tecan M1000 Operating Manual Addendum
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tecan M1000 Operating Manual Addendum

For the advanced operator
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m1000™ Operating Manual Addendum
for the Advanced Operator
m1000™ Operating Manual Addendum, 392813, en, V1.1

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  • Page 1 Operating Manual Addendum for the Advanced Operator m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 2 Tecan Schweiz AG Seestrasse 103 CH-8708 Männedorf Switzerland Distributor Address Abbott Laboratories Abbott Park, IL 60064 Document Status Sheet Title: m1000™ Operating Manual Addendum for the Advanced Operator 392813 Version Revision Issue Major changes may 2003 First issue LiHa/RoMa Calibration Procedure updates New Procedure: Move Plate Test.

  • Page 3: Table Of Contents

    Calibrating the DiTi rack shelves for the RoMa .........22 Fine-tuning the DiTi rack carriers for the LiHa ..........23 Move Plate Test ....................25 Disconnect from the Instrument ..............25 GENESIS Setup & Service Procedures ............26 NAT MBS Worktable ..................27 m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 4 Table of Contents Initialise Instrument ..................29 Dilutor Tool ....................30 Door Lock Check ..................32 FaWa Test ......................36 Liquid Detection Test ...................40 Lower DiTi Eject Test ...................43 PosID Test .....................46 m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 5: M1000™ Operating Manual Addendum

    Lower DiTi Eject Test PosID Test User Qualification The laboratory personnel must be qualified and trained to operate the m1000™. The operators must fulfill the following qualifications to perform the procedures described in this Addendum: They must have a thorough knowledge of the applications run on the system.

  • Page 6: Notices And Symbols

    WARNING Automatically moving parts. Injuries (crushing, piercing) possible, if the safety panels are not in place. Before starting the m1000™, make sure that the safety door is closed. Never operate the instrument without panels on and door closed. WARNING Laser Hazard Identifies a Class II laser product that contains an embedded low power visible Class II laser diode barcode scanner.
  • Page 7 ATTENTION Symbols ATTENTIONS appear as follows: ATTENTION With the general “Read This!” symbol, ATTENTION indicates the possibility of equipment damage, malfunctions, or incorrect process results if instructions are not followed. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 8: Nat Admin Software

    NAT Admin Software NAT Admin The NAT Admin software allows you to configure the m1000™ with information to meet your site’s specific requirements. Software WARNING The Abbott 1 ml Protocol v1.0 cannot be edited. The use of the Abbott sample preparation kit and the provided Generic 1 ml Protocol will allow an operator successful RNA separation.

  • Page 9: Start Nat Admin

    The NAT Wizard must be closed to start the NAT Admin. Admin Select the NAT Admin icon. The NAT Admin Login screen is displayed. Enter User Name and Password (case sensitive) and select <OK>. The NAT Admin main screen is displayed. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 10: Nat Worktable

    NAT Worktable The following figure shows the m1000™ worktable locations used in NAT Admin software: Note: Worktable locations are fixed and cannot be changed. Fig. 1 NAT Admin Worktable Control Loc 1 ml Subsystem Loc RT Input Loc 1 ml Subsystem Loc Mag Low...

  • Page 11: Nat Options

    Database Database Maintenance is performed daily at 01:00 a.m. in the form of a daily backup. In the case of a database corruption, a previous database can be Maintenance restored. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 12: Restore Database

    The NAT Admin main screen is displayed. Select Tools from the NAT Admin main menu. Select Database Maintenance. Select the file to restore. Select Restore Now. Select Close to exit to the NAT Admin main menu. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 13: Temperature Check

    Temperature The temperature check for the 1 ml subsystem should be performed according to your laboratory procedures or as directed for troubleshooting. Refer to the Check m1000™ Operating Manual, Chapter 8, Troubleshooting, for additional assistance. WARNING Potentially infectious Instrument parts may be contaminated with potentially infectious materials.

  • Page 14: Nat Teach Worktable Racks And Locations

    Teach Worktable Racks and Locations screen. In addition, the vertical and horizontal position for the LiHa and RoMa for carriers and racks on the m1000™ worktable must be determined by using the NAT Admin Teach Worktable Racks and Locations procedures.

