Thermo Scientific Vanquish VC-D40 Operating Manual
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Thermo Scientific Vanquish VC-D40 Operating Manual

Variable wavelength detectors
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thermo scientific
Vanquish
Variable Wavelength
Detectors
VC-D40, VF-D40
Operating Manual
4820.7701-EN Revision 2.0
•
December 2019

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Summary of Contents for Thermo Scientific Thermo Scientific Vanquish VC-D40

  • Page 1 Vanquish Variable Wavelength Detectors VC-D40, VF-D40 Operating Manual 4820.7701-EN Revision 2.0 • December 2019...
  • Page 2 Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual The hardware descriptions in this manual revision refer to devices VC-D40-A, VF-D40-A. Trademarks Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated. Microsoft and Windows are trademarks of Microsoft Corporation. MP35N is a trademark of SPS Technologies.
  • Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermofisher.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
  • Page 4 Contacting Us Page 4 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 5: Table Of Contents

    Contents Contents 1 Using this Manual .............. 11 About this Manual .................... 12 Conventions...................... 13 1.2.1 Safety Messages.................. 13 1.2.2 Special Notices and Informational Notes .......... 13 1.2.3 Typographical Conventions .............. 14 Reference Documentation ................... 15 2 Safety.................. 17 Safety Symbols and Signal Words................. 18 2.1.1 Safety Symbols and Signal Words in This Manual ........
  • Page 6 Contents Leak Detection...................... 39 Operation ...................... 40 4 Unpacking ..................  41 Unpacking...................... 42 Scope of Delivery.................... 44 5 Installation.................  45 Safety Guidelines for Installation ................. 46 Installing the Device ..................... 47 Site Requirements .................... 49 5.3.1 Power Considerations ................ 49 5.3.2 Power Cord.................... 49 5.3.3 Condensation ...................  50 Accessing the Interior Components .............. 51 Setting Up the Hardware.................. 52 5.5.1...
  • Page 7 Contents Control Elements .................... 78 6.3.1 Keypad......................  78 6.3.2 Status Indicators.................. 79 Power On/Off Control .................. 81 Preparing the Device for Operation .............. 82 6.5.1 Guidelines for Use of Flow Cells ...............  84 6.5.2 Turning On the Lamps ................ 85 Operational Modes of the Device................. 86 6.6.1 Single-Channel Mode ................
  • Page 8 Contents Performing a Wavelength Validation and Calibration........ 119 Replacing the Waste Line ..................  121 Replacing the Main Power Fuses...............  122 7.10 Updating the Device Firmware................ 124 7.11 Replacing the Doors...................  126 7.12 Transporting or Shipping the Device .............. 128 7.12.1 Preparing the Device for Transport............ 128 7.12.2 Transporting the Device to a New Location ...........
  • Page 9 Contents 11.1.3 FCC Compliance.................. 163 11.1.4 NIST Compliance .................. 163 11.1.5 Manual Release History................ 163 11.2 UV Cutoff Wavelengths of Solvents.............. 165 11.3 Digital I/O ...................... 166 Index.................. 169 Variable Wavelength Detectors (VC-D40, VF-D40) Page 9 Operating Manual...
  • Page 10 Contents Page 10 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 11: Using This Manual

    • Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Variable Wavelength Detectors (VC-D40, VF-D40) Page 11 Operating Manual...

  • Page 12: About This Manual

    • Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ device and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. The layout of this manual is designed to provide quick reference to the sections of interest to the user.

  • Page 13: Conventions

    • Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.

  • Page 14: Typographical Conventions

    • Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output • The following appears in bold type: Input that you enter by the keyboard or that you select with the ¨...

  • Page 15: Reference Documentation

    • Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system A printed version of the manual is shipped with the device. •...
  • Page 16 • Using this Manual TIP The Chromeleon Help and documents are included in the software shipment. Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Page 16 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 17: Safety

    • Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device. Variable Wavelength Detectors (VC-D40, VF-D40) Page 17 Operating Manual...

  • Page 18: Safety Symbols And Signal Words

    • Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in This Manual This manual contains safety messages to prevent injury of the persons using the device. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.

  • Page 19: Safety Symbols On The Device

    • Safety 2.1.3 Safety Symbols on the Device The table lists the safety symbols that appear on the device or on labels affixed to the device. Follow the safety notices in this manual to prevent the risk of operator injury or damage to the device. Symbol Description Indicates a potential hazard.

  • Page 20: Intended Use

    • Safety Intended Use The device is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The device is for use by qualified personnel and in laboratory environment only.

  • Page 21: Safety Precautions

    • Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the device. If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired.

  • Page 22: Personal Protective Equipment

    • Safety Installation Only skilled personnel are permitted to install the device and to establish the electrical connections according to the appropriate regulations. • Thermo Fisher Scientific recommends always having service personnel certified by Thermo Fisher Scientific perform the installation (for brevity, referred to as Thermo Fisher Scientific service engineer).

  • Page 23: Electrical Safety Precautions

    • Safety Gloves To protect you from harmful liquids and avoid personal injury during maintenance or service, put on appropriate protective gloves. 2.3.4 Electrical Safety Precautions WARNING—Electric Shock or Damage to the Device High voltages are present inside the device that could cause an electric shock or damage to the device.
  • Page 24 • Safety WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. • Be sure that you know the properties of all substances that you are using.
  • Page 25 • Safety WARNING—Hazardous Vapors Mobile phases and samples might contain volatile or flammable solvents. The handling of these substances can pose health and safety risks. • Avoid accumulation of these substances. Make sure that the installation site is well ventilated. •...

