General Precautions For Connecting Capillaries - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

UltiMate 3000 Series:
Fluorescence Detectors FLD-3100 and FLD-3400RS

4.2 General Precautions for Connecting Capillaries

When connecting capillaries, observe the following general precautions:
• Observe the precautionary statements for capillaries and capillary connections in
section 1.2.2 (→ page 5).
• UltiMate 3000 systems with stainless steel fluidics are shipped with Viper capillary kits.
The kits include a Viper capillary for connecting the detector to the separation column
when the system includes only one detector. For UltiMate 3000 RSLC systems, you can
use the post column cooler to connect the detector to the column as an alternative. This is
recommended if you are operating the column at temperatures above 60°C, as high
temperatures may reduce the intensity of the fluorescence signal.
• If you are using more than one detector in a system, for example, a UV detector and a
fluorescence detector, another Viper capillary is available in the flow cell accessories.
• If possible, use a Viper capillary between the column and the detector. In rare cases, this
may not be possible for columns from third-party manufacturers. Always make sure that
the ID of replacement capillary corresponds to the ID of the capillary shipped with the
system.
• Depending on the fitting connection, also observe the following:
♦ Viper fitting connections
Loosen or tighten the Viper connection only using the black knurled screw and only
with your hand (do not use tools). The knurled screw can be easily removed and
reattached to the capillary at any time. Viper capillaries are designed to be leak-free
for all pressures that arise in UltiMate 3000 systems simply by tightening them with
your hand. If you observe leakage on the connection, tighten the screw a little further.
If leakage continues, remove the capillary, clean the capillary ends carefully by using
a cloth or tissue wetted with isopropanol, and reinstall the capillary. If the connection
continues to leak, replace the Viper capillary.
When connecting the Viper capillary to the flow cell inlet, observe the guidelines in
the Installation Instructions shipped with the capillary.
Capillaries with Viper fitting connections can be reused also for a different
connection.
Page 46 Operating Instructions