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Contacting Us Contacting Us You can find information on all of the different support services of Thermo Fisher Scientific by navigating the following website: https://www.thermofisher.com/us/en/home/technical-resources/ contact-us.html Charged Aerosol Detectors (VH-D20, VF-D20) Page 3 Operating Manual...
2.4.3 Allowed Concentrations ................ 28 2.4.4 Further Information ................. 28 Compliance Information .................. 29 3 Device Overview................. 31 Detector Features .................... 32 Operating Principle .................... 33 Interior Components.................... 35 Nebulizer...................... 36 Charged Aerosol Detectors (VH-D20, VF-D20) Page 5 Operating Manual...
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Connecting the Inlet Capillary to the Nebulizer ........ 77 5.6.6 Guiding Liquid Leaks to Waste .............. 81 Turning On the Device .................. 82 Setting Up the Device in the Software .............. 83 Page 6 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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6.10.3 Data Collection Rate and Filter Constant .......... 108 6.10.4 Signal Channels .................. 109 6.10.5 Evaporation Temperature .............. 110 6.10.6 Gas Regulation Mode (VH-D20 Detector only) ........ 112 6.10.7 Power Function Value ................ 112 6.10.8 Baseline Noise .................. 113 6.11 Shutting Down the Device ................. 114 6.11.1 Short-Term Shutdown (Interruption of Operation) .......
Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Charged Aerosol Detectors (VH-D20, VF-D20) Page 11 Operating Manual...
The descriptions in this manual assume that the device is installed in the Vanquish system stack. If this is not the case, additional hardware is required and must be ordered separately. The information in this manual applies correspondingly. Page 12 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Highlights information necessary to prevent damage to the device or invalid test results. TIP Highlights information of general interest or helpful information that can make a task easier or optimize the performance of the device. Charged Aerosol Detectors (VH-D20, VF-D20) Page 13 Operating Manual...
The electronic version (PDF) of the manual contains numerous links that you can click to go to other locations within the manual. These include: • Table of contents entries • Index entries • Cross-references (in blue text) Page 14 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Quick Start Guide. • Reference Card For a concise overview of the most important workflows, refer to the Reference Card. TIP The Chromeleon Help and documents are included in the software shipment. Charged Aerosol Detectors (VH-D20, VF-D20) Page 15 Operating Manual...
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Safety Data Sheets (SDSs). Third-party documentation includes the user documentation for the gas supply, as applicable: • User documentation for the nitrogen generator • User documentation for the air compressor Page 16 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
• Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device. Charged Aerosol Detectors (VH-D20, VF-D20) Page 17 Operating Manual...
Always keep the manual near the device for quick reference. • Save this manual and pass it on to any subsequent user. Read, understand, and comply with all safety messages and precautionary statements presented in this manual. Page 18 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
TIP An additional type label on the leak tray of the device indicates the module name, serial number, part number, and revision number (if any). To facilitate device identification, have the information from this label available when communicating with Thermo Fisher Scientific. Charged Aerosol Detectors (VH-D20, VF-D20) Page 19 Operating Manual...
Regularly running calibration • Establishing shelf life limits and following them for all consumables used with the system • Running the system according to the laboratory's verified and validated 'lab developed test' protocol Page 20 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Thermo Fisher Scientific before proceeding. Safety Standard This device is a Safety Class I instrument (provided with terminal for protective grounding). The device has been manufactured and tested according to international safety standards. Charged Aerosol Detectors (VH-D20, VF-D20) Page 21 Operating Manual...
Protective Clothing To protect you from chemical splashes, harmful liquids, or other contamination, put on appropriate protective clothing, such as a lab coat. Page 22 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Instead, place liquid reservoirs in the solvent rack that is available for the Vanquish system. 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the device: Charged Aerosol Detectors (VH-D20, VF-D20) Page 23 Operating Manual...
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Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. Page 24 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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(for example, pure acetonitrile). Discharge of electrostatic energy might lead to sparking, which could constitute a fire hazard. Prevent the generation of static electricity near the chromatography system. Charged Aerosol Detectors (VH-D20, VF-D20) Page 25 Operating Manual...
• Safety 2.3.6 In Case of Emergency WARNING—Safety Hazard In case of emergency, disconnect the device from the power line. Page 26 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
9.5 together with optical detectors. This can impair the functionality and optical performance of the detector flow cell. Observe the mobile phase guidelines for the detector. See Mobile Phase Guidelines for the Detector (} page 96). Charged Aerosol Detectors (VH-D20, VF-D20) Page 27 Operating Manual...
