tecan Infinite 200 PRO Instructions For Use Manual
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tecan Infinite 200 PRO Instructions For Use Manual

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Instructions for Use for
Infinite 200 PRO
Document Part No.: 30052730
2016-04
Document Revision No.: 1.6

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Summary of Contents for tecan Infinite 200 PRO

  • Page 1 Instructions for Use for Infinite 200 PRO Document Part No.: 30052730 2016-04 Document Revision No.: 1.6...
  • Page 2 CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED IN THIS DOCUMENT BEFORE OPERATING THE INSTRUMENT. Notice Every effort has been made to avoid errors in text and diagrams; however, Tecan Austria GmbH assumes no responsibility for any errors, which may appear in this publication.
  • Page 3 ®, ® Infinite i-control , GCM , magellan , NanoQuant Plate ,Tecan and the Tecan Logo are registered trademarks of Tecan Group Ltd., Männedorf, Switzerland • ® ® Windows and Excel are registered trademarks of Microsoft Corporation, Redmond, WA, USA •...
  • Page 4 Manufactured by Date of manufacture Conformité Européenne Read the Instructions for Use before operating the instrument Order number Serial Number USB label WEEE symbol RoHS Orange Logo TÜV NRTL IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 5: Table Of Contents

    Transport and Storage ............... 50 3.3.1 Transport ....................50 3.3.2 Storage ....................50 Power Requirements ................51 Switching the Instrument On ............52 Gas Control Module (Enhanced) ..............53 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 6 Measurement with a Calibrated G-Factor ..........99 5.6.7 Measurement with a Manual G-Factor ..........100 5.6.8 Calculation of Fluorescence Polarization Parameters ......101 Optimizing Absorbance Measurements ......... 102 5.7.1 Measurement Parameters ..............102 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 7 5.10.1 Instrument Parameters ............... 108 5.11 Measurements with Injectors ............110 5.11.1 Priming and Washing of the Infinite 200 PRO ........110 5.11.2 Washing ..................... 114 5.11.3 Before Starting a Measurement with Injectors ........118 5.11.4 Injector Modes (i-control) ..............118 5.12...
  • Page 8 Disposal ....................182 8.4.5 Disposal of Packing Material .............. 182 8.4.6 Disposal of Operating Material ............182 8.4.7 Disposal of the Instrument ..............183 Troubleshooting .................... 185 Index ........................189 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 9: Safety

    3. Observe all WARNING and CAUTION statements in this document. 4. Never open the housing of the Infinite 200 PRO while the instrument is plugged into a power source. 5. Never force a microplate into the instrument.
  • Page 10 MATERIAL. MAKE SURE THAT ONLY QUALIFIED PERSONNEL OPERATE THE INSTRUMENT. IN CASE OF SERVICE OR WHEN RELOCATING OR DISPOSING OF THE INSTRUMENT, ALWAYS DISINFECT THE INSTRUMENT ACCORDING TO THE INSTRUCTIONS GIVEN IN THIS MANUAL. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 11: General Description

    General Description Instrument The Tecan Infinite 200 PRO is a multifunctional microplate reader with injector option. The Infinite 200 PRO provides high performance for the vast majority of today’s microplate applications and research and is robotic compatible. 2.1.1 Intended Use...

  • Page 12: Multifunctionality

    Switching between measurement techniques or plate formats is fully automated via software. It is not necessary to manually reconfigure the optics in order to switch between the reading modes supported by the Infinite 200 PRO. Both instrument versions, the filter-based Infinite F200 PRO and the monochromator-based Infinite M200 PRO, may be equipped with up to two injectors.

  • Page 13: Performance

    ‘Plate In/Out’ button, the current position of the plate carrier is automatically recognized and the plate is moved into or out of the instrument. Figure 1: Onboard of the Infinite 200 PRO. The ‘Plate In/Out’ button is located in the front right corner of the top cover.

  • Page 14: Rear View

    REMOVING OR BREAKING Warranty Label: THIS SEAL VOIDS WARRANTY!” CAUTION STOP ONLY TECAN AUTHORIZED SERVICE TECHNICIANS ARE ALLOWED TO OPEN THE INSTRUMENT. REMOVING OR BREAKING THE WARRANTY SEAL VOIDS THE WARRANTY. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 15: Software

    SBS standard 384- well plate. Figure 3: Injector-box with bottle holders 2.3.1 Injector Measurement Modes The injectors of the Infinite 200 PRO can be used with the following measurement modes: • Fluorescence Intensity top and bottom •...

  • Page 16: Injector Module Diagram

    2.3.2 Injector Module Diagram Figure 4: Schematic view of the injector module 2.3.3 Injector Pump Options There are up to two pumps available for the Infinite 200 PRO (see Figure 4 above): • Pump A feeds injector needle A •...

  • Page 17: Storage Bottles And Bottle Holders

    The tubes from the injector syringe can be inserted into a carbon needle reaching down to the bottom of the flask to ensure the optimal aspiration of even small volumes of fluid. Figure 5: Bottle holders 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 18: Injector Carrier

    CAUTION THE INJECTOR CARRIER MUST BE IN THE SERVICE POSITION STOP FOR WASHING UND PRIMING. PRIME AND WASH MUST NOT BE PERFORMED WHEN THE INJECTOR IS IN THE INSTRUMENT! IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 19 IF THE INJECTOR CARRIER IS NOT INSERTED CORRECTLY IN THE STOP INJECTOR PORT, THE INJECTOR SENSOR WILL NOT DETECT THE INSERTED INJECTOR AND THEREFORE WASHING AND PRIMING WILL BE ENABLED, WHICH CAN DAMAGE THE INSTRUMENT. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 20: Measurement Techniques

    2. General Description Measurement Techniques The following sections provide an introduction to the Infinite 200 PRO measurement techniques when fully equipped. To keep this compact, a few simplifications have been made. For details see the references. 2.4.1 Fluorescence The Infinite 200 PRO offers the basic fluorescence measurement technique and some even more sophisticated variants: •...
  • Page 21 Fluoroimmunoassays by T. Stâhlberg, et. al. - American Laboratory, December 1993 page 15]), some of them having fluorescence lifetimes in excess of 100 microseconds. The Infinite 200 PRO uses a Flash lamp light source with flash duration much shorter than fluorescence lifetime of these species. This offers the opportunity to measure fluorescence emission at some time, when stray light and prompt fluorescence have already vanished (Lag Time).

