tecan infinite 200 Manuals

tecan infinite 200 Manuals

Manuals and User Guides for tecan infinite 200. We have 1 tecan infinite 200 manual available for free PDF download: Instructions For Use Manual

tecan infinite 200 Instructions For Use Manual

tecan infinite 200 Instructions For Use Manual (162 pages)

Brand: tecan | Category: Laboratory Equipment | Size: 2 MB

Table of Contents

Table of Contents
5
1 Safety

9
- Instrument Safety
  9
2 General Description

11
- Instrument
  11
- Intended Use
  11
- Multifunctionality
  12
- Performance
  13
- User Friendliness
  13
- System Requirements
  14
- Measurement Techniques
  15
- Fluorescence
  15
- Absorbance
  17
- Luminescence
  18
- Injectors
  20
- Measurement with Injectors
  21
- Storage Bottles
  21
- Injector/Injector Carrier
  22
- Priming and Washing of the Infinite
  25
- Injector Cleaning and Maintenance
  35
- Injector Reagent Compatibility
  36
- Software
  38
- I-Control and Injectors
  38
- I-Control Examples
  43
3 Installation

47
- Unpacking and Inspection
  47
- Unpacking Procedure
  48
- Removal of the Transport Locks
  49
- Transport and Storage
  51
- Transport
  51
- Storage
  51
- Power Requirements
  51
- Switching the Instrument on
  52
4 Defining Filter Slides (Infinite ® F200 Only)

55
- About Filters
  55
- Fluorescence Filters
  55
- Absorbance Filters
  55
- Filter Slide and Filter Orientation
  55
- Filter Slide
  55
- Filter Types
  56
- Position of Polarization Filters
  57
- Installing a Custom Filter
  58
- Removing a Filter
  58
- Mounting a Custom Filter
  58
- Defining the Filters
  60
5 Optical System

65
- Fluorescence Intensity System - Infinite ® M200
  65
- Light Source System Fluorescence Intensity
  67
- Fluorescence Top/Bottom Optics
  69
- Fluorescence Intensity Detection
  70
- Fluorescence Intensity System - Infinite
  71
- Light Source System
  73
- Fluorescence Optics Top
  74
- Fluorescence Optics Bottom
  74
- Fluorescence Detection
  74
- Fluorescence Polarization System - Infinite ® F200
  75
- Absorbance System - Infinite ® M200
  76
- Absorbance Optics MTP
  77
- Absorbance Detection MTP
  77
- Cuvette Port
  78
- Absorbance Optics Cuvette
  79
- Absorbance Detection Cuvette
  79
- Cuvette Types
  80
- Inserting the Cuvette
  81
- I-Control and the Cuvette Port
  82
- I-Control Cuvette Examples
  83
- Absorbance System - Infinite ® F200
  87
- Light Source System
  88
- Absorbance Optics
  88
- Absorbance Detection
  88
- Luminescence System
  89
- Luminescence Optics
  90
- Luminescence Detection
  92
6 Operating the Infinite ® 200

93
- Introduction
  93
- General Operating Features
  94
- Instrument Start up
  94
- Finish a Measurement Session
  94
- General Options
  95
- Optimize Fluorescence Measurements
  96
- Instrument Parameters
  96
- FI Ratio Mode
  98
- Measurement Accessories
  99
- FP Measurements
  102
- Fluorescence Polarization
  102
- Measurement Blank Range
  102
- G-Factor Settings
  103
- Measurement with an Uncalibrated G-Factor
  103
- Measurement with a Simultaneous G-Factor Calibration
  104
- Measurement with a Calibrated G-Factor
  105
- Measurement with a Manual G-Factor
  106
- Calculation of Fluorescence Polarization Parameters
  107
- Optimize Absorbance Measurements
  108
- Measurement Parameters
  108
- Absorbance Ratio Mode
  108
- Multiple Reads Per Well
  109
- MRW Type
  109
- MRW Size
  110
- MRW Border
  110
- Result Display in MS Excel
  112
- Miscellaneous Software Features of MRW
  112
- Optimize Luminescence Measurements
  113
- Integration Time
  113
- Light Level Attenuation
  113
7 Instrument Features

115
- Introduction
  115
- Instrument Specifications
  116
- Fluorescence Intensity and Time Resolved Fluorescence (TRF)
  117
- Definition of the Detection Limit
  118
- Fluorescein (Fluorescence Intensity) Top
  118
- Fluorescein (Fluorescence Intensity) Bottom
  118
- Europium (Time Resolved Fluorescence)
  118
- Fluorescence Polarization (FP- Infinite ® F200 Only)
  119
- Absorbance
  120
- Glow Type Luminescence
  121
- ATP Glow Luminescence
  121
- Flash Type Luminescence
  122
- Dual Color Luminescence
  123
- On the Fly" Measurements
  123
- Cuvette Measurements (Infinite ® M200 Only)
  124
- Injector
  125
8 Quality Control

127
- Periodic Quality Control Tests
  127
- Specifications - Passed/Failed Criteria
  128
- Specifications - Test Instructions
  129
- Fluorescence Top
  129
- Fluorescence Bottom
  133
- Time Resolved Fluorescence
  136
- Fluorescence Polarization (Infinite ® F200 Only)
  138
- Glow Luminescence
  140
- Absorbance Accuracy
  141
- Absorbance Wavelength Accuracy
  142
- Absorbance Baseline Flatness
  143
- Absorbance Baseline Flatness
  144
- M200 Only)
  145
- Absorbance Cuvette
  145
9 Cleaning and Maintenance

147
- Introduction
  147
- Liquid Spills
  148
- Instrument Disinfection
  149
- Disinfection Solutions
  149
- Disinfection Procedure
  150
- Disinfection Certificate
  151
- Disposal
  152
- Disposal of Packing Material
  153
- Disposal of Operating Material
  153
- Disposal of the Instrument
  154
10 Troubleshooting

155
Index

159
Tecan Customer Support

161

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