details, see the chapter “Creating and Using a Autotune Target File”. 9 Click Run in the Autotune dialog box.
  • Page 186 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning tuning progresses. The current tuning item will have a yellow colored status lamp indicating that it is the item being optimized. The Status Bar is meant to give a rough indication of the tuning progress.
  • Page 187 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning When autotune is completed, any message displayed during the execution of autotune will be indicated on the report pad. If an error was generated during the execution of autotune, autotune will be aborted and the details of the error will be displayed.

  • Page 188: Aborting Autotune

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Aborting Autotune Autotune is suspended with the Abort button during the execution of autotune. A confirmation dialog is displayed that allows 3 selections for “Saving the Current Tune Status”: Yes, No or Cancel. Cancel will continue the Autotune. No will close Autotune with no change in values and YES will save the current tune state.

  • Page 189: Creating And Using A Autotune Target File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Creating and Using a Autotune Target File The target values set in the Autotune Targets dialog box can be saved as an autotune target file. Saving the Autotune Target File To create an Autotune Target file, save the target values by completing the following steps from the Set Autotune Targets dialog box: 1 Click Save in the Set Autotune Targets Dialog box.

  • Page 190: Loading An Autotune Target File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Loading an Autotune Target File To use a saved Autotune Target file, load the file by completing the following steps from the Autotune Targets dialog box: 1 Click the load button in the Set Autotune Targets Dialog box.

  • Page 191: Peristaltic Pump Program For Autotune

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning Peristaltic Pump Program for Autotune When Autosampler is selected in the Configuration task, setting of the peristaltic pump program is required. Select Peri Pump Program for Autotune and the Peristaltic Pump Program dialog box appears. You can set the peristaltic pump speed or the stabilization time before and after Autotune.
  • Page 192 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tuning 4-116...
  • Page 193 Creating a Method...

  • Page 194: Creating A Method

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Creating a Method A method encompasses data acquisition and data analysis. Use them to define parameters for acquiring and analyzing data. The last method used before closing ChemStation is loaded into memory, next time you access the software.

  • Page 195: Creating A Method Using The Method Wizard

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Creating a Method Using the Method Wizard The method wizard is an easy way to create a method, even for beginners. Simply select an application and sample type, and a method suitable for the system configuration will automatically be created.

  • Page 196: Setting Method Filename

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Method Filename Set the name of a method file by the following procedure. 1 Select Methods>>Run Wizard…. The method wizard starts up. The list view appears on the left and the General Items page appears on the right.
  • Page 197 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Enter a method name and check the method path and system configuration. Enter a method name using up to 8 characters in the Method Name field. Add the extension .m. If the extension is not added, it will be automatically added to the filename.

  • Page 198: Selecting A Sample

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Selecting a Sample Select a sample on the Sample page by the procedure described below. 1 Select an application from Application Type. Application Type shows only the applications supported by the current system configuration.

  • Page 199: Editing Element Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Editing Element Information Element List Information on the Elements page shows the mass numbers and names of the elements for which data is to be acquired. When the acquisition mode is set to “Multi Tune,” the tune mode also appears.
  • Page 200 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 1 Add, edit, or delete an element. • When adding or editing, right-click on the element in Element List Information, and choose an option from the drop-down menu. Set the mass number (only when adding), the element name, and the integration time.
  • Page 201 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method N O T E When an interference correction equation has been edited, the change is also reflected in the mass number in Element List Information. 3 Click on Next. The Reports page appears.

  • Page 202: Editing Report Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Editing Report Information Edit the report information by the procedure described below. 1 Select a report style in Style. Reports page 2 Select the destination to which the report is to be output in Destination.

  • Page 203: Checking The Set Method

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Checking the Set Method The Finish page shows the details of the set method. Follow the procedure described below to check or edit the set method. 1 Check the details of the method and click on Finish.

  • Page 204: Using The Default Method As A Template

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Using the Default Method as a Template You can create a new method using the default method as a template. To create a method by editing the default method, you perform the following functions.

  • Page 205: Editing The Default Method

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 10 Save the method and calibration. This section includes a detailed explanation of each of these functions. Unless otherwise indicated, each function applies to all four acquisition types. Editing the Default Method To edit the default method, check the title bar of the Top window to be sure that the default method, DEFAULT.M, is currently in memory.

  • Page 206: Entering The Method Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method necessary method sections without going through the entire method. For more information, see “Modifying a Method” in this chapter. Entering the Method Information The Method Information dialog box enables you to choose which method sections to run, and also to write comments about the method.
  • Page 207 The pre-and post-run macro command check boxes enable you to execute actions before or after acquisition. Macros included with ChemStation allow automatic instrument startup and shutdown. Contact Agilent Technologies engineers for information about these as well as additional customized macros.

  • Page 208: Creating/Editing Interference Correction Equations

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Creating/Editing Interference Correction Equations Interference correction equations, if used in your method, should be edited prior to setting the acquisition parameters. This will ensure that all masses necessary for the interference corrections will be acquired. ChemStation enables you to modify or edit the Interference Equation.
  • Page 209 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 3 Select the mass for which you want to edit the interference equation by clicking on the mass number. The selected mass number will be highlighted. 4 Click the Edit. The Edit Equation dialog box will appear.
  • Page 210 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 5 Edit the equation in the table. For example, if the interference equation for As is: M x 1 + M x (-3.1278) + M x 1.0177 As = (where M represents 75 AMU), it would be edited.

  • Page 211: Setting The Acquisition Mode

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting the Acquisition Mode There are six acquisition modes available. You can choose which type of analysis you want to perform according to your application. ChemStation chooses Spectrum mode as default.

  • Page 212: Setting Acquisition Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Acquisition Parameters The following sections explain the six acquisition modes: Spectrum Using the Spectrum mode, you can perform qualitative, semiquantitative, and quantitative analysis. This section will explain how to set parameters for all three types of analysis.

  • Page 213: Setting Parameters For Qualitative Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Parameters for Qualitative Analysis Qualitative analysis provides information about which elements are included in the sample. It is very useful for pre-screening unknown samples. ChemStation scans through all masses (2-260AMU), and gives you a quick visual indication of what elements are present in the sample.
  • Page 214 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 3 Double-click on each of the three rows. ChemStation will select all masses (2-260 AMU) excluding masses 14, 16 to 19, 32, 36, 38, 40, and 41 AMU. You can tell that the masses are selected by the green vertical bars above the points on the mass scale.
  • Page 215 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method up box. Check Parameter Dialog Box 9 Click OK. All data acquisition parameters are now set. The screen goes on to Peristaltic Pump Program, explained later in this chapter. 5-23...

  • Page 216: Setting Parameters For Semiquantitative Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Parameters for Semiquantitative Analysis Semiquantitative analysis is a very powerful feature of ICP-MS, since it provides reasonable accuracy (±10 - 30%) without the need to run external standards. It scans through all masses (or selected masses) and reports concentrations for all elements, based on a stored elemental response database.

  • Page 217: Setting Parameters For Quantitative Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Parameters for Quantitative Analysis Quantitative analysis provides information about specific elements in a sample. You can quantitate the elements you selected by using calibration curves. The Masses box in the Spectrum Acquisition Parameters dialog box can display either the periodic table or the mass scale.
  • Page 218 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 3 Select elements by clicking the element push buttons in the periodic table. The push buttons for the elements you select turn from gray to red. The isotopes for each element are selected automatically according to the default recommended isotopes.
  • Page 219 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method The Element Information contains data on ionization potentials, melting point, boiling point, and isotopic abundance for the selected element. It also contains notes that provide additional information about the selected element, such as the element’s toxicity, solubility, and stability.
  • Page 220 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Interference Information provides information about isobaric interferences where elements have isotopes that overlap at the same mass. You can also view possible interferences from polyatomic ions by clicking in the check boxes at the bottom of the dialog box.
  • Page 221 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method The AMU Information contains information about the abundance of an element at a specific mass. You can check interferences from polyatomic ions by clicking in the check boxes at the bottom of the dialog box. The polyatomic ions of the selected specific type that could interfere with the masses displayed will be displayed.
  • Page 222 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 6 Click OK after selecting all isotopes you want to analyze for that element. ChemStation returns to the periodic table. The numbers of isotopes you selected are indicated in the right bottom corner of each element push button.
  • Page 223 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method AMU’s used in the interference equation at the Periodic Table Masses window, the element names in the Mass Table window will be displayed automatically and be put in parentheses. You can delete or add elements and AMU’s on this screen.
  • Page 224 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 9 Click OK. ChemStation will return to the Spectrum Acquisition Parameter screen. 10 Click the Full Quant (3) in the Peak Pattern (left bottom) box. ChemStation will display the 3 peak top points. The width of each bar is 0.05 AMU.
  • Page 225 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Spectrum Acquisition Parameters Dialog With Set Every Mass Panel 12 Set the Detector Mode. Click the masses in the list which you want to analyze using the analog mode. Then select Analog in the Detector mode box. Click Enter.
  • Page 226 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method telling you what the mistake is will pop up. Click OK on the pop up box. 15 Click OK. All data acquisition parameters are set as you edited them. The screen goes on to Peristaltic Pump Program, explained later in this chapter.

  • Page 227: Prevention Of Em Deterioration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Prevention of EM deterioration When higher signals are analyzed for a long time with too high sensitivity, the EM may deteriorate fast. EM deterioration causes the drift of the signal and the lifetime of the EM may be shorten.

  • Page 228: Time Resolved Analysis (Tra)

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Time Resolved Analysis (TRA) Time Resolved Analysis acquisition (TRA) produces a transient signal that appears as an intensity versus time plot (time chart). This acquisition mode is usually used when the signal changes with time (transient signal). You can monitor the signals including a time factor for each selected mass.
  • Page 229 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Click OK. ChemStation sets Time Resolved Analysis as the acquisition mode for the method and the Time Resolved Analysis dialog box appears. Resolved Analysis Acquisition Parameters Dialog Box 5-37...

  • Page 230: Setting Parameters For Time Resolved Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Parameters for Time Resolved Analysis Time Resolved Analysis provides information about changes in signals of specific masses with time. Because setting the parameters for TRA is similar to Spectrum Analysis mode, refer to “Setting Parameters for Quantitative Analysis”, earlier in this chapter, as well as...
  • Page 231 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Extract is selected, then the available masses will turn to black from gray; it will then be possible to change the order of the mass list. Real Time Plot Dialog Box 6 Input the X-axis for the real-time display by double-clicking or dragging on the text box for TimeWindow.
  • Page 232 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 9 Click Check Parameters. This is to make sure that you haven’t made any major errors, such as forgetting to select some of the masses to be acquired, etc. If there are no errors in the method, a dialog box will pop up to indicate so.

  • Page 233: Time Program

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Time Program ChemStation enables you to set acquisition parameters for up to 10 sections, known as steps, in a time program. This enables you to change any method parameters part of the way through a time resolved analysis. For example, you can change the masses or load a different tuning file at a specific time in the sample run.
  • Page 234 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Click OK. ChemStation sets Time Program as the acquisition mode for the method and the Time Program dialog box appears. Time Program Dialog Box 5-42...

  • Page 235: Setting Parameters For Time Program

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Parameters for Time Program To create and set a time program, complete the following steps. Please refer to “Time Program dialog box” in the Online Help. 1 To select a step for which to set parameters, click the step in the displayed list.
  • Page 236 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method the file. • Double-click a tune file in the displayed list. ChemStation will return to the Time Program dialog box. 4 To set the start time for a step, click Skip.
  • Page 237 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method ChemStation copies the parameters you set for the current step to the step you just highlighted and return to the Time Program dialog box. 6 Repeat Steps 1 through 5 for each step.

  • Page 238: Isotope Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Isotope Analysis Isotope Analysis is similar to Spectrum Analysis. The difference is that the dwell time per mass is 10 times shorter. This mode should be used when precision for iso- topic information (isotopic ratio and isotope dilution) is required (e.g.

  • Page 239: Setting Parameters For Isotope Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Isotope Analysis Acquisition Parameters Dialog Box Setting Parameters for Isotope Analysis Because Isotope Analysis is similar to quantitative analysis in Spectrum Analysis, setting the acquisition parameters is the same. Refer to “Setting Parameters for Quantitative Analysis”...

  • Page 240: Spectrum Multi Tune

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Spectrum Multi Tune For semiconductor applications, Cool plasma allow for K, Ca and Fe to be measured at low level. However Some elements with high ionization potentials can not measured by cool plasma because cool plasma can not ionize then efficiently, so the sensitivity is low.
  • Page 241 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Click OK. ChemStation sets Spectrum [Multi Tune] as the acquisition mode for the method and the Spectrum [Multi Tune] Acquisition Parameters dialog box appears. Spectrum [Multi Tune] Acquisition Parameters Dialog Box...

