Defining Filter Slides (Infinite F200 Pro Only); About Filters; Filter Slide And Filter Orientation - tecan Infinite 200 PRO Instructions For Use Manual

5. Operating the Instrument
5.4

Defining Filter Slides (Infinite F200 PRO only)

5.4.1

About Filters

Fluorescence Filters
Absorbance Filters
5.4.2

Filter Slide and Filter Orientation

Filter Slide
78
The optical filters (bandpass style) in a filter slide are specially designed for
fluorescence measurements. The spectral rejection and the bandwidth of the
fluorescence filters are optimized for achieving excellent sensitivity.
Contact TECAN for filters other than those supplied on the delivered filter slides.
Bandpass filters, which are commonly used in microplate readers for absorbance
measurements, usually have a bandwidth of 10 nm. Therefore it is not
recommended to use fluorescence filters for absorbance measurements because
the bandwidth (FWHM) is usually larger than 10 nm. This could cause a bright
value error or low OD values when measuring dyes with narrow peaks.
The Infinite F200 PRO filter slide consists of an excitation and an emission part.
The filter slide enables the user to work with four independent excitation/emission
filter pairs, which can be defined on positions 1 to 4. The information about the
inserted filters is saved on the integrated microchip.
Figure 36: Infinite F200 PRO: Filter slide
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04