  • Page 26: In Case Of Emergency

    • Safety 2.3.6 In Case of Emergency WARNING—Safety Hazard In case of emergency, disconnect the device from the power line. Page 26 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 27: Solvent And Additive Information

    • Safety Solvent and Additive Information 2.4.1 General Compatibility To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. •...

  • Page 28: Allowed Ph Ranges

    • Safety 2.4.2 Allowed pH Ranges Allowed pH ranges (standard system configuration): System Allowed Remarks (Standard pH ranges Configuration) Vanquish Core 1-13 • pH values of 2 or less: The application time should be as short as possible. Flush the system Vanquish 2-12 thoroughly after these applications.

  • Page 29: Further Information

    • Safety 2.4.4 Further Information • For details about the materials that are used in the analytical flow path of the device, see the Specifications chapter in this manual. For information about the materials that are used in the flow path of the other modules in the Vanquish system, refer to the Specifications chapter in the Operating Manual for the modules.

  • Page 30: Compliance Information

    • Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.

  • Page 31: Device Overview

    • Device Overview 3 Device Overview This chapter introduces you to the device and the main components. Variable Wavelength Detectors (VC-D40, VF-D40) Page 31 Operating Manual...

  • Page 32: Detector Features

    • Device Overview Detector Features The detector comprises the following main features: • The detector is a dual-beam, variable wavelength photometer with one measurement and one internal reference beam. • Two different light sources provide a wavelength detection range from 190 nm to 750 nm (VC-D40) and from 190 nm to 900 nm (VF- D40): A deuterium lamp (referred to as UV lamp) for ultraviolet and ¨...

  • Page 33: Operating Principle

    • Device Overview Operating Principle The detector is a dual-beam spectrophotometer that is designed for ultraviolet (UV), visible (VIS) and near-infrared (NIR) absorption spectrophotometry in combination with HPLC or UHPLC separations. Photometric detection bases upon the absorption of monochromatic light. The optical system of the detector provides a deuterium lamp (UV), and may provide a tungsten lamp (VIS), which is optional for VC- D40 detectors.
  • Page 34 • Device Overview Figure 1: Operating principle of the optics Description VIS lamp (tungsten lamp) Mirror (VIS) UV lamp (deuterium lamp) Mirror (combined light) Shutter Entrance slit Mirror Grating Mirror Beamsplitter Reference photodiode Safety shutter Flow cell Measurement photodiode Page 34 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 35: Interior Components

    • Device Overview Interior Components The user-accessible components of the device are located directly behind the front doors: Figure 2: Interior components (here with flow cell with connection unit installed) Description Cooling air intake Keypad with status indicators Screw holes for connection unit (left-side installation) Flow cell with connection unit Connection unit Leak tray...

  • Page 36: Flow Cell

    • Device Overview Flow Cell The detector design allows easy access to the flow cell on the interior front. Figure 3: Flow cell (example) Description Flow cell screws Flow cell handle Drain outlet Connector for outlet capillary Connection unit screw Connector for inlet capillary Flow Cell Label One or more flow cell labels are present on the flow cell, which contain information such as flow cell type, part number, and serial number.
  • Page 37 • Device Overview The heat exchanger helps to adapt the temperature of the mobile phase to the flow cell temperature before the mobile phase enters the optical flow path within the flow cell. Note that the volume of the heat exchanger and inlet capillary influences the retention times and peak widths.

  • Page 38: Lamps

    • Device Overview Lamps Lamp Types Two different light sources provide the light for a wavelength detection range from 190 nm to 750 nm (VC-D40), or from 190 nm to 900 nm (VF- D40). Note that the VIS lamp is optional for VC-D40. Refer to the table below to find out which lamp is recommended if at least one of your detection wavelengths is within the range indicated in the left column.

  • Page 39: Leak Detection

    • Device Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the device monitors the device for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.

  • Page 40: Operation

    Operating Manual. Details on control and operation of the device are available in the Chromeleon Help. TIP The device can be operated also with other data systems, such as Thermo Scientific Xcalibur . In this case, installation of additional software is required in addition to the data system software. For details, contact the Thermo Fisher Scientific sales organization.

  • Page 41: Unpacking

    • Unpacking 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery. Variable Wavelength Detectors (VC-D40, VF-D40) Page 41 Operating Manual...

  • Page 42: Unpacking

    • Unpacking Unpacking Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the device for any signs of mechanical damage that might have occurred during shipment. If you suspect that the device may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
  • Page 43 • Unpacking Figure 4: Carrying handles on the device Component Carrying handles Attachment screw (one on each carrying handle) 4. Place the device on a stable surface. 5. If applicable: Remove any additional packing material. Leave any protective films attached to the surfaces of the device until it is properly positioned in the system stack.

  • Page 44: Scope Of Delivery

    • Unpacking Scope of Delivery The following items are included in the delivery: • Detector • Ship Kit • Operating manual • Power cord For information on contents of the ship kit or reordering parts, see Accessories, Consumables and Replacement Parts (} page 155).

  • Page 45: Installation

    • Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the device in the Vanquish system and in the chromatography software. Variable Wavelength Detectors (VC-D40, VF-D40) Page 45 Operating Manual...

  • Page 46: Safety Guidelines For Installation

    • Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...

  • Page 47: Installing The Device

    • Installation Installing the Device The Vanquish system is installed and set up by a Thermo Fisher Scientific service engineer, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified.
  • Page 48 • Installation NOTICE If the device is operated with another data system, refer to the documentation for the software that you are using and/or perform the qualification manually. The Instruments Installation Qualification Operating Instructions provide information about the parameters to be adapted and the required settings.