  • Page 242: Setting Spectrum [Multi Tune] Acquisition Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting Spectrum [Multi Tune] Acquisition Parameters Select the masses, Integration time and Peak Pattern for the elements to be acquired. Refer to Setting Parameters section discussed in the Creating a Method section of this manual.
  • Page 243 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Set the integration time for each tune step. The integration time can be set for multiple tune steps in each mass number. To set the integration time, click the integration time area which is crossed by the target mass number and the target tune step, then enter the integration time and click Enter.
  • Page 244 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Turn the Return to First Tune Step check box on when you want to reset the tuning parameters of the ICP-MS after finishing data acquisition. If the check box is off, after finishing the data acquisition, the tuning parameters of the ICP-MS are the tuning parameters in the last tune step file.

  • Page 245: Setup

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setup This document will give you an example of the operation procedure (cool and hot) from setting up the system to make a method using Multi Tune. 1 Rotary Pump Confirm that only one Rotary Pump is running when running cool plasma.
  • Page 246 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Install the shield plate Refer to pages 1-12 to 1-16 in “Agilent 7500 ICP-MS Option Instruction Manual” about shield torch installation. 3 Remove mass 40 AMU as one of the Prohibit masses at Configuration window...

  • Page 247: Making The Method

    Normal plasma tuning procedure Refer to Chapter 4 in “Agilent 7500 ICP-MS ChemStation Operator's Manual”. Cool plasma tuning procedure Refer to page from 1-25 to 1-30 in “Agilent 7500 ICP-MS Option Instruction Manual”. Making the Method 1 Go to Method >> Edit Entire Method...
  • Page 248 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Check the Method section to edit. 3 Enter Method comment as you like, then Click OK 5-56...
  • Page 249 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 4 Select Spectrum [Multi Tune], then click OK. 5 Acquisition [Multi Tune] parameters windows appear Click Periodic Table 5-57...
  • Page 250 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 6 Select masses you analyze, then Click OK. 7 Select Peak pattern [3] Click Tune Step 1 Click Browse… to select the Tune files 5-58...
  • Page 251 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 8 Select a tune file for Step 1 from the drop-down list in the upper right section. 9 Click Tune Step 2. 5-59...
  • Page 252 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 10 Enter the stabilization time (e.g., 30 sec) for Step 1 and Step 2 in the text boxes in the upper right section. N O T E The recommended stabilization time is for most applications: <30 seconds from cool plasma to hot...
  • Page 253 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 11 Select a tune step for each element. Refer to page 5-50 “Highlight the mass (see below) by clicking on the masses while holding down the Ctrl key”. N O T E...
  • Page 254 Creating a Method Click Check Parameter and confirm no errors reported. 12 Click OK. Refer to Saving a Method and Calibration on page 5-87 in “Agilent 7500 ICP-MS ChemStation Operator’s Manual”. 13 Enter calibration name, then click OK Creating this method is now complete.

  • Page 255: Isotope Analysis [Multi Tune]

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Isotope Analysis [Multi Tune] Isotope Analysis [Multi Tune] is similar to Spectrum Analysis [Multi Tune]. The difference is that the dwell time per mass is 10 times shorter. This mode should be used when precision of isotopic information (isotopic ratio and isotope dilution) is required (e.g.

  • Page 256: Setting Parameters For Isotope Analysis [Multi Tune]

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Isotope Analysis [Multi Tune] Acquisition Parameters Dialog Box Setting Parameters for Isotope Analysis [Multi Tune] Because Isotope Analysis [Multi Tune] is similar to quantitative analysis in Spec- trum Analysis [Multi Tune], setting the acquisition parameters is the same. Refer to “Setting Parameters for Quantitative Analysis”...

  • Page 257: Setting The Peristaltic Pump Program

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Setting the Peristaltic Pump Program You can determine the speed at which the peristaltic pump runs, and set a delay to allow for the time it takes for the sample to reach the plasma and stabilize. If the ASX-500 or ASX-100 is set as the autosampler in the ICP-MS Configuration, then the window to set up the time and pump speed during the rinse is added.
  • Page 258 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method ChemStation allows you to set Intelligent Rinse for the post-acquisition rinsing. The probe rinse normally begins after the termination of acquisition. However, you can also start the rinse before acquisition ends.
  • Page 259 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 4 To use the autosampler, enter the settings for the post-acquisition probe rinse in the After Acquisition (Rinse Port) section. Rinse solution in the rinse port of the autosampler is taken up into the nebulizer.
  • Page 260 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 3) To input mass data directly, enter mass values in the left-side text boxes under “Mass or Ratio.” 4) To load masses from acquisition parameters, click Load Masses from Acq. Parameters.
  • Page 261 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 7 Do the following to start the rinse before the end of acquisition: 1) Select the Execute Pre-emptive Rinse checkbox. 2) In the Pre-emptive Time text box, set the number of seconds before the end of acquisition at which you wish to start the rinse.

  • Page 262: Setting Corrections For The Method

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 8 To accept the values you set for the peristaltic pump program, click OK. The Set Correction dialog box appears. Setting Corrections for the Method ChemStation enables you to set corrections for both background and isobaric inter- ferences.
  • Page 263 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 4 Select the box, Rejected Masses and enter the masses for which background spectrum you do not want subtracted. The background spectrum for the masses you edited in the Rejected Masses box will not be subtracted.

  • Page 264: Selecting Reports

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Selecting Reports ChemStation gives you several choices for generating reports as part of the method. You can generate reports to the screen, to the printer, or to a file that you specify.
  • Page 265 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To select the type of report you want to generate when the quantitation method runs, complete the following steps: 1 Select a report format in the Quantitation Report area. To generate the default quantitation report, select the box for Quantitation Report .
  • Page 266 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method b) To see the list of default report styles for FullQuant, click on the arrow next to Style. Three default report styles are available. For more information about report styles, please refer to appropriate sections of Chapters 12.
  • Page 267 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To generate a custom report template for quantitative analysis when the method runs, complete the following steps: a) The Select Custom Report Template (FullQuant) dialog box appears. Select Custom Report Template (FullQuant) Dialog Box b) Select the custom report template (.fqt extension) in one of the following...
  • Page 268 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To update a custom report database for quantitative analysis when the method runs, complete the following steps: a) The Select Custom Report Database (FullQuant) dialog box appears. Select Custom Report Database (FullQuant) Dialog Box b) Select the custom report database (.fqd extension) in one of the following...
  • Page 269 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To generate a QC Report, complete the following steps: N O T E If your QC configuration does not include any QC parameters, the QC Report section will be grayed out.
  • Page 270 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To select the type of report you want to generate when the semiquant method runs, complete the following steps: 1 Select a report format in the Semiquant Report area. To generate the default semiquant report, click the Semiquant Report box and click To generate a custom report template for semiquantitative analysis when the method runs, click the Custom Report box and click OK.
  • Page 271 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method b) To see the list of default report styles for SemiQuant, click on the arrow next to Style. Two default report styles are available. For more information about report styles, please refer to appropriate sections of Chapters 13.
  • Page 272 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method a) The Select Custom Report Template (SemiQuant) dialog box appears. Select Custom Report Template (SemiQuant) Dialog Box b) Select the custom report template (.sqt extension) in one of the following ways: •...
  • Page 273 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To update a custom report database for semiquantitative analysis when the method runs, complete the following steps: a) The Select Custom Report Database (SemiQuant) dialog box appears. Select Custom Report Database (SemiQuant) Dialog Box b) Select the custom report database (.sqd extension) in one of the following...
  • Page 274 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To generate an Isotope Ratio Report, complete the following steps: 1 Select the Isotope Ratio Report check box in the Isotope Report area. 2 To produce a batch file of the report, select the check box Copy saved Text Reports to ‘Reports’...
  • Page 275 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method • Printer Generates the report and sends it to the ChemStation printer. • File Generates the report and saves it as a file. Use the dialog box to give this file a name.
  • Page 276 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method To generate an Isotope Dilution Analysis Report, complete the following steps: 1 Select the Isotope Dilution Report check box in the Isotope Report area. 2 To produce a batch file of the report, select the check box Copy saved Text Reports to ‘Reports’...

  • Page 277: Selecting The Edit Analysis Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method You can click one or more of the following boxes: • Screen Generates the report and displays it on the ChemStation screen. • Printer Generates the report and sends it to the ChemStation printer.

  • Page 278: Editing Qc Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method If you select SemiQuant and click OK, the SemiQuant Parameters dialog box appears, followed by the Internal Standard Correction (Normal Mode) and Blank Conc. Subtraction (SemiQuant) dialog box. If you select IR and click OK, the IR Parameters dialog box appears.

  • Page 279: Saving A Method And Calibration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Saving a Method and Calibration To save the selected method and calibration just created, complete the following steps: 1 Select the Method Save Options by clicking on the boxes for each option.
  • Page 280 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 3 Give your new Calibration a name and click OK. The name can be up to eight characters long. Do not use the following charac- ters in the method name: Period (.)
  • Page 281 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 4 Give your new Method a name and click OK. The name can be up to eight characters long. Do not use the following characters in the method name: Period (.)
  • Page 282 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 2 Select the Calibration Save Options by clicking on the boxes for each option. There are 2 options available on the Calibration Save Options dialog box: • Click the Alert when Calib is Overwritten box if you want the ChemStation to alert you with a message when an existing calibration will be overwritten.

  • Page 283: Modifying A Method

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Modifying a Method ChemStation enables you to easily modify specific parameters in a method without having to edit the entire method. You can edit the method information, data acquisi- tion parameters, data analysis sections, and QC parameters of the method separately.

  • Page 284: Loading A Method And Calibration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Loading a Method and Calibration Before you can modify a method, you must first load it into memory. To do so, com- plete the following steps: 1 Select Top>>Methods. The Methods menu appears.
  • Page 285 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method The Load Calibration File dialog box appears. Load Calibration File Dialog Box 4 To load the calibration, select it in one of the following ways: • Type the name of a calibration and click OK. If the directory path in the Load Calibration dialog box is incorrect, you must type the directory path as well as the name of the calibration.

  • Page 286: Modifying The Method Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Modifying the Method Information If you do not want to change the method parameters, but want to add comments about the method or change the conditions for running and saving the method, you can edit the method information section of the method separately.

  • Page 287: Modifying Data Acquisition Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Modifying Data Acquisition Parameters If you want to change only the data acquisition parameters of a method, ChemStation enables you to change the parameters from the ICP-MS Acquisition window. To do so, complete the following steps: >>...
  • Page 288 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method method until you save the method. 4 To change acquisition parameters, select Edit Parameters>>Set Parameters. Set Parameters opens Acquisition Parameters dialog box, where represents the selected acquisition mode. For more information about the parameters that appear in the Acquisition Parameters dialog box, see the appropriate section earlier in this chapter.
  • Page 289 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method The Methods menu appears. 3) Select Methods>>Save. The Method Save Options dialog box appears. Save the method as described in Saving a Method and Calibration earlier in this chapter. >> N O T E...

  • Page 290: Printing A Summary Of Method Acquisition Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Printing a Summary of Method Acquisition Parameters ChemStation enables you to print a summary of the acquisition parameters for each method you create. The summary also includes error messages that ChemStation generates if appropriate.

  • Page 291: Modifying Amu Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Modifying AMU Information ChemStation enables you to modify the AMU Select File. The AMU Select File is the file where you can edit the isotopes to be pre-selected when you click an element push button on the periodic table.
  • Page 292 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method AMU Select File Dialog Box 4 Click on the isotope (mass number) you want to select or deselect then click the Set/Reset button. The masses selected will be indicated in < > (brackets). The masses without brackets are not selected.
  • Page 293 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Save AMU Select File Dialog Box 6 Save the AMU Select File. If you want to save the changes to the AMU Select File you loaded, click OK. If you want to save the changed AMU Select File as a new file, type in the new file name and click OK.

  • Page 294: Modifying Data Analysis Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Modifying Data Analysis Parameters ChemStation enables you to change each parameter separately. To do so, complete the following steps: >> 1 Select Top Data Analysis. The Data Analysis menu appears. >>...
  • Page 295 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method For more information about editing these data analysis parameters, please refer to the appropriate sections of Chapters 12-15. The changes you make are not recorded to the method until you save the method.

  • Page 296: Printing A Summary Of Data Analysis Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Printing a Summary of Data Analysis Parameters ChemStation enables you to print a summary of the data analysis parameters for each method you create. To print a summary of the data analysis parameters for a method, complete the fol- lowing steps: >>...

  • Page 297: Modifying Qc Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method Modifying QC Parameters To edit the QC Database and QC Tune Criteria parameters, please refer to the appropriate sections of the Intelligent Sequence Manual. 5-105...
  • Page 298 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating a Method 5-106...

  • Page 299: Setting Up A Sequence

    Setting Up a Sequence...
  • Page 300 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Setting Up a Sequence A sequence is designed to allow you to analyze a number of samples automatically. Multiple methods can be used within a single sequence, if desired. Also, you can run a sequence, either with or without using an autosampler.