Vanquish system, refer to the Specifications chapter in the Operating Manual for the modules. • Refer also to the Operating Manuals for all modules in the Vanquish system. They may provide additional guidelines and information. Page 28 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Thermo Fisher Scientific or one of its authorized representatives. The device has been shipped from the manufacturing site in a safe condition. See also Compliance Information (} page 180) Charged Aerosol Detectors (VH-D20, VF-D20) Page 29 Operating Manual...
• Device Overview 3 Device Overview This chapter introduces you to the device and the main components. Charged Aerosol Detectors (VH-D20, VF-D20) Page 31 Operating Manual...
FocusJet™ nebulizer with concentric design for extended low pump flow rate range • Adjustable evaporation temperature for optimization of analyte response and use with a wider range of eluents VH-D20 detector: Adjustable range for the evaporation ¨ temperature VF-D20 detector: Selectable evaporation temperatures ¨...
Mobile phase from column Nebulizer Gas inlet Gas filters Spray chamber Drain pump Evaporation tube Mixing chamber Charger gas inlet Corona charger Ion trap Electrometer (detection chamber) Electrometer board Gas exhaust Charged Aerosol Detectors (VH-D20, VF-D20) Page 33 Operating Manual...
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(no. 12). Signal is processed by the electrometer board (no. 13) and sent to the chromatography data system. The resulting gas flow exits the detector through the gas exhaust (no. 14). Page 34 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Gas filter assembly behind the protective cover Leak tray Nebulizer (after installation) Waste outlet Type label, indicating the module name, serial number, part number, and revision number (if any) Drain port Leak sensor Charged Aerosol Detectors (VH-D20, VF-D20) Page 35 Operating Manual...
Eluate from the column flows into the nebulizer and merges with filtered gas that is supplied to the nebulizer. Due to the concentric flow design of the nebulizer, an aerosol is produced within the spray chamber. Figure 3: FocusJet nebulizer Page 36 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
(bottom filter) and then in the HEPA gas filter (top filter). Sufficient filtering of the supplied gas, both externally and internally, ensures that the gas is clean and free from particles. Figure 4: Gas filter assembly behind protective cover Charged Aerosol Detectors (VH-D20, VF-D20) Page 37 Operating Manual...
When the leak sensor detects leakage, the status indicators change to red and beeping starts to alert you. Follow the instructions in this manual to find and eliminate the source for the leakage. Page 38 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
For details, contact the Thermo Fisher Scientific sales organization. A keypad is available inside the device, allowing you to perform certain basic functions directly from the device. Charged Aerosol Detectors (VH-D20, VF-D20) Page 39 Operating Manual...
• Unpacking 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery. Charged Aerosol Detectors (VH-D20, VF-D20) Page 41 Operating Manual...
This will damage the doors or the device. Tools required Screwdriver, Torx Follow these steps 1. Place the shipping container on the floor and open it. 2. Remove the ship kit from the shipping container. Page 42 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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7. Some surfaces including the doors of the device are covered by a protective film during shipment. Remove the protective film from all surfaces as applicable. Charged Aerosol Detectors (VH-D20, VF-D20) Page 43 Operating Manual...
Ship Kit • Operating manual (downloadable from customer manual web site) • Power cord For information on contents of the ship kit or reordering parts, see Accessories, Consumables and Replacement Parts (} page 173). Page 44 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the device in the Vanquish system and in the chromatography software. Charged Aerosol Detectors (VH-D20, VF-D20) Page 45 Operating Manual...
Always unplug the power cord before starting repair work inside the device. • If you were instructed to remove any housing covers or panels, do not connect the power cord to the device while the cover or panels are removed. Page 46 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
5. Set up the device in the software. See Setting Up the Device in the Software (} page 83). 6. Prepare the system for operation. Refer to the Vanquish System Operating Manual. Charged Aerosol Detectors (VH-D20, VF-D20) Page 47 Operating Manual...
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If you have to move the device after it has been set up and installed in the Vanquish system, prepare the device for transport and move it to the new location. Follow the instructions in Transporting or Shipping the Device (} page 145). Page 48 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
The end of the power cords that plugs into the power socket on the device is identical for all power cords. The end of the power cords that plugs into the wall socket is different. Charged Aerosol Detectors (VH-D20, VF-D20) Page 49 Operating Manual...
If you suspect that condensation is present, allow the device to warm up to room temperature. This may take several hours. Wait until the condensation is gone completely before connecting the device to the power line. Page 50 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Direct the gas exhaust tubing to a fume hood or connect it to another ventilation device such as an industrial vent. Prevent a gas- tight connection and siphons between the gas exhaust port of the detector and the ventilation. Charged Aerosol Detectors (VH-D20, VF-D20) Page 51 Operating Manual...
