Equilibrating The System - Thermo Scientific Corona Veo Operating Manual

6.5.3

Equilibrating the System

Charged Aerosol Detectors Corona Veo (RS) Operating Manual
Before you use the detector with the system and start sample analysis,
flush the system thoroughly.
Make sure that the chromatography system is properly equilibrated.
System equilibration should include the following operations:
1. Remove the analytical column from the system flow path.
2. Disconnect the detector from the system flow path.
3. Verify that a starting solution of 80% water and 20% methanol is
miscible with the previously used mobile phase.
♦
If the starting solution is not miscible, flush with an intermediate
solvent.
♦ If the system has been used with non-volatile buffers, extensive
flushing with an intermediate solvent is required.
4. Flush the system with the starting solution of 80% water and 20%
methanol without the detector until the system is free of any other
liquid composition, for example solvent from a previous run.
TIP If you use an online degasser in the system, flush each channel
of the online degasser before you use the channels for sample
analysis with the charged aerosol detector.
5. Pre-program all temperature-controlled devices in the system to the
starting temperature required for the application. Warming up (or
cooling down) the temperature-controlled devices can include, for
example, the column compartment or the sample compartment
thermostatting in the autosampler.
6. Change the solvent to the mobile phase required for the application.
If the mobile phase for application is not miscible with the starting
solution, use an intermediate solvent.
7. Flush the system with the mobile phase for the application until the
system is free of the starting solution (80% water and 20%
methanol). The detector is still disconnected from the system flow
path.
8. Install an analytical column that is dedicated for use with volatile
solvents to the system flow path.
6 • Operation
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