  • Page 301: Creating A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Creating a Sequence To create a sequence, use the Sample Log Table to enter information that the Agilent 7500 uses when analyzing samples. The samples are analyzed in the order in which they appear in the Sample Log Table.
  • Page 302 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Sample Log Table Dialog Box 2 Set the method name by double-clicking the cell, and selecting the appropriate method in Select File dialog box. ChemStation uses the method to analyze the sample. Although a different method can be used for each sample in your sequence, normally you will use the same method for all samples in a sequence.
  • Page 303 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence • CalStd Select this type when updating an existing calibration table in the specified method. Usually this type is used for standard solutions. The calibration table should have been created (see Chapter 12) prior to beginning the sequence. When this type is selected, the appropriate calibration level in the calibration table must be selected.
  • Page 304 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence ALS GoTo Dialog Box 5 Set a file name by clicking the cell, and typing. The data file name can be up to eight characters long. It must not contain the following characters: Period (.)
  • Page 305 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence If you do not enter a data file name, ChemStation automatically assigns one. The system-assigned name is a 3-digit number with the sample type specific suffix. A 3 digit number is a run counter beginning at 001 for the first sample analyzed and incremented by 1 for each subsequent sample.
  • Page 306 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence The Calculate Dilution Factor dialog box appears. Calculate Dilution Factor Dialog Box 5) Enter a numerical value in each text box. 6) Click Calculate. The dilution factor will be automatically calculated.
  • Page 307 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence 2) To select a concentration from the calibration levels, choose the Select a Calibration Level radio button and select a level from the drop-down list. Proceed to step 5). 3) To set a specific value for the current sample, select the Set Specific Value radio button.
  • Page 308 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence 3) Set an LC/GC vial number in the LC/GC Vial field. If you leave the LC/GC Vial field blank, the method in the LC/GC ChemStation will not be executed. You can set a vial number from -1 to 200. (The range of numbers you can set varies depending on the instrument connected.)
  • Page 309 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence You can set up the sequence by directly loading a CSV file (comma separated value ASCII file). Select Load List from CSV file from the popup menu, and then the Open dialog box appears.
  • Page 310 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Enter the name of the CSV file, and then click Save. The following figure shows the relationship between the Sample Log Table and the CSV file. If there is a entry which you want to leave blank, a comma should be set for that in the CSV file.

  • Page 311: Position And Run

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Position and Run You can start the sequence from the middle of the Sample Log Table. To position and run a sequence, complete the following steps: 1 Select Sequence>>Position and Run.

  • Page 312: Simulating A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Simulating a Sequence You can check a sequence before running it by “simulating” the sequence. The simulate sequence command runs through the sequence to check for duplications in file names, incorrect directory destinations, and to check whether there is enough disk space available to store your data.
  • Page 313 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence 2 Select a file using one of the following methods: • Click a file name in the displayed list and click OK. • Double-click a file name in the displayed list.
  • Page 314 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Run Sequence Status Box Confirmation Dialog Box N O T E Click OK to accept the edited information without simulating the sequence. Click Cancel to cancel the edited information. 7 Click OK.

  • Page 315: Viewing The Sequence Log File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Viewing the Sequence Log File The Sequence Log file is generated every time a sequence is simulated or run. The file contains a list of vial numbers, names of data files, and any error messages gen- erated during the sequence.
  • Page 316 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence 2 To print the sequence log file, select File>>Print from the Report Pad menu. ChemStation sends the file to the printer. You can also print the Sequence Log by selecting Print Sequence Log from the Sequence menu.

  • Page 317: Modifying A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Modifying a Sequence You can modify a sequence when you edit the Sample Log Table, and also by select- ing different Sequence Options. Editing Sample Log Table to Modify a Sequence Before you can edit a Sample Log Table for the sequence you want to modify, you must load the sequence into memory.

  • Page 318: Modifying Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Modifying Sequence You can also modify a sequence without editing the Sample Log Table. ChemSta- tion provides additional options to change a sequence: re-sequencing data files and changing the method. When you use these sequence options, ChemStation automat- ically changes information in the Sample Log Table in the manner specified.

  • Page 319: Saving The Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence Saving the Sequence After the changes are made to the Sample Log Table, you can save the changes to disk by completing the following steps: 1 Select Sequence>>Save. The Save Sequence dialog box appears.
  • Page 320 Agilent 7500 ICP-MS ChemStation Operator’s Manual Setting Up a Sequence 6-22...

  • Page 321: Chained Sequence

    Chained Sequence...
  • Page 322 Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence Chained Sequence A chained sequence is designed to allow you to run a series of sequences. Also, you can assign different tuning parameters to each sequence. Any sequences used must have the appropriate sample log table set prior to running a chained sequence.

  • Page 323: Creating A Chained Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence Creating a Chained Sequence To create a chained sequence, complete the following steps from the ICP-MS Top window: 1 Select Chained Sequence>>Edit and Run. The Chained Sequence Table dialog box appears. To create a sequence, you edit the sample log table for the chained sequence currently in memory.
  • Page 324 Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence 2 Set the following items of the chained sequence table. • Sequence File… Double-click the Sequence File field you want to set. The Select Sequence File dialog box appears. Select an existing sequence file you want to run.
  • Page 325 Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence • Data Batch… When the “Sequence File” area is left without any name, a new data batch directory as the destination for the data files is created in the c:\icpchem\1\data at the time you begin your sequence using Full Method.
  • Page 326 Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence • Tune File… You must choose the tune file for each sequence. Double-click a Tune File field you want to set. The Select Tuning File dialog box appears. Select an existing tuning file you want to use.
  • Page 327 Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence 3 Selecting Other items if needed. There are two options for running a chained sequence. • Save Sequence after Run… When it is on, the sequence modified during the run can be overwritten after run- ning the sequence.

  • Page 328: Simulating A Chained Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence Simulating a Chained Sequence You can check a chained sequence before running it by “simulating” the chained sequence. The simulated chained sequence command runs through each sequence line to check for duplications in file names, incorrect directory destinations, and also to check whether there is enough disk space available for your data.

  • Page 329: Saving The Chained Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence Saving the Chained Sequence After the changes are made to the chained sequence table, you can save these changes to disk by completing the following steps: 1 Select Chained Sequence>>Save. The Save Chained Sequence dialog box appears.
  • Page 330 Agilent 7500 ICP-MS ChemStation Operator’s Manual Chained Sequence 7-10...
  • Page 331 Running a Sample Analysis...

  • Page 332: Running A Sample Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Running a Sample Analysis You can run samples through the Agilent 7500 in one of two ways: • Running a sequence enables you to determine the order in which multiple samples are analyzed automatically.

  • Page 333: Running Samples By Using A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Running Samples by Using a Sequence Use a sequence when you want to analyze a large number of samples automatically. The arrangement of samples in the autosampler must match the arrangement you set up in the sample log table.

  • Page 334: Loading A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Loading a Sequence To load an existing sequence, complete the following steps from the Top window: >> 1 Select Sequence Load. The Load Sequence dialog box appears. Load Sequence Dialog Box 2 Use one of the following methods to choose the file name: •...

  • Page 335: Running A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Running a Sequence Running a sequence starts the sample analysis. To run a sequence that is already loaded, complete the following steps from the Top window: 1 Confirm that the currently loaded method at the Top level is saved in the same directory as the method to be used for reprocessing.
  • Page 336 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis 3 Select method sections to run. Select Full Method if you are ready to acquire data. Select Reprocessing Only if the data has already been acquired (e.g. if the data has been acquired manually, or if you are reprocessing data files acquired previously in a sequence).
  • Page 337 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Run Sequence Status Box N O T E Urgent samples can be appended to or inserted into the sample log table, even when the sequence is running. Click on the Edit Sample Log Tbl push button. The log table will be displayed.

  • Page 338: Running A Sample By Using A Chained Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Running a Sample by Using a Chained Sequence A chained sequence is designed to allow you to run a series of sequences. To run samples using a chained sequence, you must first load the chained sequence and then run it.

  • Page 339: Running A Chained Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Running a Chained Sequence Running the chained sequence starts the sample analysis. To run a sequence that is already loaded, complete the following steps from the Top window: 1 Select Chained Sequence>>Edit and Run.

  • Page 340: Running Samples Manually

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Running Samples Manually You can run a method to analyze samples if you do not have an autosampler. However, you may have other reasons to run a method rather than a sequence. For example, if you have only one or two samples to analyze, it may be more efficient to run the samples manually rather than set up the autosampler.
  • Page 341 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis 2 To load the method, select it in one of the following ways: • Click a file name in the displayed list and click OK. • Double-click a file name in the displayed list.

  • Page 342: Acquiring Sample Data

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Acquiring Sample Data After loading the method, there are two ways to run a sample manually, Method Run and Acquired Data. To use Method Run, complete the following steps from the Top window: 1 Select Methods>>...
  • Page 343 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis The Calculate Dilution Factor dialog box will appear. Calculate Dilution Factor Dialog Box 2) Enter a numerical value in each text box. 3) Click Calculate. The dilution factor will be automatically calculated.
  • Page 344 ChemStation is equipped with macros that enable automatic startup and shutdown of the equipment. Contact your local Agilent Technologies for information on additional customized macros. 8 Click Run Method.
  • Page 345 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Run Method Status Box After the data acquisition, the data analysis runs when the Data Analysis check box in the Start Run dialog box is checked. N O T E After Run Method is executed, the method is automatically saved.
  • Page 346 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis To use Acquired Run, complete the following steps from the Top window: 1 Select AcquireData>>Main Panel. The ICP-MS Acquisition window appears. >> 2 Select AcquireData Acquire Data. The Acquire Data dialog box appears.
  • Page 347 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis The Calculate Dilution Factor dialog box will appear. Calculate Dilution Factor Dialog Box 2) Enter a numerical value in each text box. 3) Click Calculate. The dilution factor will be automatically calculated.
  • Page 348 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Proceed to step 5). 3) To set a specific value for the current sample, select the Set Specific Value radio button. 4) Set the concentration in the Conc field in the Current ISTD Conc table.

  • Page 349: Monitoring A Run

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Monitoring a Run The Real-Time display in the ICP-MS Acquisition window allows you to view a run in progress. Samples analyzed using a method for spectrum acquisition will show a display covering the full range of masses.
  • Page 350 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Real-Time Display Window for Spectrum Acquisition Real-Time Display Window for Time-Resolved Analysis Click on Snapshot to display snapshot data on the online data analysis screen. Using this function, an interim analysis can be performed during long-term analysis.

  • Page 351: Altering The Display

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis N O T E The detector mode is reflected in the acquired data when ChemStation finishes data acquisition. Therefore, snapshot data cannot save the correct detector mode. The mass number, for which Analog is selected in Detector Mode, are handled as data acquired in the analog mode, and the other mass numbers are handled as data acquired in the pulse mode.

  • Page 352: Stopping A Run

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Stopping a Run Stopping a Run from the Real-Time Display Window To stop a run from the Real-Time display before the run time elapses, click the Stop Run push button. The Agilent 7500 will stop in the middle of the current repetition.

  • Page 353: Stopping A Run By Stopping A Sequence

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis Stopping a Run by Stopping a Sequence If you want to stop the sequence you are currently running, click the Abort push button from the Run Sequence status window. A dialog box will pop up asking if you are sure you want to abort.
  • Page 354 Agilent 7500 ICP-MS ChemStation Operator’s Manual Running a Sample Analysis 8-24...
  • Page 355 Viewing Spectra...

  • Page 356: Viewing Spectra

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Viewing Spectra The Spectrum acquisition mode displays data acquired from sample analysis as intensity versus mass (mass spectrum). ChemStation generates spectra for data acquired with a method using Spectrum or Isotope Analysis as the acquisition mode.

  • Page 357: Displaying Spectra

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Displaying Spectra When you acquire data with a method for Spectrum acquisition and then access data analysis directly from data acquisition, ChemStation displays that data file. If you access the Data Analysis window from the Top menu, the Data Analysis window displays the ICPDEMO.D data file which is indicated in the title bar.

  • Page 358: Loading A Data File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Loading a Data File To load a different data file, complete the following steps: >> 1 Select Top Data Analysis>>Main Panel. The ICP-MS Data Analysis window appears, showing a display of the ICPDEMO.D data file.
  • Page 359 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra >> 2 Select Data File Load. The Load Data File dialog box appears, showing the path for the data file directory. Allows the user to select the data file to be loaded into data analysis for processing.
  • Page 360 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Selecting Data File>>Next Data File will load the data file that alphabetically follows the current data file in the same directory. For example, if the directory contains the following files: Soil01.d Soil02.d Soil03.d...

  • Page 361: Changing The Display Type

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Changing the Display Type ChemStation provides options for changing three aspects of the spectral display type. You can choose whether the Y-axis display is logarithmic or linear, whether the display reflects total counts or counts per second (CPS), and whether the display uses a bar or line graphic mode.

  • Page 362: Using The Display Count Box

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Using the Display Count Box Additionally, you can view the counts acquired for each point by using the Count Box. This ChemStation feature gives you an easy method to check the total counts or CPS at any point in each peak.

  • Page 363: Changing The Vertical Scale Of A Spectral Display

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Changing the Vertical Scale of a Spectral Display The scale for each row of the spectrum appears to the left of the row, and the number in the scale box indicates the scale of the current display. Increase or decrease the vertical scale as required.

