Equilibrating The System - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

4.10 Equilibrating the System

Before using the TCC for sample analysis, equilibrate the UltiMate 3000 system:
1. Pump the starting solvent through the entire system until the system is free of any
other liquid composition.
2. Heat or cool all temperature-controlled devices to the temperature required for the
application.
3. Set the detector wavelengths and turn on the lamps.
4. Monitor the pump pressure. Verify that the reading is correct for the application and is
stable.
5. Monitor the detector signal and verify that the baseline signal is at the expected
reading for your application and is stable.
Perform system equilibration in Chromeleon or select the required commands and
parameters on the front panel menus of the instruments.
To equilibrate the system from Chromeleon
• Select and perform the operating commands and parameters from the Commands dialog
box (→ page 62).
Create and run an equilibration program to automate the process (→ page 65).
•
• Use the SmartStartup Wizard to create and run the equilibration program (see the next
section).
To create the equilibration program with the SmartStartup Wizard
1. To open the wizard, select SmartStartup on the Batch menu.
2. Follow the instructions as they appear on each page of the wizard. For additional
information about a page, click Help.
3. After you finish the wizard, Chromeleon
♦ Generates an equilibration program and sequence.
♦ Opens the equilibration control panel for the instruments in the timebase (→ Fig. 30,
page 58).
♦ Opens the Start Batch on dialog box.
Click Start to begin equilibration.
Operating Instructions
TCC-3000SD and TCC-3000RS Column Compartments
UltiMate 3000 Series:
Page 57