Agilent Technologies Bravo Platform User Manual page 262

Bravo Platform User Guide
Parameter
Dynamic tip retraction
(0–20 mm/µL)
Pipette technique
Perform tip touch
Which sides to use for tip touch
Tip touch retract distance
(–20 to 50 mm)
Tip touch horizontal offset
(–5 to 5 mm)
E Diagnostics quick reference
Dispense task parameters
Description
The rate at which to raise the pipette head during
the Dispense task.
Use dynamic tip retraction to prevent spills as the
pipette tips displace the liquid.
To move the tips:
•
At the same rate as the volume change.
Calculate dynamic tip retraction (DTR) as
follows:
DTR = (well depth)/(well vol) = 1/A, where A is
the cross-sectional area of a well with straight
walls
•
Faster than the volume change.
DTR > 1/A
•
Slower than the volume change.
DTR < 1/A
The starting and ending positions can be
calculated as follows:
(V * DTR) + Distance
dispensed
The pipette location offset you want to use for the
Dispense task.
The list of pipette techniques are defined in the
Pipette Technique Editor.
The option to touch the pipette tip on one or more
sides of the well.
The side or sides of the well to use during tip
touch: North, South, East, West, North/South,
West/East, West/East/South/North.
The vertical distance for the pipette tips to rise
before touching the sides of the wells.
The horizontal distance the tips move. The value
is based on the well diameter specified by the
labware definition.
For example, if you set a value of:
•
0, the tips move a horizontal distance equal to
the well radius
•
> 0, the tips attempt to move past the well
radius, which results in a more forceful tip
touch
•
< 0, the tips move a distance less than the
radius of the well, resulting in a lighter tip
touch
well bottom
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