  • Page 364: Changing The Horizontal Scale Of A Spectral Display

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Changing the Horizontal Scale of a Spectral Display Just as you can change the vertical scale of a spectral display, you can also change the horizontal scale. You can change the horizontal scale of the entire display or you can zoom in on one part of the display.
  • Page 365 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra To zoom in on one section of the display, complete the following steps: 1 Drag across the section of the display you want to enlarge. The section is highlighted. 2 Release the mouse button.

  • Page 366: Analyzing Spectra

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Analyzing Spectra To analyze spectra in ChemStation, you can access information from the element databases to identify unknown peaks. You can then match this information with a spectrum by using a template that overlays a selected peak on the spectrum.
  • Page 367 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra 3 Click AMU Info. The ICP-MS DATABASE [AMU Information] dialog box appears, listing informa- tion about the abundance of elements at specific masses. ICP-MS DATABASE (AMU Information) Dialog Box ChemStation displays the mass you selected in red.
  • Page 368 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Library Matching Template (e.g. Ba) 6 To place more templates, repeat the above steps. The templates for additional elements appear. The template for each element has a unique color and shows the element name at the top of Base AMU. The templates can be displayed up to ten elements.
  • Page 369 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra To delete a template from the spectral display, complete the following steps: 1 Double-click with the right button on a peak with a template overlay. The Library Matching dialog box appears. 2 Click Delete in the dialog box.

  • Page 370: Labeling Spectra

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Labeling Spectra You may want to label a spectrum after you have added templates to it or before you print it. ChemStation enables you to name or add identifying information to a spec- trum, a feature which is useful if you want to save a hard copy of a spectrum for future reference.

  • Page 371: Printing Spectra

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra N O T E You can change and delete the text by clicking exactly on top of the previously labeled point, rewriting the text, then clicking Update in the Annotate dialog box.

  • Page 372: Subtracting Background Spectra

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Subtracting Background Spectra It is sometimes useful to be able to subtract background polyatomic species to visu- ally inspect a spectrum. ChemStation can subtract any data file from another. This background correction will subtract the abundance of each mass in the background spectrum from the abundance of the corresponding mass in the spectrum of the cur- rent data file.

  • Page 373: Viewing A Tabulated Spectrum

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Viewing a Tabulated Spectrum ChemStation enables you to view results from sample analysis in tabulated as well as graphic form. Two modes of tabulation are available for each data file you load into the Mass Spectrum display window.
  • Page 374 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Points per Peak Tabulation The tabulation provides information about the mass, counts per second (CPS), count, and detector mode for each point per peak of each element analyzed. The amount of information contained in the tabulation depends on the peak pattern set and number of elements selected in the method.

  • Page 375: Performing A Mass Tabulation

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Performing a Mass Tabulation ChemStation also provides detailed information about each mass in a sample. You can view this detailed information by performing a mass tabulation. Before you can perform any tabulation, you must load a data file into the Data Anal- ysis window if one is not already showing.
  • Page 376 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra The tabulation provides information about the mass, element name, counts per second (CPS), detector mode, repetition, SD, RSD and integration time for each element analyzed in the sample. You may occasionally want to save a tabulated file to work with at a later time. To do so select File>>Save As and give the file with a new name.

  • Page 377: Editing And Printing Out The Header Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Editing and Printing Out the Header Information ChemStation enables you to edit the header information (the same information you edited when you acquired data) in the ICP-MS Data Analysis window. To edit the header, complete the following steps: 1 Select Data File>>Edit Header.
  • Page 378 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra The Calculate Dilution Factor dialog box will appear. Calculate Dilution Factor Dialog Box 2) Enter a numerical value in each text box. 3) Click Calculate. The dilution factor will be automatically calculated.
  • Page 379 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Proceed to step 5). 3) To set a specific value for the current sample, select the Set Specific Value radio button. 4) Set the concentration in the Conc field in the Current ISTD Conc table.

  • Page 380: Displaying Replicate Files

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Displaying Replicate Files When more than one repetition is set in a method for spectrum acquisition, Chem- Station creates a data file, or replicate, for each repetition. To view each individual replicate, complete the following steps: 1 Select Data File>>Display Replicate Files.

  • Page 381: Editing Spectral Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Editing Spectral Information ChemStation enables you to transfer data to a database to view individual replicates, edit data files, and other data from the ICP-MS Data Analysis window. This section explains how to transfer data files to the database, and how to work on the work- book.

  • Page 382: Editing Header Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Editing Header Information You are able to edit the header information on the database spreadsheet. Edit the header information on the Header sheet (indicated at the bottom of the work sheet). The sample name, operator, miscellaneous information and dilution factor can be edited.
  • Page 383 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra The dilution factor will be automatically calculated. 4 Click OK. The Calculate Dilution Factor dialog box will close and the dilution factor will be set. 9-29...

  • Page 384: Viewing And Editing Replicate Files

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Viewing and Editing Replicate Files You are able to view and edit replicate files individually on the workbook. Editing the replicate files includes excluding certain data from the average file in case the data was not acceptable.
  • Page 385 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra To include the element you excluded on a certain replicate file, or to include a whole replicate file that you excluded from the average file, complete the following steps: 1 Place the cursor on the element or any other cell in the same row you want to include and click.

  • Page 386: Tabulating The Spectral Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Tabulating the Spectral Information You can tabulate the spectral information on one sheet. The information to be tabu- lated are: mass, element, average counts per second (CPS), number of repetitions, standard deviation (SD), relative standard deviation (RSD), detector mode, integra- tion time, and the counts per second data for each replicate file.

  • Page 387: Drawing A Graph From The Tabulated Information

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra Drawing a Graph from the Tabulated Information Once you have tabulated the spectral information, you can draw a graph for the ele- ments of interest. To draw a graph for the elements of interest, complete the following steps: 1 Select the cells for elements of interest in the Table work sheet.
  • Page 388 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing Spectra N O T E You can save what you edited on the workbook by selecting DataFile>>Save. Data can be saved as text by selecting DataFile>>Save as Text. N O T E You can load a different data file by selecting DataFile>>Open, then selecting a file.
  • Page 389 Viewing a Time Chart...

  • Page 390: Viewing A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Viewing a Time Chart A time chart displays data acquired from sample analysis as intensity versus time. ChemStation generates a time chart for data acquired with a method using Time Resolved Analysis or Time Program as the acquisition mode.

  • Page 391: Displaying A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Displaying a Time Chart When you acquire data with a method for Time Resolved Analysis or Time Program acquisition and then access Data Analysis directly from Data Acquisition, ChemSta- tion displays the time chart you just acquired. If you access the Data Analysis win- dow from the Top menu, the Data Analysis window displays the ICPDEMO.D data...

  • Page 392: Loading A Data File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Loading a Data File To load a different data file, complete the following steps: >> 1 Select Top Data Analysis>>Main Panel. The ICP-MS Data Analysis window appears, showing a display of the ICP- DEMO.D data file.
  • Page 393 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart >> 2 Select Data File Load. The Load Data File dialog box appears, showing the path for the data file directory. Allows the user to select the data file to be loaded into data analysis for processing.
  • Page 394 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Selecting Data File>>Next Data File will load the data file that alphabetically follows the current data file in the same directory. For example, if the directory contains the following files: Soil01.d...
  • Page 395 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Total Ion Chart Display 10-7...

  • Page 396: Changing The Chart Type

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Changing the Chart Type The Total Ion Chart is the default time chart shown in the ICP-MS Data Analysis display window when an appropriate data file is loaded. It provides a single intensity versus time plot for all elements analyzed in a sample.
  • Page 397 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart The selected element appears in the box on the left side of the dialog box. To select more than one element, hold down the Control key and click the elements in the displayed list or drag in the list, then click Add.
  • Page 398 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart N O T E You can also view mass spectrum information for any time slice in a time chart. To do so, position the cursor at the time of interest in the Time Chart display and double-click the right mouse button.

  • Page 399: Using The Display Count Box

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Using the Display Count Box Regardless of which display type you have selected, you can view the counts recorded for each time on the Time Chart display by using a count box. This ChemStation feature enables you to quickly view the intensity at any given time slice.

  • Page 400: Changing The Vertical Scale Of A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Changing the Vertical Scale of a Time Chart Whether you are viewing a Total Ion Chart or an Extracted Ion Chart, ChemStation enables you to change the vertical scale of the display. The scale for each chart appears to the left of the chart, and the number in the scale box indicates the scale of the current display.

  • Page 401: Changing The Horizontal Scale Of A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Changing the Horizontal Scale of a Time Chart Just as you can change the vertical scale of a Time Chart display, you can also change the horizontal scale. However, you change the horizontal scale of a Time Chart by zooming in on one part of the display.

  • Page 402: Using The Overlay Feature

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Using the Overlay Feature ChemStation provides an overlay feature that enables you to view signals for several Extracted Ion Charts in one chart. For example, if you have four Extracted Ion Charts showing in the display window, you can use the overlay feature to change the display to one chart showing a signal for each of the four masses.
  • Page 403 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Extracted Ion Chart in Overlay Display N O T E You can change the scale of the display to view individual signals. To adjust the vertical scale of the Overlay display, follow the instructions given in “Changing the Vertical Scale of a Time Chart”...

  • Page 404: Labeling A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Labeling a Time Chart You may want to label the Total Ion Chart created by integrating results for elements in a sample. You can also label an Extracted Ion Chart. ChemStation enables you to name or add identifying information to a time chart prior to printing.

  • Page 405: Display Of Data At Points Of Time

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart N O T E Exiting the Data Analysis window or loading the another file erases the annotation. Display of Data at Points of Time During data analysis, data acquired at specific points of time can be displayed.

  • Page 406: Exporting Data

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart CPS Data Exporting Data You can save the data file currently displayed in the window in AIA format or as LC/MSD raw data. 1 Select Export AIA format for Agilent LC/GC or Export Agilent LC/MSD raw data from the Data File menu.

  • Page 407: Printing A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Printing a Time Chart You can print a time chart display at any time during the analytical process. For example, if you have changed the horizontal scale to show only the acquisition period from 50 to 100 seconds, you can print the resulting time chart display.

  • Page 408: Analyzing Results Using A Time Chart

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Analyzing Results Using a Time Chart ChemStation enables you to define integration parameters for the peaks in a sample and to integrate them to obtain concentration values. You can use these two features to examine the data acquired with a method for Time Resolved Analysis or Time Program acquisition.

  • Page 409: Integrating Acquisition Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart 3 To select a correction, enter a value in points in the Correction box. To compensate for a sloping baseline, you can adjust the integration window by selecting different points. The points relate to the number of time slices either side of the window that the baseline is measured.
  • Page 410 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Integration Parameters Dialog Box 2 3 Click the Current Data or Acq. Method. Current Data shows in the dialog box the masses acquired in the current file. Acq. Method shows the masses set by the current acquisition parameters. Select Acq.

  • Page 411: Integrating Results

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart Integrating Results Integrating the total ion or extracted ion chart gives total area counts for all the inte- gration windows selected. To integrate results for elements in a sample, complete the following steps: 1 Select Data Analysis>>Main Panel.
  • Page 412 Agilent 7500 ICP-MS ChemStation Operator’s Manual Viewing a Time Chart 10-24...
  • Page 413 Creating Custom Reports/Database...

  • Page 414: Creating Custom Reports/Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Creating Custom Reports/Database Custom Reports/Database allows you to: • Create a report with a customized layout. • Create a database for recording the statistical and graphical results of multiple analyses. You design the Custom reports and Custom databases by creating templates. After...

  • Page 415: Creating A Custom Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Creating a Custom Report You can use ChemStation’s Custom Report feature to create reports that includes only the information needed. Create a report template for each method you use. This section explains how to create a Custom Report and how to save the report as a template.
  • Page 416 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database ICP-MS Data Analysis Window 2 Select Data File>>Load. The Load Data File dialog box appears. It allows the user to select the data file to be loaded into data analysis for processing.
  • Page 417 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database N O T E Recently opened data files are also selected from the Data File list box in the ICP-MS Data Analysis window. 3 Select a file using one of the following methods: •...
  • Page 418 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database 5 Select the items in the left box and double click it or click Add-> button. To add Raw Counts/CPS of Quant Element (Replicate Data), click on Raw Counts/CPS and select a numerical value in the each list box for Tune Step, Current Replicate, and Max Replicate Output.
  • Page 419 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database • All Elements tree in the right box After clicking All Elements in the right box, you can select the items under the Quant Element and Quant Element (Replicate Data) tree in the left box and add to the All Elements tree in the right box.
  • Page 420 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database When View>>Edit Box (or click Displays Edit box button on the tool bar) is selected, the Edit Box dialog box appears. You can drag and drop the desired items from the Edit box to the desired position on the Custom Report Template.