Before you start the replacement of the gas filter assembly, make sure that the gas supply and the detector gas flow are turned off. • Observe the gas supply requirements. See Gas Supply Requirements (} page 52). Page 52 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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¨ TIP Use of smaller compressed nitrogen cylinders or liquid nitrogen containers may be a quick and easy source of nitrogen during installation. However, it is not recommended for routine operation. Charged Aerosol Detectors (VH-D20, VF-D20) Page 53 Operating Manual...
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• Make sure that the gas supply is ready for operation. Follow the instructions in the User Documentation for the gas supply. Page 54 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
To access the interior components in the device, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the device are located directly behind the doors. Figure 6: Opening the front doors Charged Aerosol Detectors (VH-D20, VF-D20) Page 55 Operating Manual...
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Note: The charged aerosol detector needs to be at the end of the flow path because it is a destructive detector. 5. Install the column compartment. Refer to the Operating Manual for the column compartment. Charged Aerosol Detectors (VH-D20, VF-D20) Page 57 Operating Manual...
Certain problems in the charged aerosol detector cause an automatic stop of the pump flow to prevent a build-up of excess liquid inside the detector. Page 58 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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See System Arrangement (} page 56). 2. Connect the required interface cables to the detector. See Connecting the Interface Cables (} page 60). 3. Connect the power cord (see Connecting the Power Cord (} page 62)). Charged Aerosol Detectors (VH-D20, VF-D20) Page 59 Operating Manual...
This picture shows the USB and system interlink connections if the charged aerosol detector is the second detector in the system, for example, after a diode array detector. Page 60 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Figure 9: Interface cable connections in the Vanquish system with charged aerosol detector and diode array detector Description System base Pump Autosampler Diode array detector Charged aerosol detector Column compartment Connection to computer Charged Aerosol Detectors (VH-D20, VF-D20) Page 61 Operating Manual...
• To avoid personal injury, always wear safety glasses when handling the nebulizer. • Do not touch the nebulizer tip. • Handle the nebulizer with care. Page 62 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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• Powder-free gloves • FocusJet nebulizer with dedicated nebulizer gas pressure certificate • Optional: Deionized water Preparations 1. Wear powder-free gloves. 2. Remove the packed nebulizer from behind the detector doors. Charged Aerosol Detectors (VH-D20, VF-D20) Page 63 Operating Manual...
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Do not to touch the nebulizer tip with the protective tubing, as it can damage the tip. Keep the protective tubing to have it available when transporting the nebulizer. Figure 11: Nebulizer with protective tubing and plug on the flow inlet Page 64 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Store it under clean conditions, for example, in the bag the nebulizer was delivered in to prevent contaminations at the nebulizer opening. Figure 12: Dust seal plug installed in the nebulizer opening Charged Aerosol Detectors (VH-D20, VF-D20) Page 65 Operating Manual...
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Figure 13: Correct orientation of the nebulizer for installation Description Center of the nebulizer opening Gripping sides on top and bottom handle side Protruding sides on left and right handle side Page 66 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Check that the protruding sides on the nebulizer arrest behind the two locks of the opening and lie against the alignment pin. Figure 15: Locking the nebulizer in the detector Description Locks on the nebulizer opening Alignment pin Charged Aerosol Detectors (VH-D20, VF-D20) Page 67 Operating Manual...
¨ particles may cause damage to the system or lead to invalid test results. Do not install gas tubes or connection unions that are stressed, ¨ nicked, kinked, or otherwise damaged. Page 68 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Figure 16: Connecting gas tubes to the elbow connectors Description Connection stem to gas inlet port Connector for gas inlet tubing Gas inlet tubing Connection stem to gas exhaust port Connector for gas exhaust tubing Gas exhaust tubing Charged Aerosol Detectors (VH-D20, VF-D20) Page 69 Operating Manual...
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Figure 17: Pushing in the connection unions Description Gas inlet port Gas inlet elbow connector with gas inlet tubing Gas exhaust port Gas exhaust elbow connector with gas exhaust tubing Page 70 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
To flush the Vanquish modules, follow the instructions in the Vanquish System Operating Manual. • Flushing the charged aerosol detector will be performed later during equilibration. Charged Aerosol Detectors (VH-D20, VF-D20) Page 71 Operating Manual...
Thermo Fisher Scientific. • The connectors must be free from contaminants. Even minute particles may cause damage to the system or lead to invalid test results. Charged Aerosol Detectors (VH-D20, VF-D20) Page 73 Operating Manual...
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TIP Note the slot in the knurl. You can easily remove the knurl from the capillary through this slot if space is limited. 3. Check whether the connection leaks. If leakage exists, follow the steps further down. Page 74 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
2. On the waste outlet on the detector, remove the plug. Follow these steps 1. Route the waste line from the system base to the detector, through the tubing guides of the system modules below the detector. Charged Aerosol Detectors (VH-D20, VF-D20) Page 75 Operating Manual...
