General Precautions For Connecting Capillaries - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
TCC-3000SD and TCC-3000RS Column Compartments

4.2 General Precautions for Connecting Capillaries

When connecting capillaries, observe the following general precautions:
• Observe the precautionary statements for capillaries and capillary connections in
section 1.2.2 (→ page 4).
• When connecting the capillaries, make sure that the connectors are free from
contaminants. Even minute particles may cause damage to the system, for example, to
the column.
• Different fitting systems are used in an UltiMate 3000 system. Install the capillaries and
fittings only at the positions for which they are intended.
• Thermo Fisher Scientific recommends using Viper capillary connections whenever
possible. When using Viper capillaries, observe the information in the instructions
shipped with the capillary.
• Depending on the fitting connection, also observe the following:
♦ Viper fitting connections
Loosen and tighten Viper fitting connections only by the knurled screw and only
with your hand (do not use tools).
First, tighten the screw hand-tight. If the connection leaks, tighten the screw a little
more. If leakage continues, remove the capillary, clean the capillary ends carefully
by using a cloth or tissue wetted with isopropanol, and reinstall the capillary. If the
connection continues to leak, replace the Viper capillary.
Tips: You can reuse capillaries with Viper fitting connections also for a
♦ Conventional fitting connections (non-Viper)
Do not over-tighten these fitting connections. If you observe leakage on the
connection, tighten a little further.
If leakage still exists, clean the connection port with a cleaning swab (part no.
6040.0006). Replace the capillary and/or fitting if this does not eliminate the
problem.
Reuse used fittings and ferrules only for the same capillary connection. This is to
avoid increased dead volume or damage to the system and leakage.
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different connection.
If the knurled screw is a black screw (as shown in Fig. 13), you can
remove it from the capillary and reattach it later again.
Fig. 13: Viper fitting connection (example)
Operating Instructions