Absorbance; Glow Type Luminescence - tecan GENios Operating Manual

5. Instrument Features
5.1.2

Absorbance

5.1.3

Glow Type Luminescence

5-4 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1
The specifications only apply to 96 well plates.
Absorbance can not be measured in 1536 well plates.
Parameters
Wavelength
range
Meas. range
Resolution
Accuracy
Precision
Linearity
No. of Flashes /
Well
Standard filters
The specifications only apply to 96 well plates.
Luminescence can only be measured in 96 well plates.
Parameters
Sensitivity
Wavelength
range
Gain
Integration setting
Manual
Maximum Dynamic
Range
Characteristics
230 - 1000 nm
0 - 3 OD
0.0001 OD
0.0 – 2.0 OD 1 % and
±
2.0 - 3.0 OD ± 1.5 % and
0 - 3.0 OD ± 1 % and
0 - 3.0 OD ± 1.5 % and
1 - 255
260 nm / 280 nm / 340 nm / 595 nm
Characteristics
< 0.4 10-6 DEA activity units/well of molecular biology
grade alkaline phosphatase (20 µl/well) using
CDP-Star (Tropix) chemiluminescent substrate mix with
Emerald-II enhancer (100 µl/well) in a Greiner 96 well
white plate. Use of the provide cover plate greatly
reduces the cross-talk.
0.4 10-6 DEA activity units/well correspond to 200
fg/well of alkaline phosphatase when using a lot of 2000
DEA activity units/mg protein.
400 - 700 nm
Optimal:
Manual:
Integration time
1 - 5000 ms
automatic
(1 - 1000 ms)
0.010 OD 492 nm
±
± 0.010 OD 492 nm
± 0.005 OD 492 nm
± 0.005 OD 492 nm
preset value
1 - 255
Measurement range
0 - 55,000 RLU
0 - 55,000,000 RLU
(using XF )
LUOR
June 2002