  • Page 49: Site Requirements

    • Installation Site Requirements The operating environment is important to ensure optimal performance of the device. This section provides important requirements for the installation site. Note the following: • Operate the device only under appropriate laboratory conditions. • The device is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.

  • Page 50: Condensation

    • Installation WARNING—Electric Shock or Damage to the Device • Never use a power cord other than the power cords provided by Thermo Fisher Scientific for the device. • Only use a power cord that is designed for the country in which you use the device.

  • Page 51: Accessing The Interior Components

    • Installation Accessing the Interior Components To access the interior components in the device, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the device are located directly behind the doors. Figure 6: Opening the front doors Variable Wavelength Detectors (VC-D40, VF-D40) Page 51...

  • Page 52: Setting Up The Hardware

    • Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the device connectors and cables. 5.5.1 System Arrangement The device is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.

  • Page 53: Connecting The Device

    • Installation For instructions on how to set up the system stack, refer to the Vanquish System Operating Manual. 5.5.2 Connecting the Device Device Connectors The following connectors are provided on the device: Figure 8: Electrical connectors on the right side of the detector Description Rating plate, indicating the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's...
  • Page 54 • Installation Description System Interlink port Allows power on/off control for the detector from the Vanquish system base and device communication and synchronization between the detector and other modules in the Vanquish system. For example, the interconnection between autosampler and detector automatically enables direct synchronization of sample inject and data acquisition start in the detector.

  • Page 55: Connecting The Power Cord

    • Installation 5.5.3 Connecting the Power Cord NOTICE Condensation in a device can damage the electronics. • Before connecting the devices to the power line, be sure that no condensation is present in the devices. • If you suspect that condensation is present, allow the device to warm up to room temperature slowly.

  • Page 56: Installing A Vis Lamp (Tungsten Lamp) (Optional)

    • Installation Installing a VIS Lamp (Tungsten Lamp) (Optional) Follow the instructions below to install the optional VIS lamp. Parts required VIS lamp Preparations 1. Turn off the device. Follow these steps Figure 9: VIS lamp Description VIS lamp locating pin VIS lamp VIS lamp attachment screw VIS lamp connector...
  • Page 57 • Installation Figure 10: Screws on the lamp house cover 3. Remove the lamp house cover. 4. Loosen the 2 screws of the VIS slot protection plate and remove the plate. Keep the VIS slot protection plate for later use. 5. Align the new lamp with the locating pin. When the lamp is in the correct position, push the lamp gently into the lamp house.
  • Page 58 • Installation 12. After installing a new lamp, increased noise and strong baseline fluctuations may occur. Before beginning an analysis or performing a wavelength validation, allow the new lamp to run until the noise is reduced and the baseline is stable which typically takes 24 hours. 13.

  • Page 59: Installing A Flow Cell

    • Installation Installing a Flow Cell This section describes the installation of the flow cell in the detector. • To remove the diagnostic cell, see Removing the Diagnostic Cell (} page 59). • To install a flow cell without connection unit, see Installing a Flow Cell without Connection Unit (} page 60).

  • Page 60: Installing A Flow Cell Without Connection Unit

    • Installation TIP Store the flow cell in a clean place for later use in diagnostic procedures or for transporting the device. 5.7.2 Installing a Flow Cell without Connection Unit Parts required Flow cell, as required by the application Preparations 1.
  • Page 61 • Installation Figure 11: Installing a flow cell with connection unit Description Connection unit Locating pin Connection unit socket Connection unit screw (knurl) 3. Tighten the connection unit screw hand-tight. 4. Push the flow cell completely into the flow cell opening. 5.

  • Page 62: Setting Up The Flow Connections

    • Installation Setting Up the Flow Connections 5.8.1 General Information and Guidelines When setting up flow connections, follow these rules and recommendations: Flow connections can be filled with hazardous substances. Observe the warning messages and precautionary statements presented in Safety Precautions (} page 21).

  • Page 63: Guiding Capillaries And Tubing Through The System

    • Installation NOTICE Flow cells are highly sensitive to contamination, clogging and high backpressures. Even if the pressure exceeds the upper limit for a very short time only, the flow cell may be permanently damaged. Observe the following notes when connecting the flow cell to the system flow path: •...
  • Page 64 • Installation The tubing chase provides four tubing guides. Each guide can hold up to three tubes or lines. In each module, push the tube (or line) into the appropriate guide. Figure 12: Tubing chase with tubing guides (left: view from inside, right: view from top) Use for Solvent tubing (up to three solvent lines)

  • Page 65: Installing The Partition Panel Plugs

    • Installation Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules. Route flow connections from one module to the next module in the Vanquish system through the appropriate guide hole or capillary clip when instructed to do so in the manual.

  • Page 66: Connecting Fittings, Capillaries, And Tubing

    • Installation 3. To secure the capillary, push the capillary into the slit of the plug. Figure 16: Capillary installed in the plug with slit Installing the rotating plug 1. On the detector partition panel, push the rotating plug in the required recess of the partition panel (if not present yet).
  • Page 67 • Installation 5.8.4.1 General Guidelines When connecting capillaries and tubing, follow these general recommendations: • Use only the capillaries and tubing (for example, solvent lines or waste tubing) that are shipped with the product or additional or spare capillaries and tubing as recommended by Thermo Fisher Scientific.