  • Page 15: Calibrate The Liha

    Calibrating the 1 ml subsystem for the LiHa LiHa During this step, the LiHa is calibrated to correctly aspirate and dispense specimens, controls, and reagents into the reaction vessels. This calibration will include X, Y, and Z direction. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 16 Under Calibrate LiHa, select Mount Reference Tip. The DiTi channels will separate, allowing easy access to install the reference tip to the third DiTi channel. The operator is prompted to mount the reference tip to DiTi #3. Fig. 3 Mounting the Reference Tip m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 17 1 ml Heater Zone 1 Calibration Point – 1 ml Heater Zone 2 Calibration Point – 1 ml Mag Calibration Point – 1 ml RT Calibration Point – 1 ml Mag Rest Calibration Point m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 18 Travel with the LiHa to Location on Carrier. The LiHa returns to the new position. If the position is incorrect, repeat steps 8 through 11. If the position is correct, select Travel Home. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 19: Calibrating The Diti Rack Carriers For The Liha

    During this step, the LiHa is calibrated to correctly pick up disposable tips (DiTis) used for aspiration and dispense and delivery of DiTis to the DiTi Reuse rack. This DiTi rack calibration will include X, Y, and Z direction. carriers for the LiHa m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 20 Select Travel With the LiHa to Location on Carrier. The LiHa will move to the selected position. Select a Travel increment of 1, 10, or 100, with 1 being the smallest travel increment and 100 being the largest travel increment. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 21: Removing The Diti Reference Tip

    Once all positions have been calibrated, select Dismount Reference Tip. The operator is prompted to Remove the Reference Tip. Select <OK>. Removing the DiTi Reference A. Tubing extension B. Separator ring (white) C. O-ring (black) m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 22: Calibrate The Roma

    1 ml Subsystem Loc RT – 1 ml Subsystem Loc Rest Note: 1 ml Subsystem Loc Mag Med and 1 ml Subsystem Loc Mag High will be automatically calibrated. No calibration is necessary. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 23 Travel with the RoMa to the Location. The RoMa returns to the new position. If the position is incorrect, repeat steps 5 through 6. If the position is correct, select Travel Home. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 24: Calibrating The Diti Rack Carriers For The Roma

    100 being the largest travel increment. The RoMa gripper arms should be centered on both sides of the DiTi rack and approximately 1 mm above the surface of the DiTi Rack carrier. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 25 Once the X, Y, and Z direction are saved for one DiTi rack carrier position, then repeat the steps 1 through 6 for each remaining DiTi rack carrier position to be calibrated. Fig. 11 Calibrate RoMa m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 26: Calibrating The Diti Rack Shelves For The Roma

    If the position is incorrect, repeat steps 2 through 5. If the position is correct, select Travel Home. Once the X, Y, and Z direction are saved for one Shelf location, then repeat the steps 1 through 6 for each remaining Shelf location to be calibrated. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 27: Fine-Tuning The Diti Rack Carriers For The Liha

    10 At the Sample screen, add a sample rack with eight barcode labeled tubes to grid 4, 5, or 6. 11 Select <Scan Rack> and > to continue. 12 At the Reagent Report screen, select > to continue. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 28 Note: Only adjust by 5, 10, 15, or 20 steps. DO NOT adjust the Z direction. Once each location is adjusted, select Save LiHa Calibration. 34 Raise the Z direction for only Plate Carrier 1, Loc 3 (DiTi Reuse Rack) 28 steps. Select Save LiHa Calibration. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 29: Move Plate Test

    23 If any of the positions of the DiTi rack are not correct or DiTi racks are tilted, repeat Calibrating DiTi rack shelves for the RoMa. Disconnect Once the LiHa and RoMa have been calibrated for the m1000™ worktable, return the carriers and racks, including the Waste Station Chute to the m1000™ from the worktable.

  • Page 30: Genesis Setup & Service Procedures

    View → Tool Bar Worktable (empty) Status bar Can be switched on/off with View → Status Bar The GENESIS Setup & Service main screen is divided into four sections: Menu bar Tool bar Worktable Status bar m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 31: Nat Mbs Worktable

    From the GENESIS Setup & Service screen, select System from the menu bar. Select Load Worktable from the System menu. The Select Worktable screen is displayed. Select NAT MBS Worktable and then <OK>. NAT MBS Worktable m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 32 1 ml subsystem Heater zone 1 Room temperature zone Heater zone 2 Magnet zone Waste Station Waste, RR Waste, RM DiTi Waste Reagent Trough Carrier DiTi 21-26 DiTi Rack Carriers Balance 28-37 Balance (not used) m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 33: Initialise Instrument

    Initialise During initialization of the m1000™, all installed subassemblies are moved to their initial positions. Instrument The PosID and LiHa will move to the left side. The RoMa will move to the right side. Close the Safety door. Start the GENESIS Setup & Service software by clicking on the TECAN icon.

