tecan Infinite 200 PRO Instructions For Use Manual page 164

7. Quality Control
Precision
Measurement Parameters:
Plate Layout:
<> 1 2
A
B
C
D
E
F
G
H
Material/Reagents:
Calculation of Precision:
164
Perform the following measurement to determine the precision/reproducibility:
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
Part of the Plate
Kinetic
Interval Time
3 4 5
Filling volume: 200 µl
25 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
See 7.3.1 Fluorescence Top: Precision.
IFU for Infinite 200 PRO
Setting
Fluorescence Bottom
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
25
40
0
Optimal
GRE96fb
A1
20 Cycles
Minimal
6 7 8
No. 30052730 Rev. No. 1.6
9 10 11
2016-04
12