Absorbance Accuracy; Absorbance Wavelength Accuracy - tecan Infinite 200 PRO Instructions For Use Manual

7. Quality Control
Calculation of the Sensitivity (Detection Limit):
7.3.6

Absorbance Accuracy

7.3.7

Absorbance Wavelength Accuracy

Measurement Parameters:
Material/Reagents:
Calculation of Wavelength Accuracy:
170
DetectionL imit ( fmol
-8
2*10 Concentration of ATP standard [M]
Stdev Standard deviation of Blank
B
mean Average of wells filled with ATP standard
ATP
mean Average of Blank wells
B
0.0002 Conversion into mol/well
-15
1/1e Conversion into fmol/well
The result of the formula determines the detection limit in fmol/well.
Use MultiCheck Plate – For details please refer to the MultiCheck Instructions for
Use.
The wavelength accuracy defines the deviation of the set measurement
wavelengths from the nominal wavelength. This test is only valid for the Infinite
M200 PRO.
Parameter
Reading Mode
Measurement Wavelength from/to
Step Size
Number of flashes
Settle Time
Plate Type
MultiCheck plate
Please refer to the data sheet in the instructions for use for your MultiCheck plate.
Wavelength Accuracy
Max theoretical maximum
t
Max measured maximum
m
IFU for Infinite 200 PRO
⋅ −
8
2 10 * 3 *
=
/ well )
−
mean mean
ATP
Setting
Absorbance Scan
300 – 850 nm
1 nm
25
0
MultiCheck plate
= −
Max Max
t m
No. 30052730 Rev. No. 1.6
Stdev
B
* . 0 0002 *
e 1
B
1
−
15
2016-04