Page 1 Agilent 7100 Capillary Electrophoresis System User Manual Agilent Technologies...
Page 2 Use, duplication or disclo- Research Use Only sure of Software is subject to Agilent Tech- nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as Agilent 7100 Capillary Electrophoresis System...
Page 3 This chapter provides information on connecting the CE instrument to the Agilent ChemStation PC. Using the Agilent 7100 Capillary Electrophoresis System This chapter provides information on how to set up the CE instrument for an analysis and explains the basic settings.
Page 4 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Appendix This chapter provides additional information on safety, legal and web content associated with your 7100 CE instrument. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 5: Table Of Contents
Contents Introduction Introduction to the Agilent 7100 Capillary Electrophoresis System Getting Help Where to Find Information What Learning Products are Available? Site Requirements and Specifications Site Requirements Power Consideration Power Cords Bench Space Environment Physical Specifications Performance Specifications Anforderungen an den Aufstellungsort...
Page 6 Especificaciones físicas Especificaciones de rendimiento 設置について電源について電源コード作業台スペース環境条件物理的仕様性能仕様场地要求电源要求电源线工作台位置环境物理规格性能规格 Installing the Instrument Unpacking the Agilent CE Instrument Damaged Packaging Unpacking the CE Instrument Agilent 7100 Capillary Electrophoresis System User Manual...
Page 7 Manual Configuration With Telnet Agilent Bootp Service Location of the Media Access Control (MAC) Address Using the Agilent 7100 Capillary Electrophoresis System System Definition Controlling the Agilent CE Instrument Before You Start the Agilent CE Instrument Starting Up the Agilent CE Instrument...
Page 8 How to Use Your Agilent CE Instrument for Capillary Electrochromatography Preparing the Agilent CE Instrument for High Pressure Use CEC-Specific GUI Explanation Injection Options with CEC Timetable Options with CEC CEC Control Using the CE Diagram Agilent 7100 Capillary Electrophoresis System User Manual...
Page 9 Main System Firmware Updates Diode Array Detector DAD Control Digital to Analog Converter Changing the Fuses for the Power Supplies Installing Drainage Tubing External Water Bath for Tray Cooling (Optional) Analog to Digital Converter Agilent 7100 Capillary Electrophoresis System User Manual...
Page 10 The Replenishment System Will Not Be Used for Some Time Further Action Operations for Replenishment Maintenance Changing the Air Inlet Filter Accessing the Air Filter Changing the Lamp Removing the Existing Lamp Inserting a New Lamp Further Action Cleaning the Instrument Agilent 7100 Capillary Electrophoresis System User Manual...
Page 11 Setting Up a Test Sample Run Method Parameter CE (Default Settings) Method Parameter DAD Chemical and Biological Safety The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Legal Notice Agilent Technologies on Internet Index Agilent 7100 Capillary Electrophoresis System User Manual...
Page 12 Contents Agilent 7100 Capillary Electrophoresis System User Manual...
Page 13: Introduction
Introduction Introduction to the Agilent 7100 Capillary Electrophoresis System Where to Find Information What Learning Products are Available? This chapter gives an introduction to the Agilent 7100 Capillary Electrophoresis System and where to find specific information or help. Agilent Technologies...
Page 14: Introduction To The Agilent 7100 Capillary Electrophoresis System
Introduction to the Agilent 7100 Capillary Electrophoresis System This handbook gives an overview of your Agilent 7100 Capillary Electrophoresis System. It is designed to get you started working with the system. We strongly advise you to make extensive use of the online help when working with the system.
Page 15: Where To Find Information
• Learning the theory of the technique High Performance Capillary Electrophoresis: An Introduction” The CE Partner CD-ROM: “The comprehensive, interactive tool for beginners and advanced users of CE” • Application Agilent web page (http://www.agilent.com), search for Application notes Agilent 7100 Capillary Electrophoresis System User Manual...
Page 16: What Learning Products Are Available
• Installing Your Agilent ChemStation for LC and CE Systems • Installing and Understanding Your Spectra Module • Understanding Your Agilent ChemStation • Online help • Understanding Your Agilent ChemStation for CE Systems Agilent 7100 Capillary Electrophoresis System User Manual...
Page 17: Site Requirements And Specifications
Agilent 7100 Capillary Electrophoresis System User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Anforderungen an den Aufstellungsort Abmessungen und Gewicht Leistungsspezifikationen Exigences d’installation Caractéristiques physiques Caractéristiques de performance Requisiti ambientali Specifiche fisiche Specifiche delle prestazioni Requisitos de las instalaciones Especificaciones físicas...
Page 18: Site Requirements
WA R N I N G Shock hazard or damage to your instrument can result if the device is connected to a line voltage that is higher than specified. • Connect your instrument to the specified line voltage. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 19: Power Cords
• Never operate your instrument from a power outlet without a ground connection. • Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables...
Page 20: Bench Space
(1.0 inch) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections. If the Agilent 7100 Capillary Electrophoresis instrument is to be placed on a bench, make sure that the bench is designed to bear the weight of the system.
Page 21: Physical Specifications
(218 psi) can be used. A respective male adapter that fits into the instrument’s female adapter and PTFE tubing are part of the shipment. The Agilent 7100 Capillary Electrophoresis System is designed to operate in a typical N O T E electromagnetic environment where RF transmitters, such as mobile phones, should not be used in close proximity.
Page 22: Performance Specifications
Site Requirements and Specifications Performance Specifications Performance Specifications Table 3 Performance specifications for the Agilent 7100 Capillary Electrophoresis System Type Specification Comments Safety features Extensive diagnostics, error detection and display, current leak detection; low current limit Liquid leak sensor; safety sensors on door and cover disabling high voltage;...
Page 23: Anforderungen An Den Aufstellungsort
WARNUNG Wenn das Gerät an eine höhere Spannung als spezifiziert angeschlossen wird, kann dies zu Überspannungsschäden oder einer völligen Zerstörung des Geräts führen. • Schließen Sie das Gerät an die angegebene Netzspannung an. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 24: Netzkabel
Land bereitgestellte Kabel. Verwendung nicht im Lieferumfang enthaltener Kabel WARNUNG Die Verwendung von Kabeln, die nicht von Agilent Technologies geliefert wurden, kann zu einer Beschädigung der elektronischen Komponenten oder zu Personenschäden führen. • Verwenden Sie ausschließlich Originalkabel von Agilent Technologies, um eine einwandfreie Funktion und die Einhaltung der Sicherheits- und EML-Bestimmungen zu gewährleisten.
Page 25: Platzbedarf
Seite zusätzlich 2,5 cm Platz und ungefähr 8 cm an der Rückseite für die elektrischen Anschlüsse und für eine ausreichende Luftzirkulation. Wenn das Agilent 7100 Kapillarelektrophorese System auf einem Labortisch aufgestellt werden soll, stellen Sie sicher, dass der Tisch ausreichende Tragfähigkeit für das System aufweist.
Page 26: Abmessungen Und Gewicht
Druckmaximum von 15 bar (218 psi) verwendet werden. Ein entsprechender männlicher Adapter für den weiblichen Adapter des Geräts und PTFE-Schläuche werden mitgeliefert. Das Agilent 7100 Kapillarelektrophorese-System ist auf den Betrieb in einer typischen HINWEIS elektromagnetischen Umgebung ausgelegt, in deren unmittelbarer Nähe keine HF-Sender wie z.
Page 27: Leistungsspezifikationen
Site Requirements and Specifications Leistungsspezifikationen Leistungsspezifikationen Tabelle 5 Leistungsspezifikationen für das Agilent 7100 Kapillarelektrophorese-System Spezifikationen Kommentare Sicherheitsvorkehrungen Umfangreiche Diagnose, Fehlererkennung und -anzeige, Kriechstromerkennung; Grenzwert für zu niedrigen Strom Flüssigkeitslecksensor; Sicherheitssensoren an Tür und Abdeckung zur Trennung der Hochspannung; Probenflaschensensor; Steuerung und Agilent ChemStation unter Windows®...
Page 28: Exigences D'installation
Tension de secteur incorrecte au niveau de l’instrument AVERTISSEMENT Il existe un risque d’électrocution ou de dommages à l’instrument en cas de raccordement à une ligne d’alimentation de tension supérieure à celle spécifiée. • Connectez l’instrument à la tension indiquée. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 29: Câbles D'alimentation
Technologies destiné à votre pays. Utilisation de câbles non fournis AVERTISSEMENT L’utilisation de câbles non fournis par Agilent Technologies pourrait endommager les composants électroniques ou provoquer des blessures. • Pour garantir un bon fonctionnement et le respect des règles de sécurité ou de compatibilité...
Page 30: Encombrement
2,5 cm de part et d’autre et d’environ 8 cm à l’arrière pour assurer la circulation de l’air et le passage des raccordements électriques Si le système d’électrophorèse capillaire Agilent 7100 doit être placé sur une paillasse, assurez-vous que celle-ci est conçue pour supporter le poids du système.
Page 31: Caractéristiques Physiques
être utilisée. Un adaptateur mâle respectif adapté à l’adaptateur femelle de l’instrument et des tuyaux PTFE sont livrés avec l’instrument. Le système d’électrophorèse capillaire Agilent 7100 est conçu pour fonctionner dans un REMARQUE environnement électromagnétique usuel, dans lequel des émetteurs de radiofréquences, tels que les téléphones mobiles, ne doivent pas être utilisés à...