  • Page 22: Absorbance

    If the sample contains only one species absorbing in that narrow band of wavelengths, the background corrected absorbance (A) is proportional to the corresponding concentration of that species (Lambert-Beer's Law). IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 23: Luminescence

    2.4.3 Luminescence Glow Type Chemi- or Bioluminescence The Infinite 200 PRO provides measurement of glow type chemi- or bioluminescence. Glow type means that the luminescence assay glows much longer than a minute. Luminescence substrates are available, which provide stable enough light output over hours.

  • Page 24: Optical System

    Each monochromator unit, (2) and (4), is built of two gratings and a schematic view is displayed in more detail in the figures below. Fluorescence Intensity Top Diagram Figure 8: Optical System Fluorescence Top IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 25 2. General Description Fluorescence Intensity Bottom Diagram Figure 9: Optical System Fluorescence Bottom Figure 10: Detailed view of excitation and emission double monochromator unit 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 26 PRO monochromator system is < 9 nm for wavelengths > 315 nm and < 5 nm for wavelengths ≤ 315 nm. For a more detailed description of how a monochromator works, see below. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 27 The light energy of single flashes may fluctuate slightly. To take these variations into account, a silicon photodiode monitors the energy of every single flash. Fluorescence and Absorbance measurement results are compensated correspondingly. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 28 As excitation light is reflected by the sample fluid, z-adjustment helps to maximize the signal-to-noise ratio. For further details about z-positioning see chapter 5.5.2 Z-Optimization (FI Top measurements with the Infinite M200 PRO only). IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 29 PMT output current. Adjusting the PMT gain enables measurement of a wide range of concentrations in lower or higher concentration domains. For details, see Section 5.5.1 Instrument Parameters. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 30: Fluorescence Intensity System (Infinite F200 Pro)

    12. The solid arrows indicate the excitation light path; the dashed arrows determine the emission light path. Fluorescence Intensity Top Diagram Figure 11: Fluorescence intensity top system of the Infinite F200 PRO IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 31 2. General Description Fluorescence Intensity Bottom Diagram Figure 12: Fluorescence intensity bottom system of the Infinite F200 PRO 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 32 The objective lenses are made from fused silica. This material provides high UV transmission and is virtually void of auto-fluorescence. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 33 The emitted light is focused onto the excitation bottom fiber, which guides the light over a mirror through the emission filter to the fluorescence detection system. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 34: Fluorescence Polarization System (Infinite F200 Pro)

    535 nm respectively, and can be applied for measuring, for example, fluorescein- based FP applications. For details on how to mount polarizers and FP filters please refer to chapter 5.4 Defining Filter Slides (Infinite F200 PRO only). IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 35: Absorbance System (Infinite F200 Pro)

    The absorbance system is shown in Figure 13 and consists of the following components: • Light Source • Absorbance Optics • Absorbance Detection Unit Figure 13: Absorbance System of the Infinite F200 PRO 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 36 4 OD. Note For absorbance measurement of nucleic acids in small volumes (2 µl) use Tecan’s NanoQuant Plate With this device it is possible to measure 16 different samples in one measurement. For further information please contact your local Tecan distributor or visit: www.tecan.com.

  • Page 37: Absorbance System (Infinite M200 Pro)

    Figure 14: Optical System Absorbance Infinite M200 PRO For details about the light source (1) and the excitation monochromator (2), please refer to Fluorescence Intensity Light Source System, page 26. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 38 4 OD. Note For absorbance measurement of nucleic acids in small volumes (2 µl) use Tecan’s NanoQuant Plate With this device it is possible to measure 16 different samples within one measurement.

  • Page 39: Luminescence System

    2. General Description 2.5.6 Luminescence System The Infinite 200 PRO Luminescence System consists of the following parts: • Luminescence Optics • Detection Unit (Basic or Standard PMT) Figure 15: Optical System Luminescence The luminescence fiber bundle guides the light from the sample to the detection unit (PMT) passing through a filter wheel.
  • Page 40 2. General Description Luminescence Optics In luminescence measurement mode, the Infinite 200 PRO uses fixed microplate position and a moveable luminescence measurement head (see Figure 15: Optical System Luminescence). The plate thickness is defined by selecting the corresponding plate type in the software (see i-control Instructions for Use).
  • Page 41 2. General Description Figure 17: Transmission spectrum of filter ‘Lumi Green’ Wavelength [nm] Figure 18: Transmission spectrum of filter ‘Blue 1’ Wavelength [nm] Figure 19: Transmission spectrum of filter ‘Green 1’ 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 42: Cuvette Port (Infinite M200 Pro)

    The figure also shows the light path of the absorbance microplate module (5). For details of the light source (1) and the excitation monochromator (2), please refer to chapter 2.4.1 Fluorescence/A) Fluorescence Intensity (FI). IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 43 The cuvette port is an option of the Infinite M200 PRO only. This option is not available for the Infinite F200 PRO. With the Infinite F200 PRO, cuvettes may be measured using a Tecan Cuvette Adapter placed on the plate transport.
  • Page 44 The cuvette has to be inserted so that the measurement window of the cuvette corresponds to the measurement window of the cuvette holder: Figure 22: How to insert the cuvette into the cuvette holder IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 45: Alphascreen/Alphalisa System (Infinite F200 Pro Only)

    Alpha assays. The mirror is selected automatically for all Alpha measurements. Mirror type Reflection (Excitation) Transmission (Emission) 650 dichroic (AlphaScreen/AlphaLISA) 665 – 700 nm 540 – 635 nm 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 46 For fastest-possible measurement speed, the gain may be determined in a pre-measurement of the well with the highest signal and then set manually. For details see Section 5.5.1 Instrument Parameters. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 47: Installation