  • Page 68: Flow Connections To The Flow Cell

    • Installation Figure 18: Viper fitting with knurl Description Knurl Capillary Slot 1. Insert the Viper capillary into the connection port. 2. Tighten the connection by the knurl. TIP Note the slot in the knurl. For narrow connections, you can easily remove the knurls from neighboring capillaries through this slot and attach them again later.
  • Page 69 • Installation In addition, observe the guidelines for proper connection of the flow cell General Information and Guidelines (} page 62). Parts required • Inlet capillary • Detector waste line For instructions on connecting the waste line, follow the steps in Connecting the Detector Waste Line (} page 71).
  • Page 70 • Installation 5.8.5.1 Connecting the Inlet Capillary Preparations Flow Connections to the Flow Cell (} page 68). Follow these steps Figure 19: Connecting the inlet capillary from the column compartment (example) 1. Route the inlet capillary from the column compartment through the guide hole in the device enclosure. Use the guide hole that is next to the column compartment.
  • Page 71 • Installation 5.8.5.2 Connecting the Detector Waste Line Preparations 1. See Flow Connections to the Flow Cell (} page 68). Follow these steps Figure 20: Connecting the detector waste line 1. Connect the waste line to the flow cell outlet or to the outlet (OUT) on the flow cell connection unit (1).

  • Page 72: Guiding Liquid Leaks To Waste

    • Installation 6. Check the waste line over the entire flow path: Make sure that no bending (kink), pinching or squeezing of the waste line is present at any point in the flow path. 5.8.6 Guiding Liquid Leaks to Waste Leaking liquids of the device are collected in the leak tray, where they flow off through the chase on the right side of the leak tray to the drain system.

  • Page 73: Turning On The Device

    • Installation Turning On the Device Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...

  • Page 74: Setting Up The Device In The Software

    • Installation 5.10 Setting Up the Device in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.

  • Page 75: Operation

    • Operation 6 Operation This chapter describes the elements for device control, provides information for routine operation and for shutdown. Variable Wavelength Detectors (VC-D40, VF-D40) Page 75 Operating Manual...

  • Page 76: Introduction To This Chapter

    • Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the device has already been completed. If this is not the case, refer to the instructions in Installation (} page 45). For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.

  • Page 77: Safety Guidelines For Operation

    • Operation Safety Guidelines for Operation When operating the device, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Hot Surfaces Surfaces inside the device may become hot during operation. Touching hot parts might cause burns.

  • Page 78: Control Elements

    • Operation Control Elements The device is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the device: • Keypad The keypad buttons allow you to perform certain functions directly from the device.

  • Page 79: Status Indicators

    • Operation MUTE ALARM Beeping alerts you when the device detects a problem, for example, leakage. To turn off the beep for the current alarm, press this button. Eliminate the source for the alarm within 10 minutes. Otherwise, beeping starts again. If the device detects a different problem, beeping alerts you again immediately.
  • Page 80 • Operation LED Bar The LED bar provides the information when the device is closed. When the device is connected in the Chromeleon software, the LED bar may provide less information. LED Bar Description Off (dark) The power to the device is turned off. Dimmed The doors of the device are open.

  • Page 81: Power On/Off Control

    • Operation Power On/Off Control The power switch on the device is the main switch for power on/off control. The main power switch is turned on during initial installation of the device. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.

  • Page 82: Preparing The Device For Operation

    • Operation Preparing the Device for Operation This section gives information on any additional steps that are required to prepare the device for operation and sample analysis. Before Operating the Device for the First Time Prepare the device for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the device for the first time:...
  • Page 83 • Operation System Equilibration System equilibration should include the following operations: • Purging the pump (all channels, including those not used for the application) • Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run •...

  • Page 84: Guidelines For Use Of Flow Cells

    • Operation 6.5.1 Guidelines for Use of Flow Cells NOTICE Flow cells are sensitive to damage and contamination. • Handle flow cells with care. See Guidelines for Handling Flow Cells (} page 115). • Observe the guidelines below when operating the detector. Operating conditions Observe the specified maximum pressure limit for the flow cell.

  • Page 85: Turning On The Lamps

    • Operation 6.5.2 Turning On the Lamps You can turn on both lamps using the UV and VIS buttons on the keypad of the device or using the Chromeleon software. It is recommended to turn on the UV lamp prior to the VIS lamp. TIP To ignite the lamps, the lamp house cover must be installed.

  • Page 86: Operational Modes Of The Device

    • Operation Operational Modes of the Device 6.6.1 Single-Channel Mode Single-channel mode is the default mode of operation for the detector. As this mode provides the highest possible data rates and best signal-to- noise ratio, always use the single-channel mode for routine operation of the detector.
  • Page 87 • Operation Consider the following, when operating the detector in multi- wavelength mode: • Each additional channel reduces the maximum possible data collection rate, and leads to an increased baseline noise. • Switching the optical grating permanently between the wavelengths can lead to wear on the mechanical parts of the detector in the long term.

  • Page 88: Important Operating Parameters

    • Operation Important Operating Parameters If a parameter listed below is not available in the Chromeleon software, consider updating the firmware and Chromeleon version. TIP The Instrument Method Wizard provides different parameter view modes. Depending on the parameters to be set, you can select the desired view mode (for example, Easy or Advanced).
  • Page 89 • Operation Parameters Description Spectra scanning To record the absorbance spectra of substances that are used in an analysis, for example, to detect peak impurities, or to identify optimum wavelengths for method development, perform spectra scanning: ScanStartWavelength sets the wavelength at which the scan should start.

  • Page 90: Optimizing The Performance Of The Device

    • Operation Optimizing the Performance of the Device This section provides information for best performance of the device and gives hints on what you can do to optimize the performance further. 6.8.1 General Guidelines Consider the following guidelines for optimization of the device performance: •...