  • Page 421: Adding A Chart To A Custom Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Adding a Chart to a Custom Report You can add a chart of the current data file to a custom report. To add a spectrum chart, Time chart, Ion Chart, or Calibration curve, complete the following steps: 1 Select View>>Edit Box.
  • Page 422 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database 2 Select Draw Spectrum (or Draw Timechart) under the Graph tree, and drag and drop it to the desired position on the custom report. Or select Draw Ion Chart (or Draw Calibration Curve) under the Graph tree, and drag and drop it to the desired position on the custom report.

  • Page 423: Preview A Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database 4 Click OK. The chart is drawn. To move the chart, press the Ctrl key, click the chart, and click and drag the chart with the mouse. To size the chart, press the Ctrl key, and click one of the dots and drag it to the desired size.

  • Page 424: Saving The Template

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Saving the Template To set the report file as the custom report template, complete the following steps: 1 Select Template>>Save As. The Save As dialog box appears. Save As Dialog Box 2 Type a name for the file and click Save.

  • Page 425: Generating A Custom Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Generating a Custom Report You can generate a custom report using the custom report template you created. Once you set a custom report template for a method, ChemStation automatically generates a custom report if the proper report parameters are set in the method. For information about setting report parameters, see Chapter 5, “Creating a Method”.

  • Page 426: Creating A Custom Report Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Creating a Custom Report Database You create a custom report database in much the same way as you create a custom report. However, the database has a more fixed format and can accommodate data from a series of analyses.
  • Page 427 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database 2 Select Data File>>Load. The Load Data File dialog box appears. It allows the user to select the data file to be loaded into data analysis for processing. Load Data File Dialog box...
  • Page 428 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Report Wizard 5 Select the Database in the Custom Report box. Report Wizard (Database is selected) 11-16...
  • Page 429 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database 6 Select the items in the left box, and double click it or click Add-> button. The selected items appear in the right box. To add Raw Counts/CPS of Quant Element (Replicate Data), click on Raw Counts/CPS and select a numerical value in the each list box for Tune Step, Current Replicate, and Max Replicate Output.
  • Page 430 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database • All Elements tree in the right box After clicking All Elements in the right box, you can select the items under the Quant Element and Quant Element (Replicate Data) tree in the left box and add to the All Elements tree in the right box.
  • Page 431 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Current Replicate (a number for Current Replicate can be entered directly). Select Current Element and then drag and drop Raw Counts/CPS onto the template. Of the raw data on the element selected in Current Element, only the replicate data set in Current Replicate will be displayed.

  • Page 432: Saving The Database As A Template

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Saving the Database as a Template Before ChemStation can automatically update the database you created, you must first set the database as a template. To do so, complete the following steps: 1 Select Template>>Save As.

  • Page 433: Updating A Custom Report Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Updating a Custom Report Database ChemStation enables you to update the custom report database once the database template is created. Once you link a custom database template to a method and set the method to update the database, your database can be updated automatically when running a method or a sequence.

  • Page 434: Viewing The Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Viewing the Database ChemStation enables you to open a database after you have updated it. You can then use features available in the database menu to create graphs and charts from the database.

  • Page 435: Creating Charts From A Custom Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Creating Charts from a Custom Database ChemStation enables you to create charts from data contained in the custom data- base. This section describes how to create a control chart. To create a chart, complete the steps outlined in “Viewing the Database” in this chapter, then complete the following steps: 1 Select .FQD file and select Template>>Create Database Chart.
  • Page 436 Agilent 7500 ICP-MS ChemStation Operator’s Manual Creating Custom Reports/Database Individual Chart Options Dialog Box 3 Click Chart Options in the View Chart dialog box. The Global Chart Options dialog box appears. The Global Chart Options dialog box allows you to manipulate how the chart will look. You can select the scaling for the chart, select control lines, and select standard deviation lines for the chart.
  • Page 437 Performing a Quantitative Analysis...

  • Page 438: Performing A Quantitative Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Performing a Quantitative Analysis Quantitative analysis enables you to determine the concentrations of specific elements in unknown samples. You run the sample solutions and then calculate concentrations for the elements of interest by comparing them to calibration plots for those elements (external calibration).

  • Page 439: Running Calibration Standards

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Running Calibration Standards To perform a quantitative analysis you must first prepare and analyze calibration standards. Usually you will run a blank solution and then a series of standard solutions containing known concentrations of the analytes. Each calibration standard contains a different concentration of the analyte, and it is typical to run four calibration standards, including the blank or 0 standard.

  • Page 440: Generating A Calibration Curve

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Generating a Calibration Curve ChemStation uses calibration curves to calculate analyte concentrations in unknown samples. You set up calibration curves using the data files ChemStation generated after analyzing calibration standards.

  • Page 441: Creating A New Calibration File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Creating a New Calibration File The following procedure will help in creating a new calibration file. A calibration file can be created in either Online or Offline Data Analysis. The following example is based on Online Data Analysis.

  • Page 442: Selecting A Data File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis External Calibration is the most common method of performing quantitative analysis. To load an element list from the current method, select the Load Masses from Current Acq. Params check box.

  • Page 443: Setting Concentration Levels

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis N O T E Up to 20 levels can be set in a data file. 4 Select the location of the data file using Directory: in the upper left section.
  • Page 444 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 1 Select the Table tab on the Edit Calibration dialog box. Table Tab The Analyte table shows the tune step, mass, and element symbol of each element for which a calibration file has been created. This table allows the setting of the curve fit, ISTD, weight, minimum concentration, units, and concentration values of levels.
  • Page 445 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 4 If the concentration value entered for a level is used to calculate the concentration values for other levels using multipliers, set multipliers using the following method. 1) Select the field of the level to which a concentration value has been entered and click the right mouse button and select Conc Multiply.
  • Page 446 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis For standard addition: • Y=aX+[blank]+bkg • Y=aX+b+bkg. Normally, select this equation. • Excluded 6 To weight the equation, make the following settings. 1) Select an equation from the Weighting Method drop-down list in the upper right section of the Edit Calibration dialog box.
  • Page 447 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Level 2 Level 3 Weighted calibration curve Unweithted Level 1 curve calibration 7 Click on the Units field and select the unit to be used for concentration. 8 Click on the Min Conc field and enter a minimum concentration value.

  • Page 448: Setting The Internal Standard Correction

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis N O T E To save edited calibration information, the calibration must be saved. For online data analyses, select Top window >> Method >> Save Calibration. For offline data analyses, select Offline Data Analysis window >> Method >> Save Calibration.
  • Page 449 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 2 Select the Table tab. Table Tab 3 Enter the tune step, mass, and element symbol to be used as an internal standard in the Add Element area, select the ISTD radio button, and click Add.
  • Page 450 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Configure Analyte/ISTD Dialog Box 5 Click and select the field of the level in which a concentration level is to be entered. Two or more consecutive fields may be selected by clicking on a field and dragging the cursor in a vertical direction.
  • Page 451 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis The Conc Multiply dialog box will appear. Conc Multiply Dialog Box The multiplier for the level input with a concentration value will show 1.00 and the indication will be grayed out (the value cannot be changed).

  • Page 452: Virtual Internal Standard Correction

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Virtual Internal Standard correction 1 Definition of Virtual Internal Standard The latest revision of ICP-MS ChemStation includes a function that allows the internal standard correction factor that is applied to a given analyte to be calculated from the actual signal change of one or more of the internal standards, based on the relative mass of the internal standards and the analyte.
  • Page 453 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 2-2. Effective Example Drift Change & Matrix Effect Dependence on Mass Mass / amu In this scatter plot Li6, Sc45, Y89, Rh103, In115, Tb159 and Bi209 show a clear mass dependency. In such a case this virtual ISTD correction option is useful, in particular some ISTDs are not available.

  • Page 454: Confirming The Graph Summary

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis VIS correction formula Point-to-Point Linear interpolation (no extrapolation) One or more VIS applied to data of two VIS elements elements required Linear Linear formula obtained based on data Two or more VIS...
  • Page 455 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Clicking on a calibration curve will switch to the Graph Detail tab and detailed information on the selected calibration curve will be displayed. 2 The following procedure enables production of text file output of the tune step, mass, correlation coefficient factor (r), DL, BEC (background-equivalent concentration), and unit for each element.

  • Page 456: Confirming The Graph Detail

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 3) Select the location to save the data, enter a name for the text file, and click Save. The data will be saved in a text file. The ICP MS Report Pad window will display the saved text file.
  • Page 457 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 1 Select the Graph Detail tab in the Edit Calibration dialog box. The Graph Detail dialog box will appear. Graph Detail Dialog Box The left side of the dialog box shows the calibration information and a calibration curve.
  • Page 458 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 3 Change the calibration settings as necessary. The set values are also reflected in the Table tab screen. • Changing the element displayed on the graph Select from the Step Mass Element drop-down list. If an internal standard is selected, the Weight check box will change to the VIS Conc check box.

  • Page 459: Printing Calibration Data

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Printing Calibration Data Calibration data can be printed out using the following method. 1 Click Print in the lower left section of the Edit Calibration dialog box. Edit Calibration Dialog Box The Calibration - Print dialog box appears.
  • Page 460 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 2 To output the header information (title, standard data file name, etc.) and the data file information, select the Header Information and Standard Files check box. Calibration - Print Dialog Box 3 To print the content of the Table tab screen, select the Table check box.

  • Page 461: Converting To Calibration Method

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis 2) Select the number of elements to be printed on one page using the #Elements/Page radio button. When “6” is selected, the calibration concentration levels, counts, and RSD will not be printed.

  • Page 462: Blank Subtraction

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Blank Subtraction ChemStation enables you to subtract a blank data file from the sample data file, for example, where the sample reagent blank is different from the standard reagent blank. This blank subtraction is performed after both the blank data file and the sample data file are quantitated (unlike the background subtraction where the background counts are subtracted from the sample counts).

  • Page 463: Generating A Quantitation Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Generating a Quantitation Report ChemStation generates a report showing the results of a quantitative analysis in either of two ways: • You can tell ChemStation to generate the report automatically each time a method runs.
  • Page 464 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis The three most recently loaded data files are listed as numbered menu items, with the first entry corresponding to the most recently loaded data file. Selecting any one of these numbered menu items will cause that file to be loaded into data analysis.
  • Page 465 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Quantitation Report - Summary 12-29...
  • Page 466 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Quantitative Analysis Quantitation Report - Detailed (Text Only) N O T E In the report, the concentration of the internal standard is placed in < > in the specification of the concentration of the element.
  • Page 467 Performing a Semiquantitative Analysis...

  • Page 468: Performing A Semiquantitative Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Performing a Semiquantitative Analysis You perform a semiquantitative analysis to obtain information about the concentrations of all elements that are present in an unknown sample. It is a very powerful tool to obtain concentration information for a large number of elements (>70) without the need for external calibration.

  • Page 469: Running A Calibration Sample

    The higher the response value, the more efficiently that element is ionized in the plasma, which gives higher sensitivity for a given concentration. These original response factors have been empirically determined by Agilent Technologies. After you run the calibration standard and edit the existing semiquantitative parameters, ChemStation adjusts the original response factors to give “corrected factors”, which...

  • Page 470: Loading A Data File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Loading a Data File Before you can view responses from an unknown sample, you must first load the data file ChemStation generates during data acquisition. To do so, complete the fol- lowing steps: >>...
  • Page 471 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis 4 Select a data file using one of the following methods: • Click a file in the displayed list and click OK. • Double-click a file in the displayed list.

  • Page 472: Entering Concentration Values

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Entering Concentration Values After you load the data file, you must enter the concentrations for the calibration elements before you can view responses for the other elements. To enter concentrations for the calibration elements, complete the following steps: >>...

  • Page 473: Entering Minimum Peak Value

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis 3 Type the concentration for the selected element and click Enter. The concentration value appears in the Conc field for the selected element. 4 Repeat these steps for each element in the calibration solution.

  • Page 474: Selecting Concentration Units For The Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Selecting Concentration Units for the Report ChemStation will select a suitable concentration unit for each element being reported, or you can choose the concentration unit for all elements being reported.

  • Page 475: Entering And Deleting Counts Per Second (Cps) Values

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Entering and Deleting Counts Per Second (cps) Values Although you would normally use the units obtained from the calibration standard to set the corrected responses, there may be times when you want to enter the counts per second (cps) values manually.

  • Page 476: Changing The Original Response Values

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Changing the Original Response Values In normal circumstances, original response values should not be changed. However, there may be situations when you want to change the original values; for example, for research purposes or following significant hardware changes.

  • Page 477: Changing The Mass To Be Analyzed

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Changing the Mass to be Analyzed In normal circumstances, the default masses displayed in the SemiQuant Parameters window are the best choices, so there is no need to change them. However, there may be cases when you want to change the default mass;...

  • Page 478: Correcting Responses For All Elements

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Correcting Responses for All Elements After you have loaded the data file and entered concentration values for the calibration elements, you can view the responses for all other elements. N O T E The procedures in the following 8 sections assume that you are in the Edit SemiQuant Parameters dialog box.

  • Page 479: Loading Factors From A Calibration File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Loading Factors from a Calibration File ChemStation enables you to load semiquantitative factors from a calibration file. 1 Click Load Factors from Calib. The SemiQuant Parameters dialog box will appear.