    15 ml bottle CAUTION STOP THE READER HAS BEEN TESTED WITH THE SUPPLIED USB CABLE. IF ANOTHER USB CABLE IS USED, TECAN AUSTRIA CANNOT GUARANTEE THE CORRECT PERFORMANCE OF THE INSTRUMENT. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 48: Unpacking Procedure

    INSTRUMENT AND THE WALL OR ANY OTHER EQUIPMENT. CAUTION STOP THE INSTRUMENT MUST BE PLACED IN A LOCATION AWAY FROM DIRECT SUNLIGHT. ILLUMINATION > 500 LUX CAN NEGATIVELY INFLUENCE LUMINESCENCE MEASUREMENTS. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 49: Removal Of The Transport Locks

    Remove the screws and pull the plate carrier out manually. Remove the screws from the transport lock. Remove the transport lock from the plate carrier. The transport locks should be saved for further transportation purposes. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 50: Transport And Storage

    SHIPPED ONLY WITH THE ORIGINAL PACKAGING AND INSTALLED TRANSPORT LOCKS. Transport and Storage 3.3.1 Transport The Infinite 200 PRO must be shipped using the original packing and installed transport locks. Before shipping the instrument, it must be thoroughly disinfected (see 8.4 Instrument Disinfection). 3.3.2 Storage Before storing the instrument the injectors must be rinsed using a wash procedure (see 5.10.2 Priming and Washing of the Infinite 200 PRO).

  • Page 51: Power Requirements

    IF THE INSTRUCTIONS GIVEN IN THIS DOCUMENT ARE NOT CORRECTLY PERFORMED, THE INSTRUMENT WILL EITHER BE DAMAGED OR THE PROCEDURE WILL NOT BE PERFORMED CORRECTLY AND THE SAFETY OF THE INSTRUMENT IS NOT GUARANTEED. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 52: Switching The Instrument On

    CAUTION STOP THE READER HAS BEEN TESTED WITH THE SUPPLIED USB CABLE. IF ANOTHER USB CABLE IS USED, TECAN AUSTRIA CANNOT GUARANTEE THE CORRECT PERFORMANCE OF THE INSTRUMENT. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 53: Gas Control Module (Enhanced)

    Precautions must be taken when working with potentially infectious material. Make sure to treat biohazardous material according to applicable safety standards and regulations as well as good laboratory practice guidelines. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 54: Gas Control Module Features

    N gas. The GCM makes the Infinite 200 PRO ideally suited for in vitro studies of eukaryotic cell lines or extends the usage of the instrument for microplate investigations using anaerobe or facultative anaerobic bacteria.

  • Page 55: Top And Rear Views Of The Gas Control Module

    O2 Mode and Dual Mode menus are unavailable and not displayed. 4.2.2 Top and Rear Views of the Gas Control Module The pictures below show the outer features of the gas control module. Front View Rear View 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 56 The control panel, which consists of a display panel and a key pad, is located on the top of the GCM enhanced. 1. Display panel 2. Power/gas flow indicator (LED) 3. Mode key 4. Plus key 5. Minus key 6. I/O key (On/Off) (Start/Stop) IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 57: Main Menu Of Modes

    (LED blinks when gas regulation is running - LED is stable gas regulation is stopped). Press the ‘Mode’ key for 1.5 seconds to return to the ‘CO2 Mode’ display (main menu). 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 58: O2 Mode

    (LED blinks when gas regulation is running - LED is stable gas regulation is stopped). Press the ‘Mode’ key for 1.5 seconds to return to the ‘O2 Mode’ display (main menu). IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 59: Dual Mode

    - LED is stable gas regulation is stopped). Press the ‘Mode’ key for 1.5 seconds to return to the ‘Dual Mode’ display (main menu). 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 60: Manual Mode

    (LED blinks when gas valve is open - LED is stable when gas valve is closed). Press the ‘Mode’ key for 1.5 seconds to return to the ‘Manual Mode’ display (main menu). IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 61: Settings Menu

    ‘Altitude’. • Device name: select a letter: A, B, C, D for device name (using the plus and minus keys), when more than one Infinite 200 PRO with GCM is connected to a PC (maximum 4 instruments with GCM). •...

  • Page 62: Installing The Gas Control Module

    Space Requirements • Place the gas control module onto the Infinite 200 PRO or on a rigid, level, and vibration-free surface near the instrument. The GCM is 18.0 cm (7.1 in) high, 25.0 cm (9.8 in) wide, 17.0 cm (6.7 in) deep and weighs 1.5 kg (3.3 lbs).

  • Page 63: Installation Procedure

    For details see chapter 4.7 CO2 and N2 Gas Cylinders. Unpack the gas control module and place it on top or near the instrument on a flat, rigid surface (see Figure 25, below). Figure 25 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 64 Use the provided long tube with quick connector and attach the tube to the regulator of the cylinder with a plastic clamp, as depicted in Figure 27, below. Figure 27 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 65 (see Figure 28, below). Figure 28 Use the provided data cable to connect the module with the Infinite 200 PRO instrument (see Figure 29, below). USB connector for data-logging with i-control software V1.10 or higher (See IFU i-control, chapter 6.2 “Prerequisites”, for driver installation).
  • Page 66 Connect the power cord of the adapter to the 12V power socket on the rear of the gas control module (see Figure 31, below). Figure 31 Switch on the GCM only after cables and tubes are connected. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 67: Operating The Gas Control Module

    Figure 32 Heating must be switched on before using the GCM: Switch on the Infinite 200 PRO. Start the Tecan i-control software and connect to the instrument. From the Instrument tab select “Heating...”, specify a “Target Temperature” within 30°C to 42°C (1), click “Set” (2) and then click “On”...
  • Page 68 ‘CO2’ and ‘N2’ on the rear of the GCM. Ensure that all gas tubes are tightly connected to gas supply (pressure reduction valve), the gas control module, and the Infinite 200 PRO Check that the pressure is set correctly and that the valves of the gas cylinder are open to allow the flow of gas: •...
  • Page 69 This will help you to recognize, for example, when the gas supply has run out (tank is empty). If power is lost – the valves of the gas control module will close automatically. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 70 GCM Enhanced, importing logged data, data displayed in status bar, data displayed in Excel output or precautions before starting a measurement) see IFU i-control software, chapter 6. Gas Control Module (GCM) Enhanced Support. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 71: Co And N Gas Cylinders (Not Supplied Accessory)