  • Page 91: Overview Of Optimization Parameters

    • Operation 6.8.2 Overview of Optimization Parameters The following table serves as an overview of parameters that influence the detector performance and indicates the performance characteristics affected. Parameter Affects Wavelength Sensitivity, linearity Data collection rate Peak resolution, disk space Response Time/Peak width Baseline noise, peak width, chromatographic resolution, sensitivity Baseline behavior...

  • Page 92: Data Collection Rate

    • Operation 6.8.4 Data Collection Rate The data collection rate is the number of data points per second (Hz) that the Chromeleon software collects from the detector and stores as raw data. When you select a data collection rate, observe the following guidelines: •...

  • Page 93: Baseline Behavior

    • Operation • The response time should be about 30% of the peak width at half- height of the narrowest peak of interest. • A longer response time allows more averaging of the signal and results in less short-term noise. However, if the selected response time is too long, this can result in reduced peak heights and asymmetrical peak shapes.

  • Page 94: Shutting Down The Device

    • Operation Shutting Down the Device If the device will not be operated for some time, follow the instructions in this section to shut down the device. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.

  • Page 95: Long-Term Shutdown

    • Operation 6.9.2 Long-Term Shutdown Shutting Down the Device To interrupt operation for a longer period, follow the instructions below. TIP Shutting down the device affects the operation of the system. When shutting down the device, also observe the shutting down instructions for the other Vanquish system modules and take appropriate action (refer to the Operating Manuals for the modules).
  • Page 96 • Operation Situation after Shutdown If no additive is used If an additive is used Device and flow cell shall Flush the system with Flush the system first with be transported or shipped isopropanol. several volumes of after shutdown methanol and water (50:50) (for example, 1.0 mL/min for 10 minutes with the...
  • Page 97 • Operation Situation Steps Device shall be transported or shipped If one of the modules shall be removed after shutdown from the system stack, turn off all system modules with their main power switch. Pressing the system power button will not be sufficient to turn off the power to the devices completely.
  • Page 98 • Operation Page 98 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 99: Maintenance And Service

    • Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Variable Wavelength Detectors (VC-D40, VF-D40) Page 99 Operating Manual...

  • Page 100: Introduction To Maintenance And Service

    • Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).

  • Page 101: Safety Guidelines For Maintenance And Service

    • Maintenance and Service Safety Guidelines for Maintenance and Service When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). WARNING—High Voltage High voltages are present inside the device that could cause an electric shock.
  • Page 102 • Maintenance and Service CAUTION—Hot Surfaces Surfaces inside the device may become hot during operation. Touching hot parts might cause burns. Allow hot surfaces to cool down before starting replacement or maintenance procedures. CAUTION—Hydrostatic Pressure Solvent may spill when you open the flow path. This is due to hydrostatic pressure in the system when the solvent reservoirs are located above the pump outlet.

  • Page 103: General Rules For Maintenance And Service

    • Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow these rules and recommendations: • Before starting maintenance or service procedures, shut down the device when instructed to do so. • Use only the replacement parts specifically authorized and qualified for the device by Thermo Fisher Scientific.

  • Page 104: Routine And Preventive Maintenance

    • Maintenance and Service Routine and Preventive Maintenance Optimum device performance, maximum uptime of the device, and accurate results can be obtained only if the device is in good condition and properly maintained. 7.4.1 Maintenance Plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.
  • Page 105 • Maintenance and Service Decontamination Decontamination is required, for example, when leakage or spillage has occurred, or before service or transport of the device. Use a suitable cleaning detergent or disinfectant to ensure that the treatment renders the device safe to handle. Parts required •...

  • Page 106: Predictive Performance

    • Maintenance and Service Preparations 1. Turn off the power to the device and disconnect the power cord from the power source. Follow these steps 1. Wipe the surfaces clean with a clean, dry, soft, lint-free cloth or wipe. If necessary, slightly dampen the cloth or wipe with a solution of lukewarm water and a suitable cleaning detergent.

  • Page 107: Lamps

    • Maintenance and Service Lamps This section describes the replacement and installation of the • UV lamp (see Replacing the UV Lamp (Deuterium Lamp) (} page 107)), and • VIS lamp (see Replacing the VIS Lamp (Tungsten Lamp) (} page 110)). NOTICE—Damage to the device Usage of lamps not supplied or approved by Thermo Fisher Scientific for this detector may result in inferior performance, detector malfunction or may damage the detector.
  • Page 108 • Maintenance and Service Follow these steps Figure 22: UV lamp Description UV lamp connector UV lamp UV lamp attachment screw UV lamp locating pin 1. Open the doors. 2. Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose.
  • Page 109 • Maintenance and Service Figure 23: Screws on the lamp house cover 3. Remove the lamp house cover. CAUTION—Hot surface The lamp may become hot. Touching a hot lamp might cause burns. • Touch the lamp briefly and carefully to find out if it is hot before you remove the lamp.

  • Page 110: Replacing The Vis Lamp (Tungsten Lamp)

    • Maintenance and Service 11. Mind the routing of the lamp cables. Position the lamp cables as indicated in the figure above to prevent the cable from being pinched under the lamp house cover. 12. Reinstall the lamp house cover and fasten the screws on the lamp house cover.
  • Page 111 • Maintenance and Service Follow these steps Figure 24: VIS lamp Description VIS lamp locating pin VIS lamp VIS lamp attachment screw VIS lamp connector 1. Open the doors. 2. Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose.
  • Page 112 • Maintenance and Service Figure 25: Screws on the lamp house cover 3. Remove the lamp house cover. CAUTION—Hot surface The lamp may become hot. Touching a hot lamp might cause burns. • Touch the lamp briefly and carefully to find out if it is hot before you remove the lamp.
  • Page 113 • Maintenance and Service 11. Install the lamp house cover and fasten the screws on the lamp house cover. 12. Turn on the device with its main power switch. 13. If you install a VIS lamp for the first time and the detector is operated with Chromeleon, activate the lamp in Chromeleon: Only then, the VIS lamp can be ignited from Chromeleon.