Page 32: Caractéristiques De Performance
Site Requirements and Specifications Caractéristiques de performance Caractéristiques de performance Tableau 7 Caractéristiques de performance du système d’électrophorèse capillaire Agilent 7100 Type Caractéristique Commentaires Fonctions de sécurité Diagnostic étendu, détection et affichage des erreurs, détection du courant de fuite ; limite de courant faible Détecteur de fuites ;...
Page 33: Requisiti Ambientali
Requisiti ambientali Requisiti ambientali Un ambiente adatto è importante per garantire le prestazioni ottimali del Sistema per elettroforesi capillare Agilent 7100. Prima di effettuare l'installazione, controllare attentamente che il luogo scelto per l'installazione soddisfi i requisiti indicati di seguito. Alimentazione Lo strumento può...
Page 34: Cavi Di Alimentazione
Technologies per i singoli paesi. Uso di cavi non forniti ATTENZIONE L'uso di cavi non forniti da Agilent Technologies può provocare danni ai componenti elettronici o lesioni personali. • Utilizzare solo cavi forniti da Agilent Technologies, in modo da assicurare il funzionamento corretto e la conformità...
Page 35: Spazio Necessario
2,5 cm su entrambi i lati e di circa 8 cm nella parte posteriore per la circolazione dell'aria. Se si posiziona sul banco l'intero Sistema per elettroforesi capillare Agilent 7100, assicurarsi che il banco sia in grado di sostenere il peso complessivo del sistema. Lo strumento deve essere usato in posizione verticale.
Page 36: Specifiche Fisiche
15 bar. Insieme al prodotto viene fornito un adattatore maschio che si inserisce nell'adattatore femmina dello strumento e tubi in PTFE. Il Sistema per elettroforesi capillare Agilent 7100 è concepito per operare in un tipico NOTA ambiente elettromagnetico nelle cui vicinanze non devono essere presenti trasmettitori RF, come telefoni cellulari.
Page 37: Specifiche Delle Prestazioni
Site Requirements and Specifications Specifiche delle prestazioni Specifiche delle prestazioni Tavola 9 Specifiche delle prestazioni del Sistema per elettroforesi capillare Agilent 7100 Tipo Specifica Commenti Dispositivi di sicurezza Ampia scelta di funzionalità diagnostiche, identificazione e visualizzazione degli errori, rilevamento di perdite di corrente, limite di...
Page 38: Requisitos De Las Instalaciones
Requisitos de las instalaciones Requisitos de las instalaciones Es importante disponer de un entorno adecuado para lograr un óptimo rendimiento del sistema de electroforesis capilar 7100. Antes de comenzar la instalación, compruebe minuciosamente que el lugar elegido cumple los requisitos citados a continuación.
Page 39: Cables De Alimentación
Technologies diseñado para su región. Uso de cables no suministrados ADVERTENCIA El uso de cables no suministrados por Agilent Technologies puede producir lesiones personales o daños en los componentes electrónicos. • Para asegurar un funcionamiento correcto y el cumplimiento de los reglamentos de seguridad o de compatibilidad electromagnética, no utilice nunca cables distintos...
Page 40: Espacio En El Banco
2,5 cm libres a cada lado y unos 8 cm en la parte posterior para la circulación del aire y las conexiones eléctricas. En caso de colocar el Instrumento de Electroforesis Capilar Agilent 7100 en un banco, confirme que éste pueda soportar el peso del sistema.
Page 41: Especificaciones Físicas
15 bares. A la entrega se suministra también el correspondiente adaptador macho que encaja con el hembra y los tubos de PTFE del instrumento. El sistema de electroforesis capilar Agilent 7100 está diseñado para funcionar en un N O TA entorno electromagnético normal, en cuya proximidad no deben usarse transmisores...
Page 42: Especificaciones De Rendimiento
Site Requirements and Specifications Especificaciones de rendimiento Especificaciones de rendimiento Tabla 11 Especificaciones de rendimiento del sistema de electroforesis capilar Agilent 7100 Tipo Especificaciones Comentarios Características de seguridad Diagnósticos completos, detección y presentación de errores, detección de fugas, límite inferior de corriente.
Page 43: 設置について
に設置する必要があります。設置作業を開始する前に、設置場所が以下の要件を満たしているかどうかを十分に確認してください。電源について本機器の電源は広範囲にわたる入力電圧に対応しており、46 ページ図表 12 に記載された範囲の標準的な入力電圧で使用できます。したがって、装置背面に電圧スイッチはありません。電源コードが差し込まれている限り、電源を切っていても、モジュールは警告部分的に通電しています。モジュールの修理作業時や保守作業時に人身障害に至る恐れがあります。たとえば、カバーが開いていてモジュールが電源に接続されている場合の感電などです。 • 電源コネクタに常にアクセスすることが可能か確認します。 • カバーを開ける前に、装置から電源ケーブルを取り外します。 • 装置背面の電子ボックスが開いているときやサイド・パネルが取り外されているときに、電源ケーブルを装置に接続しないでください。装置に対する不適正な入力電圧警告仕様より高い入力電圧に接続すると、装置が衝撃を受けたり損傷したりすることがあります。 • 使用する装置は、指定された入力電圧に接続してください。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 44: 電源コード
緊急時には、いつでも電源から装置を切り離せるようにする必要があります。 • 装置の電源コネクタに簡単に手が届き、抜くことができるかどうか確認します。 • ケーブルを抜くことができるように、装置電源ソケットの後ろには十分な空間を確保してください。電源コード本装置には各種の電源コードが付属しています。すべての電源コードのメス型側は同一の形です。電源コードのメス型側は、装置背面の電源ケーブルコネクタに差し込みます。電源コードのオス型側はコードによって異なり、各使用国または各地域のコンセントに合わせて設計されています。接地していない場合または指定外の電源コードの使用警告接地していない場合や指定外の電源コードを使用した場合、感電や回路の短絡に至ることがあります。 • 接地していない電源を使用して本装置を動作させないでください。 • また、使用する地域に合わせて設計された電源コード以外は、決して使用しないでください。指定外ケーブルの使用警告アジレントによって供給されたケーブル以外を使用すると、電子部品の損傷や人体に危害を及ぼすことがあります。 • 安全基準または EMC 規格への準拠を保証できるよう、アジレント製以外のケーブルは使用しないでください。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 45: 作業台スペース
ています。空気の循環と電源ケーブル接続のために、本装置の両側に 2.5 cm、背面に約 8 cm の空間が必要です。作業台に Agilent 7100 装置を設置する場合、作業台が装置の重量に耐えるように設計されているか確認します。本装置は必ず垂直に設置してください。環境条件本装置は、46 ページ図表 12 に記載されている周囲温度および相対湿度の仕様の範囲内で動作します。モジュール内の結露注意結露によってシステムの電気回路が損傷することがあります。 • 温度変化によってモジュール内に結露が発生する可能性がある環境条件では、モジュールの保管、輸送、または使用を行わないでください。 • 寒冷な天候下でモジュールが出荷された場合は、結露が発生しないように、オートサンプラを梱包箱に入れたままゆっくり室温まで上げてください。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 46: 物理的仕様
安全規格： IEC、設置クラス II、汚染度 2 室内使用専用 CSA、UL 全材料リサイクル可ハウジング CEC ( キャピラリ電気クロマトグラフィ ) モードまたは CE+p ( キャピラリ電気泳動、高圧使用オプション適用 ) モードでアプリケーションを使用する場合、最大 15 bar のオイルフリーの空気または窒素を外部装置から供給できます。本装置のメス型アダプタおよび PTFE チューブに合わせたオス型アダプタが付属しています。 Agilent 7100 電気泳動システムは、標準的な電磁環境で動作するように設計され注記ているため、近くで携帯電話などの無線送信機を使用しないでください。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 47: 性能仕様
センサコントロールおよび Agilent ChemStation (Windows® XP SP3 データの評価対応 ) 通信 LAN、コントローラ・エリア・ネットワーク 0 ～ 1 V (CAN)、RS-232C、APG リモート : Ready、 Start、Stop、Shut-down の各シグナル。 USB ( 将来使用 )。アナログ出力およびアナログ入力。 GLP 機能アーリーメンテナンス・フィードバック (EMF) 機能、メンテナンスとエラーの電子記録 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 48: 场地要求
Site Requirements and Specifications 场地要求场地要求为了确保达到 7100 毛细管电泳仪的最佳性能，务必选择适合的环境。开始安装之前，请仔细检查所选场地是否满足下列要求。电源要求页的表 14 中所列范围内的任何标准仪器电源适合较宽的电压范围，可承受第 51 线路电压。因此，检测器的背面没有电压选择器。只要未拔掉电源线，即使在关闭部件后，部件仍会部分带电。警告在部件盖打开且部件处于通电状态时，维修或维护部件可能会造成人身伤害（如电击危险）。 • 请确保始终可以拔插电源插头。 • 在打开部件盖之前，请拔掉仪器的电源电缆。 • 当仪器背面的电子盒打开或侧板被拆下时，请勿连接仪器的电源电缆。连接至仪器的线路电压不正确警告如果设备连接到的线路电压超过了规定电压，则会造成电击危险或仪器损坏。 • 请将仪器连接至规定的线路电压。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 49: 电源线
Site Requirements and Specifications 场地要求电源插头不可插拔。小心遇到紧急情况时，必须能够随时断开仪器与电源线的连接。 • 请确保仪器的电源插头易于插入和拔出。 • 仪器电源插座后面要有足够空间，以便拔出电缆。电源线仪器随附有多种电源线。所有电源线的母接头均相同。母接头插入到后部的电源插座中。每根电源线的公接头均不同，是为了与特定国家或地区的墙上插座相匹配而专门设计的。没有接地连接或没有使用规定的电源线警告没有接地连接和没有使用规定的电源线可能导致电击或短路。 • 切勿将仪器连接到没有接地连接的电源插座中。 • 除安捷伦科技为使用地区设计的电源线外，切勿使用其他电源线。使用非随附的电缆警告使用非安捷伦科技提供的电缆会导致电子元件受损或人员受伤。 • 为确保功能正常并符合安全规定或 EMC 规定，切勿使用不是由安捷伦科技提供的电缆。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 50: 工作台位置
场地要求工作台位置就仪器的尺寸和重量而言，仪器几乎可置于任何桌子或实验室工作台上。为了便于空气流通和电路连接，需要在仪器左右两侧各留出 2.5 cm 的空间，并在背面留出大约 8 cm 的空间。如果要将 Agilent 7100 仪器放置在工作台上，请确保此工作台能够承受该系统的重量。仪器必须竖直放置。环境页的表 14 中所规定的环境温度和相对湿度要求。仪器的工作环境应符合第 51 部件内的冷凝小心如发生冷凝，将导致系统电子元件受损。 • 温度波动可能会导致部件内发生冷凝，请不要在这种条件下贮存、运输或使用部件。 • 如果在寒冷天气条件下运输部件，不要马上开箱，应等到部件在运输箱内缓慢升至室温后再开箱，以此避免发生冷凝。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 51: 物理规格
无油空气或氮气 2-12 bar 安全标准：IEC、安装类别 II，污染程度 2。仅限于室内使用 CSA、 UL 所有材料均可回收利用外壳对于在 CEC 模式（毛细管电色谱法）或 CE+p 模式（毛细管电泳模式且可选用较高压力）下的应用，则可以使用可提供最大压力 15 bar 的无油空气或氮气的外部压力设备。随附适合于仪器母接头的相应公接头以及 PTFE 管。 Agilent 7100 毛细管电泳系统可以在典型电磁环境下使用，在这种环境下不应近注意距离使用射频发射器，例如移动电话。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 52: 性能规格
全面诊断、故障检测和显示、漏电检测；电流下限液体泄漏传感器；安全传感器（位于门和盖上，用于禁用高电压）；样品瓶传感器；控制和数据评估在 Windows XP (SP3) 下运行的 Agilent ChemStation 通讯 0 V 至 1 V LAN，控制器局域网 (CAN)， RS-232C， APG 遥控：就绪、启动、停止和关机信号。 USB （将来使用）。模拟输出和输入。 GLP 功能维护信息预报 (EMF)，维护和故障的电子记录 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 53: Installing The Instrument