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TIP The waste line should go straight to the system base and to waste. Make sure that the line is positioned straight in the tubing guides. Page 76 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Nebulizer (} page 78) Charged aerosol detector Connect the capillary Connecting the Capillary is the second detector in between flow cell and Between Flow Cell and the system nebulizer Nebulizer (} page 78) Charged Aerosol Detectors (VH-D20, VF-D20) Page 77 Operating Manual...
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The charged aerosol detector must be the last detector connected in the flow path. • Other detectors contribute to extra-column volume, which may have an adverse effect on the peak shape measured by the charged aerosol detector. Page 78 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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4. Position the capillary behind the capillary clip. 5. Carefully release the clip and take care not to clamp the capillary. The capillary must be secured behind the clip. Charged Aerosol Detectors (VH-D20, VF-D20) Page 79 Operating Manual...
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Figure 24: Series flow connection between diode array detector and charged aerosol detector (example) Description Capillary connection from the column compartment to the flow cell Capillary connection between flow cell and nebulizer Waste line from the charged aerosol detector to the system base Page 80 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
For information about how the liquid is discharged to waste through the Vanquish drain system, refer to the Vanquish System Operating Manual. Charged Aerosol Detectors (VH-D20, VF-D20) Page 81 Operating Manual...
Preparing the Device for Operation (} page 93). See also Power On/Off Control (} page 92) Page 82 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual. The Help for the software that you are using provides detailed information about the settings on each property page. Charged Aerosol Detectors (VH-D20, VF-D20) Page 83 Operating Manual...
• Operation 6 Operation This chapter describes the elements for device control, provides information for routine operation and for shutdown. Charged Aerosol Detectors (VH-D20, VF-D20) Page 85 Operating Manual...
Operating Manual. Details on control and operation of the device are available in the Chromeleon Help. Software descriptions in this manual refer to Chromeleon 7. Terminology may be different to that of other software versions. Page 86 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
When using tetrahydrofuran (THF), the use of air as supplied gas may pose an explosion hazard. This can pose a safety and health risk and damage the detector. Always use nitrogen with tetrahydrofuran or other highly combustible solvents. Charged Aerosol Detectors (VH-D20, VF-D20) Page 87 Operating Manual...
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Contamination of the mobile phase can damage the detector. Avoid contamination of the mobile phase. See Mobile Phase Guidelines for the Detector (} page 96). • Observe the specifications for operation. See Performance Specifications (} page 170). Page 88 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
The STATUS LED provides a quick visual check of the operational status of the device. When the doors are closed, the LED bar on the front side indicates the operational status. For status details, see Status Indicators (} page 90). Charged Aerosol Detectors (VH-D20, VF-D20) Page 89 Operating Manual...
The power to the device is turned off. Dimmed The doors of the device are open. Yellow, flashing slowly The power to the device is turned on, but the device is not connected in the chromatography data system. Page 90 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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A problem or error has occurred. For the related message, check the Instrument Audit Trail. For remedial action, see Troubleshooting (} page 155). For information about the LED that is present next to the GAS button on the keypad, see Keypad (} page 89). Charged Aerosol Detectors (VH-D20, VF-D20) Page 91 Operating Manual...
Check the Instrument Audit Trail for the related message and take appropriate remedial action. After the self-test, a warm-up phase follows. During this phase, the detector monitors the temperature of the internal components. Page 92 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Turn on the pump flow to the detector. Have the pump deliver flow to the detector. • Verify that any air bubbles are completely flushed out of the system flow path. Charged Aerosol Detectors (VH-D20, VF-D20) Page 93 Operating Manual...
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Turning on the lamp (or lamps) in the UV/VIS detector • Monitoring the pump pressure and pressure ripple and checking that the pressure is stable and the ripple within reasonable limits for the application Page 94 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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TIP The Chromeleon software supports procedures for automatically starting a chromatography system in the software (Smart Startup). The startup procedure includes the operations for system equilibration. For details, refer to the Chromeleon Help. Charged Aerosol Detectors (VH-D20, VF-D20) Page 95 Operating Manual...
In many cases, it is preferable to use materials dedicated for use only with volatile mobile phases. • NP solvents: NP solvents are highly soluble in the gas and might condensate in the gas exhaust tubing. Page 96 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
TIP With solvent gradients, non-volatile impurities in the more polar solvent (for example, water in reversed-phase) can be concentrated on the column and elute later to produce large peaks or baseline artifacts. Charged Aerosol Detectors (VH-D20, VF-D20) Page 97 Operating Manual...