  • Page 114: Flow Cell

    • Maintenance and Service Flow Cell This section describes cleaning, replacement, storage and shipment of flow cells. To store or ship the flow cell, follow these steps: 1. Flush the flow cell with isopropanol. 2. Remove the flow cell from the detector. See Removing the Flow Cell (} page 116).

  • Page 115: Guidelines For Handling Flow Cells

    • Maintenance and Service 7.6.1 Guidelines for Handling Flow Cells NOTICE Flow cells are highly sensitive to dirt and dust. Observe the following notes when handling flow cells: • When holding flow cells, do not touch the optical block of the flow cell or the sensitive electronics on the flow cell rear side.

  • Page 116: Removing The Flow Cell

    • Maintenance and Service WARNING—Health Risk The handling of solvents can pose health and safety risks. Wear personal protective equipment as required by the hazard and follow good laboratory practice. Refer to the material handling and safety data sheet provided by the vendor. Follow these steps You can perform the following procedure by using the optional flushing and injection kit.
  • Page 117 • Maintenance and Service Follow these steps NOTICE Do not open the tubing connections between the connection unit and the flow cell. The tubing connections are installed at the factory. 1. Flow cell without connection unit only Disconnect the waste line from the outlet of the flow cell, then disconnect the inlet capillary from the inlet of the flow cell (if not yet done).

  • Page 118: Installing A Diagnostic Cell

    • Maintenance and Service 7.6.4 Installing a Diagnostic Cell Parts required Diagnostic cell Follow these steps 1. Push the diagnostic cell into the flow cell opening. 2. Tighten the two screws of the diagnostic cell hand-tight. Page 118 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 119: Performing A Wavelength Validation And Calibration

    • Maintenance and Service Performing a Wavelength Validation and Calibration A holmium-oxide glass filter is used to validate the wavelength accuracy. The filter can be moved into the light path in front of the entrance slit for the wavelength validation. The detector determines the wavelengths of several absorption peaks in the resulting spectrum and compares them to the nominal holmium oxide values that are stored in the detector firmware.
  • Page 120 • Maintenance and Service • Allow the lamps to warm up and reach operating temperature. The lamps should be running for at least 1 hour before you start the wavelength validation. A lamp spectrum changes significantly during the first few minutes after a lamp has been turned on. To perform a wavelength validation 1.

  • Page 121: Replacing The Waste Line

    • Maintenance and Service Replacing the Waste Line When Blockage or leakage of one or more waste line components Parts required Detector waste line For instructions on connecting the waste line, follow the steps in this section. The waste line is routed through the system base and connected to the waste.

  • Page 122: Replacing The Main Power Fuses

    • Maintenance and Service Replacing the Main Power Fuses When Blown fuses Parts required Fuses (2 fuses, 3.15 AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock High voltages are present inside the device that could cause an electric shock or damage to the device.
  • Page 123 • Maintenance and Service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the device. 5.

  • Page 124: Updating The Device Firmware

    • Maintenance and Service 7.10 Updating the Device Firmware When Updating the device firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version. Items required Firmware version/Chromeleon version as appropriate TIP When a new firmware version is released, the new version will be included in the next available Chromeleon version.
  • Page 125 • Maintenance and Service 3. Monitor the Audit Trail of the Instrument Configuration Manager program to see whether the firmware update was successful or failed. If the firmware update failed, turn the device off and on again ¨ and repeat the firmware update. If the firmware update fails repeatedly, contact Thermo Fisher ¨...

  • Page 126: Replacing The Doors

    • Maintenance and Service 7.11 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the related steps in this section. Parts required Replacement door Follow these steps...
  • Page 127 • Maintenance and Service 3. To install the door, align the door with the hinges on the housing. Be careful not to clamp tubing or capillaries between the door and the enclosure. 4. Insert the hinges in the groove, by pushing up and slightly turning the door.

  • Page 128: Transporting Or Shipping The Device

    • Maintenance and Service 7.12 Transporting or Shipping the Device If you want to transport the device to a new location or if you need to ship the device, first prepare the device for transport and then move or ship the device as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely.

  • Page 129: Transporting The Device To A New Location

    • Maintenance and Service NOTICE The flow cell opening on the device is sensitive to dust and debris. If no flow cell is installed to the detector, install a diagnostic cell to the flow cell opening of the detector. 5. Remove the device or slide-in module from the system stack as required: Install the carrying handles and remove the device from the Vanquish system.

  • Page 130: Shipping The Device

    • Maintenance and Service 7.12.3 Shipping the Device Preparations Prepare the device for transport. See Preparing the Device for Transport (} page 128). Follow these steps 1. Follow the unpacking instructions in this manual in the reverse order. Use only the original packing material and shipping container. If the original shipping container is not available, appropriate containers and packing material can be ordered from the Thermo Fisher Scientific sales organization.

  • Page 131: Replacing The Slide-In Module

    • Maintenance and Service 7.13 Replacing the Slide-In Module 7.13.1 Removing the Slide-In Module CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...

  • Page 132: Returning The Slide-In Module

    • Maintenance and Service Figure 29: Pulling out the slide-in module NOTICE The slide-in module can fall down when pulling it out of the enclosure too far. Pull out the slide-in module just far enough so that you can grasp it on both sides from below.