  • Page 480: Viewing The Response Graph

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Viewing the Response Graph After you correct responses for all elements, you can view a graph of those responses. To do so, complete the following steps: 1 Click Response Graph.

  • Page 481: Specifying An Element For Report Output

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Specifying an Element for Report Output In semiquantitative analysis, it is possible to control the inclusion of individual elements in the report. For instance, elements receiving interference from oxides, doubly charged ions, etc can be excluded from the report.

  • Page 482: Loading Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis 2 Enter a file name and click OK. The Save SemiQuant Parameter File dialog box will close and the semiquantitative parameters will be saved. Loading Parameters The saved semiquantitative parameters can be loaded into ChemStation using the following method.

  • Page 483: Using Internal Standard Correction

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Using Internal Standard Correction ChemStation enables you to make internal standard corrections if you add internal standards to your sample in known concentrations. In semiquantitative analysis, internal standard correction is strongly recommended, since changes in sample matrix can effect instrument sensitivity, which will directly affect the reported concentration values.
  • Page 484 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis N O T E To use the Internal Standard Correction manually, a data file to be used as the “Internal Standard Data File” should be acquired prior to setting up the Internal Standard Correction.
  • Page 485 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Select Data File Dialog Box 5 Select the Internal Standard Data File previously acquired using one of the following methods: • Click the file in the displayed list and click OK.
  • Page 486 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis 6 Click the arrow under Elements in the Internal Standard Elements box. All elements are in this list. 7 Select the internal standard element from the list. The mass to be used as the internal standard will automatically be selected from the SemiQuant Parameters.

  • Page 487: Normal Mode

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Normal Mode The Normal Mode is used when the ISTD is added to the unknown sample only. This mode can also be used with Laser Ablation, where the matrix element is used as the ISTD.
  • Page 488 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis N O T E To set the Background File and Interference Correction, select Method >> Data Correction in the ICP-MS Data Analysis window. 4 Click the arrow under Element in the Internal Standard Elements box.

  • Page 489: Subtracting The Blank Data File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Subtracting the Blank Data File ChemStation enables you to subtract a blank data file from the sample data file. This blank subtraction is performed after semi-quantitation is performed on both the blank data file and the sample data file (unlike background subtraction where the background counts are subtracted from the sample counts).

  • Page 490: Generating A Semiquantitation Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis Generating a Semiquantitation Report ChemStation generates a report showing the results of a semiquantitative analysis in either of two ways. • You can tell ChemStation to generate the report automatically each time a method runs.
  • Page 491 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis 4 Select the destination for the report. You can click one or more of the following check boxes: • Screen Generates the report to the ChemStation screen. • Printer Generates the report to the ChemStation printer. You must select this destination for a detailed report.
  • Page 492 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing a Semiquantitative Analysis 13-26...
  • Page 493 Performing an Isotope Ratio Analysis...

  • Page 494: Performing An Isotope Ratio Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis Performing an Isotope Ratio Analysis The Isotope Ratio Analysis function enables the operator to measure and quantitate isotope ratios using the Agilent 7500. It is mainly used for geological and nuclear applications such as determining the age and origin of geological samples, and mea- suring uranium enrichment.

  • Page 495: Setting Isotope Ratio Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis Setting Isotope Ratio Parameters To generate a report for isotope ratio analysis, you must first set the parameters from the ICP-MS Data Analysis window. Setting isotope ratio parameters includes setting mass bias correction, selecting the element, and specifying the isotope ratio.
  • Page 496 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis ICP-MS Data Analysis Window 2 Select Isotope>>Edit IR Parameters. The IR Parameters dialog box appears. IR Parameters Dialog Box 14-4...
  • Page 497 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis 3 Click the Bias Correction check box to make mass bias correction. The Bias Correction check box will be checked. N O T E ICP-MS does not have the exact same sensitivity for all masses. Variations in mass response (mass bias) occur in the interface, ion lenses, analyzer and detection systems.
  • Page 498 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis When data is acquired in the Multi Tune mode, select the tune step in Tune Step to use for the data analysis. When the Multi Tune mode is not used, select “1” for the tune step.
  • Page 499 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis 8 Fill in the IR Output Format panel. To do so, complete the following steps: a) Click on a cell under the Numerator. The cell will be high-lighted and a Numerator text box with a drop down list and an Enter button appears.

  • Page 500: Generating An Isotope Ratio Analysis Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis Generating an Isotope Ratio Analysis Report You can tell ChemStation to generate the report automatically each time a method runs. When you do so, you specify whether to generate an Isotope Ratio Report or a Count Report (see below), and whether to send the report to the screen, the printer or a file.
  • Page 501 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis Load Data File Dialog Box • Sort By Allows the user to sort the list of data files in the current directory by the selected type of information. Options include File Name, Sample Name, Misc Info, and Acquired Date.
  • Page 502 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis and soil03.d is currently displayed, selecting Next Data File will load water01.d. If the current data file is the last one in the directory (in this example water03.d) the message No Next File is displayed.
  • Page 503 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis • Screen Generates the report and displays it on the ChemStation screen. • Printer Sends the report to the ChemStation printer. • File Generates the report and saves it as a file. Use the dialog box to give this file a name.
  • Page 504 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Ratio Analysis Isotope Ratio Report - Count (CPS) 14-12...
  • Page 505 Performing an Isotope Dilution Analysis...

  • Page 506: Performing An Isotope Dilution Analysis

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis Performing an Isotope Dilution Analysis Isotope dilution (ID) is an analytical technique used when the highest accuracy is demanded. Typical applications include the certification of standards and reference materials. ID is highly accurate since it uses known isotopic ratios to quantify analyte concentration.

  • Page 507: Setting Isotopic Dilution Analysis Parameters

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis Setting Isotopic Dilution Analysis Parameters To perform isotope dilution analysis, you must first enter the ID parameters via the ICP-MS Data Analysis window. Isotope dilution analysis parameters to be set include mass bias correction and spike information.
  • Page 508 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis ICP-MS Data Analysis Window 2 Select Isotope>>Edit ID Parameters. The ID Parameters dialog box appears. ID Parameters Dialog Box 3 Click the Bias Correction check box to make mass bias correction.
  • Page 509 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis N O T E ICP-MS does not have the exact same sensitivity for all masses. Variations in mass response (mass bias) occur in the interface, ion lenses, analyzer and detection systems.
  • Page 510 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis C A U T I O N Make sure the tune step used for data acquisition is the same as that selected here. Otherwise, an error may occur. • Background File The name of the background file specified for count correction appears.
  • Page 511 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis Select Element Dialog Box When an element is selected, the Edit Element dialog box appears. Edit Element Dialog Box 15-7...
  • Page 512 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis The Edit Element dialog box displays the masses and isotope abundances of the element selected in the Select Element dialog box. To output the results of the Isotope Dilution analysis, enter the following items.

  • Page 513: Generating An Isotope Dilution Analysis Report

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis Generating an Isotope Dilution Analysis Report You can tell ChemStation to generate the report automatically each time a method runs. When you do so, you specify whether to generate an isotope dilution analysis report, and whether to send the report to the screen, the printer or a file.
  • Page 514 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis Load Data File Dialog Box • Sort By Allows the user to sort the list of data files in the current directory by the selected type of information. Options include File Name, Sample Name, Misc Info, and Acquired Date.
  • Page 515 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis and soil03.d is currently displayed, selecting Next Data File will load water01.d. If the current data file is the last one in the directory (in this example water03.d) the message No Next File is displayed.
  • Page 516 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis • Detailed, Text Only Reports parameters used in the Isotope Dilution method in addition to the ana- lyte value and counts. 7 Select the destination for the report. You can click one or more of the following check boxes: •...
  • Page 517 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis Isotope Dilution Report - Detailed (Text Only) 15-13...
  • Page 518 Agilent 7500 ICP-MS ChemStation Operator’s Manual Performing an Isotope Dilution Analysis 15-14...
  • Page 519 Tools Menu...

  • Page 520: Tools Menu

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tools Menu Tools Menu ChemStation provides the tool to generate multiple types of reports for multiple data files continuously. ChemStation also enables you to select report types and destinations for the data files you want to report. It is convenient to generate reports for many data files at once, after you have finished acquiring all data.
  • Page 521 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tools Menu Select a Report Option Dialog Box 4 Select the report options you want to generate. Select at least one report option. If you do not select a report option, this dialog box closes as you click Go, and no report is generated.
  • Page 522 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tools Menu 6 Move the data files to the right list box by selecting the data files and then clicking Add. • If you wish to delete data files from the right list box, click Remove after you select the files in the right list box.

  • Page 523: Configure Dolist

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Tools Menu Configure DoList Configure DoList enables you to select the report options for DoList. There are many available report options including report type and destination, printing custom reports and updating custom databases, and running user-specified macros.
  • Page 524 Agilent 7500 ICP-MS ChemStation Operator’s Manual Tools Menu The available report options are displayed in the Options List. These options include report type and destination, as well as the ability to generate custom reports, update custom databases, and run user-specified macros.
  • Page 525 DataBase Editor...

  • Page 526: Database Editor

    Agilent 7500 ICP-MS ChemStation Operator’s Manual DataBase Editor DataBase Editor This chapter explains how to edit the ICP-MS databases which are supplied with the ChemStation. Starting the DataBase Click Start button on the Windows Task bar and select Program>>ICP-MS ChemStation>>DataBase Editor.

  • Page 527: Select Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual DataBase Editor Select Database When the database editor is started, the Select database dialog box appears. Several databases are displayed. Select database Dialog Box • Edit Press this button to edit an existing database. Select a database and press Edit.

  • Page 528: Editing The Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual DataBase Editor Editing the Database Index Dialog Box • Edit Pressing Edit after selecting an element or polyatomic molecule brings up the Molecular Database Editor. The atomic numbers of items that have been edited, cut, or pasted are preceded by an asterisk (*).

  • Page 529: Using The Molecular Database Editor

    Agilent 7500 ICP-MS ChemStation Operator’s Manual DataBase Editor Using the Molecular Database Editor You can enter or change information such as description of elements or polyatomic molecules in various databases, using the Molecular Database Editor. Molecular Database Editor • OK Sets the contents displayed on the screen.

  • Page 530: Save Database

    Agilent 7500 ICP-MS ChemStation Operator’s Manual DataBase Editor Save Database Save Database Dialog Box • OK Select an existing database from the list or enter a new database name and click OK to update the database. • Cancel Cancels saving the database.
  • Page 531 Installing the Agilent 7500 ChemStation Software (Windows XP)

  • Page 532: Installing The Agilent 7500 Chemstation Software (Windows Xp)

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Installing the Agilent 7500 ChemStation Software (Windows XP) This section explains how to set up the computer and install the ChemStation software G1834B (Rev. B.02.00 or later) for Windows XP.

  • Page 533: Windows Xp Configuration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Windows XP Configuration 1 Turn on the computer and check the following BIOS settings. • Power setting: Disabled (for example: Auto Suspend Timeout, Hard Drive Timeout, Modem Ring) •...
  • Page 534 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 2 Start Windows XP. The first time Windows XP is started, the setup wizard appears. Follow the instructions. When you have completed the wizard steps, the computer will automatically restart.
  • Page 535 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Select the Start Menu tab, and select the Start Menu. Click OK. 6 Folder Options Right-click Start and open Explore. Select Tools>>Folder Options. Select the View tab and confirm the following settings:...
  • Page 536 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Select the Date tab and select MM/dd/yyyy in the Short Date Style box. Select the Time tab and select HH:mm:ss. 18-6...
  • Page 537 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Click on the Number tab and Select “.” (Dot) in the Decimal Symbol box. Click OK. 8 Logon to Support, Chemist, and User. And set the same setting of step 4 through step 7.
  • Page 538 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 6 Click Advanced button, select WINS tab, then select ON the Enable LMHOSTS lookup. 7 Click OK. 18-8...

  • Page 539: Creating User Account

    You should also be familiar with the particular hardware devices and configurations of your organization's local area network. Agilent Technologies is not responsible for any software problems, LAN configuration conflicts or performance problems that may result when a system is connected to a non-isolated LAN.
  • Page 540 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 4 Right-click the user name, then select Set Password and set the password for each user name. N O T E To create a new user account, select Action>>New User menu in the Computer Management.

  • Page 541: I/O Library Setup

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) I/O Library Setup 1 Insert Agilent ICP-MS ChemStation System disk into CD-ROM Drive. Internet Explorer automatically appears. Click SICL Installation. 2 Click Open. 18-11...
  • Page 542 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 3 InstallShield Wizard Click Next or Yes 18-12...
  • Page 543 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 4 Select Installation Option Select Full Installation. Then Click Next 5 Current Setting Click Next Installation status is shown. Wait until installation has completed. 6 Installation Completion When the installation is complete, the following dialog box will appear.
  • Page 544 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Select [RUN IO Config]. And Click Finish. 7 IO Config Following dialog box will appear. Click [Auto Config]. Following message will appear. Click OK. 18-14...