The mobile phase reservoirs should be clean borosilicate glass that is dedicated for use with volatile mobile phases. In some applications, however, it may be necessary to use plastic reservoirs, for example when using extreme pH aqueous eluents (such as > pH 12). Page 98 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Overview of typical additives and buffers for use with the detector Additive/Buffer Buffer Recommended pH at max. range max. concentration concentration in water Acetic acid 4.76 3.8 – 5.8 0.3% v/v Ammonium 4.76 3.8 – 5.8 10 mM acetate Ammonium 7.8, 9.8, 7 – 11 10 mM bicarbonate 10.3 Charged Aerosol Detectors (VH-D20, VF-D20) Page 99 Operating Manual...
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Always make pH measurements in separate aliquots of the aqueous solution to avoid cross- contaminating the mobile phase buffers with non-volatile salts from the pH electrode calibrations. Page 100 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
If the nebulizer serial number deviates from the serial number on the nebulizer certificate, enter the serial number from the nebulizer certificate. 4. Close the Command window. 5. Monitor the Gas Regulator Pressure until it reaches the setpoint. Charged Aerosol Detectors (VH-D20, VF-D20) Page 101 Operating Manual...
The protective cover of the gas filter assembly is installed. ¨ 3. Turn on the gas supply to the detector. Observe the Gas specifications for the gas supply pressure. See Performance Specifications (} page 170). Page 102 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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2. Wait at least 5 minutes and only then the detector or using the turn off the detector gas flow using the chromatography data system. GAS button on the keypad of the detector or using the chromatography data system. Charged Aerosol Detectors (VH-D20, VF-D20) Page 103 Operating Manual...
The default unit setting for the gas pressure corresponds to the Pressure Unit that is defined on the detector configuration pages in the Chromeleon software. For further information, see Signal Channels (} page 109). Page 104 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Two modes for regulating the nebulizer gas pressure in the detector are available: Analytical mode and MicroLC mode. For details on the gas regulation modes, see Regulation Mode (VH-D20 Detector only) (} page 112). Gas regulator pressure Specifies the nebulizer gas pressure as specified on the nominal certificate that was shipped with the nebulizer.
Page 106
Power function Sets by what factor a linearization is applied to the data output. As a standard, the Power Function is set to 1.00. For further information, see Power Function Value (} page 112). Page 106 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
If you perform a gradient, it is recommended to perform blank injections with the desired method gradient profile prior to analysis. This "pre-conditioning" of the column improves the performance of both, the analytical column and the detector. Charged Aerosol Detectors (VH-D20, VF-D20) Page 107 Operating Manual...
Consider also the filter constant when setting the data collection rate. Set the two parameters together in order to optimize the amount of data points collected, and reduce short-term noise, while still maintaining peak height, symmetry, and resolution. See below. Page 108 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
It is recommended to always record the evaporation temperature signal channel for troubleshooting purposes. • If a problem occurs, the information from the signal channel can provide helpful information to identify and eliminate the source for the problem. Charged Aerosol Detectors (VH-D20, VF-D20) Page 109 Operating Manual...
See the Guidelines for selecting the evaporation temperature below. TIP Using performance metrics such as the precision and sensitivity of the detector can help to receive the optimal evaporation temperature. Page 110 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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• Perform screening runs at two evaporation temperature settings. VH-D20 detector: Use a minimum of two evaporation ¨ temperatures. Typically, 35 °C and 40 °C are appropriate if the temperature range is unknown. If required, perform additional tests in a maximum of 5 °C increments.
• Operation 6.10.6 Gas Regulation Mode (VH-D20 Detector only) This section applies only to the VH-D20 detector. The detector provides two gas regulation modes to optimize the efficiency of the nebulizer: Analytical mode and MicroLC mode. Each mode sets a specific nebulizer gas pressure for the analysis, based on the gas pressure specified for the nebulizer from the nebulizer certificate.
• Follow the maintenance schedules to keep the system modules in optimum performance state. • Keep the supplied gas in optimum quality for the detector. Charged Aerosol Detectors (VH-D20, VF-D20) Page 113 Operating Manual...
Make sure that the temperature of the column does not exceed 40 °C. • When resuming operation, let the flow equilibrate and verify that the operating parameters for the other system modules are set as required before proceeding. Page 114 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
(50:50) (for example, 1.0 mL/min for 10 minutes with the standard system) to prevent salt buildup in the fluidics. If the solvents in the device are not miscible with water, use an appropriate intermediate solvent. Charged Aerosol Detectors (VH-D20, VF-D20) Page 115 Operating Manual...