Agilent 7100 Capillary Electrophoresis System User Manual Installing the Instrument Unpacking the Agilent CE Instrument Installing the Instrument In this chapter you will find information on • how to unpack the Agilent CE instrument • how to set up the Agilent CE instrument...
Page 54: Unpacking The Agilent Ce Instrument
Unpacking the Agilent CE Instrument Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.
Page 55: Delivery Checklist
Unpacking the Agilent CE instrument Delivery Checklist Ensure all parts and materials have been delivered with the instrument. Please report missing or damaged parts to your local Agilent Technologies sales and service office. Accessories The accessories shipped with the Agilent CE instrument are in additional boxes on right and left in the instrument box.
Page 56 Snap caps, polyurethane 100/PK 5181-1512 Cable, remote 5061-3378 CE catalog 5988-6616EN LAN cable (crossover) 5023-0203 Bottle, 500 mL, electrolyte 9300-1748 HPCE-Primer G7100-90001 CE System User’s Guide G7100-90000 Glass filter, solvent inlet 5041-2168 Electrode alignment assy G7100-60107 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 57: Installing The Instrument
54. 2 Connect the cables at the rear of the Agilent CE instrument. 3 Install the bottles in the replenishment system, see “Preparing the Replenishment System if Needed” on page 111. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 58 For a description of remaining items refer to the Hardware section. • For more information on cable connection to 3 party detectors or analog out, please refer to “Hardware Information” on page 171. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 59 Electrical connectors Connectors for liquids and external pressure LAN connector supply Liquid cooling in connector Liquid cooling out connector External pressure connector Drainage Line power Figure 2 Connections on the rear of the instrument Agilent 7100 Capillary Electrophoresis System User Manual...
Page 60 Installing the Instrument Installing the Instrument Agilent 7100 Capillary Electrophoresis System User Manual...
Page 61: Lan Configuration
Agilent 7100 Capillary Electrophoresis System User Manual LAN Configuration What You Have to Do First TCP/IP Parameter Configuration Configuration Switch Initialization Mode Selection Link Configuration Selection Storing the Settings Permanently with Bootp Manual Configuration Agilent Bootp Service Location of the Media Access Control (MAC) Address This chapter provides information on connecting the CE instrument to the Agilent ChemStation PC.
Page 62: What You Have To Do First
Lab Advisor software is for example used for troubleshooting reasons (see “Agilent Lab Advisor Software” on page 169). Please see “Starting Your Computer and the Agilent ChemStation Software” on page 81 for information on configuration of the connection settings of the ChemStation software. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 63: Tcp/Ip Parameter Configuration
TCP/IP parameters after power-on. The parameters may be derived from a Bootp cycle, non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 17 on page 64. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 64: Configuration Switch
The configuration switch can be accessed on the right rear side of the instrument. The 7100 CE is shipped with switches 7 and 8 set to ON. This means that the instrument is set to a fixed default IP: 192.168.254.11 To perform any LAN configuration, SW1 and SW2 must be set to OFF.
Page 65: Initialization Mode Selection
Using Stored Using Default Bootp When the initialization mode "Bootp" is selected, the 7100 CE tries to download the parameters from a Bootp server. The parameters obtained become the active parameters immediately. They are not stored in the non-volatile memory of the 7100 CE. Therefore, the parameters are lost with the next power cycle of the 7100 CE.
Page 66 7100 CE. Then, he selects the initialization mode "Using Stored" and powers on the 7100 CE again. From now on, he is able to establish the TCP/IP connection to the 7100 CE with the parameters obtained in that single Bootp cycle.
Page 67 Table 19 on page 68. A direct connection of a computer and the 7100 instrument is the entry point to change the stored parameters of the instrument. The default parameters of the 7100 instrument are permanently stored and cannot be changed. Using them, this allows you to establish a LAN connection at any time when the init mode “Using Default”...
Page 68 TCP/IP connection using the new parameters. When the 7100 CE instrument is wired to the PC directly (for example using a cross-over cable or a local hub), that is separate from the local area network, the user may simply keep the default parameters to establish the TCP/IP connection.
Page 69: Link Configuration Selection
Link configuration Speed and duplex mode determined by auto-negotiation Manually set to 10 Mbps, half-duplex Manually set to 10 Mbps, full-duplex Manually set to 100 Mbps, half-duplex Manually set to 100 Mbps, full-duplex Agilent 7100 Capillary Electrophoresis System User Manual...
Page 70: Storing The Settings Permanently With Bootp
Stored” mode, see Table 18 on page 65. 9 Power cycle the CE instrument. The CE instrument can be accessed now via LAN without the Agilent Bootp Service, refer to “Agilent Bootp Service” page 75. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 71: Manual Configuration
Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, if the initialization mode selection switches permit this. Figure 7 Manual configuration (principle) Agilent 7100 Capillary Electrophoresis System User Manual...
Page 72: With Telnet
LAN Configuration Manual Configuration With Telnet Whenever a TCP/IP connection to the 7100 CE instrument is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. Please refer to “Using Default” on page 67 for establishing an initial LAN connection.
Page 73 Then press [Enter]; parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 74 If the initialization mode switch is changed now to “Using Stored” mode, the instrument N O T E will take over the stored settings when the instrument is re-booted. In the example above, it would be 134.40.27.99. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 75: Agilent Bootp Service
IP address once it is powered up. Detailed documentation on installation and the usage of the Agilent Bootp Service can be found on your ChemStation installation DVD under \Support\ Manuals\Installation. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 76: Location Of The Media Access Control (Mac) Address
Telnet session as described earlier. Please follow the instructions on Manual Configuration, Using Telnet (“With Telnet” on page 72) and see Figure 11 page 74 for the location of the MAC address as the second line in the LAN status page. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 77: Using The Agilent 7100 Capillary Electrophoresis System
Agilent 7100 Capillary Electrophoresis System User Manual Using the Agilent 7100 Capillary Electrophoresis System System Definition Controlling the Agilent CE Instrument Working with the CE Diagram How to Prepare your Agilent CE Instrument for Analysis Capillary Electrophoresis, CE Capillary Electrophoresis, Plus High Pressure CE+p...
Page 78: System Definition
Using the Agilent 7100 Capillary Electrophoresis System System Definition System Definition After your Agilent 7100 Capillary Electrophoresis System has been properly installed (see “Installing the Instrument” on page 53) you can begin exploring the system. Your Agilent 7100 Capillary Electrophoresis System comprises the CE instrument, the ChemStation and the Lab Advisor/Instrument Utilities software.
Page 79: Controlling The Agilent Ce Instrument
Using the Agilent 7100 Capillary Electrophoresis System Controlling the Agilent CE Instrument Controlling the Agilent CE Instrument Before You Start the Agilent CE Instrument Use the following as a checklist before you start the Agilent CE instrument. Make sure that: •...