    To protect the gas cylinder from falling, a cylinder stand or table mount (with a securing chain or strap), or gas cylinder cradle can be bought from a gas cylinder company or ordered from a laboratory catalog. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 72: Troubleshooting The Gas Control Module

    The troubleshooting table below lists possible malfunctions and errors of the gas control module and provides corrective measures on how to resolve them. The operator can correct some problems or errors without having to contact Tecan Technical Support. For situations involving more complicated malfunctions or errors, the Corrective Measures column directs you to contact the local Tecan Expertline.
  • Page 73 Error message O2 sensor detects a value Check that the correct gas is used. “O2 OUT OF higher than 21%. If the problem persists, contact your local Tecan RANGE” Expertline. Error message Data cable not connected to Verify that data cable is connected to the Gas “O2 NO...

  • Page 75: Operating The Instrument

    5. Operating the Instrument Operating the Instrument Introduction The Infinite 200 PRO is operated using a personal computer based software control. i-control or Magellan software may be used as the user interface. For details see the corresponding software Instructions for Use. This short introduction is for a general understanding of instrument parameters and operation.

  • Page 76: General Operating Features

    5. Operating the Instrument General Operating Features The Infinite 200 PRO has some general behavior and options, which are independent from a particularly selected measurement technique. 5.2.1 Instrument Start Up Before the instrument is switched ON, check if the USB interface cable is connected.
  • Page 77 5. Operating the Instrument Temperature Control Some assays require an exact operating temperature. The Infinite 200 PRO can set up a specific temperature within a specific range, provide uniformity across the plate, and keep the temperature constant above ambient. The main cooling fans stop ventilation.

  • Page 78: Defining Filter Slides (Infinite F200 Pro Only)

    The spectral rejection and the bandwidth of the fluorescence filters are optimized for achieving excellent sensitivity. Contact TECAN for filters other than those supplied on the delivered filter slides. Absorbance Filters Bandpass filters, which are commonly used in microplate readers for absorbance measurements, usually have a bandwidth of 10 nm.
  • Page 79 Incorrect Correct The end of the filter with the metal lip must face away from the light source. Figure 37: Infinite F200 PRO: Filter Slide - Direction of Light 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 80: Installing A Custom Filter

    The filter will slide out of the filter slot when the filter carrier is turned over. Do not use the filter assembly tool to remove filters. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 81 5 times. CAUTION STOP TAKE CARE TO INSERT THE POLARIZERS AND THE FILTERS INTO THE FILTER SLIDE WHEN WORKING WITH FLUORESCENCE POLARIZATION. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 82 If there are unused openings remaining after the required filters have been inserted (e.g. the emission part of an absorbance filter), filter dummies should be mounted in the holes that are still open. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 83: Defining The Filters

    Overview: The overview provides the user with the current filter slide definition. Filter Slide Description: Enter the filter slide description or the filter slide description will be generated automatically. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 84 Measurement Mode: chose from the dropdown list ‘FI’ for fluorescence intensity, ‘ABS’ for absorbance, ‘FP’ for fluorescence polarization and ‘Empty’ for filter-free positions IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 85 Description: This field can be used for individual user’s remarks about the filter, e.g. filter name, application, etc. Note No special characters (blank, ?, $, %, ., /, etc.) except '_' are allowed for the filter slide description. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 86 It is recommended to manually document the last flash counter number before replacing the filter; otherwise this information will be lost. Caution STOP Do not insert filters slides if the instrument is not switched on and connected. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 87: Optimizing Fluorescence Measurements

    Infinite 200 PRO and the voltage supply is described in Equation 1. The intended use of the Infinite 200 PRO PMT is therefore specified for gain settings from 60 to 255. Gain settings below 60 are possible, but the performance of the PMT is not specified for voltage supply <...

  • Page 88: Z-Optimization (Fi Top Measurements With The Infinite M200 Pro Only)88

    When using the ‘Z-position’ function in the instrument menu, the user can determine the appropriate z-position from a graphical plot that shows the well(s) used for z-positioning. The selected value is applied for further measurements. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 89 Additionally, if the z-position of one of the labels is defined as ‘Same as’, the label will be displayed but it cannot be selected for the z-optimization: 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 90 (signal) and one filled with buffer (blank). Both wells are scanned and the resulting signal and blank curves are shown in the graph. The z-position may now be set to the maximum signal-to-blank (S/B) ratio: IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 91 Upon clicking ‘Apply’, the selected z-position will be automatically applied to the i-control script and used for the subsequent measurement. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 92 This can give rise to fluctuations of the measured values. The effect has been observed in wells of 96-well plates and larger wells. In particular, it is critical with absorbance measurements. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 93: Fi Ratio Mode

    OS switch; therefore the switch is possible within 150 ms on an Infinite M200 PRO and 250 ms on an Infinite F200 PRO. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 94: Optimal Read

    25-28 flashes in a 96-well plate (4 single spots) is performed with 7 flashes per spot, whereas a total flash number of 29 results in 8 flashes per spot. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 95 The Optimal Read feature is not available when performing well-wise measurements. The standard MRW function for Fluorescence Intensity Bottom reads is disabled when “Optimal Read” is activated and vice versa. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 96: Fp Measurements

    (see 5.6.8). In the Measurement group box, select the Blank range by clicking Change and then selecting the wells filled with the measurement (sample) blank. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 97: G-Factor Settings

    G-Factor field. For the G-Factor calibration, see 5.6.5. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 98: Measurement With A Simultaneous G-Factor Calibration

    Note By filling in more than one well with polarization references and reference blanks, the mean values will be calculated and therefore the calibration result will be more accurate. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 99: Measurement With A Calibrated G-Factor

    A calibrated G-factor will be displayed automatically or can be loaded by clicking the '>>' button only if it matches the selected fluorescence polarization filter pair and the filter slide description. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 100: Measurement With A Manual G-Factor