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Turn off the gas flow on the detector. d) Wait until the system pressure is down to zero before you continue. e) Turn off the gas supply to the detector. Page 116 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Turn on the device with the main power switch. 4. Before starting an analysis, let the device equilibrate and be sure that it is ready for operation. See Preparing the Device for Operation (} page 93). Charged Aerosol Detectors (VH-D20, VF-D20) Page 117 Operating Manual...
• Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Charged Aerosol Detectors (VH-D20, VF-D20) Page 119 Operating Manual...
However, it is possible to remove a door if this should ever be required for a specific reason or procedure. If you need to remove a door, follow the related steps in Replacing the Doors (} page 143). Page 120 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
• Stop the pump flow prior to opening the flow path. • Wait until the system pressure is down to zero. • When opening the flow path, wear appropriate protective equipment. Charged Aerosol Detectors (VH-D20, VF-D20) Page 121 Operating Manual...
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To stop pump flow and detector gas flow: Turn off the pump flow first, wait at least 5 minutes and only then turn off the detector gas flow. During the waiting period, the drain pump drains any remaining mobile phase to waste. Page 122 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
¨ Depot Repair • If you need to return the device for depot repair, follow the instructions in Transporting or Shipping the Device (} page 145). See also Consumables and Replacement Parts (} page 177) Charged Aerosol Detectors (VH-D20, VF-D20) Page 123 Operating Manual...
• Use the solvent with the best solubility characteristics for the sample matrix and analyte(s) of the analytical method in question. Page 124 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Parts required • Suitable cleaning detergent (or disinfectant) • Purified water • Lint-free cloths or wipes Charged Aerosol Detectors (VH-D20, VF-D20) Page 125 Operating Manual...
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3. Wipe the cleaned surfaces with purified water to ensure that all cleaning detergent residues have been removed. 4. Wipe the surfaces dry using a soft, lint-free cloth or wipe. Page 126 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Consider resetting these parameters after performing the related maintenance procedure: • FilterChanged If you have replaced the gas filter assembly, consider resetting this counter after replacement of the gas filter assembly. • QualificationDone • ServiceDone Charged Aerosol Detectors (VH-D20, VF-D20) Page 127 Operating Manual...
Wait until the system pressure is down to zero before you continue. d) Turn off the gas supply to the detector. 3. Turn off the detector with the main power switch. Page 128 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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On the gas port on the detector, disconnect the elbow connectors of the gas tubes from the port. To re-install the gas tubes or install new gas tubes, follow the steps in Connecting the Gas Inlet and Exhaust Tubes (} page 68). Charged Aerosol Detectors (VH-D20, VF-D20) Page 129 Operating Manual...
If no new waste line is to be installed Close the waste outlet with the seal plug. If the waste line is to be replaced Follow the steps in Connecting the Waste Line (} page 75). Page 130 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
The nebulizer is highly sensitive to contamination. Even minute particles on the nebulizer can lead to contamination and poor detector performance. Always wear powder-free gloves when you install or remove the nebulizer. Charged Aerosol Detectors (VH-D20, VF-D20) Page 131 Operating Manual...
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Mind the correct orientation of the nebulizer when it is unlocked: Figure 26: Unlocking the nebulizer Description Gripping sides of the nebulizer on the top and bottom handle side Protruding attachment locks of the nebulizer on the left and right handle side Page 132 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Figure 28: Nebulizer with protective tubing and plug on the flow inlet c) Pack the nebulizer in its original packaging that it was shipped in. To re-install the nebulizer, follow the instructions in Installing the Nebulizer (} page 62). Charged Aerosol Detectors (VH-D20, VF-D20) Page 133 Operating Manual...
When Interval for gas filter replacement has expired Parts required Gas filter assembly consisting of a charcoal gas filter and a HEPA gas filter Tools required Screwdriver, Torx T10 Page 134 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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1. With the screwdriver, loosen and remove the top screw on the protective cover for the gas filter assembly. Figure 29: Gas filter assembly with protective cover Description Top screw on protective cover Protective cover Bottom screws (slightly loosened) Gas filters Charged Aerosol Detectors (VH-D20, VF-D20) Page 135 Operating Manual...
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(no. 2 in the image below). Figure 30: Removing the protective cover The gas filters are uncovered now: Figure 31: Gas filter assembly Description Charcoal gas filter Elbow fittings HEPA gas filter Gas filter clamps Page 136 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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(no. 2 in the image below). Figure 32: Unlocking an elbow fitting 5. Pull the gas filter assembly out of the clamps. Figure 33: Pulling the gas filter assembly out of the clamps Charged Aerosol Detectors (VH-D20, VF-D20) Page 137 Operating Manual...