Page 80 Using the Agilent 7100 Capillary Electrophoresis System Controlling the Agilent CE Instrument Table 22 Status LED color Color Meaning No color Instrument is ready Green Analysis running, do not use instrument with the exception of manual operation of the tray. Some fields in DAD and CE diagram are shaded gray.
Page 81: Starting Your Computer And The Agilent Chemstation Software
After you have switched on your computer and it has booted up, start the Agilent ChemStation by double clicking the Instrument Online icon in the Agilent ChemStation menu. In order to control the 7100 Capillary Electrophoresis instrument, the ChemStation revision N O T E must be B.04.02 or higher.
Page 82: Starting The Agilent Chemstation Software The First Time
Using the Agilent 7100 Capillary Electrophoresis System Controlling the Agilent CE Instrument Starting the Agilent ChemStation Software the First Time Starting the Agilent ChemStation software the first time will lead to the following message: Figure 13 Configuration at first start of ChemStation Please select Yes.
Page 83 Using the Agilent 7100 Capillary Electrophoresis System Controlling the Agilent CE Instrument Figure 15 Configuration menu Please refer to “LAN Configuration” on page 61 for the default IP address or manual N O T E configuration of an individual IP address.
Page 84: Working With The Ce Diagram
Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram Working with the CE Diagram When you start the Agilent ChemStation, the CE and DAD diagram appears by default as the main screen. The CE diagram displays the Agilent CE instrument showing the current status of the system as a whole.
Page 85: Software Toolbar
Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram Software Toolbar Figure 16 Components of the common software toolbar of the ChemStation. Work with sequences Change the layout to show two online 15 Display of elapsed and overall run...
Page 86: Graphical User Interface Explanation
Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram Graphical User Interface Explanation Components of the Agilent CE instrument in the CE diagram (synonym dashboard) are shown in Figure 17. Each component in the instrument is represented by an icon in the CE diagram or DAD diagram.
Page 87 Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram The current analysis mode is displayed in the top line of the CE diagram. The analysis modes that can be selected under instrument configuration are CE, CE+p, CEC or CE-MS.
Page 88: Menus In The Ce And Dad Diagram
Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram Menus in the CE and DAD Diagram The CE and DAD diagram offers access to directly control several instrument components, as well as access to method parameters. Right-clicking in the...
Page 89 Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram Direct Control of Instrument Components The actions are performed immediately and the changes are reflected in the CE diagram. Such actions include, for example: • Switching the lamp on.
Page 90: System Vialtable
Using the Agilent 7100 Capillary Electrophoresis System Working with the CE Diagram System Vialtable The vialtable is a graphical tool which facilitates the management of vials in the tray. It can be used as an intelligent notepad, which exchanges its information with methods and sequences.
Page 91: How To Prepare Your Agilent Ce Instrument For Analysis
Using the Agilent 7100 Capillary Electrophoresis System How to Prepare your Agilent CE Instrument for Analysis How to Prepare your Agilent CE Instrument for Analysis To prepare the Agilent CE instrument for the analysis you need to do the following tasks: •...
Page 92: Capillary Electrophoresis, Ce
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Capillary Electrophoresis, CE How to Use Your Agilent CE Instrument for Capillary Electrophoresis Separations This chapter introduces method parameters for applications running in CE mode. This mode is the most suitable for applications in the field of capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC) and capillary isoelectric focussing (CIEF).
Page 93 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Figure 20 CE Method Setup Agilent 7100 Capillary Electrophoresis System User Manual...
Page 94 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Figure 21 DAD Method Setup Selecting OK transfers the parameters to the instruments and closes the dialog. Choosing Apply only transfers the current parameter to the instrument. Save the edited method, if appropriate.
Page 95 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE • Method Information • Instrument / Acquisition • Data Analysis • Run Time Checklist The book icon in the software toolbar is a further alternate entry point for method editing. When you click the icon, you can access menu items to set method parameters.
Page 96: Vials
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Vials You can program a method to use certain vials as the inlet or outlet home vial. During electrophoresis, the electrodes and the capillary are in these vials. Vial numbers 1 to 48 or user vials (User 1 to User 10) can be selected. The selection can be done from the pull-down menu or by typing in the number with the keyboard.
Page 97: Cassette Temperature
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Cassette Temperature The cassette temperature can either be set to not be controlled or to a certain setpoint. This setpoint must be within the instrument’s specified range (see “Physical Specifications”...
Page 98: High Voltage System
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE High Voltage System The following parameters for the power supply can be controlled using the CE Method Setup screen in the Agilent ChemStation software: • Enable High Voltage • Voltage •...
Page 99 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Voltage, Current and Power Voltage, current and power are related to each other by the resistance of your capillary/buffer system. You can set values for each of these three parameters.
Page 100: Posttime And Stoptime
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Posttime and Stoptime The stoptime defines the time for your separation, also referred to as the run part of the analysis. It does not include replenishment, preconditioning, injection and postconditioning. After the stoptime has elapsed, the system changes from the run state to postconditioning or postrun (if defined) and then to the ready state.
Page 101: Preconditioning
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE The time for a replenishment step can vary from vial to vial. N O T E When using buffers containing detergent (for example SDS), replenishment may not fulfill your needs. The limit for SDS concentration is 70 mM. Do not exceed this concentration.
Page 102 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Wash Inlet Electrode (New Command to Avoid Carry-over in Critical Applications) During precondition and postconditioning a command for emptying the space between capillary and electrode can be executed. The command is dedicated for applications with aqueous buffers and consists of application of slight vacuum to the inlet vial while lowering the inlet vial.
Page 103: Injection
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Injection You have several choices for CE injection: • Hydrodynamic injection (by pressure or vacuum) • Electrokinetic injection (applying a voltage, current or power) • Using an injection program The injection parameters can be programmed using an expandable table in the right side of the CE Method Setup screen.
Page 104 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Electrokinetic Injection For electrokinetic injection the inlet buffer vial is replaced with the sample vial. A voltage, current or power is applied for a certain time that causes the sample to migrate into the capillary.
Page 105: Timetable
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Using an Injection Program The injection table is used for advanced injection tasks such as: • Injection from different vials, for example ITP. • Multiple injection modes, for example spiking.
Page 106 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Instrument Curves Using a further tab in the CE Method Setup dialog box you can choose to monitor and store the following CE-specific raw data. If selected, they are stored in addition to the detector signals defined: For the CE: •...
Page 107: Detector Parameters
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Detector Parameters The parameters for the detector are set in the DAD tab of the Method Setup dialog used in the Agilent ChemStation (see Figure 20 on page 93). On the left hand-side you can define the following settings: •...
Page 108 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Spectra On the right-hand side of the DAD Method Setup dialog an expandable page for advanced settings is visible. Under Spectrum you can define at which points on a signal, spectra will be taken and saved.
Page 109 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Autobalance Enables you to zero the baseline of the diode-array detector before or after an analysis. You can use the autobalance feature to ensure that the baseline is reset to zero either before the Prerun time has elapsed, or after the Postrun time has elapsed.
Page 110: Switching The Lamp On
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Switching the Lamp On The lamp can be switched on automatically as part of initialization (see “DAD Control” on page 180). To switch the lamp on or off manually: 1 In the DAD diagram click the lamp icon. A menu will offer the option to switch the lamp on or off depending of the current status.
Page 111: Preparing The Replenishment System If Needed
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Preparing the Replenishment System if Needed During multiple analyses, the composition of the buffer can change. One way to refresh the buffer is replenishment. The replenishment system automatically empties and refills the buffer vials in the tray with new buffer from the electrolyte bottle.
Page 112 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Instrument damage by corrosion C A U T I O N Components of the replenishment system can be damaged by aggressive or corrosive buffers, organic solvents or evaporated components from these.
Page 113 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE 4 Take out the electrolyte bottle and unscrew the cap. The electrolyte bottle has a frit and tubing inside. Avoid contaminating the frit (see Figure 24 page 113). Filling the Replenishment Bottles...
Page 114 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE 1 Make sure the buffer is filtered; use a 0.25 µm filter. For buffers with a high viscosity, a larger pore size, for example, 0.45 µm is also suitable. 2 Fill the electrolyte bottle with buffer.
Page 115: Preparing Vials For Buffer And Sample
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Preparing Vials for Buffer and Sample Vial Handling • Only use only recommended vials (see Table 4) when working with the Agilent CE instrument. • It is recommended cleaning the vials prior use (flush them with water and some alcohol, use compressed air to dry them), otherwise particles may enter the capillary (current problems or blocked capillary).
Page 116 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Table 23 Recommended vials and caps for the Agilent CE instrument Item Description Part Number To Be Used For Vial clear glass 2 mL Sample and buffer Pack of 100...
Page 117 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE The recommended maximum liquid level in the vials is 1.8 cm measured from the bottom of the vial (see Figure 25). The minimum level depends on the type of liquid the vial is used for (sample, buffer or waste).
Page 118 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Hydrodynamic Injection When using injection by pressure, the sample level only needs to touch the capillary. The minimum liquid level in the sample vials for this technique is 5 mm for standard and short electrodes. The short electrode filling height should not exceed 6 mm.
Page 119 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Outlet Vials Outlet vials (for example, waste vials) should also be filled with enough liquid to touch the capillaries. Thus, the liquid flow through the capillary does not produce droplets at the capillary end, which can cause pressure. This would influence the injection amount.
Page 120 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE The tray will perform a complete turn every time the tray door is closed in order to read-out the occupied tray positions with an internal vial sensor. Methods will only run if all specified vials are present. The vial sensing procedure might also be delayed if any operation prevents the vial handler temporarily from turning.
