Agilent Technologies Bravo Platform User Manual page 285

E Diagnostics quick reference
AM Aspirate task parameters
274
Parameter
Dynamic tip extension
(0–20 mm/µL)
Pipette technique
Perform tip touch
Which sides to use for tip touch
Tip touch retract distance
(–20 to 50 mm)
Description
The rate at which the Bravo 96AM Head moves
during the AM Aspirate task. The software
calculates the distance over which the tips will
move without crashing.
Use dynamic tip extension to prevent spills as the
pipette tips displace the liquid.
To move the tips:
•
At the same rate as the volume change.
Calculate dynamic tip extension (DTE) as
follows:
DTE = (well depth)/(well vol) = 1/A, where A is
the cross-sectional area of a well with straight
walls
•
Faster than the volume change.
DTE > 1/A
•
Slower than the volume change.
DTE < 1/A
The starting and ending positions can be
calculated as follows:
(V * DTE) + Distance
aspirated
Note: Instead of a negative aspirated volume, the
software automatically moves downward toward
the well bottom with each aspirate action.
The pipette location offset you want to use for the
AM Aspirate task.
The list of pipette techniques are defined in the
Pipette Technique Editor.
The option to touch the pipette tip on one or more
sides of the well.
The side or sides of the well to use during tip
touch: North, South, East, West, North/South,
West/East, West/East/South/North.
The vertical distance for the pipette tips to rise
before touching the sides of the wells.
Bravo Platform User Guide
well bottom