Agilent Technologies Bravo Platform User Manual page 213

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D AssayMAP Bravo Platform
Bravo 96AM Head overview
Liquid-handling modes
202
The Bravo 96AM Head features include the ability to:
Transfer liquids directly with the bare probes, through AssayMAP Bravo cartridges
mounted on the probes, and using mounted 250-µL large-transfer (LT) pipette tips
Mount or remove the cartridges while holding liquid in the syringes
Provide precise control of flow rates and volume required for moving fluid through
the packed resin in the cartridges
Access 96-well and 384-well microplates
Perform 96-channel liquid handling or partial-head liquid handling using a subset of
the 96 channels
Protocols for the Bravo 96AM Head can employ a combination of the following liquid-
handling modes:
Bare probes
AssayMAP Bravo cartridges mounted on the probes
250-µL LT pipette tips mounted on the probes
Capabilities of each liquid-handling mode
The following figure shows a side-by-side comparison of a bare probe, a probe with a
mounted cartridge, and a probe with a mounted 250-µL LT tip. The following table
provides the details for each.
Figure
Comparison of probe, probe with cartridge, and probe with 250-µL LT tip
1
2
3
Item
Mode
Description
1
Bare probe
Maximum volume: 250 µL can be aspirated into the
syringe before the syringe is full.
Only 96-channel (full head) liquid handling is supported.
As the tips of the syringes, the probes can be in direct
contact with the samples for liquid-handling tasks.
Bravo Platform User Guide

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