Agilent Technologies Bravo Platform User Manual page 94

Pipetting or teachpoint problems
Problem
The Bravo head fails to put tips
on.
The tip box raises off the
platepad during Tips On
operations.
Tips Off operations are not
successful.
Tips are askew in the tip box or
not returned to their designated
well locations after Tips Off
operations.
The pipetting accuracy is
inconsistent or poor.
Liquid fails to pump into or drain
from an autofilling reservoir.
Bravo Platform User Guide
Potential cause
The tips are damaged or
deformed.
The labware definition for the tip
box is incorrect.
The deck location teachpoint is
incorrect for the installed liquid-
handling head. An Alignment
Station might be required for Tips
On operations.
The tip box stripper pins on the
liquid-handling head are retracted.
Stacked tips on a barrel. Static
electricity is causing the tip to cling
to the barrel during Tips Off
operations, and then a second tip
is pressed on during Tips On
operations.
Dropped tips. Static electricity is
causing tips to cling to the barrels
during Tips Off operations. As the
Bravo head moves, these tips drop
on top of a tip box or on the deck.
The tip box on the deck does not
match the labware definition
specified in the protocol.
The tip box location requires a
teachpoint adjustment.
The bias table in the liquid class
requires adjustment for your
pipetting device.
The liquid-handling head might
have damaged barrels or require
an O-ring replacement.
The Pump Module tubing has
deteriorated or is not connected
properly.
4 Maintenance and troubleshooting
Troubleshooting hardware problems
Solution
Repeat the operation using a fresh
box of good tips.
Verify that the correct tip box is in the
correct location and that the labware
definition is correct.
See "Setting teachpoints" on page
IMPORTANT
Pressing pipette tips
on the head requires a high degree of
precision in the positioning of the
head relative to the tip box, especially
for 384-well tip boxes. The Alignment
Station provides greater tip-loading
precision than a platepad.
Release the tip box stripper pins on
the liquid-handling head. See
"Retracting tip box stripper pins" on
page 75.
To resolve Tips Off problems caused
by static electricity, select the Enable
tips-off tip-touch option in the profile.
To resolve Tips Off problems caused
by static electricity, select the Enable
tips-off tip-touch option in the profile.
Verify that the correct tip box is in the
correct location.
See "Setting teachpoints" on page
See the Calibrating the pipettor topic
in the VWorks Automation Control
Setup Guide.
Contact Automation Solutions
Technical Support.
Inspect the tubing and the
connections, and replace, if
necessary.
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