tecan Infinite 200 PRO Instructions For Use Manual page 162

7. Quality Control
Material/Reagents:
Calculation of Detection Limit (Sensitivity):
Sensitivity (Enhanced)
Measurement Parameters:
Plate Layout
<> 1 2
A
B
C
D
E
F
G
H
162
25 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl Pipette + tips
See 7.3.1 Fluorescence Top: Sensitivity.
Perform the following measurement to determine the detection limit for
Fluorescein:
Parameter
Reading Mode
Ex Wavelength
Em Wavelength
Number of flashes
Integration Time
Settle Time
Gain
Plate Type
Pipette 200 µl of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
3 4 5
IFU for Infinite 200 PRO
Setting
Fluorescence Bottom
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
25
40
0
Optimal
GRE96fb
6 7 8
No. 30052730 Rev. No. 1.6
9 10 11
2016-04
12