Glow Luminescence - tecan Infinite 200 PRO Instructions For Use Manual

7.3.5

Glow Luminescence

Sensitivity
Measurement Parameters:
Plate Layout:
1 2
A Bx ATP
B Bx ATP
C Bx ATP
D Bx ATP
E
F
G
H
Material/Reagents:
2016-04
For the Infinite 200 PRO with the option 'Luminescence the following tests may
be performed to prove the sensitivity specifications:
Perform the following measurement to determine the sensitivity:
Parameter
Reading Mode
Integration Time
Settle Time
Plate Type
Part of the Plate
Pipette 200 µl of the ATP reagents into the appropriate wells according to the
plate layout:
3 4 5
Bx B B
Bx B B
Bx B B
Bx B B
-8
ATP 2*10 M ATP (final concentration in well)
B Blank (ATP reagent: Tris-EDTA=1:5)
Bx Blank (wells used for cross-talk calculation)
BioThema ATP Kit (ATP-Kit SL 144-041, BioThema AB)
1 Greiner 96-well plate white
200 µl Pipette + tips
IFU for Infinite 200 PRO No. 30052730
Setting
Luminescence
1000 ms
0
GRE96fw
A1 – D10
6 7 8
B B B
B B B
B B B
B B B
Rev. No. 1.6
7. Quality Control
9 10 11
B B
B B
B B
B B
12
169