    The calibrated G-Factor can be restored by clicking the ‘>>’ button on the left side of the displayed G-Factor. Note G-Factor adjustment via '>>' button is only possible, if a calibrated G-Factor is available for the corresponding wavelength. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 101: Calculation Of Fluorescence Polarization Parameters

    − cross  Intensities: Parallel and perpendicular intensities are calculated using the following formulas: ∆ ∆ cross cross Polarization: − cross cross Anisotropy: − cross cross Total Intensity: cross 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 102: Optimizing Absorbance Measurements

    The Infinite F200 PRO can switch between two neighboring filters within 250 ms. The Infinite M200 PRO can switch between two wavelengths in 150 ms. For conditions, see 5.5.3 FI Ratio Mode. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 103: Multiple Reads Per Well

    The MRW types define the pattern how the measurement will be performed. The software allows seven different MRW types to be selected: • Square • Square (filled) • Circle • Circle (filled) • X-line • Y-line • XY-line 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 104: Mrw Size

    The border is calculated from the theoretical beam diameter of the instrument. However, when measuring liquid samples, the light beam diameter is influenced by the type and amount of liquid in a well. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 105 AND THE WELL WALL OF THE MICROPLATE. CAUTION A ‘BORDER’ VALUE THAT IS TOO SMALL MAY CAUSE STOP WRONG MEASUREMENT RESULTS DUE TO OVERLAP BETWEEN THE LIGHT BEAM AND THE WELL WALL OF THE MICROPLATE. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 106: Result Display In Ms Excel

    Intensity Top’ and ‘Fluorescence Intensity Bottom’. The MRW feature is not active when performing well-wise measurements. ‘Reference Wavelength’ (located on the absorbance strip) is not available in combination with ‘Multiple Reads per Well’. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 107: Optimizing Luminescence Measurements

    Therefore, values >10,000,000 counts per second (without attenuation) are displayed as “INVALID” in the results sheet. The optical system of the Infinite 200 PRO can attenuate light levels by a fixed factor of either 1 (none) or 100 (2 OD). Correspondingly, the usable measurement range will be shifted to higher light levels (<1,000,000,000 counts...

  • Page 108: Optimizing Alphascreen/Alphalisa Measurements

    5.10.1 Instrument Parameters Gain Settings The Infinite 200 PRO AlphaScreen/AlphaLISA detection system uses an analog to digital (ADC: Analog Digital Converter) conversion of PMT signal. The gain setting controls the amplification of the PMT when converting light into electrical current.
  • Page 109 Infinite 200 PRO and the voltage supply is described in Equation 1. The intended use of the Infinite 200 PRO PMT is therefore specified for gain settings from 60 to 255. Gain settings below 60 are possible, but the performance of the PMT is not specified for voltage supply <...

  • Page 110: Measurements With Injectors

    5. Operating the Instrument 5.11 Measurements with Injectors 5.11.1 Priming and Washing of the Infinite 200 PRO CAUTION THE INJECTOR CARRIER MUST BE IN THE SERVICE POSITION STOP FOR WASHING UND PRIMING. PRIME AND WASH MUST NOT BE PERFORMED WHEN THE INJECTOR IS IN THE INSTRUMENT! The initial filling step of the injector system (priming) as well as the cleaning step of the injector system (washing) must take place outside of the instrument.
  • Page 111 (A or B) on the injector box. When using the hardware buttons for priming, these settings will be applied. 10. Select ‘Close’ to exit the dialog box 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 112 Remove the injector carrier from the instrument and insert the injector carrier into the service position of the injector box. Insert the feeding tubing into the appropriate storage bottle. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 113 16. Click Close to exit the dialog box. CAUTION THE INJECTOR CARRIER MUST BE IN THE SERVICE POSITION STOP FOR THE ACTION ‘BACKFLUSH’. DO NOT PERFORM BACKFLUSH WHEN THE INJECTOR IS IN THE INSTRUMENT! 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 114: 5.11.2 Washing

    Select the Refill Speed (100 – 300 µl/sec.) or select Refill Speed equal to Wash Speed. Click Start wash to start the wash procedure. Click Close to exit the dialog box. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 115 PRESS THE BUTTON FOR LESS THAN 3 SECONDS TO STOP START PRIME. • PRESS THE BUTTON FOR MORE THAN 3 SECONDS TO START WASH. THE PARAMETERS HAVE TO BE SET IN THE i-control SOFTWARE. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 116 The maximum filling volume is 1.5 ml. An internal counter checks the dispensed liquid volumes; the software alerts the user when it is time to empty the waste tub. Figure 44: Waste tub on plate carrier IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 117 TREAT THE USED MICROPLATE, OTHER DISPOSABLES, AND ALL SUBSTANCES USED, IN ACCORDANCE WITH GOOD LABORATORY PRACTICE GUIDELINES. INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY, STATE OR REGION. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 118: 5.11.3 Before Starting A Measurement With Injectors

    • The tubes are clean. If not please refer to chapter 5.10.2 Priming and Washing of the Infinite 200 PRO for details how to clean the injector system. • The injector tubes are correctly inserted into the storage bottles and fixed.
  • Page 119 The maximum dispense volume is 800 µl/dispense strip. If volumes greater than 800 µl are to be dispensed (e.g. into 6-well plates), more than one dispense strip has to be used. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 120 The maximum injection volume is 800 µl/injection strip. If volumes greater than 800 µl are to be injected (e.g. into 6-well plates), more than one injection strip has to be used. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 121 It is mandatory to use at least one measurement strip in combination with the injection strip. The position of the measurement strip(s) (before and/or (Example after the injection strip) depends on the application and is therefore user- Absorbance) selectable. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 122 If ‘Wait for injection’ is selected, the wait time includes the injection time. If ‘Wait for injection’ is NOT selected, the wait time is added to the injection time. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 123: Blanking Measurements

    BLANKING DATA WILL BE OVERWRITTEN WITHOUT A WARNING STOP MESSAGE WHEN STARTING ANOTHER BLANKING MEASUREMENT. BLANKING DATA WILL BE DELETED WITHOUT A WARNING i-control MESSAGE WHEN CLOSING THE SOFTWARE. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 124: Cuvette Measurements

