Agilent Technologies Bravo Platform User Manual page 277

E Diagnostics quick reference
Wash Tips task parameters
266
Parameter
Dynamic tip extension
(mm/(µL)
Perform tip touch
Which side to perform tip touch
Tip touch retract distance
Tip touch horizontal offset
Description
The rate at which the pipette head moves during
the Wash Tips task. The software calculates the
distance over which the tips will move without
crashing.
Use dynamic tip extension to prevent spills as the
pipette tips displace the liquid.
To move the tips:
•
At the same rate as the volume change.
Calculate dynamic tip extension (DTE) as
follows:
DTE = (well depth)/(well vol) = 1/A, where A is
the cross-sectional area of a well with straight
walls
•
Faster than the volume change.
DTE > 1/A
•
Slower than the volume change.
DTE < 1/A
The starting and ending positions can be
calculated as follows:
(V * DTE) + D
dispensed well bottom
(V * DTE) + D
aspirated well bottom
The option to touch the pipette tip on one or more
sides of the well.
The wall or walls for tip touch: North, South, East,
West, North/South, West/East, West/East/South/
North.
If you also select the Dispense to waste during
wash option, the tip touch is performed on the
northeast side only.
The vertical distance the pipette tips rise before
touching the sides of the wells.
The horizontal distance the tips move. The value
is based on the well diameter specified by the
labware definition.
The value of the parameter determines the
direction of movement:
•
0. Tips move a horizontal distance equal to
the well radius.
•
Great than 0. Tips attempt to move past the
well radius, which results in a more forceful
tip touch.
•
Less than 0. Tips move a distance less than
the radius of the well, resulting in a lighter tip
touch.
Bravo Platform User Guide