Page 121 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Removing the Capillary Cassette from the Agilent CE Instrument Your Agilent CE instrument comes with an empty capillary cassette installed. To remove the capillary cassette from the Agilent CE instrument, you have to do the following steps: 1 Choose the cassette icon in the CE diagram and select Change Cassette…...
Page 122 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE 4 Carefully pull the cassette out of the cassette guide. See Figure 27 page 123. Cassette Figure 26 Position of installed cassette The DAD detector is equipped with a white LED located near the outlet electrode. This light N O T E will assist you when inserting the capillary.
Page 123 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Figure 27 Removing the cassette Agilent 7100 Capillary Electrophoresis System User Manual...
Page 124: Installing A Capillary In The Alignment Interface
• Wear safety glasses when opening the cassette and handling the capillary to protect your eyes and body. Alignment interfaces for the 7100 CE instrument differ from alignment interfaces for earlier N O T E instruments. Interfaces from earlier instruments do not fit into the diode array detector block.
Page 125 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Table 24 Alignment interfaces Sleeve Color Part Number Used For Black G7100-60150 25 µm id capillaries with extended light path Green G7100-60210 50 µm id straight capillaries G7100-60230 50 µm id capillaries with extended light path...
Page 126 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE 3 Put the alignment interface on the insertion tool and press down firmly. Press only on the outer ring to release the spring that secures the capillary in the alignment interface.
Page 127 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Figure 30 Extended light path aligned correctly Figure 31 Extended light path aligned incorrectly Agilent 7100 Capillary Electrophoresis System User Manual...
Page 128: Installing A Capillary In An Empty Capillary Cassette
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Installing a Capillary in an Empty Capillary Cassette This procedure describes how to install a capillary in an empty capillary cassette. If the cassette has already been installed in the Agilent CE instrument, remove it as described above.
Page 129 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Cassette front Cassette back Figure 32 Opening the cassette lock Installing the Capillary 1 Hold the capillary at the alignment interface. 2 Insert the alignment interface into the alignment interface holder on the cassette.
Page 130 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE 5 Start with the inside of the upper pin to prevent the capillary from being squeezed when the cassette is closed. Hook Capillary lock Magnet Hook Alignment interface Capillary insertion tool...
Page 131 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Closing the Capillary Cassette 1 Place the top of the cassette onto the bottom part with an offset of 8 mm (0.3 inch). 2 Hold the bottom part and slide the top part towards the top of the cassette until it clicks into place.
Page 132: Inserting The Cassette
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE Inserting the Cassette Use the Change Cassette task from the CE diagram to access an already installed cassette. N O T E To insert the capillary cassette into the system you have to perform the following steps.
Page 133 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, CE 4 Move the cassette guide to the front. Extracting the cassette from the instrument without lowering the lifts will lead to a higher N O T E contamination of the inner parts of the electrode or pre-puncher. Crystallization or liquid residue of this contaminations might bias future runs.
Page 134 6 Move the cassette guide back again while holding the cassette down. The alignment interface aligns the capillary automatically with the detector. Only alignment interfaces dedicated to the 7100 CE instrument will fit. Interfaces from N O T E different instruments will not fit and will lead to unsatisfactory results.
Page 135: Fraction Collection
MALDI/TOF-MS or protein sequencing. All 7100 CE instruments allow fraction collection. This feature will be fully available in future Agilent ChemStation software revisions. Please check regularly for updates at http://www.agilent.com...
Page 136: Capillary Electrophoresis, Plus High Pressure Ce+P
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Capillary Electrophoresis, Plus High Pressure CE+p The Agilent CE instrument is capable of accepting an external gas pressure in addition to the internal pressure. The external high pressure source can be up to 15 bar (218 psi), and is regulated within the instrument via method settings or with direct instructions from the CE diagram between 2-12 bar.
Page 137 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p In connector Liquid cooling Out connector External pressure connector Drainage Figure 35 External high pressure gas inlet Agilent 7100 Capillary Electrophoresis System User Manual...
Page 138: Outlet Lift Connection To The Tubing System
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Outlet Lift Connection to the Tubing System The outlet lift is not connected to the tubing and valve system of the instrument by default. Please connect the respective PTFE tube provided in your accessories kit.
Page 139 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Damage or contamination of pressure valves. WA R N I N G Make sure tube "8" is connected properly before you apply high pressure to any lift.
Page 140: Preparing The Agilent Ce Instrument For High Pressure Use
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Preparing the Agilent CE Instrument for High Pressure Use 1 Ensure that the gas source is switched off prior to connection. 2 Connect the push-fit connector and gas line to the receptor on the rear of the Agilent CE instrument.
Page 141 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p 6 Enable CE+p mode by selecting the option External Pressure Installed. Figure 38 Select CE mode 7 Confirm your selection by clicking OK. 8 Make sure tube “8” is connected, see “Outlet Lift Connection to the Tubing...
Page 142 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p 10 The activated CE+p mode is displayed in the top line of the CE diagram and a new icon for an external pressure reservoir will appear, see...
Page 143: Operation And Function In Ce+P Mode
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Operation and Function in CE+p Mode By selecting CE+p mode, some additional functions are available for control via the CE diagram, which will assist in the operation of capillary gel electrophoresis (CGE) with highly viscous buffers.
Page 144 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Figure 40 Method setup with CE+p mode activated Agilent 7100 Capillary Electrophoresis System User Manual...
Page 145 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Figure 41 Parameters for high pressure flush Other parameters which can be set in the High pressure flush command menu include: • Applied voltage (kV), current (µA) or power (W) •...
Page 146: Injection Options With Ce+P
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Injection Options with CE+p The installed high pressure supply may also be used for injection purposes using the Timetable option in the Injection field of a method setup.
Page 147: Ce+P Control Using The Ce Diagram
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrophoresis, Plus High Pressure CE+p Timetable controlled changes in the pressure settings are: • OFF: Pressure usage is switched off. • Internal, pressure (-100 mbar to +100 mbar): Can be applied to the inlet vial.
Page 148: Capillary Electrochromatography, Cec
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Capillary Electrochromatography, CEC Capillary electrochromatography (CEC) is a fusion of capillary electrophoresis and capillary chromatography. In CEC, solvent is mobilized through a packed capillary column by electroosmotic flow instead of the hydraulic flow used in liquid chromatography LC.
Page 149 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC In connector Liquid cooling Out connector External pressure connector Drainage Figure 43 External high pressure gas inlet Agilent 7100 Capillary Electrophoresis System User Manual...
Page 150: Preparing The Agilent Ce Instrument For High Pressure Use
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Preparing the Agilent CE Instrument for High Pressure Use 1 Ensure that the gas source is switched off prior to connection. 2 Connect the push-fit connector and gas line to the receptor on the rear of the Agilent CE instrument.
Page 151 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC 6 Enable the CE+p mode by selecting the option External Pressure Installed. Figure 45 Select CE mode (to decide which access to config is used) 7 Confirm your selection by clicking OK.
Page 152 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Damage or contamination of pressure valves. WA R N I N G Make sure tube "8" is connected properly before you apply high pressure to any lift. High pressure may flush liquid into the pressure valves and lead to permanent damage.
Page 153: Cec-Specific Gui Explanation
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC CEC-Specific GUI Explanation When the CEC mode is selected, a new icon in the form of a gas bottle appears on the left right side of the CE diagram next to the tray (see Figure 46 page 152).
Page 154 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Figure 47 CEC setup method screen Agilent 7100 Capillary Electrophoresis System User Manual...
Page 155 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Preconditioning and Postconditioning In order to condition a packed capillary, an applied pressure of approximately 12 bar alone is usually insufficient to move the mobile phase rapidly through the column. Therefore, the applied high pressure may be assisted by the application of an electric field.
Page 156: Injection Options With Cec
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Other parameters which can be set in the High pressure flush command menu include: • Applied voltage (kV), current (uA) or power (W) • Duration (s) • Inlet vial and/or outlet vial...
Page 157: Timetable Options With Cec
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Timetable Options with CEC The installed high pressure supply may also be used in the general timetable of a method setup. The Pressure command is extended by the External pressure...
Page 158: Cec Control Using The Ce Diagram
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC CEC Control Using the CE Diagram High pressure may be applied independently of the method for capillary conditioning or cleaning by clicking the external pressure icon in the CE diagram (see Figure 46 on page 152).
Page 159: Running The Cec Analysis
Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Running the CEC Analysis After setting up the operational parameters, you can initiate the run from the Method & Run Control menu by clicking Run Method or by clicking Start in the CE diagram.
Page 160 Using the Agilent 7100 Capillary Electrophoresis System Capillary Electrochromatography, CEC Agilent 7100 Capillary Electrophoresis System User Manual...
Page 161: Troubleshooting And Diagnostics
Agilent 7100 Capillary Electrophoresis System User Manual Troubleshooting and Diagnostics CE Problem Solving Agilent Lab Advisor Software This chapter gives an overview of troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies...