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11. Turn on the detector with its main power switch. 12. In Chromeleon, consider updating the Predictive Performance information for the gas filter assembly. 13. Turn on the gas flow. See Turning On/Off the Detector Gas Flow (} page 102). Page 138 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Do not use repaired fuses and do not short-circuit the fuse holders. Follow these steps The fuse holder is located next to the main power switch. Figure 35: Fuse holder Description Main power switch (on/off control) Fuse holder Power-inlet connector Charged Aerosol Detectors (VH-D20, VF-D20) Page 139 Operating Manual...
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Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the device. 5. Turn on the device with the main power switch. Page 140 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Chromeleon software. If the firmware installed in the device is a later version than the version in the Chromeleon software, cancel the download. Charged Aerosol Detectors (VH-D20, VF-D20) Page 141 Operating Manual...
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Requalification of the device may be required. Refer to the release notes. Firmware update failed Turn the device off and on again. Repeat the firmware update. Firmware update fails Contact Thermo Fisher Scientific Technical Support. repeatedly Page 142 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
You can remove the door only when the hinges are in the grooves. Figure 36: Unhinging a door Description Hinge on the housing Reception groove on the door Charged Aerosol Detectors (VH-D20, VF-D20) Page 143 Operating Manual...
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5. Insert the hinges in the groove, by pushing up and slightly turning the door. 6. Push the door downward to lock it in place. You can close the door only when it is properly installed. Page 144 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
3. Remove all cables and flow connections to other devices. 4. Disconnect the gas inlet and exhaust tubes from the detector. See Disconnecting the Gas Inlet and Exhaust Tubes (} page 128). Charged Aerosol Detectors (VH-D20, VF-D20) Page 145 Operating Manual...
Prepare the device for operation (see Preparing the Device for Operation (} page 93)). 5. Before starting an analysis, let the device equilibrate and be sure that it is ready for operation. Page 146 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Thermo Fisher Scientific support organization for the appropriate procedure. Restarting the Device after Shipping To install the device after shipping, follow the instructions on mounting the system stack in the Vanquish System Operating Manual. Charged Aerosol Detectors (VH-D20, VF-D20) Page 147 Operating Manual...
A team effort is in particular required when lifting the device into the system stack or when removing it. Tools required Screwdriver, Torx T20 Preparations 1. Prepare the device for transport. See Transporting or Shipping the Device (} page 145). Page 148 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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The slide-in module can fall down when pulling it out of the enclosure too far. Pull out the slide-in module just far enough so that you can grasp it on both sides from below. Charged Aerosol Detectors (VH-D20, VF-D20) Page 149 Operating Manual...
2. Follow the instructions in Shipping the Device (} page 147). NOTICE Shipping the slide-in module improperly leads to damage to the device. Always ship the slide-in module as described in this operating manual. Page 150 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
2. On the slide-in module, check that you can move the captive screws back and forth with your fingers. If you cannot, screw them in until you can. Charged Aerosol Detectors (VH-D20, VF-D20) Page 151 Operating Manual...
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• If the screws move, tighten the screws further. With a torque wrench, the recommended torque is 1.2 Nm. 8. Set up and restart the device. Page 152 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
2. Prepare all other modules of the Vanquish system for operation and restart them. Refer to the Operating Manuals for the modules. 3. Before starting an analysis, let the chromatography system equilibrate and be sure that it is ready for operation. Charged Aerosol Detectors (VH-D20, VF-D20) Page 153 Operating Manual...
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• Maintenance and Service Page 154 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
• Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device. Charged Aerosol Detectors (VH-D20, VF-D20) Page 155 Operating Manual...
Chromeleon 7 Console Data view, in the folder of the instrument. Messages in the Instrument Audit Trail may be preceded by an icon. The icon identifies the seriousness of the problem. For possible causes and remedial actions, see Messages (} page 158). Page 156 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Send the Instrument Audit Trail as .cmbx file to the Technical Support before you clear the log. • To clear the exception log and continue operation of the module, perform the command ExceptionLogClear. For more information, refer to the Chromeleon Help. Charged Aerosol Detectors (VH-D20, VF-D20) Page 157 Operating Manual...
Find and eliminate the source for the leakage (see Resolving Liquid Leaks (} page 163)). Code 36 Download failed. The firmware download has not been successful. Verify that the correct firmware file was selected. Repeat the download. Page 158 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Page 159
Check the system interlink connections to the module. Verify that all system interlink cables are connected at both ends. 4. If the message persists, replace the system interlink cables. Charged Aerosol Detectors (VH-D20, VF-D20) Page 159 Operating Manual...