  • Page 545: Bootp Service Setup

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) BootP Service Setup When GPIB is used skip this BootP Service Setup. Configure GPIB see page 18-36. 1 Click Agilent BootP Service Installation. 2 Click Open.
  • Page 546 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 3 Installation Wizard will appears. Click Next or Yes. Installation status is shown. Wait until installation has completed. 4 The Bootp Settings dialog box appears. 18-16...
  • Page 547 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 5 Select Maintain bootp tabfile?, enter the value of the Subnet mask, then click Launch Manager. Subnet mask:255.255.255.0 Keep the displayed location of the Bootptab File Location.
  • Page 548 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) The value specific to your ICP-MS. N O T E After installing the ChemStation software, you must set the same IP address in the ICP-MS Configuration Window as set in the Bootp configuration window.
  • Page 549 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 4 Click OK. 5 Reboot the computer. N O T E If the PC and the ICP-MS is connected to site LAN, disconnect the cabel from site LAN.
  • Page 550 You should also be familiar with the particular hardware devices and configurations of your organization's local area network. Agilent Technologies is not responsible for any software problems, LAN configuration conflicts or performance problems that may result when a system is connected to a non-isolated LAN.

  • Page 551: Printer Settings

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Printer Settings This section describes the printer setting. The printer setting will execute automatically, when you connect the printer and the computer with the cable and turn on the power. If the printer setting does not execute automatically, complete the following steps to setup the printer.
  • Page 552 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 4 Select Local printer and remove check at Automatically detect and install my Plug and Play printer. Then click Next. 5 Depending on the cable attached to the printer, the selection of Printer Port is variable.
  • Page 553 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 6 Select the printer name and click Next. 7 Select Yes. 8 Select Do not share this printer. 18-23...
  • Page 554 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 9 Select Yes or No and click Next. If you select Yes, confirm Test Page. 10 Click Finish to complete driver installation. Then printer driver is now installed.

  • Page 555: Installing The Flashplayer

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Installing the FlashPlayer Macromedia Flash Player is necessary to play the movies in the on-line help. 1 Insert Agilent ICP-MS ChemStation System disk into CD-ROM Drive.

  • Page 556: Installing The Agilent 7500 Chemstation Software

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Installing the Agilent 7500 ChemStation Software N O T E Before installing the ICP-MS ChemStation software, the followings must be installed. - Microsoft Windows XP Service Pack 2 - SICL driver (I/O Libraries ver.
  • Page 557 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 1 Insert the ICP-MS ChemStation System disk into the CD-ROM drive. Internet Explorer automatically appears. Click ICP-MS ChemStation Installation. 2 Click Open 18-27...
  • Page 558 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 3 Click Next 4 Registration dialog box appears. Enter the User Name and the Company Name. Input Reg# that is in the registration pack, then click Next.
  • Page 559 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 6 Select the destination folder, then click Next. N O T E If the ChemStaiton is already installed, this screen does not appear. 7 Backup ChemStation Files dialog box appears.
  • Page 560 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 8 Start Copying Files dialog box appears. Click Next. 9 Follow the displayed message, install the ChemStation software and Help files. After the installation, the Option Software dialog box appears.
  • Page 561 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 10 Click OK, then ICP-MS configuration dialog box appears. Check the ICP-MS Configuration and then click the Save button to save the configuration. N O T E The IP address must be the same as set in the Bootp configuration window.
  • Page 562 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 14 The message asking for the Agilent 7500 to be placed in Shutdown mode is displayed. Click OK. N O T E If the instrument is not in shutdown mode, press the shutdown switch on the underside of the right instrument cover.

  • Page 563: Initialization Of A Tune File To The Factory Default Condition

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 20 Start the ICP-MS ChemStation and download the firmware. Double click ICP-MS Top. When the ICP-MS Top window appears, download of the firmware starts automatically. (it takes approximately 10minutes) 21 Go to standby mode.
  • Page 564 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) • 7500a: No tune file is displayed. • 7500ce: Standard, H2 gas, He gas • 7500cs: Standard, cool plasma, H2 gas, He gas 4 Click OK.

  • Page 565: Installation The Patch Files

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Installation the Patch Files Before reinstalling ChemStation, the patch file had been installed, it is necessary to install the Patch file after reinstalling ChemStation. You can update the ChemStation Revision by installing the patch file. You can download the patch file from following URL: http://www.chem.agilent.com/scripts/cag_checkreg.asp...

  • Page 566: Gpib Configuration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) GPIB Configuration When GPIB is used for the communication, set the following. 1 GPIB Setting Highlight (Click) [GPIB0] and Click Edit Set as follow. Then Click OK.

  • Page 567: If No Communication With 7500 When Gpib Is 82350A Or 82350B

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 2 IO Config Setting Click OK If No communication with 7500 when GPIB is 82350A or 82350B If ChemStation can not communicate with the 7500 when the GPIB is 82350A or 82350B, Remove the Setting and reconfigure the GPIB setting manually as follows.
  • Page 568 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 2 Select [GPIB 82350 PCI GPIB Card] in the Available Interface Type and Click Configure. 3 GPIB Setting Highlight (Click) [GPIB0] and Click Edit 18-38...
  • Page 569 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Set the following. Then Click OK. 4 IO Config Setting is now Complete Click OK 18-39...

  • Page 570: Changing Windows Firewall Settings To Run Icp-Ms Chemstation Under Windows Xp Sp2

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Changing Windows Firewall Settings to Run ICP-MS ChemStation Under Windows XP SP2 Introduction This section describes the changes in settings that need to be made to run the ICP-MS ChemStation on PCs running Windows XP SP2.

  • Page 571: Changing The Windows Firewall Configuration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) Changing the Windows Firewall Configuration As described in the following steps, enable communications for programs icp- acq.exe and icptune.exe and ports 67 and 68. The detailed port settings to be made...
  • Page 572 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 3 Click the General tab, then select On (recommended) and uncheck the Don’t allow exceptions checkbox. 4 Click the Exceptions tab first, then the Add Program….
  • Page 573 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 5 Click Browse…. The Browse dialog box appears. 6 Click My Computer from the list on the left hand side of the Browse dialog box. Select (C:) --> ICPCHEM --> ICPEXE --> icpacq.exe from the list on the right.
  • Page 574 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 7 Confirm “icpacq.exe” is listed in the Add a Program dialog box, then select “icpacq.exe” and click OK. The Windows Firewall dialog box appears. 8 Confirm “icpacq.exe” is added to Program and Services in the Windows Firewall dialog box.
  • Page 575 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 12 Set the following items and click OK. • Name: Type in “Bootp Client 68 TCP.” • Port Number: Type in “68.” • Click to select TCP or UDP. Here, select TCP.
  • Page 576 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows XP) 14 Confirm that all of the following items have been added in the Windows Firewall dialog box. Click OK. • Bootp Client 68 TCP • Bootp Client 68 UDP •...

  • Page 577: Installing The Agilent 7500 Chemstation Software (Windows 2000)

    Installing the Agilent 7500 ChemStation Software (Windows 2000)

  • Page 578: Windows 2000 Configuration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Installing the Agilent 7500 ChemStation Software (Windows 2000) This section explains how to set up the computer and install the ChemStation software G1834B(Rev. B.01.04 or later) for Windows 2000.
  • Page 579 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Windows 2000 Configuration 1 Turn on the computer and check the following BIOS settings. • Power setting: Disabled (for example: Auto Suspend Timeout, Hard Drive Timeout, Modem Ring) •...
  • Page 580 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 2 Start Windows 2000. If the logon dialog box is automatically displayed, go to step 3. If it’s not displayed, it’s necessary to setup the Administrator’s User name. When finished, go to step 3.
  • Page 581 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Select the Date tab and select MM/dd/yyyy in the Short Date Style box. Select the Time tab and select HH:mm:ss. 19-5...
  • Page 582 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Click on the Number tab and Select “.” (Dot) in the Decimal Symbol box. Click OK. 6 Internet Protocol (TCP / IP) Properties 1 Double click Network and Dial-up Connections icon in the Control Panel.
  • Page 583 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Agilent Technologies is not responsible for any software problems, LAN configuration conflicts or performance problems that may result when a system is connected to a non-isolated LAN.

  • Page 584: Creating User Account

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Creating User Account Creating User Account as follows: User Name Description Password Password property Group Support Product Support HPCE Select Password never expires Administrators check box only. Do not select other check boxes.

  • Page 585: I/O Library Setup

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) I/O Library Setup 1 Insert Agilent ICP-MS ChemStation System disk into CD-ROM Drive. Internet Explorer automatically appears. Click SICL Installation. 2 Select [Run this program from its current location], click OK.
  • Page 586 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Click Yes. 2-1 When Internet Explorer is Version 6, the following dialog Box will appear. Click Open 3 InstallShield Wizard Click Next or Yes 19-10...
  • Page 587 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 4 Select Installation Option Select Full Installation. Then Click Next 19-11...
  • Page 588 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 5 Current Setting Click Next Installation status is shown. Wait until installation has completed. 6 Installation Completion When the installation is complete, the following dialog box will appear.
  • Page 589 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 7 IO Config Following dialog box will appear. Click [Auto Config]. Following message will appear. Click OK. 19-13...

  • Page 590: Bootp Service Setup

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) BootP Service Setup When GPIB is used skip this BootP Service Setup. Configure GPIB see page 18-36. 1 Click Agilent BootP Service Installation. 2 Following dialog Box will appear.
  • Page 591 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Click Yes. 2-1When Internet Explorer is Version 6, the following dialog Box will appear. Click Open 3 Installation Wizard will appears. Click Next or Yes. 19-15...
  • Page 592 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Installation status is shown. Wait until installation has completed. 4 The Bootp Settings dialog box appears. 5 Select Maintain bootp tabfile?, enter the value of the Subnet mask, then click Launch Manager.
  • Page 593 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) The BootP Manager appears. 6 Click Add button. 7 Enter the parameters. MAC Address: Enter the MAC Address of the Agilent 7500. Refer to the next step to know the MAC Address.
  • Page 594 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) N O T E The Host Name must be entered. Host name is restricted to the following characters only: alphabet (A-Z), digits (0-9), and hyphen (-).
  • Page 595 You should also be familiar with the particular hardware devices and configurations of your organization's local area network. Agilent Technologies is not responsible for any software problems, LAN configuration conflicts or performance problems that may result when a system is connected to a non-isolated LAN.

  • Page 596: Printer Settings

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Printer Settings N O T E LJ 4000 or 4050 - Use the printer driver included in the ChemStation CD-ROM. N O T E LJ 2200 - Use the printer driver shipped with the printer.
  • Page 597 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 4 Select Local printer and remove check at Automatically detect and install my Plug and Play printer. Then click Next. 5 Depending on the cable attached to the printer, the selection of Printer Port is variable.
  • Page 598 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 6 Click Have disk. The Install From Disk dialog box appears. 7 Insert the ICP-MS ChemStation System CD-ROM and select the CD-ROM drive. Click Browse and select the folder (\Drivers\HPLaserJet4050) in which the appropriate printer driver exists.Then Click OK.
  • Page 599 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 8 Select the printer name and click Next. 9 Select Yes. 10 Select Do not share this printer. 19-23...
  • Page 600 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 11 Select Yes or No and click Next. If you select Yes, confirm Test Page. 12 Click Finish to complete driver installation. Then printer driver is now installed.

  • Page 601: Installing The Flashplayer

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Installing the FlashPlayer Macromedia Flash Player is necessary to play the movies in the on-line help. 1 Insert Agilent ICP-MS ChemStation System disk into CD-ROM Drive.

  • Page 602: Installing The Agilent 7500 Chemstation Software

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Installing the Agilent 7500 ChemStation Software N O T E Before installing the ICP-MS ChemStation software, the followings must be installed. - Microsoft Windows 2000 Service Pack 4 - SICL driver (I/O Libraries ver.
  • Page 603 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 1 Insert the ICP-MS ChemStation System disk into the CD-ROM drive. Internet Explorer automatically appears. Click ICP-MS ChemStation Installation. 2 Select [Run this program from its current location] click OK.
  • Page 604 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Click Yes. 2-1 When Internet Explorer is Version 6, the following dialog Box will appear. Click Open 3 Click Next 19-28...
  • Page 605 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 4 Registration dialog box appears. Enter the User Name and the Company Name. Input Reg# that is in the registration pack, then click Next. 5 Select Hardware type, then click Next.
  • Page 606 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) N O T E If the ChemStaiton is already installed, this screen does not appear. 7 Backup ChemStation Files dialog box appears. N O T E If the ChemStaiton is not installed yet, this screen does not appear.
  • Page 607 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) After the installation, the Option Software dialog box appears. Select the optional software, which is to be added and enter the registration numbers they are in the registration pack if purchased.
  • Page 608 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 11 Insert G1833-60840 Parameter Disk Assy into Floppy disk driver and click OK. 12 Click Yes if you need the “Installation Checkout Method”. 13 The Readme.txt appear. Read the Readme.txt and Exit the Readme.txt.
  • Page 609 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 17 Select Start>>Programs>>ICP-MS ChemStation>>Edit hosts. Enter the IP Address and the Host Name of Agilent 7500 which is setup in the Bootp Service. Save the hosts file.