Page 162: Ce Problem Solving
• Check fuses 1 to 6 on the main electronic board. A blown fuse is indicated by a small red LED visible through a hole in the metal shield of the instrument. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 163: Leaks
• The error LED on the front of the instrument is illuminated. • The CE status displays an error. • A logbook entry is created. • The run is stopped. • Pressure is released. • High voltage application is stopped. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 164 Leaks in Replenishment Tubing or Valves • Call Agilent Technologies. Reason Unknown • If you can not trace the reason for the leak, call Agilent Technologies. There may be an instrument problem. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 165: Blocked Replenishment Needle
• A contaminated insulation plate • A high humidity level • A conductive capillary surface If you find a broken capillary in the alignment interface, use an ultrasonic bath to clean the interface and remove quartz residues. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 166: Broken Capillary
• Exchange the broken capillary Contaminated Insulation Plate Salt crystals or other contamination of the insulation plate may cause leakage current. • Clean the insulation plate as described in “Cleaning the Insulation Plate” page 207. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 167: Empty Injection Vial
Small air bubbles in the capillary may cause spikes in your electropherogram. Larger air bubbles expand so much during analysis that the current drops below the lower alarm limit. • Flush your capillary with buffer to remove air bubbles. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 168: Problems With The Replenishment System And Buffers Containing Sds
2 Program methods which use these vials as the inhome and outhome vials (for example vials 4, 5, 6, 7). Method 1: inhome = 4, outhome = 5 Method 2: inhome = 6, outhome = 7 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 169: Agilent Lab Advisor Software
For details, refer to the Agilent Lab Advisor software help files. Configuration The Lab Advisor software can communicate with a 7100 CE instrument via LAN or by a serial connection (RS232 cable, null-modem). To configure a connection, go to the section Configuration in the Lab Advisor software. Select Instrument >...
Page 170 Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent 7100 Capillary Electrophoresis System User Manual...
Page 171: Hardware Information
Agilent 7100 Capillary Electrophoresis System User Manual Hardware Information Identifying Instrument Components 7100 Capillary Electrophoresis Instrument Electronics Firmware Diode Array Detector Changing the Fuses for the Power Supplies Installing Drainage Tubing External Water Bath for Tray Cooling (Optional) Analog to Digital Converter This chapter provides a more detailed description of the instrument’s...
Page 172: Identifying Instrument Components
Identifying Instrument Components Cassette cooler Cassette Detector Insulation plate Replenishment needle Outlet lift Inlet lift Connector for status board Replenishment lift Sample tray Air pump Electrolyte bottle Waste bottle Figure 52 Overview of instrument components Agilent 7100 Capillary Electrophoresis System User Manual...
Page 173: 7100 Capillary Electrophoresis Instrument Electronics
7100 Capillary Electrophoresis Instrument Electronics 7100 Capillary Electrophoresis Instrument Electronics Fuses LAN connector USB connector Remote control Serial connector Configuration Analog In/Out Line fuses Line power Figure 53 Connectors on the rear of the instrument Agilent 7100 Capillary Electrophoresis System User Manual...
Page 174 • Power connector, Fuse netfilter: 2 fuses, 250 VAC, T6.3AH, (part number 2110-1018) • LAN connector (7100 to controlling PC) • CAN connectors (7100 to additional Agilent 1200 Series Module) • USB connector (for future use) • RS232 connector (serial interface to a computer. This connection has a special out pin and is not compatible for connecting printers or plotters.
Page 175 • For continued protection against fire hazards, only replace fuses with the same type and rating. • Only use the fuses specified for this instrument. Use of other fuses or materials is prohibited. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 176: Firmware
• Run synchronization through APG remote • Error handling, • Diagnostic functions • Or module-specific functions: • Internal events such as lamp control, filter movements • Raw data collection and conversion to absorbance Agilent 7100 Capillary Electrophoresis System User Manual...
Page 177: Firmware Updates
Updates to main system can only be done in the resident system. Updates to the resident N O T E system can only be done in the main system. Main and resident firmware must have the same version. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 178 Hardware Information Firmware Main firmware update Resident system Main system Resident firmware update Figure 54 Firmware update mechanism The latest firmware revisions and documentation are available on the Agilent website (http://www.chem.agilent.com) Agilent 7100 Capillary Electrophoresis System User Manual...
Page 179: Diode Array Detector
Direct control of the DAD and control through method parameter is described in Controlling the Agilent CE instrument (“Detector Parameters” on page 107) and the section “User interface” in the ChemStation online help. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 180: Dad Control
In the Automatic Turn On field you can also set a date and time when the lamps are switched on automatically. The instrument must be kept powered on, for example overnight, to use this feature. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 181: Digital To Analog Converter
Diode Array Detector Digital to Analog Converter The 7100 CE System provides a direct analog output of chromatographic signals A. It may be used to connect to another data acquisition system or chart recorders. The analog signal is converted from digital format by the on-board 20-bit digital-analog-converter (DAC).
Page 182: Changing The Fuses For The Power Supplies
• For continued protection against fire hazard, replace fuses only with the same type and ratings. • Only use the fuses specified for this instrument. The use of other fuses or materials is prohibited. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 183: Installing Drainage Tubing
On the left rear side of your instrument you may install silicone tubing. Place a beaker of at least 200 mL capacity under this tubing to collect the drained condensation. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 184: External Water Bath For Tray Cooling (Optional)
• Requirements for external liquid circulation: Suitable for external circulating of liquid, Suitable for connection of 8 mm ID tubes, Minimum circulating liquid flow rate 8 L/min, Agilent 7100 Capillary Electrophoresis System User Manual...
Page 185 Elevated temperatures might lead to deformation and leakage of the tubing system. Liquid spillage can damage electronic components of the instrument. • Do not use a water bath with more than 0.5 bar pressure. • Do not use liquids at temperatures above 50 °C. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 186: Analog To Digital Converter
Some detectors (for example CCD detectors) require an intermediate interface to convert analog data into a digital format that can be sent to the ChemStation (Analog In). The Agilent 7100 Capillary Electrophoresis System has a 20-bit AD converter built in. It enables voltage conversions ranging from 0 V to 1 V.
Page 187 Hardware Information Analog to Digital Converter Figure 56 CE part configuration Agilent 7100 Capillary Electrophoresis System User Manual...
Page 188 Hardware Information Analog to Digital Converter Agilent 7100 Capillary Electrophoresis System User Manual...
Page 189: Maintenance
Agilent 7100 Capillary Electrophoresis System User Manual Maintenance Standard Operating Procedures for Maintenance of Your Agilent CE Instrument Overview of Maintenance Early Maintenance Feedback (EMF) Cleaning the Electrodes, Pre-punchers and Insulation Plate Maintenance of the Replenishment System Changing the Air Inlet Filter...
Page 190: Standard Operating Procedures For Maintenance Of Your Agilent Ce Instrument
Maintenance tasks are supported by the Agilent Lab Advisor software or the Agilent Instrument Utilities software. It is delivered with the 7100 Agilent Lab Advisor software comes with a complete set of features. The Agilent Instrument Utilities software provides set of basic features.
Page 191: Overview Of Maintenance
Perform lamp intensity test from the Use the red-labeled alignment interface without detector test suite in the Lab Advisor capillary for tests. regularly (for example bi-weekly) and monitor lamp life using EMF values. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 192 N O T E function maintenance position from the tools section of the Lab Advisor. Careless opening may lead to floating of electrodes with buffer or sample which leads to contamination or carry-over. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 193 • Clean the replenishment tubing system • Change the air inlet filter • Change the buffer frit • Change the UV lamp • Clean the insulation plate • Perform a (full) system diagnostic test Agilent 7100 Capillary Electrophoresis System User Manual...
Page 194: Early Maintenance Feedback (Emf)
(high or low sensitivity analysis, wavelength, etc.), therefore, the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 195: Setting The Emf Limits
EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Please introduce self-made EMF counters to remind you to frequently clean the electrodes, N O T E pre-punchers and insulation plate. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 196: Cleaning The Electrodes, Pre-Punchers And Insulation Plate
• Reinstall the pre-punchers • Reinstall the insulation plate • Reinstall the electrodes Follow the common safety regulations for laboratories when doing this task. Wear safety N O T E glasses and rubber gloves. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 197: Prepare The Agilent Ce Instrument
Hot lamp WA R N I N G If you were using the Agilent CE instrument shortly before, the lamp may be very hot. • Avoid touching the lamp. • Wear protective gloves. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 198: Remove The Covers
Pull steadily on both sides. You will need slightly more force to remove the right side due to a connector. Turn the screws back to horizontal orientation. Screws Front cover Connector Figure 57 Removing the front cover Agilent 7100 Capillary Electrophoresis System User Manual...
Page 199: Accessing The Electrodes
3 Use the 12 mm hexagonal socket screwdriver to unscrew the inlet electrode. 12 mm hexagonal socket screwdriver High voltage cable, red Ground cable screw Ground cable, blue Figure 59 Removing the ground cable and the inlet electrode cable Agilent 7100 Capillary Electrophoresis System User Manual...
Page 200 It is not possible to remove the outlet electrode without taking out the insulation plate. N O T E 6 Remove the outlet electrode from the insulation plate using the 12 mm hexagonal socket screwdriver. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 201: Accessing The Liquid Handling Module
3 Carefully pull the liquid handling module out from the main frame. Pull steadily in the middle until you reach the end position. Carefully avoid damaging the replenishment needle. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 202 Maintenance Cleaning the Electrodes, Pre-punchers and Insulation Plate 1. Front screws 2. Take out bottles 3. Push liquid handling out Figure 61 Accessing the liquid handling module Agilent 7100 Capillary Electrophoresis System User Manual...