  • Page 133: Installing The Slide-In Module

    • Maintenance and Service NOTICE Shipping the slide-in module improperly leads to damage to the device. Always ship the slide-in module as described in this operating manual. CAUTION—Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel. •...
  • Page 134 • Maintenance and Service Follow these steps 1. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to insert the slide-in module properly into the enclosure in the next step.

  • Page 135: Setting Up The Slide-In Module

    • Maintenance and Service NOTICE • Verify that the screws are tightened. Pull the slide-in module by the leak tray towards the front and check whether the screws move. If they do not move, the slide-in module is installed properly. •...
  • Page 136 • Maintenance and Service Page 136 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 137: Troubleshooting

    • Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device. Variable Wavelength Detectors (VC-D40, VF-D40) Page 137 Operating Manual...

  • Page 138: General Information About Troubleshooting

    • Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the device. TIP For information about operating issues that might occur during the operation of a Vanquish system, refer to the Vanquish System Operating Manual.
  • Page 139 • Troubleshooting Messages in the Chromeleon Audit Trail are preceded by an icon. The icon identifies the seriousness of the problem (refer to the Chromeleon Help). For possible causes and remedial actions, see Messages (} page 140). Firmware Failure If a firmware failure occurred during operation of the module, an exception log has been created about the processes during the firmware failure.

  • Page 140: Messages

    • Troubleshooting Messages The table lists the most frequently observed messages for the device and provides troubleshooting assistance. Each message consists of a code number and a text. The code number is the unique identifier for the problem while the wording may change. Note the following: •...
  • Page 141 • Troubleshooting Message and Code Description and Remedial Action Code 90 Download firmware mismatch – You tried to download a firmware with an earlier version number invalid version. than the firmware that is currently installed in the device. Downgrading the firmware may result in loss of functionality or malfunctioning of the device.
  • Page 142 • Troubleshooting Message and Code Description and Remedial Action Code 7049 Wavelength calibration failed Check the flow cell (see Checking the Flow Cell (} page 145)). If – check and flush flow cell. the message still appears, proceed as follows: Check the lamps: If a lamp was newly installed, ensure that it is installed properly.
  • Page 143 • Troubleshooting Message and Code Description and Remedial Action Code 7066 Invalid data rate/response Select a higher data rate or longer response time for the response time combination. Filter not effective. time filter to be effective. Use the Chromeleon Instrument Method Wizard for valid combinations. Code 7111 Lamp cover not in place - Check that the lamp cover is installed properly.
  • Page 144 • Troubleshooting Message and Code Description and Remedial Action Code 7143 Internal process failed – no The flow cell has been removed while an internal process was flow cell. running. Re-install a flow cell (see Installing a Flow Cell (} page 59)). It may be necessary to repeat the previous command or operation.

  • Page 145: Checking The Flow Cell

    • Troubleshooting Checking the Flow Cell When Related messages that require a flow cell check appear in the Audit Trail. Follow these steps 1. Check that the flow cell is installed properly. 2. Remove and re-install the flow cell. 3. Flush the flow cell with pure HPLC-grade water with a flow rate of >1 mL/min for several minutes (Nano flow cells: Flush with pure HPLC-grade water with the maximum possible flow rate for the respective range for several minutes).

  • Page 146: Resolving Liquid Leaks

    • Troubleshooting Resolving Liquid Leaks When The leak sensor is wet. The leak sensor reports leakage. Parts and additional items required • Replacement part as required • Cloth or tissue Preparations When resolving leakage, observe the safety guidelines and general rules for maintenance and service as presented in Maintenance and Service (} page 99).
  • Page 147 • Troubleshooting Follow the steps depending on the location of the leak: Location of the leak Steps Flow cell inlet and/or outlet 1. Tighten the connection where liquid is visible. 2. If the connection seems tight but is still leaking, remove the connection/fitting and check for damage.
  • Page 148 • Troubleshooting Page 148 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 149: Specifications

    • Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the device. Variable Wavelength Detectors (VC-D40, VF-D40) Page 149 Operating Manual...

  • Page 150: Performance Specifications

    • Specifications Performance Specifications 9.1.1 Detector The device performance is specified as follows: Type Specification Optical design UV/VIS/NIR photometer (tunable Czerny-Turner monochromator) with additional internal reference beam Light source • Deuterium lamp for UV and VIS • Tungsten lamp for VIS and NIR (optional for VC-D40) •...
  • Page 151 • Specifications Type Specification Noise (Multi-channel mode) <± 10.0 µAU (typical: <± 7.0 µAU), wavelengths: 254 nm and 280 nm, only deuterium lamp turned on <± 10.0 µAU (typical: <± 7.0 µAU), wavelengths: 450 nm and 520 nm, only tungsten lamp turned on Standard flow cell with 1 mL/min HPLC-grade water, degassed, column in flow path, time constant: 2 seconds, data collection rate: 1 Hz All values reached within 60 minutes of warm-up (typical).

  • Page 152: Flow Cells

    • Specifications For best performance, minimize the frequency and the amplitude of changes in the ambient temperature to 1 °C/hour. Time constant and response time According to ASTM E1657-98, the time constant is converted to the response time by multiplying by the factor 2.2 (i.e., response time = 2.2 x time constant).

  • Page 153: Physical Specifications

    • Specifications Physical Specifications The physical conditions of the device are specified as follows: Type Specification Range of use Indoor use only Ambient operating 5 °C - 35 °C temperature Ambient storage temperature -20 °C - 45 °C Ambient operating humidity 20% - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing) Operating altitude...
  • Page 154 • Specifications Page 154 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 155: Accessories, Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts.