    The cuvette can be moved in and out with the ‘cuvette in’ and ‘cuvette out’ buttons or by selecting Cuvette in/Cuvette out in the Instrument/Movements dialog box. Figure 46: Cuvette ‘out’ and ‘in’ button IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 125: 5.13.3 I-Control Cuvette Examples

    Example of how to use the ‘Blanking’ measurement when measuring a DNA sample: Prepare cuvette with sample buffer Set up the DNA measurement in the i-control software: Select Blanking from the Instrument menu: 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 126 After finishing the measurement of the first cuvette (Cuv: 1) the following message is displayed: Click No to finish the measurement. Click Yes to continue: Insert the next sample cuvette and click OK to continue the measurement. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 127 Reference wavelength is only allowed on the first absorbance strip. Absorbance strip Reference wavelength is disabled on absorbance strips 2 to 10. (microplate) Select the appropriate measurement parameters (wavelength, number of flashes and settle time) for your application. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 128 Move Plate Optional. To move the microplate automatically out of the instrument when finishing the measurement, select the ‘Move plate OUT’. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 129: I-Control Examples

    Plate is moved out after finishing all wells CAUTION SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE STOP STARTING A LUMINESCENCE MEASUREMENT. SOME COMPONENTS NEED TO WARM UP TO GUARANTEE STABLE CONDITIONS FOR THE MEASUREMENT. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 130 Plate is moved out after finishing all wells CAUTION SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE STOP STARTING A LUMINESCENCE MEASUREMENT. SOME COMPONENTS NEED TO WARM UP TO GUARANTEE STABLE CONDITIONS FOR THE MEASUREMENT. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 131 Select the appropriate parameters for the second label: Excitation intensity (2) wavelength: 340 nm, Emission wavelength: 510 nm; number of flashes: 25; integration time: 40; gain: manual Move Plate Plate is moved out after finishing all wells 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 132 Select the appropriate parameters for the second label: Excitation intensity (2) wavelength: 340 nm, Emission wavelength: 480 nm; number of flashes: 25; integration time: 40; gain: manual Move Plate Plate is moved out after finishing all wells IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 133: Finishing A Measurement Session

    Upon shut down, the instrument activity is stopped immediately. Normally, you should disconnect before shut down. In the rare case of an unexpected hardware error, immediate instrument shut down will reduce the risk of possible damage. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 135: Instrument Features

    ANSI/SBS 1-2004; ANSI/SBS 2-2004; ANSI/SBS 3-2004 and ANSI/SBS 4-2004. The instrument can perform kinetic measurements. Reading may be restricted to one part of the microplate. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 136: Instrument Specifications

    9.84 inches Pump Module Height: 155 mm 6.10 inches Depth: 156 mm 6.14 inches Weight: Infinite F200 PRO 14.0 kg Infinite M200 PRO 15.8 kg Pump Module 3.4 kg IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 137 + 140 °F) Relative Humidity: Operation < 80 % non condensing Over-voltage Category Usage General Laboratory Instrument Noise Level < 60 dBA Pollution Degree Electronic waste Method of Disposal (infectious waste) 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 138: Fluorescence Intensity And Time Resolved Fluorescence (Trf)

    Optimal automatic 0 - 60,000 RFU Calculated from automatic 0 - 60,000 RFU Well TRF Parameters Characteristics Integration Time 10 - 2000 µs Lag Time 0 - 2000 µs IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 139: Definition Of The Detection Limit

    Plate Type (number of wells) Dispensed Volume [µl] Flashes (Reads) per Well Europium Detection Limit < 150 fM (Infinite F200 PRO) Europium Detection Limit < 5 pM (Typical value) (Infinite M200 PRO) 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 140: Fluorescence Polarization (Fp)

    0 - 60,000 RFU FP Parameters Characteristics Integration Time 20 - 2000 µs Lag Time 0 - 2000 µs FP Precision (F200 only) < 5 mP @ 1nM Fluorescein IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 141: Absorbance

    0 – 4 OD Range The following specifications are valid for the wavelength range from 300 – 700 nm for Infinite 200 PRO series. Plate type (number of wells) < ± (1 % + 10 mOD) Accuracy 0 – 2 OD <...

  • Page 142: Glow Type Luminescence

    Total Dispensed Volume[µl] Integration Time/well [ms] 1000 ATP Detection Limit 3 fmol/well CAUTION STOP SPECIFICATIONS ARE ONLY VALID WHEN THE INSTRUMENT IS PLACED IN A LOCATION WITH ILLUMINATION < 500 LUX. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 143: Flash Type Luminescence

    Attenuation of Light 1 (no attenuation) ATP Detection Limit < 80 amol/well CAUTION STOP SPECIFICATIONS ARE ONLY VALID WHEN THE INSTRUMENT IS PLACED IN A LOCATION WITH ILLUMINATION < 500 LUX. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 144: Dual Color Luminescence (E.g. Bret)

    AlphaScreen/AlphaLISA CAUTION SWITCH ON THE INSTRUMENT AT LEAST 15 MINUTES BEFORE STOP STARTING AN ALPHASCREEN/ALPHALISA MEASUREMENT. SOME COMPONENTS NEED TO WARM UP TO GUARANTEE STABLE CONDITIONS FOR THE MEASUREMENT. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 145: On The Fly" Measurements

    6.10 “On the Fly” Measurements “On the Fly” measurements are the fastest measurements possible using the Infinite 200 PRO. These measurements are performed using only one flash (number of flashes). 96-well plates (FI, TRF, Absorbance) Measurement time: < 20 s 384-well plates (FI, TRF, Absorbance) Measurement time: <...