  • Page 34: Dilutor Tool

    Start the GENESIS Setup & Service software by clicking on the TECAN icon. From the GENESIS Setup & Service main screen, select System Devices from the menu bar. Select LiHa from the System Devices menu. Select Dilutor from the LiHa Panel submenu. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 35 If the dilutors are leaking, check the plunger screw and syringe screw for tightness. Also check the valve connections for tightness. Refer to the m1000™ Operating Manual, Section 7.3.2. – Change the syringes and/or 3-way valve. Refer to the m1000™ Operating Manual, Section 7.5. – Repeat the Dilutor tool.

  • Page 36: Door Lock Check

    Select Access Status Options from the Options menu. The Access/Status Panel screen is displayed. Highlight Access Status under Available Devices. Access Status Device Test Select Device Test option under Test. Select the Configuration & Tools tab. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 37 11 If the door lock does not respond to the command, repeat the above step. If the door lock still does not respond, contact your local Abbott representative. 12 Continue to check the door lock function by selecting Start from the Access/Status Panel. Start m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 38 Select <OK> if the left door lock is unlocked. Select Cancel is the left door lock is still locked. Repeat. If the left door lock is still locked, contact your local Abbott representative. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 39 Select <OK> if the right door lock is unlocked. Select Cancel if the right door lock is still locked. Repeat. If the right door lock is still locked, contact your local Abbott representative. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 40: Fawa Test

    The height increase is checked and must be within the expected minimum throughput value. Start the GENESIS Setup & Service software by clicking on the TECAN icon. From the GENESIS Setup & Service main screen, select Liquid System from the menu bar. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 41 Select FaWa from the Liquid System menu. Select Test from the FaWa submenu. From the Select Worktable screen, select NAT MBS Worktable and then <OK>. NAT MBS Worktable The prompt Update instrument settings is displayed. The Fast Wash Pump Test Configuration screen is displayed. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 42 Select 1 for the DiTi start position. Select More. The Fast Wash Pump Test-Adv. Configuration screen is displayed. Complete the following fields: Field Selection User defined Name of user defined test FaWa Duration Minimal throughput to pass tests m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 43 The FaWa Test will pass when the Throughput value is > the minimal throughput value displayed under the Tip column. If the Throughput value is < the minimal throughput value, then the test will fail. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 44: Liquid Detection Test

    Refer to the m1000™ Operating Manual, Section 7.3.2. Exit the GENESIS Setup & Service. Start NAT Wizard and perform a Flush System and look for air bubbles. Refer to the m1000™ Operating Manual, Section 7.3.6. Repeat the Fast Wash Pump test. If the test fails again, contact your local Abbott representative.
  • Page 45 DiTi Rack Select DiTi.RR DiTi Waste Rack Select W1.DiTiWaste First DiTi Position Enter 1 for the destination start point if the DiTi rack if full or enter where to start picking up DiTis. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 46 13 The operator is then prompted Repeat flush step? If any air bubbles remain in the lines, select Yes. If the lines do not have air bubbles, then No can be selected and the Liquid Detection Test will begin. m1000™ Operating Manual Addendum, 392813, en, V1.1...

  • Page 47: Lower Diti Eject Test

    Pass/Fail Criteria The Liquid Detection Test fails: Check if tips are the same height. Check DiTi cones are tight and clean. Refer to the m1000™ Operating Manual, Section 7.3.2. Check the liquid system for air bubbles. Refer to the m1000™ Operating Manual, Section 7.3.2.
  • Page 48 Select DiTi from the Liquid System submenu, then select Lower DiTi Eject. From the Select Worktable screen, select NAT MBS Worktable and then <OK>. NAT MBS Worktable The prompt Update instrument settings is displayed. Next, the Lower DiTi Eject Test screen is displayed. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 49 Once the test is finished, select Close. Pass/Fail Criteria If the Lower DiTi Eject Test fails: Inspect and clean the DiTi cones. Refer to the m1000™ Operation Manual, Section 7.3.2. Check the DiTi adapter. Refer to the m1000™ Operation Manual, Section 7.3.2.

  • Page 50: Posid Test

    From the GENESIS Setup & Service main screen, select System Devices from the menu bar. Select PosID from the System Devices menu. Select Test from the PosID submenu. The PosID2 Test Configuration screen opens. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 51 Select Run to start the test. The PosID2 Test Results window is displayed. Select Start to begin the procedure. The PosID2 Test Results window is displayed. During the test, the values in the PosID2 Test Results window are continuously updated. m1000™ Operating Manual Addendum, 392813, en, V1.1...
  • Page 52 If the PosID Test fails: Check the positions of the barcode labels. Clean the laser beam output window of the PosID. Refer to the m1000™ Operating Manual, Section 7.4.4. Repeat the PosID test. If the test fails again, contact your local Abbott representative.

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