  • Page 156: General Information

    • Accessories, Consumables and Replacement Parts 10.1 General Information The device must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.

  • Page 157: Ship Kit

    • Accessories, Consumables and Replacement Parts 10.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. See the content list included in the kit for the most recent information about the kit content at the time when the device is shipped.

  • Page 158: Optional Accessories

    • Accessories, Consumables and Replacement Parts 10.3 Optional Accessories Flow cells and Flow cell accessories Item Part No. Standard flow cell, SST, 10 mm 6077.0250 Semi-micro flow cell, SST, 7 mm 6077.0360 Standard Bio flow cell, PEEK, 10 mm 6077.0200 Semi-micro Bio flow cell, PEEK, 7 mm 6077.0300 Nano flow cell, fused silica, 10 mm* 6074.0270...

  • Page 159: Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10.4 Consumables and Replacement Parts Capillaries and tubing Description Part No. Waste line 6036.2425 For system capillaries, refer to the Vanquish System Operating Manual. Lamps Description Part No. Standard UV lamp (deuterium lamp) 6077.1110 High sensitivity UV lamp (deuterium lamp) 6077.1111 VIS lamp (tungsten lamp)
  • Page 160 • Accessories, Consumables and Replacement Parts Description Part No. Power cord, Japan 6000.1050 Power cord, UK 6000.1020 Power cord, USA 6000.1001 Power cord, Switzerland 6000.1030 Page 160 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 161: Appendix

    • Appendix 11 Appendix This chapter provides additional information about compliance, UV cutoff wavelengths, and the use of the digital I/O ports. Variable Wavelength Detectors (VC-D40, VF-D40) Page 161 Operating Manual...

  • Page 162: Compliance Information

    • Appendix 11.1 Compliance Information 11.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. cTUVus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark.

  • Page 163: Weee Compliance

    • Appendix 11.1.2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive. It is marked with the following symbol: Figure 32: WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 164 • Appendix The instructions were prepared in English (original instructions). Other language versions are translations based on the English original instructions. Page 164 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 165: Uv Cutoff Wavelengths Of Solvents

    • Appendix 11.2 UV Cutoff Wavelengths of Solvents The UV cutoff wavelength is the minimum effective wavelength for the measurement. The mobile phase composition affects its UV cutoff wavelength. In general, mobile phases are solvents, such as, water, acetonitrile, methanol, or other substances. They may also contain salts, such as sodium hydroxide (NaOH).

  • Page 166: Digital I/O

    • Appendix 11.3 Digital I/O The digital I/O ports (Dig I/O) can be used to exchange digital signals with external devices. Each port provides: • one digital input • one relay output Pin Assignment Figure 33: Digital I/O port Description Not used Relay output — Relay_NC (Normally Closed contact) Ground — GND Digital input — Input...
  • Page 167 • Appendix Wire Color Signal Name Signal Level Remarks Yellow Digital input — Input (low active): Digital input; reference potential is Input On: 0-0.4 V ground. Note the following: Off: 2.2-5 V • The maximum input voltage at the Pull-up resistor: 47 kΩ to 5 V input must not exceed +5 V with reference to ground.
  • Page 168 • Appendix Page 168 Variable Wavelength Detectors (VC-D40, VF-D40) Operating Manual...

  • Page 169: Index

    Index Index detector waste line .......... 71 deuterium lamp (UV) ....... 38, 107 accessories .......... 44, 155 device optional ............. 158 optimization .......... 90 ship kit............ 157 preparing for operation ...... 82 additives ............ 27 shutdown ............ 94 information .......... 27 diagnostic cell........... 59 audit trail............ 138 Dig I/O ............ 54, 166 Audit Trail messages ........ 140 digital I/O.......... 54, 166 Autozero............ 93 disk space ............ 91...
  • Page 170 Index waste line replacement...... 121 leak detection.......... 39, 88 with connection unit ........ 60 leak sensor ............. 146 fuses ............... 122 leak sensor mode .......... 88 leakage ............ 146 LED bar .......... 78, 79, 138 linearity ............ 91 gloves ............... 23 liquid leaks............ 146 guide hole............ 63 long-term shutdown ........ 95 guidelines flow cell handling ........ 84 flow cell maintenance ....... 115 installation........... 46...
  • Page 171 Index optimization ............. 90 qualification of personnel ...... 21 baseline behavior ........ 93 servicing ............ 101 data collection rate ........ 92 safety symbols.......... 18, 19 parameters overview ........ 91 sensitivity ............ 91 peak width........... 92 service .............. 99 response time .......... 92 setup wavelength.......... 91 hardware............. 52 outlet waste line.......... 71 software ............ 74 overview (functional) ........ 31 system arrangement ........ 52 ship.............. 128 ship kit ............ 44, 157...
  • Page 172 Index transport ............ 128 troubleshooting.......... 137 general information ........ 138 messages........... 140 tubing ............... 67 tubing bracket .......... 63 tubing chase ............. 63 tubing guides............ 63 tungsten lamp (VIS) ........ 38, 107 type label............ 19 Universal Serial Bus .......... 54 unpack.............. 42 USB .............. 54 UV button............ 79 UV cutoff wavelengths ........ 165 UV lamp.......... 38, 89, 107 turn on ............ 85 UV_VIS.............. 89 Viper fitting system .......... 67...
  • Page 173 Index Variable Wavelength Detectors (VC-D40, VF-D40) Page 173 Operating Manual...
  • Page 174 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

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