Post-Column Cooler - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
TCC-3000SD and TCC-3000RS Column Compartments
The decision which pre-column heater is appropriate for the respective application depends
on various factors, such as the flow rate and the temperature difference between the
ambient temperature and the column temperature. For applications in analytical HPLC and
UHPLC, the 2 µL pre-column heater usually provides the best performance. The 1 µL pre-
column heater is used, for example, with applications for which an especially low extra-
column volume is important. When using system capillaries with an inner diameter of >
180 µm and large-volume separation columns (semipreparative HPLC), Thermo Fisher
Scientific recommends installing a pre-column heater with a volume of 7 µL or 11 µL.

2.11 Post-Column Cooler

Only TCC-3000RS
The TCC-3000RS supports post-column cooling of the eluent. A post-column cooler insert
(SST) with a thermally effective volume of 2 µL (total volume: 5 µL) is shipped in the
accessories kit of the TCC-3000RS.
The post-column cooler can reduce the temperature of the column eluate to a temperature that
is appropriate for the subsequent detection (ideally the flow cell temperature), and thus
minimize the noise values. In this way, optimal detection results will be achieved.
With a column temperature of 100 °C and water used as eluent at a flow rate of 3 mL/min, the
column eluate can be cooled by at least 40 °C. The lower temperature limit for post-column
cooling is 5 °C. The upper temperature limit is 110 °C. The maximum heat dissipation of the
cooler is limited.
The post-column cooler can be used in the pH range 1-13. For information about how to
install the post-column cooler insert, see page 52. For information about how to operate the
TCC with post-column thermostatting, see page 74.
Operating Instructions Page 25