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The drain pump remains turned on for a short period longer and is then turned off. Turn the detector off and on. If the message appears again, contact Technical Support. Page 160 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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The allowed interval for the next gas filter replacement will expire XX days remaining. after the stated amount of days. Consider replacing the gas filter assembly. See Replacing the Gas Filter Assembly (} page 134). Charged Aerosol Detectors (VH-D20, VF-D20) Page 161 Operating Manual...
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Check the USB connection. Use only the USB cables provided by detector to PC – check USB Thermo Fisher Scientific for the detector. Avoid CPU-intensive and connection. time-consuming operations on the PC during high-speed data acquisition. Page 162 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Be careful not to bend the sensor. 3. Allow the sensor to adjust to the ambient temperature for a few minutes. 4. If leakage is no longer reported, you can resume operation. Charged Aerosol Detectors (VH-D20, VF-D20) Page 163 Operating Manual...
Wait until the system pressure is down to zero before you continue. d) Turn off the gas supply to the detector. 2. Check that the gas supply pressure is within the allowed range. For the allowed range, see Performance Specifications (} page 170). Page 164 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
If the flooding is minor, the liquid may evaporate when leaving ¨ on gas flow overnight. If the flooding is major, leave the gas flow turned on for ¨ 24 hours. Charged Aerosol Detectors (VH-D20, VF-D20) Page 165 Operating Manual...
Check that the gas supply pressure is stable. If the instability remains, contact Technical Support. Unstable evaporation If the Evap_Tube_Temp signal channel is unstable, this can lead to temperature higher baseline noise and/or poor precision. Contact Technical Support. Page 166 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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Gas leaking Check the gas connections and the gas inlet and exhaust tubes. Remedy the gas leak and/or replace the gas tubes (see Disconnecting the Gas Inlet and Exhaust Tubes (} page 128)). Charged Aerosol Detectors (VH-D20, VF-D20) Page 167 Operating Manual...
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Flooding can occur if the detector is turned off while liquid is still detector (pump flow flowing into the detector. turned on while no gas Resolving Flooding In the Detector (} page 165). flow present) Page 168 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
• Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the device. Charged Aerosol Detectors (VH-D20, VF-D20) Page 169 Operating Manual...
Materials in the flow path Stainless steel (type 316), SimRiz™ NOTICE For information about the chemical resistance of materials refer to the technical literature. Solvent and additive Solvent and Additive Information (} page 27). information Page 170 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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This includes monitoring of the replacement interval for the gas filter assembly and the service interval. All system parameters are logged in the Chromeleon Audit Trail. Charged Aerosol Detectors (VH-D20, VF-D20) Page 171 Operating Manual...
This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts. Charged Aerosol Detectors (VH-D20, VF-D20) Page 173 Operating Manual...
Accessories, consumables, and replacement parts are always maintained at the latest technical standard. Therefore, part numbers are subject to change. If not otherwise stated, updated parts will be compatible with the parts they replace. Page 174 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Viper Capillary, I.D. x length 0.1 mm x 350 mm System interlink cable USB cable, USB 2.0, high-speed, type A to type B, 1 m Tubing bracket For reordering information, see Consumables and Replacement Parts (} page 177). Charged Aerosol Detectors (VH-D20, VF-D20) Page 175 Operating Manual...
Analog signal output kit Contact Service. Includes all components required for installation of an analog signal output to the detector. Installation must be performed by a Thermo Fisher Scientific service engineer. Page 176 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
USB cable, type A to type B, high-speed, USB 2.0 6911.0002A Cable length: 5 m Power cords Description Part No. Power cord, Australia 6000.1060 Power cord, China 6000.1080 Power cord, Denmark 6000.1070 Power cord, EU 6000.1000 Charged Aerosol Detectors (VH-D20, VF-D20) Page 177 Operating Manual...
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Accessories, Consumables and Replacement Parts Description Part No. Power cord, India, SA 6000.1090 Power cord, Italy 6000.1040 Power cord, Japan 6000.1050 Power cord, UK 6000.1020 Power cord, USA 6000.1001 Power cord, Switzerland 6000.1030 Page 178 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
(EFUP) of the item. During this period, the item (when used as intended) will not cause serious damage to human health or environment. For more information, go to http://www.thermofisher.com/us/en/ home/technical-resources/rohs-certificates.html Page 180 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
Operation of this equipment in a residential area is likely to cause harmful interference, in which case the user will be required to correct the interference at his expense. Charged Aerosol Detectors (VH-D20, VF-D20) Page 181 Operating Manual...
Revision Covering VH-D20, VF-D20 VH-D20, VF-D20 2.0a VH-D20, VF-D20 VH-D20, VF-D20 VH-D20 The instructions were prepared in English (original instructions). Other language versions are translations based on the English original instructions. Page 182 Charged Aerosol Detectors (VH-D20, VF-D20) Operating Manual...
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