  • Page 146: Cuvette Features (Infinite M200 Pro Only)

    CAUTION STOP THE CUVETTE PORT OF INFINITE M200 PRO CANNOT BE USED FOR CUVETTES WITH A MEASUREMENT WINDOW < 2 MM (DIAMETER) AND A CENTER HEIGHT BELOW 15 MM. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 147: Injector Specifications

    Water, Fresh Sodium Hypochlorite Concentrated Sulfuric Acid CAUTION THE INFORMATION IN THIS TABLE HAS BEEN SUPPLIED TO TECAN AUSTRIA BY OTHER REPUTABLE SOURCES AND IS TO BE USED STOP ONLY AS A GUIDE IN SELECTING EQUIPMENT FOR APPROPRIATE CHEMICAL COMPATIBILITY. BEFORE PERMANENT INSTALLATION, TEST THE EQUIPMENT WITH THE CHEMICALS AND UNDER THE SPECIFIC CONDITIONS OF YOUR APPLICATION.

  • Page 148: Measurement Accessories

    6.13 Measurement Accessories 6.13.1 Recommended Filters (Infinite F200 PRO only) Please ask your local Tecan dealer for a recommended filter set. Filters designed for a different type of instrument will not necessarily perform well with the Infinite F200 PRO. Note...

  • Page 149: 6.13.2 Recommended Types Of Microplates

    GRE384fb 781 079 781 076 781 086 781 094 781 077 781 095 GRE384ft 781 061 781 186 AC-0205 781 101 781 165 781 162 781 182 781 185 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 150 DWG00120 COS96fw 3362 3922 3912 COS96rt 3360 3795 DWG01123 3367 3358 3788 COS96ft_half area 3690 3697 DWG00122 3695 COS384fb 3708 3710 DWG00679 3709 COS384ft 3680 3701 DWG00679 3700 3702 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 151 237 105 437 112 237 107 NUN96fw_LumiNunc FluoroNunc 136 101 236 108 MTP-0004 136 102 436 110 236 105 436 111 236 107 BD Falcon BD24_FluoroBlok 351155 351157 MTP-0005 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 152 6008350 TechnicalDataS heet_Dimension PE384fg_ProxiPlate 6008270 sOfProxiplate- 384Plus PE384fw_ProxiPlate 6008280 PE384fw_OptiPlate 6008290 TechnicalDrawin g2: Dimensions apply to 384 well OptiPlates Table 1: Plate definition files and the corresponding catalog numbers IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 153: 6.13.3 Luminescence Detection

    NEED TO WARM UP TO GUARANTEE STABLE CONDITIONS FOR THE MEASUREMENT. The Infinite 200 PRO luminescence detection system utilizes the single photon counting measurement technique. This is based on a dedicated luminescence PMT with appropriate measurement circuitry. This technique is very robust against noise.

  • Page 155: Quality Control

    STOP THE SPECIFICATIONS OF THE INSTRUMENT. IF THE RESULTS OF THESE CONTROL TESTS DO NOT LIE WITHIN THE OFFICIAL SPECIFICATIONS OF THE INSTRUMENT, PLEASE CONTACT YOUR LOCAL SERVICE CENTER. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 156: Specifications - Passed/Failed Criteria

    Specifications - Passed/Failed Criteria Note All specifications are subject to change without prior notification. The following table gives an overview of the passed/failed criteria for the specification test of the Infinite 200 PRO. Specification Passed/Failed Criteria Fluorescence Top Sensitivity < 20 pM Fluorescein Fluorescence Top Uniformity <...

  • Page 157: Specifications - Test Instructions

    7. Quality Control Specifications - Test Instructions 7.3.1 Fluorescence Top For the Infinite 200 PRO with the option ‘Fluorescence Top’, the following tests can be performed to prove the specifications: • Sensitivity • Uniformity • Precision These test instructions are valid for the Infinite 200 PRO: •...
  • Page 158 Average RFU value of wells filled with blank stdev Standard deviation of RFU values of wells filled with blank The result of the formula 'Detection Limit' determines the sensitivity in pM units. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 159 Average RFU value of wells filled with fluorophore stdev Standard deviation of RFU values of wells filled with fluorophore The result of the formula determines the uniformity in % CV. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 160 Average RFU value of well A1 over the 20 kinetic wellA1 stdev Standard deviation of RFU values of Well A1 over the 20 cycles wellA1 The result of the formula determines the Precision in % CV. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 161: Fluorescence Bottom

    7. Quality Control 7.3.2 Fluorescence Bottom For the Infinite 200 PRO with the option ‘Fluorescence Bottom’ the following tests may be performed to prove the specifications: • Sensitivity (Standard) • Sensitivity (Enhanced) • Uniformity • Precision/Repeatability These test instructions are valid for the Infinite 200 PRO: •...
  • Page 162 Plate Type GRE96fb Plate Layout Pipette 200 µl of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the appropriate wells according to the plate layout: <> IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 163 0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045) 1 Greiner 96-well plate µClear, black with transparent bottom 200 µl Pipette + tips Calculation of Uniformity: See 7.3.1 Fluorescence Top: Uniformity 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 164 0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045) 1 Greiner 96-well plate µClear, black with transparent bottom 200 µl Pipette + tips Calculation of Precision: See 7.3.1 Fluorescence Top: Precision. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 165: Time Resolved Fluorescence

    7. Quality Control 7.3.3 Time Resolved Fluorescence For the Infinite 200 PRO with the option ‘Fluorescence Top’, the following tests may be performed to prove the specifications: • Sensitivity • Precision/Repeatability These test instructions are valid for the Infinite F200 PRO.
  • Page 166 Plate Type GRE96fw Part of the plate Kinetic 20 Cycles Interval Time Minimal Plate Layout: See 7.3.3 Time Resolved Fluorescence Precision. Calculation of Precision: See 7.3.1 Fluorescence Top: Precision. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 167: Fluorescence Polarization (Infinite F200 Pro Only)

    Time between Move and Read Gain Optimal Plate Type GRE96fb Reference from/to A1 – D1 Reference blank from/to A2 – D2 Reference value 20 mP Measurement blank from/to same as reference blank 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 168 Calculation of Precision: The precision is calculated from the wells filled with fluorescein. The precision corresponds to one time standard deviation of the mP values of the fluorescein wells. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 169: Glow Luminescence

    7. Quality Control 7.3.5 Glow Luminescence For the Infinite 200 PRO with the option ‘Luminescence the following tests may be performed to prove the sensitivity specifications: Sensitivity Perform the following measurement to determine the sensitivity: Measurement Parameters: Parameter Setting Reading Mode...