  • Page 610: Initialization Of A Tune File To The Factory Default Condition

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Initialization of a Tune File to the Factory Default Condition The following procedure allows initialization of a tune file to the factory default condition. 1 Select Top window >> Instrument >> Tuning.

  • Page 611: Installation The Patch Files

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) Installation the Patch Files Before reinstalling ChemStation, the patch file had been installed, it is necessary to install the Patch file after reinstalling ChemStation. You can update the ChemStation Revision by installing the patch file. You can download the patch file from following URL: http://www.chem.agilent.com/scripts/cag_checkreg.asp...

  • Page 612: Gpib Configuration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) GPIB Configuration When GPIB is used for the communication, set the following. 1 GPIB Setting Highlight (Click) [GPIB0] and Click Edit Set as follow. Then Click OK.
  • Page 613 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 2 IO Config Setting Click OK 19-37...

  • Page 614: If No Communication With 7500 When Gpib Is 82350A Or 82350B

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) If No communication with 7500 when GPIB is 82350A or 82350B If ChemStation can not communicate with the 7500 when the GPIB is 82350A or 82350B, Remove the Setting and reconfigure the GPIB setting manually as follows.
  • Page 615 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 3 GPIB Setting Highlight (Click) [GPIB0] and Click Edit Set the following. Then Click OK. 19-39...
  • Page 616 Agilent 7500 ICP-MS ChemStation Operator’s Manual Installing the Agilent 7500 ChemStation Software (Windows 2000) 4 IO Config Setting is now Complete Click OK 19-40...

  • Page 617: Appendix

    Appendix...

  • Page 618: Top Task

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Appendix A This appendix includes the ChemStation menus. It enables you to quickly determine which menu to access for a specific purpose. Top Task [Instrument] [Instrument Control...] [Tune...] [Exit] [AcquireData] [Main Panel...] [Edit AMU Select File (.amu)...] [DataAnalysis] [Main Panel...]...
  • Page 619 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix [Sequence] [Load and Run Sequence...] [Run...] [Reprocess Data Batch…] [Edit Sample Log Table...] [Position and Run...] [Simulate Sequence...] [View Sequence Log...] [Print Sequence Log] [Load...] [Save...] [Print] [More...] [Chain Sequence] [Edit and Run...] [Simulate Chain Seq] [View Chained Seq Log…]...

  • Page 620: Instrument/Tuning Task

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Instrument/Tuning Task Instrument Control [Plasma] [Plasma ON] [Plasma OFF] [Exit] [Vacuum] [Vacuum ON] [Vacuum OFF] [Diagnostics] [View Error Log...] [Clear Error Log] [Running Time...] [Ion Lenses Test] [Nebulizer Test] [Maintenance] [Sample Introduction...] [Restart S/C Control]...

  • Page 621: Tuning

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix [Maintenance Log] [Log Book…] [Help] [Help topics] [About...] Tuning [File] [Load Tune Values...] [Save Tune Values...] [Load Factory Defaults] [Generate Report] [Print] [Copy Tune Parameters...] [Exit] [Tune] [Sensitivity] [Oxide Ion] [Doubly Charged Ion]...
  • Page 622 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix [ALS] [Goto...] [Home] [Rinse] [ALS Initialize] [Meters] [Meter Control Panel...] [Maintenance Log] [Log Book…] [Help] [Help topics] [About...]...

  • Page 623: Data Acquisition Task

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Data Acquisition Task Main Panel [AcquireData] [Acquire Data...] [Exit] [Edit Parameters] [Set Mode...] [Set Parameters...] ---For an operation other than Time Program acquisition [Set Time Program...] [Print Summary...] [PeriPump] [Set Peripump Program...] [ALS] [Goto...]...

  • Page 624: Data Analysis Task

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Data Analysis Task [Data File] [Load...] [Edit Header...] [Display Replicate Files...] [Edit Average File...] [Print...] [Next Data file] [Tabulate Spectrum/Point...] [Tabulate Spectrum/Mass...] [Draw Total Ion Chart] [Extract Ion Chart...] [Tabulate Chart Raw Data to CSV...] [Tabulate Chart CPS Data to CSV...]...
  • Page 625 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix [Calibrate] [Edit Calibration] [Print Blank Conc.] [Load Calibration...] [Save Calibration...] [Convert to Calibration Method] ---in Standard Addition only [FullQuant] [Generate Report...] [Layout Custom Report...] [Print Custom Report...] [Update DataBase] [Integration Parameters...] [Integration Results] [SemiQuant] [Generate Reports...]...
  • Page 626 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix [Tools] [DoList...] [Configure DoList...] [Copy Window...] [Reset Window] [Help topics] [About…] [Help] [Help topics] [About...] A-10...

  • Page 627: Data Base Task

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Data Base Task [DataBase] [Element.db] [Oxide.db] [Dimer.db] [Dcharge.db] [Hydride.db] [Argide.db] [Chloride.db] [Exit] [View] [Element Information] [Interference Information] [AMU Information] [Prev!] [Next!] [Index...] [Help] [About ICP-MS DataBase...] A-11...

  • Page 628: Report Pad

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Report Pad [File] [Open] [Print] [Exit] [Edit] [Copy] [Select All] [Window] [Cascade] [Tile] [Arrange Icon] [Help] [Help Topics] A-12...

  • Page 629: Calibration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Appendix B This appendix includes descriptions and equations for Calibration, Quantitation, SemiQuantitation and Averaging Repetition Files. Calibration Weighted Regression When data with more than one repetition is used to create a calibration curve, weighted regression can be selected.
  • Page 630 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Internal Standard When an internal standard is selected, the count of each data point in the calibration curve is divided by the ratio of the count per concentration of the internal standard of the same level.

  • Page 631: Coefficients Of Calibration Curves

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Coefficients of Calibration Curves Coefficients are calculated as follows. where n : number of points : numbered value of x : measured value of y : weight of each point ( w =1.0 in the case of unweighted regressions) 1 y = ax ∑...
  • Page 632 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 3 y = ax + bx ⎛ ⎞ ⎛ ⎞ ⎛ ⎞ ⎛ ⎞ ∑ ∑ ∑ ∑ ⎜ ⎟ ⎜ ⎟ – ⎜ ⎟ ⎜ ⎟ ⎝ ⎠ ⎝ ⎠ ⎝ ⎠...
  • Page 633 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix where ⎛ ⎞ ∑ ⎜ ⎟ ⎝ ⎠ ⎛ ⎞ ∑ ⎜ ⎟ – ----------------------- - ⎝ ⎠ ∑ ∑ ⎛ ⎞ ∑ ⎜ ⎟ – --------------------------------- - ⎝ ⎠ ⎛ ⎞ ⎛...
  • Page 634 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 5 log (y) = a (log x) + b ⎛ ⎞ ⎛ ⎞ ⎧ ⎫ ∑ ∑ ∑ ⎜ ⎟ ⎜ ⎟ – ⎨ ⎬ ⎝ ⎠ ⎝ ⎠ ⎩ ⎭ ------------------------------------------------------------------------------------------------------------------------ ⎛...

  • Page 635: Quantitation

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Quantitation Internal Standard When an internal standard is selected in the current calibration curve, the count of the target ion of the sample data is divided by the ratio of the count per concentration of the internal standard in the sample data.
  • Page 636 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 1 y = ax 2 y = ax + b − 3 y = ax + bx − + 4 y = ax + bx + c − + − − a c y 5 log y = a (log x) + b ⎛...
  • Page 637 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix DL refers to a concentration equivalent to 3 σ B. The equation for DL varies depending on the calibration formula. If a linear equation such as y = ax + b is used: DL = 3 σ...

  • Page 638: Standard Deviation Of The Concentration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Standard Deviation of the Concentration When a linear regression is used, the standard deviation is calculated as follows. ⎛ ⎞ ∑ ∑ − ⎜ ⎟ ⎝ ⎠ − where : number of sample repeats...

  • Page 639: Interpolation Formulas For Virtual-Internal-Standard Correction

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Interpolation Formulas for Virtual-Internal-Standard Correction These formulas are used to calculate the modification rates for all internal standards used for the latest calibration curve and the current sample data. The concentration level of the current data internal standards uses the value of level 1 of the calibration curve, as with the existing internal-standard correction.
  • Page 640 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix where Mi : Mass number of internal standards n : Number of internal standards (two in the case of “Point to Point”) Ri : Modification rate of the internal-standard CPS between the standard data and sample data...
  • Page 641 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix ∑ ∑ − ∑ ∑ ∑ − MiRi ∑ ∑ ∑ − ∑ ∑ ∑ − ∑ ∑ − Correct the CPS of the element to be quantitatively analyzed using the virtual-internal-standard correction coefficient.

  • Page 642: Semiquantitation

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix SemiQuantitation The counts used in semiquantitation were the counts of the most abundant point of the mass specified in the semiquant parameters panel for a given element. There are some exceptions to the execution of interference check protocol as...
  • Page 643 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Start cps < min. peak Output is ON? Output is ON? (count / bkg count) < 3% Isotope abundance error <= 10% Oxide Check: (count of target / count of (target - 16 amu)) <= 1% Hydride Check: (count of target / count of (target - 1 amu)) <= 1%...

  • Page 644: Averaging Repetition Files

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Averaging Repetition Files When the repetition data files are averaged to create an average results file, the following formulas are used: ∑ --------------- - ∑ ⎛ ⎞ ∑ ∑ ∑ ⎜ ⎟ –...

  • Page 645: Dead Time Calibration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Appendix C This appendix includes descriptions for the dead time calibration. Dead Time Calibration Dead time is the interval following the arrival of an ion at the detector, during which the counting of a pulse takes place. It is determined by the width of the multiple output pulse, electronic pulse widths and circuit recovery times.

  • Page 646: Execution Of Dead Time Calibration

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Execution of Dead Time Calibration This section describes how to execute the Dead Time Calibration by using erbium (Er) solution. Indium (In) is also available for dead time calibration. For executing the Dead Time calibration, follow the next procedure.
  • Page 647 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Select Use Dead Time Calibration. 4 Introduce the 50ppb Er solution. Tune the sensitivity to make the count of the major isotope Er in the range of 50,000 - 1,000,000 count / 0.1 sec.
  • Page 648 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 5 Select Tune >> Dead time calibration.. Select Element for EM Dead time Calibration dialog box is displayed. 6 Select Er[164/166] then click OK.. Select Element for EM Dead time Calibration dialog box 7 Check sensitivity for 50ppb Er Select Check ..
  • Page 649 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 50ppb check. 50ppb finished. A-33...
  • Page 650 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 8 Introduce the 1ppm Er solution. Check sensitivity for 1ppm Er Select Check .. note the increase in sensitivity with this higher concentration. Select OK then Run .. 1ppm Check. A-34...
  • Page 651 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 9 Select OK to save the new dead time. Typical Dead Time 30 - 60 nano seconds Calibration complete A-35...

  • Page 652: Appendix D Compression And Decompression Of Files

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Appendix D Compression and Decompression of Files For troubleshooting purposes, files such as equipment maintenance log, data, method, calibration, and sequence files can be compressed into zip files. Compressed files can be decompressed.
  • Page 653 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix 3 Click Browse to change the destination folder, if necessary. When the Browse For Folder dialog box appears, select a destination folder and click OK . Browse For Folder Dialog Box 4 Select the type of file to be compressed in the File Type list box.

  • Page 654: Decompressing A File

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Decompressing a File 1 Select Top window >> Tool >> Extract Files. The Open dialog box will appear. Open Dialog Box 2 Select the location of the compressed file and click Open. The Browse For Folder dialog box will appear.

  • Page 655: Appendix E Precautions On Using The Icp-Ms Chemstation With The Lc/Gc Chemstation

    Refer to the instruction manual for each instrument for information on making connections. Note that Agilent 7500 ICP-MS or LC/GC Instruments with the GPIB interface cannot be synchronized with the LC/GC ChemStation.

  • Page 656: Precautions On Installing The Lc/Gc Chemstation On A Pc On Which The Icp-Ms Chemstation Is Already Installed

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Precautions on Installing the LC/GC ChemStation on a PC on which the ICP-MS ChemStation is already installed If the optional ICP-MS chromatographic software is installed on the PC, take the following steps to uninstall the chromatographic software and install the LC/GC ChemStation.

  • Page 657: Precautions During Lc/Gc Startup

    Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix Precautions during LC/GC Startup Open the LC/GC after the ICP-MS ChemStation enters Analysis mode. If you open the LC/GC first, initialize the instrument from the LC/GC ChemStation. Refer to the LC/GC ChemStation Operation Manual for initialization directions.
  • Page 658 Agilent 7500 ICP-MS ChemStation Operator’s Manual Appendix A-42...
  • Page 659 The information contained in this document is subject to change without notice. Copyright Agilent Technologies, 2005 All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under copyright laws.

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