Page 203: Accessing And Removing The Pre-Punchers
N O T E Risk of injury due to sharp tips WA R N I N G The pre-punchers have very sharp tips. • Be careful when touching the tips of the pre-punchers. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 204 The teflon tube does NOT need to be removed from the pre-punchers for cleaning. If the N O T E connection between the tube and the pre-puncher is not tight, since the tube became wider, shorten the tube and shift it on the pre-puncher again. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 205: Cleaning The Electrodes
You may place it in a beaker of water or isopropanol and treat this in an ultrasonic bath for 5 minutes followed by steps 2 and 3 in this section. If this fails, replace the electrode. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 206: Cleaning The Pre-Punchers
Flushing pre-punchers 2 Flush the pre-punchers with water and isopropanol. 3 Use compressed air to dry the pre-punchers and remove all traces of liquid. Do not forget to dry the connected tubes as well. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 207: Cleaning The Insulation Plate
1 Moisten a tissue with water and use it to clean the insulation plate. 2 Moisten a tissue with isopropanol and use it to clean the insulation plate. 3 Dry the insulation plate with a tissue and compressed air. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 208: Reinstalling The Pre-Punchers
The fitting of the inlet pre-puncher must be tight. Otherwise, pressure or injection problems may result. 4 Carefully insert and tighten the plastic screw to secure the pre-puncher. The pre-puncher will assume the correct position as the screw is tightened. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 209 5 Carefully insert the replenishment pre-puncher. Tighten the screw to secure the pre-puncher. The pre-puncher will assume the correct position as the screw is tightened. The replenishment pre-puncher is not connected to a tube. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 210: Reinstalling The Electrodes And The Insulation Plate
(1.), then tilt it up into place (2.) and push it completely to the left (3.). 3. Left shift 2. Rotation 1. Insert Figure 67 Installation of the insulation plate Agilent 7100 Capillary Electrophoresis System User Manual...
Page 211 8 Insert the inlet electrode into the ring connector of the red high voltage wire. Thread the inlet electrode (and the ring connector) into the insulation plate by hand, then tighten the inlet electrode with the 12 mm hexagonal socket screwdriver. Do not overtighten. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 212 Take care not to damage tubes and flat ribbon cables on the right-hand side of the liquid N O T E handling module. Take care that the connectors on the left-hand side, for example for the drainage, fit into their cut-out in the back. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 213 However, under the following circumstances (usage of the alignment center), slow lifter movements with a low force are possible with the following alignment procedure. When you start any lifter movement your hands should be outside the instrument. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 214 N O T E still perform the check alignment procedure. The electrodes are fragile. Manual bending during alignment should be done only with N O T E great care. Electrodes must be kept straight. Avoid curving. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 215: Reinstall The Capillary Cassette
3 Start mounting the front cover by inserting it at the lower end first. 4 Carefully push the front cover into the main frame. You might need to pay special care on the right side due to a connector. 5 Close the lid of the instrument. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 216: Maintenance Of The Replenishment System
• the replenishment system will be idle for some time. Requirements • Vials and caps • Water and/or buffer and isopropanol (depending on the protocol selected) • ChemStation, Lab Advisor or Instrument Utilities software Agilent 7100 Capillary Electrophoresis System User Manual...
Page 217: Changing The Buffer Composition In The Replenishment System
This protocol suggests a combination of flush tubes first and a subsequent clean tubes step, with comparably low volume consumption, for a high exchange efficiency and low buffer consumption. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 218: The Replenishment System Will Not Be Used For Some Time
11 Execute the clean level sensing operation to clean the replenishment needle entirely. 12 Repeat all steps starting with number 2 and use isopropanol instead of water. Empty the waste bottle whenever you fill or refill the electrolyte bottle. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 219: Further Action
1 Proper seal of the electrolyte bottle 2 Proper seal of the waste bottle 3 Tight fittings If none of these reasons apply, call Agilent Technologies. Operations for Replenishment Maintenance The following operations can be started from the ChemStation, Lab Advisor or Instrument Utilities software.
Page 220 This function prompts you to load tray position 48 (or user input) with a vial filled with an appropriate cleaning liquid (usually water or current buffer system). When initiated, 8 cleaning cycles are performed. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 221 In such cases, we recommend exchanging the restrictor capillaries and or the replenishment needle by personnel trained by Agilent Technologies. If the replenishment system will not be used for some time the level sensor should be N O T E cleaned with water.
Page 222: Changing The Air Inlet Filter
If the instrument cannot build-up either pressure or a vacuum, check the following: • proper seal of the pressure bottle, • proper seal of the vacuum bottle, and • loose fittings on the bottles If none of these reasons apply, call Agilent Technologies. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 223 Changing the Air Inlet Filter Air inlet filter Figure 71 Air inlet filter Do not run the instrument without the air filter installed, since particles may damage the N O T E valve system. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 224: Changing The Lamp
It may happen that a hot lamp refuses to ignite the first time. Please wait until the lamp has cooled down. Only lamps provided by Agilent Technologies are supported in the 7100 CE instrument. The N O T E lamp tag stores various data and details on the ignition program of the deuterium lamp. A lamp without a tag will fail to ignite and could lead to instrument errors.
Page 225: Removing The Existing Lamp
1 Select Exit from the File menu to exit the Agilent ChemStation. Exit both online and offline copies. 2 Turn off the line power to the Agilent CE instrument. 3 Open the top cover. 4 Open the detector cover. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 226 Lamp screws Positioning pin Latch Connector Figure 72 Exchanging a lamp 6 Loosen the two lamp screws using the Pozidriv screwdriver and lift the lamp out carefully. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 227: Inserting A New Lamp
• correct seating and connection of the lamp cables, • correct seating of the cassette, and • if the green alignment interface without capillary is used, check for contamination of the interface. If none of these reasons apply, call Agilent Technologies. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 228: Cleaning The Instrument
Liquid dripping into the electronic compartment of your module. Liquid in the module electronics can cause shock hazard and damage the module. • Do not use an excessively damp cloth during cleaning. • Drain all solvent lines before opening any fittings. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 229: Parts And Materials For Maintenance
Agilent 7100 Capillary Electrophoresis System User Manual Parts and Materials for Maintenance Overview of Maintenance Parts This chapter provides information on the parts required for maintenance. Agilent Technologies...
Page 230: Overview Of Maintenance Parts
Screws for pre-puncher, 10/pk G1600-62402 Air filter, 5 µm 3150-0619 Capillary cassette G7100-60002 Optical filter for DAD, 260 nm G7100-62700 Vials Table 23 Alignment interfaces Table 24 Fuses “7100 Capillary Electrophoresis Instrument Electronics” on page 173 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 231: Appendix
Agilent 7100 Capillary Electrophoresis System User Manual Appendix General Safety Information Allgemeine Sicherheitsinformation Informations générales de sécurité Informazioni generali sulla sicurezza Información de seguridad ???????????? ?????? Setting Up a Test Sample Run Chemical and Biological Safety The Waste Electrical and Electronic Equipment (WEEE) Directive...
Page 232: General Safety Information
Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 233 When working with solvents please observe appropriate safety procedures (e.g. safety glasses, gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 234 C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. • Do not proceed beyond a caution until you have fully understood and met the indicated conditions Agilent 7100 Capillary Electrophoresis System User Manual...
Page 235: Allgemeine Sicherheitsinformation
Wartung und Reparatur des Gerätes zu beachten. Die Nichtbe- achtung dieser Vorsichtsmassnahmen bzw. der speziellen Warnungen inner- halb dieses Handbuchs verletzt die Sicherheitsstandards der Entwicklung, Herstellung und vorgesehenen Nutzung des Gerätes. Agilent Technologies übernimmt bei Nichteinhaltung dieser Vorschrift durch den Kunden keine Haf- tung.
Page 236 Beachten Sie bitte beim Arbeiten mit Lösungsmitteln die geltenden Sicher- heitsvorschriften (z. B. Tragen von Schutzbrille, Arbeitshandschuhen und Sicherheitskleidung), wie sie in den Sicherheitsdatenblättern des Herstellers beschrieben sind; dies gilt speziell für der Handhabung giftiger oder gesund- heitsgefährdender Lösungsmittel. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 237 Sie auf Situationen hin, die zu einem möglichen Datenverlust oder zu einer Beschädigung des Geräts führen können. • Fahren Sie bei einer Kennzeichnung durch diesen Sicherheitshinweis erst fort, wenn Sie diesen vollständig verstanden und entsprechende Maßnahmen getroffen haben. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 238: Informations Générales De Sécurité
à la conception, à la fabrication et à l’usage prévu de l’instrument. Agilent Technologies ne peut être tenu responsable du non-respect de ces exi- gences par le client.
Page 239 Lorsque vous manipulez des solvants, respectez les règles de sécurité (port de lunettes, de gants et de vêtements de protection) décrites dans la fiche de don- nées de sécurité fournie par le fournisseur du solvant, surtout si les solvants utilisés sont toxiques ou dangereux. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 240 • N'allez pas au-delà d'une mise en garde Attention tant que vous n'avez pas parfaitement compris et rempli les conditions définies. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 241: Informazioni Generali Sulla Sicurezza
Verificare che, in caso di sostituzione dei fusibili, vengano utilizzati solo quelli con la corrente nominale richiesta e del tipo specifico (normale, ad azione ritardata e così via). Evitare l'uso di fusibili riparati e il corto circuito delle sedi dei fusibili. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 242 Durante l'uso di solventi, osservare sempre le procedure di sicurezza idonee (ad esempio indossare bracciali ed abiti antinfortunistici) come descritto nella documentazione fornita con il materiale, specialmente in presenza di solventi tossici o pericolosi. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 243 L'indicazione AVVERTENZA AVVERTENZA indica situazioni che possono causare una perdita di dati o danni allo strumento. • Non procedere oltre finché non è stato compreso ed eseguito quanto indicato. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 244: Información De Seguridad
Agilent Technologies no se responsabiliza del incumplimiento de estos requisitos por parte del usuario.