  • Page 170: Absorbance Accuracy

    Material/Reagents: MultiCheck plate Calculation of Wavelength Accuracy: Please refer to the data sheet in the instructions for use for your MultiCheck plate. − Wavelength Accuracy theoretical maximum measured maximum IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 171: Absorbance Baseline Flatness (Infinite M200 Pro)

    No plate is necessary for measurement – the plate carrier has to be empty for this measurement. Material/Reagents: No material or reagents necessary for this test. Calculation of Baseline Flatness: Calculate the standard deviation. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 172: Absorbance Baseline Flatness (Infinite F200 Pro)

    No plate is necessary for measurement – the plate carrier has to be empty for this measurement. Material/Reagents: No material or reagents necessary for this test. Calculation of Baseline Flatness: Calculate the standard deviation over 20 cycles for each wavelength. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 173: Absorbance Cuvette (Infinite M200 Pro Only)

    RM-N1N35N + D3 cuvette (for more information please refer to www.starna.co.uk) Calculation of Accuracy: Calculate the deviation of the measured value from the reference value supplied with the calibrated cuvette. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 174: Alphascreen/Alphalisa (Infinite F200 Pro Only)

    10,000 ng/ml 5,000 ng/ml 2,500 ng/ml 1,250 ng/ml 625 ng/ml 312 ng/ml 156 ng/ml 78 ng/ml 39 ng/ml 20 ng/ml 10 ng/ml 5 ng/ml 2.5 ng/ml Blank (PBS only) IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 175 0 and solve the trend line equation (y = kx) using the 3-fold standard deviation of the blank as y. 3* stdev of the blank (wells N2-P6) Extrapolate the detection limit [ng/ml] by using the 3-fold standard deviation of the blank as y. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 177: Cleaning And Maintenance

    PRECAUTIONS, (INCLUDING THE WEARING OF POWDER-FREE GLOVES, SAFETY GLASSES AND PROTECTIVE CLOTHING) TO AVOID POTENTIAL INFECTIOUS DISEASE CONTAMINATION WHEN PERFORMING CLEANING PROCEDURES AND ALSO WHEN MAKING ADJUSTMENTS TO THE INSTRUMENT. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 178: Liquid Spills

    CAUTION STOP TO AVOID REAGENT MIXING AND CROSS-CONTAMINATION, WASH THE WHOLE INJECTOR SYSTEM THOROUGHLY BETWEEN DIFFERENT APPLICATIONS USING THE INJECTOR. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 179: Daily Maintenance

    Clean the end of the injector needles with a cotton swab soaked in 70 % ethanol or isopropanol. WARNING RISK OF FIRE AND EXPLOSION! ETHANOL IS FLAMMABLE AND WHEN IMPROPERLY HANDLED CAN LEAD TO EXPLOSIONS. PROPER LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED. 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...

  • Page 180: Instrument Disinfection

    8.4.1 Disinfection Solutions The instrument should be disinfected using the following solution: • B30 (Orochemie, Max-Planck-Str. 27; D-70806 Kornwestheim) IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 181: Disinfection Procedure

    If a Safety Certificate is not supplied, the instrument may not be accepted by the service center. Your local Tecan customer support can send you a new copy of the Safety Certificate, if required. 2016-04 IFU for Infinite 200 PRO No.

  • Page 182: Disposal

    TREAT THE USED MICROPLATE, OTHER DISPOSABLES, AND ALL SUBSTANCES USED, IN ACCORDANCE WITH GOOD LABORATORY PRACTICE GUIDELINES. INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND APPROVED METHODS OF DISPOSAL IN YOUR COUNTRY, STATE OR REGION. IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...

  • Page 183: Disposal Of The Instrument

    8. Cleaning and Maintenance 8.4.7 Disposal of the Instrument Please contact your local Tecan service representative before disposing of the instrument. CAUTION STOP ALWAYS DISINFECT THE INSTRUMENT BEFORE DISPOSAL. Pollution degree 2 (IEC/EN 61010-1) Method of disposal Contaminated waste WARNING...

  • Page 185: Troubleshooting

    Tecan customer support office. X drive init error Hardware Failure Plate Transport Module. Please report this error to your local Tecan customer support office. Y drive init error Hardware Failure Plate Transport Module. Please report this error to your local Tecan customer support office.
  • Page 186 Tecan customer support office. Invalid Command: [cmd] Unspecific internal communication error. Please report this error to your local Tecan customer support office. Invalid Operand: [cmd] Unspecific internal communication error. Please report this error to your local Tecan customer support office.
  • Page 187 Wavelength:[lambda] local Tecan customer support office. Gain:[g], Counts: [cts] Steploss Error Actuator failure. Please report this error to your local Tecan customer support office. Sync Scan: Number of EX- Unspecific error in the Instrument - Computer Steps does not match EM- communication protocol.

  • Page 189: Index

    Emission Filter ........... 34, 46 Luminescence ..........23 Excitation ............32 Luminescence Detection Unit ......39 Excitation Spot Size ......... 28 Luminescence Optics ........39 Luminescence System ........39 2016-04 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6...
  • Page 190 Bottom Fluorescence ......... 164 voltage range ........... 51 Fluorescence Polarization ......167 Time resolved fluorescence ....... 166 Top Fluorescence ........160 Wavelength Accuracy ........170 Wavelength Switch Time ......... 93 IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6 2016-04...
  • Page 191 Tecan Customer Support Tecan Customer Support If you have any questions or need technical support for your Tecan product, contact your local Tecan Customer Support organization. Go to http://www.tecan.com/ for contact information. Prior to contacting Tecan for product support, prepare the following information...
  • Page 192 TECAN AUSTRIA GMBH, Untersbergstrasse 1a, A-5082 Grödig / Salzburg, Austria T +43 62 46 89 33, F +43 62 46 72 770, office.austria@tecan.com, www.tecan.com Declaration of Conformity We, TECAN Austria GmbH herewith declare under our sole responsibility that the product identified as:...

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