Agilent Technologies Bravo Platform User Manual page 293

E Diagnostics quick reference
AM Mix task parameters
282
Parameter
Aspirate/dispense flow rate
(0–500 µL/s)
Mix cycles (0–100)
Dynamic tip extension
(0–20 mm/µL)
Description
The numerical value or the JavaScript variable
that will override both the aspirate and dispense
velocity in the selected liquid class.
A JavaScript variable enables the value to be
assigned later. For example, using a VWorks form,
an operator could easily change the flow rate for a
mix step in increments from as low as 1 µL/min
up to 2000 µL/min or more using the same liquid
class.
If the task is included in a VWorks macro, a
JavaScript variable enables you to change the
value for the task at the macro level.
IMPORTANT
The software requires that the
flow rate value be in microliters per second (µL/s)
at run time. If you want an operator to enter the
value in microliters per minute (µL/min), you can
use scripting to convert the values for the
software to use.
The number of times to repeat the aspirate-and-
dispense cycle.
The rate at which the Bravo 96AM Head moves
during the aspirate action. The software
calculates the distance over which the tips will
move without crashing.
Use dynamic tip extension to prevent spills as the
pipette tips displace the liquid.
To move the tips:
•
At the same rate as the volume change.
Calculate dynamic tip extension (DTE) as
follows:
DTE = (well depth)/(well vol) = 1/A, where A is
the cross-sectional area of a well with straight
walls
•
Faster than the volume change.
DTE > 1/A
•
Slower than the volume change.
DTE < 1/A
The starting and ending positions can be
calculated as follows:
(V * DTE) + Distance
aspirated
Note: Instead of a negative aspirated volume, the
software automatically moves downward toward
the well bottom with each aspirate action.
Bravo Platform User Guide
well bottom