Page 245 (guantes de seguridad, gafas y ropa adecuada) descritos en las especificaciones sobre el tratamiento de material y seguridad que suministra el proveedor de disolventes, especialmente cuando se utilicen productos tóxi- cos o peligrosos. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 246 • No continúe después de un mensaje de este tipo hasta que no lo haya comprendido perfectamente y se cumplan las condiciones indicadas. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 247: 安全に関する一般的な情報
理のすべての段階で遵守するようにしてください。以下の注意事項またはこのマニュアルの他の箇所に記載されている警告に従わないと、本機器の設計、製造、および意図された使用法に関する安全基準に違反することになります。ユーザーがこれらの要件を守らなかった場合、アジレントでは本製品の信頼性を保証することはできません。一般本製品は、国際安全規格に従って製造および試験された、安全クラスⅠの装置 ( 保護接地用端子付き ) です。操作電源を投入する前に、設置方法が本書の説明に合っているかどうか確認してください。さらに、次の注意を守ってください。操作中に装置のカバーを取り外さないでください。装置のスイッチを ON にする前に、すべての保護接地端子、延長コード、自動変圧器、および本装置に接続されている周辺機器を、接地コネクタを介して保護接地に接続してください。保護接地がどこかで途切れていると、感電によって人体に重大な危害を及ぼすことがあります。保護が正常に機能していないと思われる場合は、装置のスイッチを OFF にして、装置の操作を中止してください。ヒューズを交換する際は、必ず指定したタイプ ( 普通溶断、タイムラグなど ) と定格電流のヒューズだけを使用してください。修理したヒューズを使用したり、ヒューズホルダを短絡させたりしてはなりません。本書で説明した調整作業には、装置に電源を入れた状態で、保護カバーを取り外して行うものがあります。その際に、危険な箇所に触れると、感電事故を起こす可能性があります。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 248 Appendix 安全に関する一般的な情報機器に電圧をかけた状態で、カバーを開いて調整、メンテナンス、および修理を行うことは、できるだけ避けてください。どうしても必要な場合は、経験のある担当者が感電に十分に注意して実行するようにしてください。内部サービスまたは調整を行う際は、必ず応急手当てと蘇生術ができる人を同席させてください。メンテナンスを行うときは、必ず装置の電源を切って、電源プラグを抜いてください。本装置は、可燃性ガスや有毒ガスが存在する環境で操作してはなりません。このような環境で電気装置を操作すると、引火や爆発の危険があります。本装置に代替部品を取り付けたり、本装置を許可なく改造してはなりません。本装置を電源から切り離しても、装置内のコンデンサはまだ充電されている可能性があります。本装置内には、人体に重大な危害を及ぼす高電圧が存在します。本装置の取り扱い、テスト、および調整の際は十分に注意してください。特に、有毒または有害な溶媒を使用する場合は、試薬メーカーによる物質の取り扱いおよび安全データシートに記載された安全手順（保護眼鏡、安全手袋、および防護衣の着用など）に従ってください。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 249 Appendix 安全に関する一般的な情報安全シンボル表 32 安全シンボル記号説明危害のリスクを保護するために、そして装置を損傷から守るために、ユーザーが取扱説明書を参照する必要がある場合、装置にこの記号が付けられます。危険電圧を示します。保護接地端子を示します。本製品に使用されている重水素ランプの光を直視すると、目を傷める危険があることを示しています。表面が高温の場合に、この記号が装置に付けられます。加熱されている場合はユーザーはその場所を触れないでください。警告は、警告人身事故または死に至る状況を警告します。 • 指示された条件を十分に理解してそれらの条件を満たしてから、その先に進んでください。注意注意データ損失や機器の損傷を引き起こす状況を警告します。 • 指示された条件を十分に理解してそれらの条件を満たしてから、その先に進んでください。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 250: 一般安全信息
安全事项或本手册中其他位置的特殊警告事项，将违反此仪器设计、制造和使用的安全标准。安捷伦科技对用户不遵守这些要求所造成的损失不承担任何责任。一般说明本仪器为 I 级安全设备 ( 即提供保护接地端 )，并按国际安全标准制造与检测。操作通电前，应符合安装部分的要求。另外，还应遵循下列事项。操作时不得卸下仪器盖。启动仪器前，所有接地保护端、外接线、自耦变压器及所连接的设备都必须经接地插座进行保护接地。任何干扰保护接地的因素都将导致潜在的电击危险，可能引起严重的人身伤害。保护设施可能受到损害时，必须停止仪器操作，并将仪器保护起来以防有意地使用。确保只能用能够承受所要求的额定电流、并且为特定类型（正常烧断、时间延迟等）的保险丝进行更换。必须避免使用维修过的保险管 £¬ 而且要避免保险管套短路 ¡£ 本手册中所述的一些调节是在仪器通电时和仪器的保护盖卸下时进行的。许多位置带电，一旦接触就可能造成人身伤害。在仪器打开后，尽可能避免在通电时做调整、维护和维修。若必须进行上述活动，则应该由能意识到危险的技术人员进行。当现场不能提供紧急救护时，不要试图进行内部维护和调整。在电源线接通后，不要更换元件。在有易燃气体或蒸气存在时，不要操作仪器。在这种环境下操作任何电气仪器都肯定会有危险。不要在仪器上安装替换零件或对仪器进行未经授权的改造。即使仪器已经断电，仪器内部电容仍有可能带电。本仪器内部有能造成严重人身伤害的危险电压。在处理、测试和调整仪器时应特别小心。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 251 Appendix 一般安全信息当使用溶剂时，尤其是使用有毒或有害溶剂时，请遵循溶剂供应商在材料处理和安全数据表中所述的相应安全规程（例如戴上护目镜、安全手套，穿上防护衣）。安全标志表 33 安全标志标志说明对于标有此标志的设备，用户应参阅说明手册，以免对操作员造成伤害及仪器受到损坏。表示危险电压。表示受保护的接地端。表示用肉眼直接观察用于本产品的氘灯时，它所产生的光可能会损坏眼睛。如果存在较热表面，并且用户不应在加热后接触该表面，则仪器上会标有此标志。警告警告警告您可能导致伤亡的情况。 • 除非您已充分理解并满足了指定的条件，否则请勿超越警告范围进行工作。小心小心警告您可能导致数据丢失或设备损坏的情况。 • 除非您已充分理解并满足了指定的条件，否则请勿超越小心范围进行工作。 Agilent 7100 Capillary Electrophoresis System User Manual...
Page 252: Setting Up A Test Sample Run
Enter Vial location 7, enter sample name “1 mM pHAP” and click Run method to start the analysis. A typical electropherogram will show a significant peak at a migration time of around 3 minutes. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 253: Method Parameter Ce (Default Settings)
• Voltage 0.0 V (will ramp up in accordance with timetable) • Current 300 µA • Power 6.0 W • Low current limit OFF Stoptime • 10.0 minutes Posttime • OFF Replenishment • Replenishment and preconditioning parallel processing • No replenishment used Agilent 7100 Capillary Electrophoresis System User Manual...
Page 254 • Flush inlet 2, outlet 8 for 5 minutes • Flush inlet 6, outlet 8 for 10 minutes CE Injection • Inject by pressure, 50 mbar, 4 seconds Timetable • Change voltage 0.2 minutes, 30 kV Postconditioning • No postconditioning used Agilent 7100 Capillary Electrophoresis System User Manual...
Page 255: Method Parameter Dad
• > 0.1 minutes (2.0 s response time) (2.5 Hz) Stoptime • As with CE Posttime • Off Spectrum • None Autobalance • Prerun Autobalance Lamps Required for Acquisition • UV lamp Timetable • No entry Agilent 7100 Capillary Electrophoresis System User Manual...
Page 256: Chemical And Biological Safety
• Because leaks, spills, or loss of sample may generate aerosols, observe proper safety precautions. • Agilent 7100 CE instrument covers have not been designed as bioseals for aerosols or liquid containment. • Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of microorganisms.
Page 257: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)
“Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste. To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 258: Radio Interference
Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...
Page 259: Sound Emission
This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 7100 Capillary Electrophoresis System User Manual...
Page 260: Legal Notice
Appendix Legal Notice Legal Notice The Agilent 7100 Capillary Electrophoresis System is sold for research only. By purchasing this instrument, the purchaser is granted the limited right to use only this instrument. Purchase of this instrument does not include any...
Page 261: Agilent Technologies On Internet
For the latest information on products and services visit our worldwide web site at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the Agilent 7100 Capillary Electrophoresis System for download. Agilent 7100 Capillary Electrophoresis System User Manual...
Page 262 Appendix Agilent Technologies on Internet Agilent 7100 Capillary Electrophoresis System User Manual...
Page 263: Index
98, gradients capillary installation into cassette capillary cassette opening High Pressure removing home vials temperature humidity capillary conditioning Agilent 7100 Capillary Electrophoresis System User Manual...
Page 264 81, stoptime straight capillaries main system switching during run maintenance alignment interface method parameters 92, Telnet temperature environment noise level 107, test instrument timetable peak width tray Agilent 7100 Capillary Electrophoresis System User Manual...
Page 266 In This Book This manual contains technical reference information about the Agilent 7100 Capillary Electrophoresis System. The manual describes the following: • introduction and specifications, • installation, • using and optimizing, • troubleshooting, • maintenance • parts identification, • safety and related information.

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